TREATMENT OF AMENORRHOEA WITH MENOPAUSAL URINARY GONADOTROPHIN EVALUATED BY THIN-LAYER CHROMATOGRAPHIC DETERMINATION OF PREGNANEDIOL IN THE URINE

1966 ◽  
Vol 53 (4) ◽  
pp. 681-686
Author(s):  
Helge G. Berthelsen ◽  
H. O. Bang
Keyword(s):  
Thin Layer ◽  
Clinical Effect ◽  
Chromatographic Determination ◽  
Temperature Curve ◽  
Chorionic Gonadotrophin ◽  
Vaginal Examination ◽  
Abnormal Increase ◽  
Vaginal Cytology ◽  
Local Reactions

ABSTRACT Three young amenorrhoeic hypogonadal women, aged 19-24 years, all nulliparous, with a monophasic basal-temperature curve for at least 2 months, a low hypotrophic vaginal cytology, and a constantly low pregnanediol excretion were treated with an intramuscular injection of human menopausal urinary gonadotrophin (HMG) for ten days, followed by daily injections of chorionic gonadotrophin for four days. All the patients had previously been treated unsuccessfully with chorionic gonadotrophin. The clinical effect was checked by daily thin-layer-chromatographic determination of the pregnanediol excretion in the morning urine, vaginal cytology, and daily recording of the basal temperature. Vaginal examination was done every day. One patient exhibited enlargement of the ovaries during the treatment, but apart from that there were no side effects, in particular no abnormal increase in basal temperature and no local reactions in the treated patients. All the patients developed vaginal bleeding following an increase of the pregnanediol excretion. One woman conceived.

High-performance thin-layer chromatographic determination of rosiglitazone in its dosage form

2002 ◽  
Vol 15 (3) ◽  
pp. 192-195 ◽  
Author(s):  
Ramesh Sane ◽  
Mary Francis ◽  
Atul Moghe ◽  
Sachin Khedkar ◽  
Ajit Anerao
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Dosage Form ◽  
Chromatographic Determination

Improved thin-layer chromatographic determination of phospholipids in gastric aspirate from newborns, for assessment of lung maturity.

1988 ◽  
Vol 34 (4) ◽  
pp. 736-738 ◽  
Author(s):  
D Serrano de la Cruz ◽  
E Santillana ◽  
A Mingo ◽  
G Fuenmayor ◽  
A Pantoja ◽  
...  
Keyword(s):  
Thin Layer ◽  
Fetal Lung ◽  
Chromatographic Determination ◽  
Solvent Mixture ◽  
Gastric Aspirate ◽  
One Dimensional ◽  
Acetic Acid Water ◽  
Chromatographic Resolution ◽  
Lung Maturity

Abstract This one-dimensional thin-layer chromatographic method is used for assay of phospholipids in the gastric aspirate of newborns. The solvent mixture (chloroform/hexane/methanol/glacial acetic acid/water, 12/7/4/3/0.3 by vol) completely resolves lecithin, sphingomyelin, phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, and phosphatidylglycerol. The method is simple, precise, inexpensive, and rapid (chromatographic development takes less than 25 min) and gives high chromatographic resolution. We used this method to determine the lecithin/sphingomyelin densitometric ratio (L/S ratio) and the phosphatidylglycerol percentage in 200 samples of gastric aspirate and found an L/S ratio of 2.5 to be a satisfactory cutoff value for distinguishing fetal lung maturity and immaturity. We confirmed that the presence of phosphatidylglycerol excluded the possibility of respiratory distress.

Quantitative thin-layer chromatographic determination of bromide(1-) residues in crops after soil treatment by methyl bromide

1979 ◽  
Vol 27 (1) ◽  
pp. 132-134 ◽  
Author(s):  
Luc A. Gordts ◽  
Andre Vandezande ◽  
Pieter P. Van Cauwenberge ◽  
Willy Van Haver
Keyword(s):  
Thin Layer ◽  
Methyl Bromide ◽  
Chromatographic Determination ◽  
Soil Treatment

Thin Layer Chromatographic Determination of Subsidiary Dyes in D&C Red No. 19 and D&C Red No. 37

1974 ◽  
Vol 57 (4) ◽  
pp. 961-962
Author(s):  
Sandra Bell
Keyword(s):  
Thin Layer ◽  
Silica Gel ◽  
Chromatographic Method ◽  
Chromatographic Determination

Abstract A thin layer chromatographic method is presented by which triethylrhodamine and other lower ethylated subsidiary colors are separated from D&C Red No. 19 and D&C Red No. 37. After removal from the plate, subsidiary dyes are extracted from silica gel C adsorbent and quantitated spectrophotometrically. Recoveries of 1, 2, and 5% of added triethylrhodamine ranged from 89 to 1 0 3% for D&C Red No. 19 and from 85 to 1 0 2% for D&C Red No. 37.

Rapid Thin Layer Chromatographic Determination of Aflatoxin M1 in Powdered Milk

1985 ◽  
Vol 68 (5) ◽  
pp. 952-954
Author(s):  
Maria Luisa Serralheiro ◽  
Maria Lurdes Quinta
Keyword(s):  
Aqueous Solution ◽  
Carbon Tetrachloride ◽  
Thin Layer ◽  
Limit Of Detection ◽  
Chromatographic Determination ◽  
Methanol Solution ◽  
Aflatoxin M1 ◽  
Powdered Milk ◽  
Layer Chromatography

Abstract A method has been developed for the detection of aflatoxin Mi in milk. The toxin is extracted with chloroform, the extract is evaporated, and the residue is partitioned between carbon tetrachloride and an aqueous saline-methanol solution. The toxin is once again extracted with chloroform from the methanol solution and analyzed by thin layer chromatography. The limit of detection of Mi in powdered milk is 0.5 μg/ kg; recoveries of added Mj are about 83%. The limit of detection can be improved to 0.3 μg/kg if the plate is sprayed with an aqueous solution of H2S04 after development.

Quantitative Thin Layer Chromatographic Determination of Furazolidone and Nitrofurazone in Animal Feeds

1978 ◽  
Vol 61 (1) ◽  
pp. 92-95
Author(s):  
Ugo R Cieri
Keyword(s):  
Thin Layer ◽  
Ultraviolet Light ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Animal Feeds ◽  
Recording Spectrophotometer ◽  
Tungsten Lamp

Abstract A method is presented for determining the nitrofurans furazolidone and nitrofurazone in animal feeds. The sample is extracted with acetone, and aliquots of the concentrated extract are spotted on thin layer chromatographic fluorescent plates. After development in CHCl3-methanol (90+10), the bands containing the nitrofurans are detected under shortwave ultraviolet light, scraped from the plate, and extracted with ethanol. The centrifuged extracts are scanned from 500 to 300 nm on a recording spectrophotometer with a tungsten lamp, and the absorbance maxima near 360 nm are used for quantitation. To compensate for extraction and chromatographic losses and for other interferences, the nitrofuran not present in the sample is added as an internal standard. The method is generally not applicable at a level below 0.005% since detection of the bands becomes difficult.

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