scholarly journals Molecular Spectrum of Spontaneousde NovoMutations in Male and Female Germline Cells ofDrosophila melanogaster

Genetics ◽  
2008 ◽  
Vol 181 (3) ◽  
pp. 1035-1043 ◽  
Author(s):  
Yutaka Watanabe ◽  
Aya Takahashi ◽  
Masanobu Itoh ◽  
Toshiyuki Takano-Shimizu
Keyword(s):  
Molecular Spectrum ◽  
Male And Female ◽  
Germline Cells ◽  
Female Germline

scholarly journals A hobo Transgene That Encodes the P-Element Transposase in Drosophila melanogaster: Autoregulation and Cytotype Control of Transposase Activity

Genetics ◽  
2002 ◽  
Vol 161 (1) ◽  
pp. 195-204 ◽  
Author(s):  
Michael J Simmons ◽  
Kevin J Haley ◽  
Craig D Grimes ◽  
John D Raymond ◽  
Jarad B Niemi
Keyword(s):  
Drosophila Melanogaster ◽  
Gonadal Dysgenesis ◽  
P Element ◽  
Hsp70 Gene ◽  
Alternate Splicing ◽  
Male And Female ◽  
P Elements ◽  
Male Germline ◽  
Female Germline ◽  
Transposase Expression

Abstract Drosophila were genetically transformed with a hobo transgene that contains a terminally truncated but otherwise complete P element fused to the promoter from the Drosophila hsp70 gene. Insertions of this H(hsp/CP) transgene on either of the major autosomes produced the P transposase in both the male and female germlines, but not in the soma. Heat-shock treatments significantly increased transposase activity in the female germline; in the male germline, these treatments had little effect. The transposase activity of two insertions of the H(hsp/CP) transgene was not significantly greater than their separate activities, and one insertion of this transgene reduced the transposase activity of P(ry+, Δ2-3)99B, a stable P transgene, in the germline as well as in the soma. These observations suggest that, through alternate splicing, the H(hsp/CP) transgene produces a repressor that feeds back negatively to regulate transposase expression or function in both the somatic and germline tissues. The H(hsp/CP) transgenes are able to induce gonadal dysgenesis when the transposase they encode has P-element targets to attack. However, this ability and the ability to induce P-element excisions are repressed by the P cytotype, a chromosomal/cytoplasmic state that regulates P elements in the germline.

Isolation of Undifferentiated Female Germline Cells from Adult Drosophila Ovaries

2012 ◽  
Vol 22 (1) ◽  
Author(s):  
Robyn Su May Lim ◽  
Motomi Osato ◽  
Toshie Kai
Keyword(s):  
Germline Cells ◽  
Female Germline ◽  
Adult Drosophila

scholarly journals Drosophila male and female germline stem cell niches require the nuclear lamina protein Otefin

2016 ◽  
Vol 415 (1) ◽  
pp. 75-86 ◽  
Author(s):  
Lacy J. Barton ◽  
Kaylee E. Lovander ◽  
Belinda S. Pinto ◽  
Pamela K. Geyer
Keyword(s):  
Stem Cell ◽  
Nuclear Lamina ◽  
Germline Stem Cell ◽  
Male And Female ◽  
Female Germline ◽  
Stem Cell Niches

scholarly journals The virulent Wolbachia strain wMelPop increases the frequency of apoptosis in the female germline cells of Drosophila melanogaster

2012 ◽  
Vol 12 (Suppl 1) ◽  
pp. S15 ◽  
Author(s):  
Mariya V Zhukova ◽  
Elena Kiseleva
Keyword(s):  
Drosophila Melanogaster ◽  
Wolbachia Strain ◽  
Germline Cells ◽  
Female Germline

scholarly journals Male and female germline specific expression of an EGFP reporter gene in a unique strain of transgenic rats

2005 ◽  
Vol 284 (1) ◽  
pp. 171-183 ◽  
Author(s):  
Jennifer T. Cronkhite ◽  
Carola Norlander ◽  
Jenny K. Furth ◽  
Göran Levan ◽  
David L. Garbers ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Transgenic Rats ◽  
Specific Expression ◽  
Male And Female ◽  
Egfp Reporter Gene ◽  
Egfp Reporter ◽  
Unique Strain ◽  
Female Germline

scholarly journals Molecular genetics of the Drosophila melanogaster ovo locus, a gene required for sex determination of germline cells.

