STUDY OF WUR10000125 POLYMORPHISM ASSOCIATION WITH MEAT, FATTENING AND REPRODUCTIVE TRAITS OF LANDRACE AND LARGE WHITE PIG BREEDS

Abstract. A scientific novelty is the study of the allele pool of domestic reproduction pigs, the determination of population genetic parameters by DNA markers, and the search for associations of genetic variants with economically significant traits. The aim of the work was to study the influence of SCD Y487830 polymorphism: g.2228 T > C to the IMF content and to search for associations of genotypes with economically significant traits in domestic reproduction pigs. The studies were conducted in 2019 on pigs reared in Selection and Hybrid Center LLC (Voronezh region). Genotyping were determined by the QuantStudio™ 5 Real-Time PCR System using the developed test system. The content of IMF (%), backfat thickness (mm), lean (%), live weight (kg) in 103 boars and 27 gilts Duroc pigs, 30 Large White boars was determined using an ExaGo ultrasound scanner. The main diet and animal welfare conditions were identical and corresponded to zoosanitary standards. Model equation for multivariate analysis of variance with interaction was used to evaluate the effect of genotype by SCD on productive qualities. The frequency of the T allele and TT genotype in the group of Duroc pigs was 0.419 and 12.7 %, respectively. Large White pig breeds were characterized by the frequency of the T allele – 0.686, TT genotype – 46.8 %. All Landrace pigs were carriers of the TT genotype. The superiority of the C allele carriers in the content of IMF was shown. The difference between Duroc boars with TT and CC genotypes was 0.42 % (P ≤ 0.05), and individuals with CC genotype exceeded TT genotype carriers in the backfat thickness of more than 3 mm (P ≤ 0.001). The results confirm the association of the SCD genotype with the content of intramuscular fat and the backfat thickness in the Duroc boars.

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Variants in BMP7 and BMP15 3’-UTRs Associated with Reproductive Traits in a Large White Pig Population

Animals ◽

10.3390/ani9110905 ◽

2019 ◽

Vol 9 (11) ◽

pp. 905 ◽

Cited By ~ 2

Author(s):

Yin ◽

Du ◽

Li ◽

Pan ◽

Wu ◽

...

Keyword(s):

Point Mutations ◽

Reproductive Traits ◽

Regulatory Elements ◽

Large White ◽

Morphogenetic Protein ◽

Pooled Dna ◽

Trait Locus ◽

Whole Genome Resequencing ◽

Weight Trait ◽

Large White Pig

Bone morphogenetic protein 7 (BMP7) and BMP15, which encode members of the BMP family, have been identified by whole-genome resequencing as breeding-related genes that overlap with a known quantitative trait locus for reproductive traits. In this study, we investigated the effects of variants at the BMP7 and BMP15 gene loci on sow reproductive traits. We isolated 669 and 1213 bp sequences of the 3’-untranslated region (3’-UTR) of the porcine BMP7 and BMP15 genes, respectively, and detected several RNA regulatory elements, such as miRNA response elements and AU-rich elements. Pooled DNA sequencing identified two novel point mutations (viz., BMP7 c.1569A>G and BMP15 c.2366G>A) in the 3’-UTR. Association analysis showed that the c.1569A>G polymorphism was associated with the litter weight trait in a Large White pig population. Furthermore, analysis of the combined genetic effects revealed that AA/GA and AG/GG were the favorable combined genotypes for the total number of piglets born (TNB) and the total number of piglets born alive (NBA), whereas. Together, our findings confirm that BMP7 and BMP15 are candidate genes for porcine reproductive performance.

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P5063 The effect of the IGF2 gene on pork and fat quality traits in two populations of the South African Landrace and Large White pig breeds

Journal of Animal Science ◽

10.2527/jas2016.94supplement4145b ◽

2016 ◽

Vol 94 (suppl_4) ◽

pp. 145-146

Author(s):

P. D. Soma

Keyword(s):

South African ◽

Quality Traits ◽

The South ◽

Large White ◽

Igf2 Gene ◽

Pig Breeds ