scholarly journals EPIDEMIOLOGIC STUDY ON THE REPRODUCTIVE LOSSES IN DAIRY CATTLE: OCCURRENCE OF NEOSPORA CANINUM, BRUCELLA ABORTUS, BOVINE HERPESVIRUS TYPE-1 AND LEPTOSPIRA SPP IN A PROPRIETY OF SÃO CARLOS CITY / Estudo epidemiológico sobre as perdas reprodutivas em ...

2013 ◽  
Vol 29 (3) ◽  
pp. 153 ◽  
Author(s):  
R. C. M. SANTANA ◽  
R. MASSA ◽  
L. F. ZAFALON ◽  
J. MEGID ◽  
H. LANGONI ◽  
...  
2012 ◽  
Vol 15 (3) ◽  
pp. 561-563 ◽  
Author(s):  
K. Rypuła ◽  
K. Płoneczka-Janeczko ◽  
J. Kita ◽  
A. Kumala ◽  
J.F. Żmudziński

Abstract The objective of this study was to estimate a herd-level seroprevalence of bovine herpesvirus type 1 (BHV-1) in herds with clinical symptoms of the respiratory tract. Eighty-three herds with suspected BHV-1 infection were selected and divided into two categories with respect to their size: small (n=27) and large herds (n=56). Samples were collected from calves, heifers and cows older than 24 months. Seroprevalence was determined using the gB ELISA test. The herd level seroprevalence was estimated as 53% (44/83) in the tested herds, 11.1% (3/27) in the small herds and 73.2% (41/56) in the large herds. Our study suggests that the current biosecurity measures still warrant improvement.


2017 ◽  
Vol 7 (4) ◽  
pp. 278-283 ◽  
Author(s):  
Dora Romero-Salas ◽  
Cosme Alvarado-Esquivel ◽  
Gladys Domínguez-Aguilar ◽  
Anabel Cruz-Romero ◽  
Nelly Ibarra-Priego ◽  
...  

2015 ◽  
Vol 9 (9) ◽  
pp. 598-603 ◽  
Author(s):  
Severo Carvalho Osmana ◽  
Nayara R Gonzaga Ludmila ◽  
Saldanha Albuquerque Aline ◽  
Cutrim Bezerra Danilo ◽  
Pinto Chaves Nancyleni

2011 ◽  
Vol 152 (3-4) ◽  
pp. 270-279 ◽  
Author(s):  
S.I. Chowdhury ◽  
M.C.S. Brum ◽  
C. Coats ◽  
A. Doster ◽  
Huiyong Wei ◽  
...  

2017 ◽  
Vol 38 (6) ◽  
pp. 3915
Author(s):  
Greice Japolla ◽  
Ana Flávia Batista Penido ◽  
Greyciele Rodrigues Almeida ◽  
Luiz Artur Mendes Bataus ◽  
Jair Pereira Cunha Junior ◽  
...  

The specificity of monoclonal antibodies (mAbs) to desired targets makes these molecules suitable for therapeutic and diagnostic uses against a wide range of pathogens. Phage display antibody libraries offer one method by which mAbs can be selected for, without the use of conventional hybridoma technology. In this work, phage display technology was used to construct, select and characterize a combinatorial single chain fragment variable (scFv) antibody library against bovine herpesvirus type 1 (BoHV-1) from the immune repertoire of chickens immunized with the virus. In silico analysis of the hypervariable domains of the antibody heavy chains revealed a high frequency of scFv fragments with low variability, suggesting that selection had probably been carried out and favored by a few im-munogenic viral antigens. The reactivity of the scFv fragments selected against BoHV-1 was demon-strated by Phage-ELISA. A significant increase in antibody reactivity to the target was observed after six rounds of library selection, showing its potential use as a molecule for BoHV-1 diagnosis. The strategy described here opens up a field for the use of phage display as a tool for selection of mono-clonal antibodies that could be used for theranostic applications against infectious and parasitic dis-eases of veterinary interest.


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