THE URINARY EXCRETION OF 3α,17α,21-TRIHYDROXY-5β-PREGNAN-20-ONE (TETRAHYDRO S) FOLLOWING ADMINISTRATION OF CORTICOTROPHIN AND METOPIRONE (CIBA SU 4885)

ABSTRACT The results of simultaneous measurements of urinary 17-ketogenic steroids and 3α,17α,21-trihydroxy-5β-pregnan-20-one (tetrahydro S) are reported from patients without any endocrine disorders following administration for 3 days of adrenocorticotrophic hormone (ACTH) (15 patients) and oral administration of Metopirone (9 patients). The data obtained are compared with similar determinations performed in 2 patients with Cushing's syndrome, 1 patient with adrenogenital syndrome, 4 patients with hirsutism, 6 patients with suspected pituitary failure and 2 patients with absence of pituitary function. In the patients with no endocrine disorder, tetrahydro S increases fourfold on ACTH stimulation, and almost 60 times following Metopirone administration. The very magnitude of the relative increase in tetrahydro S excretion in normal subjects given Metopirone allows a better gradation of subnormal responses of pituitary ACTH than can be obtained when the effect of the test is judged by the excretion of 17-ketogenic steroids alone. Examples illustrating this are presented in the paper.

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THE EFFECTS OF ACTH AND HCG ON THE URINARY EXCRETION OF TESTOSTERONE IN MALE PATIENTS WITH VARIOUS ENDOCRINE DISORDERS

Acta Endocrinologica ◽

10.1530/acta.0.0530061 ◽

1966 ◽

Vol 53 (1) ◽

pp. 61-72 ◽

Cited By ~ 30

Author(s):

J. Tamm ◽

M. Apostolakis ◽

K. D. Voigt

Keyword(s):

Urinary Excretion ◽

Adrenal Cortex ◽

Normal Subjects ◽

Normal Adult ◽

Endocrine Disorders ◽

List Type ◽

Adult Males ◽

Male Patients ◽

Secondary Hypogonadism

ABSTRACT The effects of HCG and/or ACTH administration have been investigated in 2 normal subjects and in 12 male patients suffering from various endocrinopathies. It was found that: In normal adult males 3000 IU HCG given daily over three days appear to be sufficient to obtain a significant increase in urinary testosterone excretion. The degree of the increase appears to be dependent on the age of the patient. Prolonged HCG administration in secondary hypogonadism can lead to significant sustained increases of testosterone and epitestosterone excretions. Endogenous HCG of the type produced by chorionepitheliomas does not necessarily have an effect on testosterone and epitestosterone production in male patients. HCG stimulates the testosterone and epitestosterone secretion of the testes only; it thus has no effect on orchiectomized patients. Exogenous ACTH increases the testosterone and epitestosterone production of the adrenal cortex, the latter apparently more than the former; the testosterone/epitestosterone quotient in the urine falls. In orchiectomized patients ACTH administration leads to an increase of oestrogen production from the adrenal cortex and of oestriol excretion in the urine.

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17-KETOSTEROID FRACTIONATION STUDIES BY A MICRO-CHROMATOGRAPHIC TECHNIQUE IN ENDOCRINE DISORDERS

Journal of Endocrinology ◽

10.1677/joe.0.0100202 ◽

1954 ◽

Vol 10 (3) ◽

pp. 202-211 ◽

Cited By ~ 14

Author(s):

M. HELEN POND

Keyword(s):

Clinical Medicine ◽

Normal Subjects ◽

Adsorption Chromatography ◽

Endocrine Disorders ◽

Chromatographic Technique ◽

Endocrine Disorder ◽

Adrenocortical Activity ◽

Diagnostic Importance

SUMMARY Urinary 17-ketosteroids have been fractionated by adsorption chromatography on alumina into eight fractions, the main constituents of which are described. The patterns of 17-ketosteroid excretion in patients with various forms of endocrine disorder have been compared with those from normal subjects. The value of this investigation in clinical medicine is discussed. Although not of diagnostic importance, it is of use in the assessment of two aspects of adrenocortical activity, the excretion of the β-hydroxy-17-ketosteroids and the 11-oxy-17-ketosteroids.

