ISOELECTRIC FOCUSING OF IGG CONTAINING LATS, THYROGLOBULIN ANTIBODIES AND THYROID MICROSOMAL ANTIBODIES

1971 ◽  
Vol 68 (1_Supplb) ◽  
pp. S15
Author(s):  
F. Adlkofer ◽  
H. Schleusener ◽  
L. Uher ◽  
H. J. Kirstaedter
Keyword(s):  
Isoelectric Focusing ◽  
Thyroglobulin Antibodies ◽  
Thyroid Microsomal Antibodies

HETEROGENEITY OF LONG ACTING THYROID STIMULATING (LATS) – ACTIVITY, THYROGLOBULIN ANTIBODIES AND THYROID MICROSOMAL ANTIBODIES

1973 ◽  
Vol 73 (3) ◽  
pp. 483-488 ◽  
Author(s):  
F. Adlkofer ◽  
H. Schleusener ◽  
L. Uher ◽  
A. Ananos ◽  
C. Brammeier
Keyword(s):  
Isoelectric Focusing ◽  
Graves Disease ◽  
Thyroid Antibodies ◽  
Thyroglobulin Antibodies ◽  
Ph Range ◽  
Long Acting ◽  
Thyroid Microsomal Antibodies

ABSTRACT Crude IgG of sera from 3 patients with Graves' disease, which contained LATS-activity and/or thyroid antibodies, was fractionated by isoelectric focusing in a pH-range between 6.0 to 10.0. LATS-activity was found in IgG-subfractions from pH 7.5 to 9.5, thyroglobulin antibodies and thyroid microsomal antibodies from pH 6.0 to 10.0. It was not possible to separate LATS-activity from the thyroid antibodies by this technique. The results indicate that LATS and the thyroid antibodies are heterogeneous and of polyclonal origin.

Thyroid antibodies in blood donors: prevalence and clinical significance

1984 ◽  
Vol 105 (3) ◽  
pp. 324-329 ◽  
Author(s):  
T. Bjøro ◽  
P. I. Gaarder ◽  
E. B. Smeland ◽  
L. Kornstad
Keyword(s):  
Thyroid Function ◽  
Blood Donors ◽  
Thyroid Function Test ◽  
Thyroid Antibodies ◽  
Nodular Goitre ◽  
Thyroglobulin Antibodies ◽  
Function Test ◽  
Toxic Nodular Goitre ◽  
Thyroid Microsomal Antibodies

Abstract. In sera from 1643 randomly selected blood donors examined in 1979 thyroglobulin antibodies (TGA) were found in 3.4% and thyroid microsomal antibodies (TMA) in 7.0%. TMA, but not TGA, showed significant sex and age relationships. Eighty-two donors with TMA titres ≥ 1600 and/or TGA titres ≥ 128 were available for a follow-up study in 1982. In 69 of these with TMA there was a significantly increased incidence of pathological thyroid function-test values (T4, T3 and TSH) as compared to age- and sexmatched donors without thyroid antibodies. In this group 9 individuals had overt and three latent hypothyroidism, two individuals were found with symptomless autoimmune thyroiditis and one with a non-toxic nodular goitre. Only 2 of these had been diagnosed before 1982, and none recognized before 1979. In addition, the group contained 9 individuals who had been treated because of hyperthyroidism, all except one before 1979. The likelihood of detecting a previously unrecognized hypothyroidism increased with increasing TMA titre. In contrast, the 24 donors with TGA did not show an increased frequency of pathological thyroid function-test- values in 1982.

SERUM THYROTROPHIN AND CIRCULATING THYROGLOBULIN AND THYROID MICROSOMAL ANTIBODIES IN A FINNISH POPULATION

1979 ◽  
Vol 90 (1) ◽  
pp. 33-42 ◽  
Author(s):  
A. Gordin ◽  
J. Maatela ◽  
A. Miettinen ◽  
Tuula Helenius ◽  
B.-A. Lamberg
Keyword(s):  
Adult Population ◽  
Control Level ◽  
Thyroid Antibodies ◽  
Finnish Population ◽  
Thyroglobulin Antibodies ◽  
History Of ◽  
The Mean ◽  
Thyroid Microsomal Antibodies ◽  
Serum Tsh ◽  
Tsh Levels

ABSTRACT Circulating thyroglobulin antibodies (TgA) were detected in 7.9 % and thyroid microsomal antibodies (MsA) in 25.8 % of a sample of 507 subjects in the adult population of southern Finland when titres as low as 1/5 for TgA and 1/10 for MsA were included. Both antibodies were measured by passive haemagglutination techniques. The prevalence of high titres was 0.6 % for TgA (≥ 1/25 000) and 4.2% for MsA (≥ 1/100 000). Eightyeight per cent of the TgA positive subjects also had MsA. The MsA titres were usually markedly higher than those of TgA in the same subjects. Serum thyrotrophin (TSH), cholesterol and triglycerides were measured in 123 euthyroid subjects with thyroid antibodies and no history of thyroid diseases, and in controls matched for age and sex. Serum TSH was elevated in 31 % of the TgA positive subjects and the mean TSH concentration was significantly above that of the controls. All 3 subjects with high TgA titres also had elevated TSH levels. Serum TSH concentration was increased also in 25 % of those with MsA and the mean TSH value was significantly above the control level. High MsA titres were found in 19 subjects and the serum TSH was elevated in 10 of them (53%). The prevalence of symptomless autoimmune thyroiditis (SAT) was 2 and 5 %, of the population when high titres of either TgA or MsA, respectively, and elevation of serum TSH was used as criteria. This fits with our previous observations. There was no difference in serum cholesterol or triglycerides in the TgA or MsA positive subjects or in the corresponding matched controls.

