URINARY EXCRETION OF PREGNANETRIOL IN THE MENSTRUAL CYCLE. I

1962 ◽  
Vol 41 (1) ◽  
pp. 129-134 ◽  
Author(s):  
Mary T. Pickett ◽  
A. E. Kellie
Keyword(s):  
Infrared Spectroscopy ◽  
Menstrual Cycle ◽  
Urinary Excretion ◽  
Luteal Phase ◽  
Healthy Woman ◽  
Gradient Elution ◽  
Urine Samples ◽  
Partition Chromatography ◽  
Ovulatory Cycle ◽  
Elution Chromatography

ABSTRACT Using the method of Stern (1957), pregnanetriol fractions have been prepared from urine samples of a healthy woman during phases of minimal and maximal pregnanetriol excretion. Examination of these fractions by bismuthate oxidation and by gradient elution chromatography has confirmed that the increase measured by the Stern method is, indeed, mainly due to 5β-pregnane-3α,17α,20α-triol. A similar fraction obtained during the luteal phase of an ovulatory cycle in an adrenalectomized woman has been purified by adsorption and partition chromatography and shown by infrared spectroscopy to contain 5β-pregnane-3α,17α,20α-triol.

Urinary melatonin, LH, oestradiol, progesterone excretion during the menstrual cycle or in women taking oral contraceptives

1987 ◽  
Vol 116 (1) ◽  
pp. 145-149 ◽  
Author(s):  
Jocelyne Brun ◽  
Bruno Claustrat ◽  
Michel David
Keyword(s):  
Oral Contraceptive ◽  
Menstrual Cycle ◽  
Urinary Excretion ◽  
Oral Contraceptives ◽  
Coefficient Of Variation ◽  
Luteal Phase ◽  
Positive Relationship ◽  
Follicular Phase ◽  
The Other ◽  
Normal Women

Abstract. Nocturnal urinary excretion of melatonin, LH, progesterone and oestradiol was measured by radioimmunoassay in nine normal women during a complete cycle. In addition, these hormonal excretions were studied in two women taking an oral contraceptive. A high within-subject coefficient of variation was observed for melatonin excretion in the two groups. In the nine normal cycling women, melatonin excretion was not decreased at the time of ovulation, but was significantly increased during the luteal phase compared with that of the follicular phase (P < 0.01). These data are consistent with a positive relationship between melatonin and progesterone during the luteal phase. In the two women under an oral contraceptive, melatonin excretion was found within the same range as for the other nine. The results are discussed in terms of pineal investigation in human.

scholarly journals Correlation between endometrial dating of luteal phase days 6 and 10 of the same menstrual cycle

1998 ◽  
Vol 116 (3) ◽  
pp. 1734-1737 ◽  
Author(s):  
Jorge Haddad Filho ◽  
Agnaldo Pereira Cedenho ◽  
Vilmon de Freitas
Keyword(s):  
Menstrual Cycle ◽  
Luteal Phase ◽  
Endometrial Thickness ◽  
Human Reproduction ◽  
Progesterone Level ◽  
Ovulatory Cycle ◽  
Plasma Progesterone ◽  
Menstrual Cycles ◽  
Infertile Couples ◽  
Study Setting

CONTEXT: Endometrial maturation, important in the diagnosis of infertile couples, has been evaluated since 1950 using the Noyes criteria. Nevertheless, there is no consensus regarding the most suitable period of the luteal phase for performing the biopsy. OBJETIVE: This study evaluated the correlation between the histological dating of two endometrial biopsies performed in the same menstrual cycle, on luteal phase days six and ten. DESIGN: Prospective study. SETTING: Human Reproduction Division of the Federal University of São Paulo, referral center. PATIENTS:Twenty-five women complaining of infertility had their menstrual cycles monitored by ultrasound and LH plasma levels, to obtain evidence of ovulation. PROCEDURES: Endometrial biopsies were performed on luteal phase days LH+6 and LH+10 (luteal phase day 1 = LH+1 = the day that follows LH peak). Dating was done according to morphometric criteria, in which an endometrium sample is considered out of phase if the minimum maturation delay is one day. On day LH+6, blood was drawn for plasma progesterone level determination. RESULTS: All patients had an ovulatory cycle (mean LH peak: 47.4 U/L; mean follicular diameter on LH peak day: 18.9 mm; mean endometrial thickness on LH peak day: 10.3 mm; mean plasma progesterone level on day LH+6: 14.4 ng/ml). 14 patients had both biopsies in phase; 5 patients had out of phase biopsies only on day LH+6; 3 had out of phase biopsies only on day LH+10 and 3 patients had out of phase biopsies on both days. McNemar's test showed no statistical difference between these data (p>33.36%). CONCLUSIONS: The correlation found between the endometrial datings suggests that biopsies performed on either of these two days are suitable for evaluation of endometrial maturation.