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EFFECT OF ADRENOCORTICOTROPHIC HORMONE AND ANDROST-5-EN-17-ON-3β-YL SULPHATE ADMINISTRATION TO THE MOTHER ON URINARY OESTROGEN EXCRETION IN LATE HUMAN PREGNANCY

Journal of Endocrinology ◽

10.1677/joe.0.0480591 ◽

1970 ◽

Vol 48 (4) ◽

pp. 591-598 ◽

Cited By ~ 7

Author(s):

J. JEFFERY ◽

G. H. SWAPP ◽

G. R. WILSON ◽

K. FOTHERBY

Keyword(s):

Urinary Excretion ◽

Normal Subjects ◽

Normal Pregnancy ◽

Adrenal Function ◽

Adrenocorticotrophic Hormone ◽

Human Pregnancy ◽

Variable Pattern

SUMMARY Infusion into the mother of adrenocorticotrophin during late normal pregnancy was accompanied by rises or falls in the urinary excretion of oestrone, oestradiol and oestriol. A similar variable pattern was found when the subjects infused were receiving regular pharmacological doses of glucocorticosteroid (i.e. when maternal adrenal function was reduced) or were carrying anencephalic foetuses (i.e. when foetal adrenal function was reduced). Similar infusion of a substantial quantity of androst-5-en-17-on-3β-yl sulphate (DHAS) was accompanied by rises in oestrogen excretion by normal subjects. The rises were large compared with basal levels, but small (a few per cent) compared with the amount of DHAS infused. The changes in a pregnancy with anencephaly were within the range of the normal subjects. It is concluded that maternal DHAS plays little part in placental oestrogen synthesis, and that some control of oestrogen synthesis from DHAS occurs by the placenta itself.

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Pharmacokinetics and urinary excretion of orally administered diiodotyrosine

Acta Endocrinologica ◽

10.1530/acta.0.1160395 ◽

1987 ◽

Vol 116 (3) ◽

pp. 395-398

Author(s):

H. Meinhold ◽

R. Finke ◽

U. Bogner ◽

H. Schleusener

Keyword(s):

Gastrointestinal Tract ◽

Oral Administration ◽

Urinary Excretion ◽

Normal Subjects ◽

Serum Levels ◽

Time Interval ◽

Rapid Absorption ◽

Metabolic Degradation ◽

Excretion Rates

Abstract. Serum levels of diiodotyrosine (DIT) and urinary excretion rates of DIT and iodine were measured in 10 normal subjects after oral administration of 1.57 μmol of DIT corresponding to 400 μg of iodine. Serum DIT concentrations rose promptly from a mean endogenous basal level of 0.23 nmol/l to maximum values between 6.0 and 20 nmol/l within 30 min to 1 h after DIT ingestion. Decreasing DIT levels were found in all subjects 2 h after DIT intake. Urinary excretion of intact DIT was low, being less than 1% of the administered dose of exogenous DIT within 2 days. In contrast, 52% of the iodine administered in the form of DIT was excreted in the urine in the same time interval. The rapid absorption of DIT from the gastrointestinal tract combined with rapid and almost complete metabolic degradation by deiodination make orally applied DIT seem a suitable iodine carrier compound for therapeutic purposes.

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ÉTUDES IN VIVO SUR LE MÉTABOLISME DES ANDROGÉNES APRES ADMINISTRATION DE STÉROÏDES INHIBITEURS DE L'OVULATION

Acta Endocrinologica ◽

10.1530/acta.0.0500131 ◽

1965 ◽

Vol 50 (1) ◽

pp. 131-144 ◽

Cited By ~ 1

Author(s):

P. Mauvais-Jarvis ◽

M. F. Jayle ◽

J. Decourt ◽

J. Louchart ◽

J. Truffert

Keyword(s):