Isolation of Antithrombin III from Normal and α1-Antitrypsin-Deficient Human Plasma

1977 ◽  
Vol 38 (02) ◽  
pp. 0475-0485 ◽  
Author(s):  
Anna D. Borsodi ◽  
Ralph A. Bradshaw
Keyword(s):  
Enzymatic Activity ◽  
Isoelectric Focusing ◽  
Antithrombin Iii ◽  
Factor Xa ◽  
Antithrombin Activity ◽  
Bovine Trypsin ◽  
Normal Plasma ◽  
Antitrypsin Deficiency ◽  
Simplified Procedure ◽  
Stimulation Of

SummaryThe plasma of individuals, hetero- or homozygous for α1-antitrypsin deficiency, contains greatly decreased amounts of antithrombin activity as assayed against factor Xa. However, heparin stimulation of the residual antithrombin activity is observed, which is comparable to that of normal plasma. Antithrombins isolated from both normal and α1-antitrypsin deficient plasma by a simplified procedure are indistinguishable in both properties and yields. The microheterogeneity observed on isoelectric focusing of both preparations can be eliminated by treatment with neuraminidase. Neither purified human antithrombin nor α1-antitrypsin, when assayed against bovine trypsin, is stimulated by heparin. These results clearly establish the unique natures of antithrombin and α1-antitrypsin and show that about 75% of the antithrombin activity measured in normal plasma is due to α1-antitrypsin. Estimates of anti thrombin III activity in normal plasma by assays dependent on enzymatic activity can probably be obtained only in the presence of heparin.

Hashimoto encephalopathy

2013 ◽  
Vol 154 (33) ◽  
pp. 1312-1316 ◽  
Author(s):  
Gábor Pocsay ◽  
Andrea Gazdag ◽  
József Engelhardt ◽  
István Szaniszló ◽  
Zoltán Szolnoki ◽  
...  
Keyword(s):  
Psychiatric Symptoms ◽  
Immunosuppressive Treatment ◽  
Unknown Origin ◽  
Corticosteroid Treatment ◽  
Thyroid Peroxidase ◽  
Thyroid Autoantibodies ◽  
Thyroglobulin Antibodies ◽  
Hashimoto Encephalopathy ◽  
Generalized Seizures

The authors present a case report and review the literature on Hashimoto encephalopathy. The onset of the disease may be marked by focal and then progressively generalized seizures or other neurological symptoms, but a cognitive decline or various psychiatric symptoms may also emerge. High levels of anti-thyroid peroxidase antibodies and/or anti-thyroglobulin antibodies are present in the serum. Corticosteroid treatment usually results in an improvement of symptoms. The syndrome is frequently overlooked and, therefore, the authors strongly recommend testing serum thyroid autoantibodies in cases with encephalopathy of unknown origin independently on the presence of thyroid disease in the patient or family history. The importance of long-term immunosuppressive treatment should also be stressed. Orv. Hetil., 2013, 154, 1312–1316.

Isoelectric focusing of insulin, 125I-insulin and the 125I-insulin-antibody complex

1979 ◽  
Vol 44 (6) ◽  
pp. 1828-1834
Author(s):  
Asja Šiševa ◽  
Jiřina Slaninová ◽  
Tomislav Barth ◽  
Stephan P. Ditzov ◽  
Luben M. Sirakov
Keyword(s):  
Isoelectric Focusing ◽  
Polyacrylamide Gel ◽  
The Other ◽  
Insulin Antibody ◽  
Isoelectric Points ◽  
Antibody Complex ◽  
And Storage ◽  
Acid Region ◽  
Three Components

Isoelectric focusing on polyacrylamide gel columns of three native crystalline commercial preparations of insulin and 125I-labelled insulin was carried out. All the compounds studied contained three components of different isoelectric points. The largest fraction, having pI 5.60 ± 0.05, was common to all preparations. The other two fractions were situated in the acid region of pH between pI 4.5 and 5.2. The presence of these fractions is explained by the contamination of crystalline insulins by proinsulin and by the formation of des-amido derivatives during the dissolving and storage of insulin samples, and, in case of labelled insulin, also by the presence of heavily iodinated insulin and contaminating components. The isoelectric focusing of the complex 125I-insulin-antibody showed a peak of radioactivity having pI 6.15 ± 0.05.

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