Indices of ovulation: comparison of plasma and salivary levels of progesterone with urinary pregnanediol

1984 ◽  
Vol 100 (1) ◽  
pp. 75-80 ◽  
Author(s):  
M. G. Metcalf ◽  
J. J. Evans ◽  
J. A. Mackenzie
Keyword(s):  
Menstrual Cycle ◽  
Luteal Phase ◽  
Follicular Phase ◽  
The Other ◽  
Urine Samples ◽  
Median Increase ◽  
Daily Excretion ◽  
Alternative Procedures ◽  
Biochemical Assessment

ABSTRACT An increased daily excretion of pregnanediol, relative to that early in the menstrual cycle, is often taken to be evidence that a woman has ovulated. This paper assesses the value of alternative procedures for this purpose. Urine, plasma and saliva samples were collected during a 24-h period from 20 women during the follicular phase and from 20 women during the luteal phase. The 24-h excretion of pregnanediol was compared with (1) the concentration of progesterone in plasma, (2) the concentration of progesterone in saliva, (3) the concentration of pregnanediol in small urine samples, (4) the rate of excretion of pregnanediol and (5) the ratio of pregnanediol to creatinine in small urine samples. Each analyte increased substantially during the luteal phase. The median increases (ratio of luteal to follicular phase values) were 14·8, 3·2, 10·6, 11·9 and 11·1 respectively. By comparison, the median increase in 24-h pregnanediol output was 9·2. When the other analytes were used instead of the 24-h excretion of pregnanediol to assess the possibility of ovulation, the incidence of misclassifications (follicular samples classed as luteal and luteal samples classed as follicular) was 0, 12·8, 5·9, 2·0 and 1·0% respectively. It was concluded that the most satisfactory alternative to the measurement of 24-h pregnanediol output for the biochemical assessment of ovulation based on progesterone production was the measurement of the concentration of progesterone in plasma; the least satisfactory alternative was determination of the concentration of progesterone in saliva. If blood was not available, measurement of the ratio of pregnanediol to creatinine in a small urine sample was the preferred method. J. Endocr. (1984) 100, 75–80

THE URINARY EXCRETION OF OESTROGENS, PREGNANEDIOL AND GONADOTROPHINS DURING THE MENSTRUAL CYCLE

1958 ◽  
Vol 17 (4) ◽  
pp. 401-410 ◽  
Author(s):  
J. B. BROWN ◽  
A. KLOPPER ◽  
J. A. LORAINE
Keyword(s):  
Menstrual Cycle ◽  
Urinary Excretion ◽  
Artificial Insemination ◽  
Luteal Phase ◽  
Mouse Uterus ◽  
Marked Rise ◽  
Menstrual Cycles ◽  
Hormone Excretion

SUMMARY 1. The urinary excretion of oestrogens, pregnanediol and pituitary gonadotrophins has been studied throughout nine ovulatory menstrual cycles. 2. The pattern of hormone excretion was relatively constant from one individual to another, but the actual amounts excreted varied considerably in different individuals. 3. In none of the subjects studied did the mid-cycle peak in gonadotrophin precede the oestrogen peak. 4. The increase in urinary pregnanediol during the luteal phase occurred at the same time as or just before the rise in basal temperature and 1–4 days after the oestrogen peak. 5. There was no correlation between the amounts of oestrogens and pregnanediol excreted during the luteal phase of the cycle. 6. When gonadotrophin assays were conducted by the mouse uterus test and that depending on the prostate of the hypophysectomized rat, the results obtained agreed very closely at all stages of the cycle. 7. In one subject a marked rise in gonadotrophin output was observed as early as 9 days after a successful artificial insemination.

