scholarly journals PEMANFAATAN LIMBAH BUAH PISANG DAN AIR KELAPA SEBAGAI BAHAN MEDIA KULTUR JARINGAN ANGGREK BULAN (Phalaenopsis amabilis) TIPE 229

2016 ◽  
Vol 11 (3) ◽  
pp. 373
Author(s):  
Ira Djajanegara
Keyword(s):  
Tissue Culture ◽  
Culture Media ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Organic Wastes ◽  
Coconut Water ◽  
Adventitious Shoot Formation ◽  
Tissue Culture Media ◽  
Optimum Height ◽  
Subculture Period

Coconut water and rotten banana fruits are commonly found in traditional markets as organic wastes. One way to overcome the problems caused by these organic wastes is to convert these unuseful matter into an important and economically useful matter by using them as components of tissue culture media. One important commodity that is usually propagated by tissue culture is Phalaenopsis orchid type 229 (Phalaenopsis amabilis). Therefore, it would be more benefit to substitute the expensive chemicals with organic wastes such as coconut water and banana puree.In this experiment, addition of coconut water and banana pure to the minimum media containing commercial fertilizer red Polyhyponex, sucrose and commercial agar did not show any inhibition of Phalaenopsis orchid plantlet growth. This probably caused by sufficient macro and micro nutrients provided by those organic matter and Polyhyponexfertilizer. Moreover, addition of 100 mL/L of coconut water and 100 mg/L banana puree gave the optimum leaf and adventitious shoot formation. On the other hand, addition of 150 mL/L coconut water gave the optimum height and root formation. In this case,growing Phalaenopsis orchid plantlet should be done in 2 subculture period. The first subculture is to obtain maximum amount of leaf and shhot formation while the second subculture is to obtain optimum height and root formation.Key words : Waste, coconut water, banana puree, tissue culture, Phalaenopsis orchi

scholarly journals In Vitro Regeneration through Direct Organogenesis from Protocorms of Oncidium tigrinum Llave & Lex. (Orchidaceae), an Endemic and Threatened Mexican Species

HortScience ◽  
2011 ◽  
Vol 46 (8) ◽  
pp. 1132-1135 ◽  
Author(s):  
Martín Mata-Rosas ◽  
Rosario Julieta Baltazar-García ◽  
Victor Manuel Chávez-Avila
Keyword(s):  
Culture Media ◽  
In Vitro Regeneration ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Direct Organogenesis ◽  
Naphthaleneacetic Acid ◽  
Average Height ◽  
Ms Medium ◽  
Adventitious Shoot Formation

A protocol for in vitro propagation from protocorms of Oncidium tigrinum Llave & Lex., a threatened species distributed in Mexico and highly appreciated as an ornamental, was developed. Two different explants, entire protocorms and longitudinal halves of protocorms, were used. In addition, the effect of two different culture media, Murashige and Skoog (MS) and modified Knudson (KCm), supplemented with N6-benzyladenine (BA) (0, 0.5, 1, 2, 3, and 5 mg·L−1) and/or α-naphthaleneacetic acid (NAA) at 0, 0.1, and 0.5 mg·L−1 was investigated. Adventitious shoot formation by direct organogenesis was obtained in all treatments; in some cases, the formation of protocorm-like bodies was induced. Shoot formation was greater for entire protocorms; the best treatment was MS medium containing at BA 1 to 2 mg·L−1 in combination with at NAA 0.1 mg·L−1. The average height of shoots was three times greater in MS medium than in KCm medium. Subculturing individual shoots in MS medium without plant growth regulators, but with 1 g·L−1 activated charcoal, allowed further development and rooting. An ex vitro survival rate of almost 100% was achieved. This study represents a comprehensive application for propagation, conservation, and sustainable use of this valuable natural resource.

scholarly journals Pengaruh Komposisi Media dalam Perbanyakan Protocorm Like Bodies, Pertumbuhan Planlet, dan Aklimatisasi Phalaenopsis amabilis

2015 ◽  
Vol 4 (3) ◽  
pp. 131 ◽  
Author(s):  
Erick Raynalta ◽  
Dewi Sukma
Keyword(s):  
Tissue Culture ◽  
Survival Rate ◽  
Culture Medium ◽  
Culture Media ◽  
Coconut Water ◽  
Basic Medium ◽  
Water Medium ◽  
Media Composition ◽  
Fresh Weight ◽  
Tissue Culture Media