Luteinizing Hormone ◽

Urinary Excretion ◽

Normal Subjects ◽

Hormone Activity ◽

Testosterone Production ◽

Normal Men ◽

Ethinyl Oestradiol

ABSTRACT Normal subjects and hirsute women with micropolycystic ovaries were treated with ethinyl-oestrenol + 3-methoxy-ethinyl-oestradiol (Lyndiol®), in view of studying the action of this compound on the production of androgens and on the urinary excretion of their metabolites. In normal men, the production of testosterone and the excretion of androsterone and aetiocholanolone are suppressed, whereas the excretion of other 17-ketosteroids and the production of dehydroepiandrosterone sulphate are unchanged. Moreover, the luteinizing hormone activity (LH) in plasma is depressed. It seems that the preparation inhibits specifically the testicular androgen production, by suppressing the hypothalamo-hypophyseal control of LH. Testosterone production and urinary 17-ketosteroid excretion are modified in the same way in women with Stein-Leventhal's syndrome. Physiopathological and therapeutical implications which come from these results are discussed.

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A COMPARISON OF TWO METHODS FOR MEASUREMENT OF ALDOSTERONE SECRETION RATE IN MAN

Acta Endocrinologica ◽

10.1530/acta.0.0530177 ◽

1966 ◽

Vol 53 (2) ◽

pp. 177-188 ◽

Cited By ~ 4

Author(s):

P. Lund-Johansen ◽

T. Thorsen ◽

K. F. Støa

Keyword(s):

Urinary Excretion ◽

Normal Subjects ◽

Secretion Rate ◽

Aldosterone Secretion ◽

Simple Method ◽

Mean Values ◽

Pathological Conditions ◽

Significant Difference ◽

Derivative Method ◽

The Mean

ABSTRACT A comparison has been made between (A), a relatively simple method for the measurement of aldosterone secretion rate, based on paper chromatography and direct densitometry of the aldosterone spot and (B) a more elaborate isotope derivative method. The mean secretion rate in 9 normal subjects was 112 ± 26 μg per 24 hours (method A) and 135 ± 35 μg per 24 hours (method B). The »secretion rate« in one adrenalectomized subject after the intravenous injection of 250 μg of aldosterone was 230 μg per 24 hours (method A) and 294 μg per 24 hours (method B). There was no significant difference in the mean values, and correlation between the two methods was good (r = 0.80). It is concluded that the densitometric method is suitable for clinical purposes as well as research, being more rapid and less expensive than the isotope derivative method. Method A also measures the urinary excretion of the aldosterone 3-oxo-conjugate, which is of interest in many pathological conditions. The densitometric method is obviously the less sensitive and a prerequisite for its use is an aldosterone secretion of 20—30 μg per 24 hours. Lower values are, however, rare in adults.

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ATYPICAL ADRENAL HYPERFUNCTION: IN VIVO STUDIES AND INCUBATIONS OF ADRENAL TISSUE WITH 4-14C PROGESTERONE

Acta Endocrinologica ◽

10.1530/acta.0.058s005 ◽

1968 ◽

Vol 58 (3_Suppl) ◽

pp. S5-S34

Author(s):

Joseph W. Goldzieher ◽

Leonard R. Axelrod ◽

Arthur S. Weissbein

Keyword(s):

Urinary Excretion ◽

Clinical Features ◽

In Vivo Studies ◽

Acth Stimulation ◽

Adrenal Tissue ◽

Adrenal Hyperfunction

ABSTRACT Six women with atypical forms of adrenal cortical hyperfunction were studied by means of urinary excretion of 17-ketosteroids and 17-hydroxycorticoids and their response to ACTH stimulation and corticosteroid suppression. Unusual responses were observed, particularly with respect to the independence of 17-KS and 17-OHCS excretion. The adrenals of 3 patients were anatomically normal whereas the others showed hyperplasia. Minced adrenal tissue was incubated with 4-14C progesterone and the metabolites isolated and definitively identified. The pattern of biosynthesized corticosteroids showed great variation, and in some instances clarified certain clinical features. The pattern of certain C19-metabolites could not be studied adequately because of the use of a Δ4 rather than a Δ5 substrate.

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