URINARY EXCRETION OF PREGNANETRIOL IN THE MENSTRUAL CYCLE. II

1962 ◽  
Vol 41 (1) ◽  
pp. 135-142 ◽  
Author(s):  
Mary T. Pickett ◽  
Ian F. Sommerville
Keyword(s):  
Menstrual Cycle ◽  
Urinary Excretion ◽  
Sulphuric Acid ◽  
Standard Method ◽  
Cyclic Change ◽  
Ovulatory Cycle ◽  
Menstrual Cycles ◽  
Urinary Steroids ◽  
Acid Reaction ◽  
Temperature Records

ABSTRACT The pattern of urinary pregnanetriol excretion is compared with that of other urinary steroids assayed daily throughout the menstrual cycles of four healthy women. Evidence for the occurrence and estimated date of ovulation is afforded by basal temperature records, urinary oestrogen and pregnanediol excretion and cytology of anterior urethral cells recovered from the urine. In addition, pregnanediol and pregnanetriol excretion has been studied for twelve days in an ovariectomised subject. It is found that the cyclic change in urinary pregnanetriol excretion is variable in extent but there is a correlation between the urinary excretion of oestrogens, pregnanediol and pregnanetriol during the ovulatory cycle. Modification of the programme of elution used in the standard method does not significantly affect the result as determined by the sulphuric acid reaction.

URINARY EXCRETION OF ANDROSTENEDIONE, TESTOSTERONE, EPITESTOSTERONE AND DEHYDROEPIANDROSTERONE DURING THE NORMAL MENSTRUAL CYCLE

1968 ◽  
Vol 59 (4) ◽  
pp. 644-651 ◽  
Author(s):  
N. Longhino ◽  
M. Tajić ◽  
M. Vedriš ◽  
D. Janković ◽  
P. Drobnjak
Keyword(s):  
Menstrual Cycle ◽  
Urinary Excretion ◽  
Luteal Phase ◽  
Normal Menstrual Cycle

ABSTRACT The urinary excretion of total androstenedione i. e. free, glucosiduronide and sulphate, testosterone and dehydroepiandrosterone during two normal menstrual cycle is presented. Epitestosterone was determinated during one cycle only. Both cycles were ovulatory as can be seen from the pregnanediol and oestriol determinations. The androstenedione and testosterone excretion values showed a caracteristic decrease from 12th to 16th day and an increase during the luteal phases, reaching their maximal levels between the 20th and 25th (cycle A) and 20th and 24th day (cycle B) respectively. The excretion values of epitestosterone were highest on 22nd and 23rd day. Dehydroepiandrosterone values were also slightly raised during the luteal phase of the cycle.

SOME OBSERVATIONS ON THE URINARY EXCRETION OF TESTOSTERONE DURING THE NORMAL MENSTRUAL CYCLE

1968 ◽  
Vol 58 (4) ◽  
pp. 685-695 ◽  
Author(s):  
A. A. A. Ismail ◽  
R. A. Harkness ◽  
J. A. Loraine
Keyword(s):  
Menstrual Cycle ◽  
Urinary Excretion ◽  
Luteal Phase ◽  
Broad Band ◽  
Follicular Phase ◽  
Normal Menstrual Cycle ◽  
Menstruating Women ◽  
Steroid Excretion

ABSTRACT Serial assays of urinary testosterone have been performed in seven normally menstruating women; in six of these pregnanediol estimations were also conducted and in four oestrogen determinations were made. All the cycles were of an ovulatory character as judged by the pattern of steroid excretion. In five subjects peaks of testosterone output were observed in the luteal phase of the cycle. In one a broad band of excretion extended from midcycle into the luteal phase, and in one the luteal peak was absent. Four women showed definite peaks of testosterone output at midcycle, and in two follicular phase peaks were also encountered. It is suggested that fluctuations in urinary testosterone excretion during the normal menstrual cycle result from the secretion of precursors of the hormone by the ovaries rather than by the adrenals.

PREGNANETRIOL, PREGNANEDIOL AND OESTROGEN EXCRETION DURING ANOVULATORY MENSTRUAL CYCLES AND IN A PREMENOPAUSAL ADRENALECTOMIZED WOMAN

1964 ◽  
Vol 29 (1) ◽  
pp. 55-60 ◽  
Author(s):  
K. FOTHERBY ◽  
J. B. BROWN
Keyword(s):  
Menstrual Cycle ◽  
Urinary Excretion ◽  
Ovulatory Cycle ◽  
Menstrual Cycles ◽  
Anovulatory Cycle

SUMMARY The urinary excretion of pregnanetriol, pregnanediol and oestrogens was measured throughout five anovulatory cycles in three subjects, and throughout an anovulatory cycle and an ovulatory cycle in an adrenalectomized subject. Cyclical changes in pregnanetriol excretion, which paralleled the changes in oestrogen output, were observed during some of the anovulatory cycles and also during the ovulatory cycle in the adrenalectomized subject. These findings suggest that one factor contributing to the increase in pregnanetriol excretion during the normal ovulatory menstrual cycle is the secretion by the ovary, at times of maximal oestrogen secretion, of a precursor (possibly 17α-hydroxyprogesterone) common to both oestrogens and pregnanetriol. However, that other factors are probably also involved is suggested by the particular pattern of pregnanetriol excretion during the normal ovulatory cycle and by the finding that the increase in pregnanetriol excretion at about the time of ovulation is usually greater than the increase found in the anovulatory cycle.