<p>ABSTRACT</p><p>Phalaenopsis  amabilis  orchid  is one   of the most  popular  species  of orchids and  was inaugurated as one of the  national  flower ' Puspa  Pesona'. The purpose  of this study  was to determine the effects of   various  tissue culture   media composition on  Protocorm Like Bodies (PLBs)  multiplication,  growth  of plantlets,  and  acclimatization  of  Phalaenopsis  amabilis. Basic medium  used was  a half strengh of  Murashige and  Skoog (1/2MS)  and  Hyponex with additional  coconut  water,  benzyladeninepurine,  and  chitosan.  In  PLBs multiplication experiments ,   ½  MS  +  15%  coconut  water  medium produces  the  highest   percentage  of survival  rate.  ½  MS  +  15%  coconut water  medium  and  Hyponex  2  g  L-1+  15%  coconut water  +  2.5 ppm chitosan  medium  gave  the best  effect on  the fresh   weight  of  plantlets. The composition of  the culture medium   significantly affect   the fresh   weight and length of   root   on 8  WAP  (week after planting)   in the   acclimatization stage.</p><p>Key words: benzyladeninepurine, chitosan, coconut water, Hyponex,  tissue culture</p><p>ABSTRAK</p><p>Phalaenopsis amabilis  yang dikenal dengan  nama anggrek bulan merupakan salah satu jenisanggrek  yang  paling  populer  dan  dinobatkan  sebagai  salah satu  bunga  nasional  dengan  sebutan ‘Puspa Pesona’.  Tujuan penelitian ini untuk  mengetahui pengaruh berbagai komposisi media kultur jaringan dalam perbanyakan  Protocorm Like Bodies  (PLBs),  pertumbuhan  planlet, dan aklimatisasi Phalaenopsis amabilis. Media dasar yang digunakan adalah setengah konsentrasi media  Murashige dan  Skoog  (1/2MS)  dan  Hyponex dengan  tambahan  air  kelapa,  benziladeninpurin  (BAP),  dan kitosan. Pada percobaan perbanyakan PLBs, media ½ MS + 15% air kelapa menghasilkan persentase hidup tertinggi.  Media ½ MS + 15% air kelapa dan Hyponex  2 g L-1+ 15% AK + 2.5 ppm  kitosanmemberikan  respon  terbaik  terhadap  bobot planlet.  Komposisi  media  kultur  berpengaruh  nyata terhadap bobot segar dan panjang akar pada 8-MST (minggu setelah tanam) dalam tahap aklimatisasi.</p><p>Kata kunci: benzylaminopurin, kitosan, air kelapa, Hyponex, kultur jaringan</p>

scholarly journals Temporal and Spatial Expression Analysis of Shoot-Regeneration Regulatory Genes during the Adventitious Shoot Formation in Hypocotyl and Cotyledon Explants of Tomato (CV. Micro-Tom)

2020 ◽  
Vol 21 (15) ◽  
pp. 5309 ◽  
Author(s):  
Myoung Hui Lee ◽  
Jiyoung Lee ◽  
Eun Yee Jie ◽  
Seung Hee Choi ◽  
Lingmin Jiang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tissue Culture ◽  
Shoot Regeneration ◽  
Expression Profiles ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Regulatory Genes ◽  
Adventitious Shoot Formation ◽  
Cotyledon Explants ◽  
Temporal And Spatial

Enhancing the competence for plant regeneration in tissue culture studies is an important issue not only for efficient genetic transformation of commercial crops but also for the reproducibility of scientific reports. In this study, we investigated optimization of several tissue culture conditions including plant growth regulators, types and ages of explants, culture densities, and plant position in order to improve the competence of adventitious shoot formation of the tomato (Solanum lycopersicum cv. Micro-Tom). In addition, we examined the differential expression of D-type cyclin (CYCD3-1) and several shoot regeneration regulatory genes from hypocotyl and cotyledon explants of tomato during shoot organogenesis. A treatment of 1 mg L−1 Zeatin and 0.1 mg L−1 Indole-3-acetic acid (IAA) in Murashige and Skoog (MS) medium containing 3% sucrose was optimal for adventitious shoot formation from hypocotyl and cotyledon explants. The younger explants exhibited more shoot formation regardless of explant types. Additionally, those closest to the shoot apical meristem produced more shoots compared to the other regions in the hypocotyl and the cotyledon explants. Gene expression of CYCD3-1, SHOOT MERISTEMLESS (STM), and cytokinin dependent WUSCHEL (WUS) was significantly higher in younger explants than in older ones. Furthermore, an increase in CYCD3-1, STM, and WUS expression was evident at the distal part of hypocotyls and the proximal part of cotyledons compared to other regions. These differential gene expression profiles exhibited good agreement with the results of shoot formation obtained from diverse explants of tomato. These results suggest that temporal and spatial gene expression of shoot regeneration regulatory genes plays an important role in enhancing the competence and the reproducibility of adventitious shoot formation from tomato explants.

