scholarly journals OPTIMIZATION OF CULTIVATION CONDITIONS ex vitro OF JUVENILE MATERIAL OF PEDUNCULATE OAK

2019 ◽  
Keyword(s):  
Pedunculate Oak ◽  
Ex Vitro ◽  
Cultivation Conditions ◽  
Juvenile Material

scholarly journals Anatomical and physiological modifications of micropropagated 'Caipira' banana plants under natural lighy

2009 ◽  
Vol 66 (3) ◽  
pp. 323-330 ◽  
Author(s):  
Frederico Henrique da Silva Costa ◽  
Moacir Pasqual ◽  
Jonny Everson Scherwinski Pereira ◽  
Evaristo Mauro de Castro
Keyword(s):  
Sucrose Concentration ◽  
In Vitro Rooting ◽  
Production Costs ◽  
Tropical Species ◽  
Natural Light ◽  
Artificial Light ◽  
Ex Vitro ◽  
Cultivation Conditions ◽  
Growth Environment

Research about the use of natural light associated to changes in sucrose levels demonstrated potential in promoting in vitro hardiness of tropical climate species, as well as reducing production costs. However, little is known about physiological and structural changes that happen in the process. This study evaluated the physiological and anatomic performance, and ex vitro survival of micropropagated banana plants in response to cultivation conditions, in the stage of in vitro rooting. Shoots of the 'Caipira' cultivar were cultivated in MS medium, supplemented with 1 mg L-1 NAA and 6 g L-1 agar, in which the following treatments were applied: two sucrose concentrations (15 g L-1 or 30 g L-1) and two cultivation conditions (Natural light - greenhouse and Artificial light - growth chamber). At the end of 45 days, the contents of chlorophyll a, b and total, the relative water content in the tissues, anatomic characteristics and the ex vitro survival were evaluated. Effects of growth environment and sucrose concentration were observed on micropropagated 'Caipira' banana anatomy, physiology and survival. In vitro rooting of the shoots under natural light in the medium containing 15 g L-1 or 30 g L-1 sucrose promoted major alteration in the increase of palisade and spongy parenchyma, as well as reducing leaf water loss and plant death. The results obtained in the present study confirm the potential of the use of natural light as a substitute for artificial light for micropropagation of tropical species.

scholarly journals Optimization of in vitro rooting and ex vitro adaptation conditions of Melissa officinalis L. microshoots during clonal micropropagation

2021 ◽  
Vol 937 (4) ◽  
pp. 042093
Author(s):  
O V Yakimova ◽  
N A Yegorova
Keyword(s):  
Culture Medium ◽  
Alcoholic Beverage ◽  
Raw Material ◽  
Ex Vitro ◽  
Melissa Officinalis ◽  
Cultivation Conditions ◽  
Lemon Balm ◽  
Food Industries ◽  
Clonal Micropropagation

Abstract Melissa officinalis L. is a perennial herbaceous essential and medicinal plant widely used in pharmacology, perfumery and cosmetics, as well as in alcoholic beverage and food industries. The low content of essential oil in lemon balm raw material determines the selection work aimed at creating high-oil cultivars. The use of clonal micropropagation method in vitro will increase the efficiency of this process and accelerate promising breeding samples multiplication. The aim of our research was to study the influence of cultivation conditions and cultivar on the M. officinalis in vitro rhizogenesis and ex vitro adaptation. It was found that the maximum frequency of shoot rooting (up to 93.3%) in cultivars ‘Citronella’ and ‘Sobornaya’ was on MS culture medium supplemented with 0.5 mg/l NAA. The number of roots was 10.1 and 13.6 pcs. per shoot, respectively. The highest rates of root formation for the cv. ‘Crimchanka’ was found on a culture medium supplemented with 1.0 mg/l IAA (8.7 roots per shoot). The mixture of peat, sand and perlite (2:1:2) as a substrate provided up to 93% of adapted ex vitro lemon balm microplants. The presented studies were used to develop a technique for clonal micropropagation of M. officinalis.

Patrones de crecimiento de Cinchona officinalis in vitro y ex vitro; respuestas de plántulas micropropagadas y de semillas.

2017 ◽  
Vol 35 (1-2) ◽  
pp. 73-82
Author(s):  
Carlos Iván Espinosa ◽  
Gabriel Ríos
Keyword(s):  
Ex Vitro

El uso de herramientas biotecnológicas como la micropropagación se constituye en una alternativa de reproducción de especies amenazadas y con tamaños poblacionales reducidos. Sin embargo, uno de los problemas críticos en el uso de la micropropagación como herramienta de reproducción es la calidad de las plántulas resultantes en cuanto a su crecimiento y vigor. En el presente trabajo se evalua los efectos de la micropropagación sobre los patrones de crecimiento y sobrevivencia de plán­tulas in vitro de Cinchona officinalis L., una especie que ha sido fuertemente impactada por procesos de tala dentro de bosques naturales durante la época de la colonia. Se realizó un monitoreo de un total de 120 plántulas in vitro y 1988 plántulas ex vitro por 8 meses a partir del último repique. Adi­cionalmente, en cada plántula se contabilizó la cantidad de brotes axilares. Los resultados obtenidos mostraron un efecto remanente de los procesos de micropropagación, los cuales inicialmente inciden en la cantidad de brotes de las plántulas y en el crecimiento; sin embargo, este efecto no influye de forma negativa en la sobrevivencia de las plántulas durante la fase ex vitro

Konkurrenz zwischen Stieleiche und Buche auf Lothar-Sturmflächen | Competition between pedunculate oak and European beech on Lothar windthrow areas

2009 ◽  
Vol 160 (5) ◽  
pp. 114-123 ◽  
Author(s):  
Daniel Otto ◽  
Sven Wagner ◽  
Peter Brang
Keyword(s):  
Large Scale ◽  
Early Stage ◽  
Tree Height ◽  
European Beech ◽  
Threshold Value ◽  
Test Methods ◽  
Permanent Plots ◽  
Pedunculate Oak ◽  
Competitive Pressure ◽  
Competition Index

The competitive pressure of naturally regenerated European beech (Fagus sylvatica) saplings on planted pedunculate oak (Quercus robur) was investigated on two 1.8 ha permanent plots near Habsburg and Murten (Switzerland). The plots were established with the aim to test methods of artificial oak regeneration after large-scale windthrow. On both plots, 80 oaks exposed to varying levels of competitive pressure from at most 10 neighbouring beech trees were selected. The height of each oak as well as stem and branch diameters were measured. The competitive pressure was assessed using Schütz's competition index, which is based on relative tree height, crown overlap and distance from competing neighbours. Oak trees growing without or with only slight competition from beech were equally tall, while oaks exposed to moderate to strong competition were smaller. A threshold value for the competition index was found above which oak height decreased strongly. The stem and branch diameters of the oaks started to decrease even if the competition from beech was slight, and decreased much further with more competition. The oak stems started to become more slender even with only slight competition from beech. On the moderately acid beech sites studied here, beech grow taller faster than oak. Thus where beech is competing with oak and the aim is to maintain the oak, competitive pressure on the oak must be reduced at an early stage. The degree of the intervention should, however, take the individual competitive interaction into account, with more intervention if the competition is strong.

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