Genetics ◽  
1992 ◽  
Vol 130 (4) ◽  
pp. 791-803
Author(s):  
M D Garfinkel ◽  
A R Lohe ◽  
A P Mahowald
Keyword(s):  
Drosophila Melanogaster ◽  
Sex Determination ◽  
Genomic Dna ◽  
Transcription Unit ◽  
Complementation Group ◽  
P Element ◽  
Female Sterility ◽  
Germline Cells ◽  
Female Specific ◽  
Female Germline

Abstract The Drosophila melanogaster ovo gene is required for survival and differentiation of female germline cells, apparently playing a role in germline sex determination. We recovered 60 kb of genomic DNA from its genetic location at 4E1,2 on the X chromosome. A transcription unit coding for an apparently female-specific germline-dependent 5-kb poly(A)+ RNA size class is located substantially in a 7-kb region, within which three DNA-detectable lesions for mutations that inactivate the ovo function are located at two sites approximately 4 kb apart. The breakpoint of a deficiency that removes the neighboring lethal complementation group shavenbaby (svb) but leaves the ovo function intact maps approximately 5 kb to the molecular left of the leftmost ovo mutant site. A class of mutations that inactivates both the svb function and the ovo function affects genomic DNA between the two ovo sites. Sequences required for the two genetic functions are partly overlapping. In spite of this overlap, P element-mediated gene transfer of a 10-kb genomic DNA segment containing the 5-kb poly(A)+ RNA transcription unit rescues the female sterility phenotypes of ovo mutations, but not the svb lethality.

Isolation of Undifferentiated Female Germline Cells from Adult Drosophila Ovaries

2015 ◽  
Vol 34 (1) ◽  
Author(s):  
Robyn Su May Lim ◽  
Motomi Osato ◽  
Toshie Kai
Keyword(s):  
Germline Cells ◽  
Female Germline ◽  
Adult Drosophila

scholarly journals Binding of SU(VAR)3-9 Partially Depends on SETDB1 in the Chromosomes of Drosophila melanogaster

Cells ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 1030 ◽  
Author(s):  
Daniil A. Maksimov ◽  
Dmitry E. Koryakov
Keyword(s):  
Developmental Stages ◽  
Critical Role ◽  
Single Copy ◽  
Nurse Cells ◽  
Wild Type ◽  
Histone Methyltransferases ◽  
Adult Females ◽  
Germline Cells ◽  
Differentiation Stage ◽  
Female Germline

H3K9 methylation is known to play a critical role in gene silencing. This modification is established and maintained by several enzymes, but relationships between them are not fully understood. In the present study, we decipher the interplay between two Drosophila H3K9-specific histone methyltransferases, SU(VAR)3-9 and SETDB1. We asked whether SETDB1 is required for targeting of SU(VAR)3-9. Using DamID-seq, we obtained SU(VAR)3-9 binding profiles for the chromosomes from larval salivary glands and germline cells from adult females, and compared profiles between the wild type and SETDB1-mutant backgrounds. Our analyses indicate that the vast majority of single copy genes in euchromatin are targeted by SU(VAR)3-9 only in the presence of SETDB1, whereas SU(VAR)3-9 binding at repeated sequences in heterochromatin is largely SETDB1-independent. Interestingly, piRNA clusters 42AB and 38C in salivary gland chromosomes bind SU(VAR)3-9 regardless of SETDB1, whereas binding to the same regions in the germline cells is SETDB1-dependent. In addition, we compared SU(VAR)3-9 profiles in female germline cells at different developmental stages (germarium cells in juvenile ovaries and mature nurse cells). It turned out that SU(VAR)3-9 binding is influenced both by the presence of SETDB1, as well as by the differentiation stage.

The Balbiani Body in the Female Germline Cells of an Earwig,Opisthocosmia silvestris

2009 ◽  
Vol 26 (11) ◽  
pp. 754-757 ◽  
Author(s):  
Waclaw Tworzydlo ◽  
Malgorzata Kloc ◽  
Szczepan M. Bilinski
Keyword(s):  
Balbiani Body ◽  
Germline Cells ◽  
Female Germline
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