Delayed Neuronal Death by Transient Ligations of Bilateral Carotid Arteries in SHRSR, SHRSP and WKY

In addition to their role in signaling, Ca2+ ions in the endoplasmic reticulum also regulate important steps in protein processing and trafficking that are critical for normal cell function. Chronic depletion of Ca2+ in the endoplasmic reticulum has been shown to lead to cell degeneration and has been proposed as a mechanism underlying delayed neuronal death following ischemic insults to the CNS. Experiments here have assessed the relative content of ryanodine receptor-gated stores in CA1 neurons by measuring cytoplasmic Ca2+ increases induced by caffeine. These measurements were performed on CA1 neurons, in slice, from normal gerbils, and compared with responses from this same population of neurons 54–60 h after animals had undergone a standard ischemic insult: 5-min bilateral occlusion of the carotid arteries. The mean amplitude of responses in the postischemic population were less than one-third of those in control or sham-operated animals, and 35% of the neurons from postischemic animals showed very small responses that were ∼10% of the control population mean. Refilling of these stores after caffeine challenges was also impaired in postischemic neurons. These observations are consistent with our earlier finding that voltage-gated influx is sharply reduced in postischemic in CA1 neurons and the hypothesis that the resulting depletion in endosomal Ca2+ is an important cause of delayed neuronal death.

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Induction of acute and delayed neuronal death using caged calcium ionophore in neuron-glia mixed-cultures

Neuroscience Research ◽

10.1016/s0168-0102(00)81087-1 ◽

2000 ◽

Vol 38 ◽

pp. S40

Author(s):

H Sato

Keyword(s):

Neuronal Death ◽

Calcium Ionophore ◽

Mixed Cultures ◽

Delayed Neuronal Death ◽

Caged Calcium

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Accumulation of apolipoprotein E and β-amyloid-like protein in a trace of the hippocampal CA1 pyramidal cell layer after ischaemic delayed neuronal death

Neuroreport ◽

10.1097/00001756-199611250-00054 ◽

1996 ◽

Vol 7 (18) ◽

pp. 3063-3068 ◽

Cited By ~ 25

Author(s):

Hirohisa Ishimaru ◽

Koichi Ishikawa ◽

Seiichi Haga ◽

Mikio Shoji ◽

Yoshihide One ◽

...

Keyword(s):

Apolipoprotein E ◽

Pyramidal Cell ◽

Neuronal Death ◽

Cell Layer ◽

Delayed Neuronal Death ◽

Pyramidal Cell Layer ◽

Hippocampal Ca1 ◽

Β Amyloid

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A Comparative Study of Carotid Magnetic Resonance Plaque Imaging in Predicting the Early Progression of Acute Anterior Circulation Mild Stroke

Journal of Biomaterials and Tissue Engineering ◽

10.1166/jbt.2021.2664 ◽

2021 ◽

Vol 11 (6) ◽

pp. 1113-1119

Author(s):

Bo Wei ◽

Yiqin Xu ◽

Xiaohong Gui ◽

Chenglong Wu ◽

Liping Wang

Keyword(s):

Carotid Arteries ◽

Plaque Imaging ◽

Plaque Composition ◽

Anterior Circulation ◽

Progressive Group ◽

Bilateral Carotid ◽

Early Progression ◽

Before And After ◽

3.0 T ◽

Mild Stroke

To analyze the biological properties of carotid lumen stenosis and plaque before and after bifurcation of the bilateral carotid arteries in patients with acute anterior circulation mild stroke (AACMS) by 3.0 T high-resolution magnetic resonance imaging (HR-MRI) with the aim to clarify the predictive effect of 3.0 T HR-MRI on early progression and poor prognosis in patients with AACMS. Random division of 96 patients with AACMS into a stroke progression group and a non-progression group. The bilateral cervical vessels of the patients were detected by HR-MRI. The degree of carotid artery stenosis before and after bilateral carotid bifurcation was evaluated using a vascular plaque imaging diagnostic system. There were significant differences in the maximum and average wall standardization index, maximum wall thickness, maximum wall area, plaque composition, proportion of plaque fibrous cap rupture, and proportion of VI complex plaques in the progressive group. There was no significant difference in the related indexes of bilateral vessels in the non-progressive group (P >0.05). There are significant differences in MRI morphological characteristics of bilateral carotid plaques in patients with AACMS progression. Through a comparative analysis of the plaque load and plaque composition of bilateral carotid arteries using 3.0 T HR-MRI in patients with early-stage AACMS, the type and stability of complex plaques can be identified, which serve as prognostic factors in predicting the early progression of stroke and guiding clinical treatment.

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