scholarly journals In Vitro Regeneration from Longitudinal Sections of Seedlings of Beaucarnea purpusii Rose, an Endemic and Endangered Species

HortScience ◽  
2016 ◽  
Vol 51 (3) ◽  
pp. 279-284 ◽  
Author(s):  
María del Carmen Vadillo-Pro ◽  
Luis Hernández-Sandoval ◽  
Guadalupe Malda-Barrera ◽  
María Luisa Osorio-Rosales ◽  
Martín Mata-Rosas
Keyword(s):  
Liquid Culture ◽  
Culture Media ◽  
In Vitro Regeneration ◽  
Sustainable Use ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Direct Organogenesis ◽  
Adventitious Shoot Formation ◽  
Efficient Alternative

The present study establishes an efficient protocol for in vitro propagation from longitudinal sections of seedlings of Beaucarnea purpusii, a threatened and highly appreciated ornamental species. The effect of three cytokinins: N6-benzyladenine (BA), kinetin (Kin), and thidiazuron, 1-phenyl-3-(1,2,3-thiadiazol-5-yl)urea (TDZ), in semisolid media and three different concentrations, as well as the effect of BA and TDZ pulses at higher concentrations in liquid culture medium, were investigated. Adventitious shoot formation by direct organogenesis was observed from all treatments. Additionally, adventitious shoot formation was recorded from the leaves of the new shoots; this particular response was exclusive to treatments supplemented with TDZ. In the experiment using semisolid culture media, the highest means of shoots per explant were obtained from treatments containing TDZ, particularly at a concentration of 0.45 μm (25.8 shoots per explant). For the pulses experiment, the liquid culture media supplemented with TDZ at 22.35 µm for 24 hours and 136.21 µm for 96 hours, induced a mean of 3.9 and 3.3 shoots per explant, respectively. Subculturing individual shoots on MS and half-strength MS (1/2MS) media, both supplemented with activated charcoal at 1 g·L−1, induced rooting in 85% to 95% of shoots. A survival rate of 100% under greenhouse conditions was achieved. The results of this study provide an efficient alternative for mass propagation of B. purpusii and may also contribute to the conservation and sustainable use of this valuable natural resource.

scholarly journals In vitro studies on Dendrobium fimbriatum Hk.F – An endangered orchid

2019 ◽  
pp. 100-103
Author(s):  
T. Ramesh ◽  
P. Renganathan ◽  
M. Prabhakaran
Keyword(s):  
Culture Media ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Direct Organogenesis ◽  
Naphthalene Acetic Acid ◽  
Average Height ◽  
Ms Medium ◽  
Adventitious Shoot Formation ◽  
Dendrobium Fimbriatum

A protocol for in vitro propagation from protocorms of Dendrobium fimbriatum a distributed in all over world and highly appreciated as an ornamental, was developed. Two different explants, entire protocorms and longitudinal halves of protocorms, were used. In addition, the effect of two different culture media, Murashige and Skoog (MS) and modified Knudson (KCm), supplemented with N6- benzyladenine (BA) (0, 0.5, 1, 2, 3, and 5 mg_L–1) and/or a-naphthalene acetic acid (NAA) at 0, 0.1, and 0.5 mg_L–1 was investigated. Adventitious shoot formation by direct organogenesis was obtained in all treatments; in some cases, the formation of protocorm like bodies was induced. Shoot formation was greater for entire protocorms; the best treatment was MS medium containing at BA 1 to 2 mg_L–1 in combination with at NAA 0.1 mg_L–1. The average height of shoots was three times greater in MS medium than in KC m medium. Sub culturing individual shoots in MS medium without plant growth regulators, but with 1 g_L–1 activated charcoal, allowed further development and rooting. An ex vitro survival rate of almost 100% was achieved. This study represents a comprehensive application for propagation, conservation, and sustainable use of this valuable natural resource.

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