Downregulation of the pituitary-ovarian axis by different application forms of GnRH-analogues

ABSTRACT The present study has characterized the gonadotrophin-releasing hormone (GnRH) receptor in immortalized αT3-1 pituitary gonadotroph cells. GnRH and GnRH analogues produced both a dose- and time-dependent increase in total inositol phosphate (IP) accumulation. The rank order of potency of these analogues was the same as that obtained in parallel receptor-binding studies in αT3-1 cells. These responses were abolished following pretreatment with a GnRH antagonist. The use of a specific inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) assay demonstrated a rapid but short-lived rise in Ins(1,4,5)P3 production. Intracellular calcium ([Ca2+]i) was subsequently measured in αT3-1 cells using dual wavelength fluorescence microscopy combined with dynamic video imaging. GnRH produced a biphasic rise in [Ca2+]i. The initial calcium transient was complete within seconds while the smaller secondary plateau phase lasted several minutes. G-protein involvement in the IP response to GnRH in αT3-1 cells was investigated using sodium fluoride (NaF) and pertussis toxin (PTx) which activate and inactivate G-proteins respectively. Like GnRH, NaF produced a dose- and time-dependent increase in IP accumulation. Activation of phospholipase C in these cells by either GnRH or NaF was PTx-insensitive, suggesting that the G-protein involved was neither Gi nor Go but more probably Gq. Immunoblot analysis of αT3-1 cell membranes using antisera raised against the predicted C-terminal decapeptide of the α subunit of Gq demonstrated the presence of Gq in αT3-1 cells. Collectively these results show that the GnRH receptors expressed in αT3-1 cells are coupled to the phosphatidylinositol second messenger pathway via a specific G-protein. αT3-1 therefore represents a convenient model in which to study GnRH-related second messenger pathways. Journal of Endocrinology (1993) 136, 51–58

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GnRH-analogues and oral contraceptives for fertility preservation in women during chemotherapy

Human Reproduction Update ◽

10.1093/humupd/dmn022 ◽

2008 ◽

Vol 14 (6) ◽

pp. 543-552 ◽

Cited By ~ 130

Author(s):

Z. Blumenfeld ◽

M. von Wolff

Keyword(s):

Fertility Preservation ◽

Oral Contraceptives ◽

Gnrh Analogues

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Pengembangan Aplikasi SANTRI PTSL Untuk Percepatan Pengumpulan Data Yuridis di Kantor Pertanahan Kabupaten Kampar

Tunas Agraria ◽

10.31292/jta.v4i1.137 ◽

2021 ◽

Vol 4 (1) ◽

pp. 95-112

Author(s):

Mhd Teguh Saputra ◽

I Gusti Nyoman Guntur ◽

Slamet Wiyono

Keyword(s):

Data Collection ◽

Human Resources ◽

Qualitative Methods ◽

Task Force ◽

Land Registration ◽

Descriptive Approach ◽

Feasibility Test ◽

Application Forms

The purpose of this research is to find out how the SANTRI PTSL application can accelerate the juridical data collection and the feasibility of developing the SANTRI PTSL application. Using qualitative methods with a descriptive approach and data collection techniques by means of observation, interviews and questionnaires. SANTRI PTSL is an application that helps the work of the juridical task force. The output of the SANTRI PTSL application is a form of application for land registration that has been filled in automatically following the data submitted by the community to the officers. After training and simulating the use of the SANTRI PTSL application for juridical data collection officers, it is seen that this application is able to accelerate the collection and processing of juridical data, and in terms of the feasibility of developing this application it is also suitable for use in the Kampar Land Office after the feasibility test and simplification of the application forms according to the format used by the Land Office. By using the SANTRI PTSL application, the Kampar Land Office can optimize existing human resources and save time in completing PTSL activities. Inovasi pertanahan guna mendukung percepatan kinerja dan pelayanan pertanahan terus diupayakan. Penelitian ini bertujuan untuk mengetahui bagaimana aplikasi SANTRI PTSL dalam mempercepat pengumpulan data yuridis dan kelayakan pengembangan aplikasi SANTRI PTSL. Metode penelitian dilakukan secara kualitatif deskriptif, pengumpulan data dengan cara observasi dan wawancara.Hasil kajian menunjukkan bahwa aplikasi SANTRI PTSL yang diimplementasikan dapat mengoptimalkan SDM yang ada dan menghemat waktu dalam penyelesaian kegiatan PTSL. Aplikasi ini layak digunakan setelah dilakukanya pengembangan dan penyederhanaan blanko permohonan disesuaikan dengan standar yang ada pada Kantor Pertanahan Kabupaten Kampar. Aplikasi ini juga mampu mengintegrasikan data fisik dan data yuridis untuk mempermudah pengadministrasian pertanahan pada Kantor Pertanahan Kabupaten Kampar.Kata kunci: Pendaftaran Tanah, Inovasi, SANTRI PTSL

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Dienogest versus gonadotropin-releasing hormone analogue for the clinical treatment of endometriosis: a systematic review and meta-analysis

International Journal of Reproduction Contraception Obstetrics and Gynecology ◽

10.18203/2320-1770.ijrcog20174013 ◽

2017 ◽

Vol 6 (9) ◽

pp. 3712

Author(s):

Sofia Andrade De Oliveira ◽

Bárbara Souza Melo ◽

Marina Fernandes Pereira

Keyword(s):

Systematic Review ◽

Pelvic Pain ◽

Meta Analysis ◽

Uterine Cavity ◽

Lower Abdominal Pain ◽

Gnrh Analogue ◽

Hot Flushes ◽

Gnrh Analogues ◽

Great Loss ◽

Pouch Of Douglas

Endometriosis is a chronic inflammatory disease, defined by the presence of endometrial tissue outside the uterine cavity. It causes symptoms such as dysmenorrhea, chronic pelvic pain, dyspareunia, infertility, with great loss of quality of life for the patient. The objective of the study was to compare, through a meta-analysis, GnRH analogues, which are considered clinical first line treatment for endometriosis, versus dienogest, a selective oral progestin in the treatment of endometriosis. A systematic review was conducted to select the studies. In total, 31 articles were found. Four studies met criteria, the following variables were analyzed: pelvic pain, dyspareunia and induration of the Pouch of Douglas after treatment and it was evaluated the presence of side effects during treatment: hot flushes, headache and BMD loss. There was no difference between the dienogest group and GnRH analogue group when it was evaluated maintenance of lower abdominal pain, dyspareunia, induration of the Pouch of Douglas after treatment and hot flushes during treatment. Besides those results, the dienogest group had a lower incidence of headache and less BMD loss. The treatment of endometriosis continues to be a challenge, even with new treatment options such as new drugs (dienogest) and surgical procedures. This meta-analysis provides evidence of the absence of dienogest inferiority compared with GnRH analogues with less BMD loss and less headache incidence.

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