Spatial Analysis of 3′ Phosphoinositide Signaling in Living Fibroblasts, III: Influence of Cell Morphology and Morphological Polarity

To perform the vital functions of motility and division, cells must undergo dramatic shifts in cell polarity. Recent evidence suggests that polarized distributions of phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate, which are clearly important for regulating cell morphology during migration, also play an important role during the final event in cell division, which is cytokinesis. Thus, there is a critical interplay between the membrane phosphoinositides and the cytoskeletal cortex that regulates the complex series of cell shape changes that accompany these two processes.

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Spatial Analysis of 3′ Phosphoinositide Signaling in Living Fibroblasts: II. Parameter Estimates for Individual Cells from Experiments

Biophysical Journal ◽

10.1016/s0006-3495(04)74138-7 ◽

2004 ◽

Vol 86 (1) ◽

pp. 599-608 ◽

Cited By ~ 30

Author(s):

Ian C. Schneider ◽

Jason M. Haugh

Keyword(s):

Spatial Analysis ◽

Parameter Estimates ◽

Phosphoinositide Signaling

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Spatial Analysis of 3′ Phosphoinositide Signaling in Living Fibroblasts: I. Uniform Stimulation Model and Bounds on Dimensionless Groups

Biophysical Journal ◽

10.1016/s0006-3495(04)74137-5 ◽

2004 ◽

Vol 86 (1) ◽

pp. 589-598 ◽

Cited By ~ 21

Author(s):

Jason M. Haugh ◽

Ian C. Schneider

Keyword(s):

Spatial Analysis ◽

Phosphoinositide Signaling ◽

Dimensionless Groups

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Author response for "Differential temporal and spatial post‐injury alterations in cerebral cell morphology and viability"

10.1002/cne.24955/v3/response1 ◽

2020 ◽

Author(s):

Zareen Amtul ◽

Abdullah N. Najdat ◽

David J. Hill ◽

Edith J. Arany

Keyword(s):

Cell Morphology ◽

Author Response ◽

Post Injury ◽

Temporal And Spatial

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Cell morphology, volume, and surface area determinations from multilevel contouring within HVEM micrographs of serial sections and whole-cell mounts

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100127426 ◽

1987 ◽

Vol 45 ◽

pp. 588-589

Author(s):

M. Marko ◽

A. Leith ◽

D. Parsons

Keyword(s):

Surface Area ◽

Electron Micrographs ◽

Cell Morphology ◽

Individual Variation ◽

Serial Section ◽

Stereo Pair ◽

Complex Structures ◽

Serial Sections ◽

Surface Areas ◽

Computer Based

The use of serial sections and computer-based 3-D reconstruction techniques affords an opportunity not only to visualize the shape and distribution of the structures being studied, but also to determine their volumes and surface areas. Up until now, this has been done using serial ultrathin sections.The serial-section approach differs from the stereo logical methods of Weibel in that it is based on the Information from a set of single, complete cells (or organelles) rather than on a random 2-dimensional sampling of a population of cells. Because of this, it can more easily provide absolute values of volume and surface area, especially for highly-complex structures. It also allows study of individual variation among the cells, and study of structures which occur only infrequently.We have developed a system for 3-D reconstruction of objects from stereo-pair electron micrographs of thick specimens.

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The organization of cell surface membrane domains

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100155992 ◽

1989 ◽

Vol 47 ◽

pp. 804-805

Author(s):

Michael Edidin

Keyword(s):

Cell Surface ◽

Membrane Lipids ◽

Surface Membrane ◽

Lateral Diffusion ◽

Cell Types ◽

Membrane Domains ◽

Membrane Biology ◽

Morphological Polarity ◽

Discrete Domains ◽

Membrane Components

Cell surface membranes are based on a fluid lipid bilayer and models of the membranes' organization have emphasised the possibilities for lateral motion of membrane lipids and proteins within the bilayer. Two recent trends in cell and membrane biology make us consider ways in which membrane organization works against its inherent fluidity, localizing both lipids and proteins into discrete domains. There is evidence for such domains, even in cells without obvious morphological polarity and organization [Table 1]. Cells that are morphologically polarised, for example epithelial cells, raise the issue of membrane domains in an accute form.The technique of fluorescence photobleaching and recovery, FPR, was developed to measure lateral diffusion of membrane components. It has also proven to be a powerful tool for the analysis of constraints to lateral mobility. FPR resolves several sorts of membrane domains, all on the micrometer scale, in several different cell types.

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