Quantitative Trait Loci Associated With Adventitious Shoot Formation in Tissue Culture and the Program of Shoot Development in Arabidopsis

Coconut water and rotten banana fruits are commonly found in traditional markets as organic wastes. One way to overcome the problems caused by these organic wastes is to convert these unuseful matter into an important and economically useful matter by using them as components of tissue culture media. One important commodity that is usually propagated by tissue culture is Phalaenopsis orchid type 229 (Phalaenopsis amabilis). Therefore, it would be more benefit to substitute the expensive chemicals with organic wastes such as coconut water and banana puree.In this experiment, addition of coconut water and banana pure to the minimum media containing commercial fertilizer red Polyhyponex, sucrose and commercial agar did not show any inhibition of Phalaenopsis orchid plantlet growth. This probably caused by sufficient macro and micro nutrients provided by those organic matter and Polyhyponexfertilizer. Moreover, addition of 100 mL/L of coconut water and 100 mg/L banana puree gave the optimum leaf and adventitious shoot formation. On the other hand, addition of 150 mL/L coconut water gave the optimum height and root formation. In this case,growing Phalaenopsis orchid plantlet should be done in 2 subculture period. The first subculture is to obtain maximum amount of leaf and shhot formation while the second subculture is to obtain optimum height and root formation.Key words : Waste, coconut water, banana puree, tissue culture, Phalaenopsis orchi

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STABILITY OF NUCLEAR DNA CONTENT DURING ADVENTITIOUS SHOOT FORMATION IN PINUS TAEDA L. TISSUE CULTURE

American Journal of Botany ◽

10.1002/j.1537-2197.1984.tb12513.x ◽

1984 ◽

Vol 71 (2) ◽

pp. 268-272 ◽

Cited By ~ 7

Author(s):

Michael H. Renfroe ◽

Graeme P. Berlyn

Keyword(s):

Tissue Culture ◽

Pinus Taeda ◽

Dna Content ◽

Nuclear Dna ◽

Nuclear Dna Content ◽

Adventitious Shoot ◽

Shoot Formation ◽

Adventitious Shoot Formation ◽

Pinus Taeda L

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Temporal and Spatial Expression Analysis of Shoot-Regeneration Regulatory Genes during the Adventitious Shoot Formation in Hypocotyl and Cotyledon Explants of Tomato (CV. Micro-Tom)

International Journal of Molecular Sciences ◽

10.3390/ijms21155309 ◽

2020 ◽

Vol 21 (15) ◽

pp. 5309 ◽

Cited By ~ 1

Author(s):

Myoung Hui Lee ◽

Jiyoung Lee ◽

Eun Yee Jie ◽

Seung Hee Choi ◽

Lingmin Jiang ◽

...

Keyword(s):

Gene Expression ◽

Tissue Culture ◽

Shoot Regeneration ◽

Expression Profiles ◽

Adventitious Shoot ◽

Shoot Formation ◽

Regulatory Genes ◽

Adventitious Shoot Formation ◽

Cotyledon Explants ◽

Temporal And Spatial

Enhancing the competence for plant regeneration in tissue culture studies is an important issue not only for efficient genetic transformation of commercial crops but also for the reproducibility of scientific reports. In this study, we investigated optimization of several tissue culture conditions including plant growth regulators, types and ages of explants, culture densities, and plant position in order to improve the competence of adventitious shoot formation of the tomato (Solanum lycopersicum cv. Micro-Tom). In addition, we examined the differential expression of D-type cyclin (CYCD3-1) and several shoot regeneration regulatory genes from hypocotyl and cotyledon explants of tomato during shoot organogenesis. A treatment of 1 mg L−1 Zeatin and 0.1 mg L−1 Indole-3-acetic acid (IAA) in Murashige and Skoog (MS) medium containing 3% sucrose was optimal for adventitious shoot formation from hypocotyl and cotyledon explants. The younger explants exhibited more shoot formation regardless of explant types. Additionally, those closest to the shoot apical meristem produced more shoots compared to the other regions in the hypocotyl and the cotyledon explants. Gene expression of CYCD3-1, SHOOT MERISTEMLESS (STM), and cytokinin dependent WUSCHEL (WUS) was significantly higher in younger explants than in older ones. Furthermore, an increase in CYCD3-1, STM, and WUS expression was evident at the distal part of hypocotyls and the proximal part of cotyledons compared to other regions. These differential gene expression profiles exhibited good agreement with the results of shoot formation obtained from diverse explants of tomato. These results suggest that temporal and spatial gene expression of shoot regeneration regulatory genes plays an important role in enhancing the competence and the reproducibility of adventitious shoot formation from tomato explants.

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