URINARY EXCRETION OF PREGNANETRIOL IN THE MENSTRUAL CYCLE. II
ABSTRACT The pattern of urinary pregnanetriol excretion is compared with that of other urinary steroids assayed daily throughout the menstrual cycles of four healthy women. Evidence for the occurrence and estimated date of ovulation is afforded by basal temperature records, urinary oestrogen and pregnanediol excretion and cytology of anterior urethral cells recovered from the urine. In addition, pregnanediol and pregnanetriol excretion has been studied for twelve days in an ovariectomised subject. It is found that the cyclic change in urinary pregnanetriol excretion is variable in extent but there is a correlation between the urinary excretion of oestrogens, pregnanediol and pregnanetriol during the ovulatory cycle. Modification of the programme of elution used in the standard method does not significantly affect the result as determined by the sulphuric acid reaction.