SOME BIOLOGICAL PROPERTIES OF A POTENT FOLLICLE STIMULATING HORMONE PREPARATION

1965 ◽  
Vol 48 (3) ◽  
pp. 439-445 ◽  
Author(s):  
Harold Papkoff
Keyword(s):  
Interstitial Cell ◽  
Follicle Stimulating Hormone ◽  
Intrinsic Property ◽  
Biological Properties ◽  
Female Rats ◽  
Male And Female ◽  
Male And Female Rats ◽  
Stimulating Hormone

ABSTRACT A preparation of ovine follicle stimulating hormone (FSH) which is approximately 50 times as active as NIH-FSH-S1 has been tested in hypophysectomized male and female rats and found to be a potent steroidogenic factor. Contamination of the FSH preparation with interstitial cell-stimulating hormone (ICSH, LH) was found to be very low (0.006–0.016 unit/mg) and could not account for the observed activity. In addition, treatment of the FSH preparation with 6 m urea and neuraminidase indicates that the steroidogenic activity is either an intrinsic property of the FSH molecule or a discrete entity which differs chemically from ICSH.

CHANGES IN SECRETION OF LUTEINIZING HORMONE AND FOLLICLE-STIMULATING HORMONE INDUCED BY HYPOVOLAEMIA IN RATS

1981 ◽  
Vol 88 (1) ◽  
pp. 97-101 ◽  
Author(s):  
V. W. K. LEE ◽  
D. M. DE KRETSER
Keyword(s):  
Luteinizing Hormone ◽  
Blood Volume ◽  
Follicle Stimulating Hormone ◽  
Blood Sampling ◽  
Female Rats ◽  
Male And Female ◽  
Male And Female Rats ◽  
Egg Albumen ◽  
Stimulating Hormone

Changes in levels of LH and FSH in the circulation were examined during repeated blood sampling in untreated rats and gonadectomized male and female rats treated with oestrogen, progesterone and thyroxine. Blood depletion induced a significant increase in levels of LH in steroid-treated rats but the increase was abolished when the depleted blood volume was replaced with egg albumen. The rise in LH was less dramatic in male than in female animals. In untreated rats, levels of LH either decreased or did not change with repeated phlebotomy. In contrast, the levels of FSH either did not alter or were lowered in all situations.

STUDIES ON THE TESTICULAR AUGMENTATION ASSAY METHOD FOR FOLLICLE STIMULATING HORMONE

1966 ◽  
Vol 52 (4) ◽  
pp. 573-582 ◽  
Author(s):  
E. Gans ◽  
G. P. van Rees
Keyword(s):  
Follicle Stimulating Hormone ◽  
Female Rats ◽  
Assay Method ◽  
Testis Weight ◽  
Promoting Effect ◽  
Male And Female ◽  
Male And Female Rats ◽  
Rat Pituitary ◽  
Pituitary Homogenate ◽  
Stimulating Hormone

ABSTRACT Augmentation of testis weight caused by the follicle stimulating hormone (FSH) was studied in hypophysectomized immature rats treated concomitantly with supramaximal amounts of human chorionic gonadotrophin (HCG). Using a rat pituitary homogenate, responses were found to increase linearly with the logarithm of the doses given. In assays of rat pituitary homogenate against purified sheep FSH no significant divergence was observed. The effect of a submaximally active dose of FSH was not significantly altered by the concomitant administration of purified growth hormone, thyrotrophin, corticotrophin, prolactin or luteinizing hormone (LH). Serum from intact male and female rats caused significant growth of the testis, while serum from male animals was more active than that of female rats. However, when serum from hypophysectomized male and female rats was given, some testis growth still occurred in one of the two experimental series. In this case, the responses were smaller than those caused by serum from intact males but not smaller than those caused by serum from intact females. No difference in testis growth-promoting effect could be demonstrated between the sera obtained from hypophysectomized male and female rats.

GONADOTROPHIN PATTERNS IN MALE AND FEMALE RATS: PLASMA LH AND FSH AT VARIOUS AGES EVALUATED BY THE METHOD OF PARABIOSIS

1967 ◽  
Vol 56 (1) ◽  
pp. 165-176 ◽  
Author(s):  
Donald C. Johnson
Keyword(s):  
Follicle Stimulating Hormone ◽  
Chorionic Gonadotrophin ◽  
Female Rats ◽  
Adult Males ◽  
Male And Female ◽  
Ovarian Weight ◽  
Average Value ◽  
Male And Female Rats ◽  
Tonic Level ◽  
Males And Females

ABSTRACT Ventral prostates in hypophysectomized male parabiotic partners of intact animals were used to compare the amount of plasma luteinizing hormone (LH) in males and females of various ages. Ovarian weight, histology, and augmentation with chorionic gonadotrophin, in hypophysectomized androgenized females were used to estimate plasma follicle stimulating hormone (FSH) activity in intact adult males and females. In young animals, up to 50 days of age, males apparently have the same amount of plasma LH as females, but older cyclic females produced significantly heavier prostates in their hypophysectomized male partners than did males. The results are consistent with the interpretation that cyclic surges of LH added to a tonic level produced an average value higher for females than males. In contrast, males of all ages and particularly adults, have a significantly greater amount of circulating FSH than females.

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