EVALUATION OF CHANGES IN CARDIORESPIRATORY FUNCTIONS DURING DIFFERENT PHASES OF THE MENSTRUAL CYCLE IN YOUNG WOMEN

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Shehnaz Shaikh
Keyword(s):  
Menstrual Cycle ◽  
Sample Size ◽  
Young Women ◽  
Luteal Phase ◽  
Uterine Wall ◽  
Normal Weight ◽  
Follicular Phase ◽  
Menstrual Phase ◽  
Granulose Cells ◽  
Efficiency Test

Introduction: Menstrual cycle or menstruation involved discharge of sanguinous fluid and a sloughing of uterine wall. In women menstruation occurs at regular intervals on an average of 28 days, although most women gave a history of regular intervals of 28 to 30 days. About 10% -15% of women showed cycle at the precise 28 ± 2 days intervals when menstrual calendar was utilized. Normally in young women in different phases of ovarian cycles the plasma levels of estrogen vary. Ovulation occurs in the first 12-13th day of menstrual cycle, which is termed estrogen surge and second occurs in mid-luteal phase. During mid cycle or follicular phase of menstrual cycle the plasma concentration of progesterone is very low about 0.9 ng/mL. its level starts rising owing to secretion from the granulose cells. During luteal phase progesterone level reaches its peak value of 18 ng/mL and its level fall to a minimum value toward the end of the cycle. Estrogen affects local and systemic vasodilation. The menstrual cycle envelops two fundamental stages, the follicular stage (FP) and the luteal stage (LP). The follicular stage can part advance into two substages; the early FP, which is characterised with moo concentrations of both the key hormones estrogen and progesterone; and the mid FP where estrogen is tall autonomously from progesterone. The LP is epitomized by tall concentration of both estrogen and progesterone. These two fundamental stages are isolated by a soak surge in luteinizing hormone activating ovulation. These recurrent changes are said to be frequency unsurprising while long time. Aim: The main aim of this study is to evaluate the Cardiorespiratory functions changes during different Phases of Menstrual Cycle.   Material and methods: In this study, 20 with normal weight, 20 with obese and 20 with overage were included and taken them as a sample size. In this study all the young women those were recruited as a sample size are unmarried, undergraduate female student with the between the age group of 18-22years, having regular 28+6 days menstrual cycle for at least last 6months prior to this study. For the collection of data all the participants were instructed to attend the physiology lab department during each of three different phases. Day-2 during menstrual phase, Day-7, during follicular phase and Day-22 during luteal phase and the following parameters were recorded as Anthropometric measurements, measuring of pulse rate and blood pressure and cardiac efficiency test. Result: In general, work out proficiency changed essentially amid the distinctive stages of the menstrual cycle with the most elevated amid luteal stage and least amid menstrualo stage. There was no critical contrast in impact test amid menstrual stage, follicular stage and luteal stage of menstrual cycle among three bunches of people. Conclusion: We have watched noteworthy increment in cardiac and respiratory proficiency within the luteal stage of the menstrual cycle in ordinary weight people. Lower wellness levels were watched in overweight and stout females. In this manner hone of customary work out and admissions of solid slim down which offer assistance in lessening the weight and in turn the BMI will offer assistance in improving the physical wellness of the people. Keywords: Cardiorespiratory, Menstrual cycle, expiratory blast test

EFFECT OF LH-RH ON SERUM FSH, LH AND PROGESTERONE DURING THE LUTEAL PHASE OF THE MENSTRUAL CYCLE

1975 ◽  
Vol 80 (1_Suppla) ◽  
pp. S20
Author(s):  
I. Gerhard ◽  
M. Röhrich ◽  
K. Klinga ◽  
B. Runnebaum
Keyword(s):  
Menstrual Cycle ◽  
Luteal Phase ◽  
Lh Rh
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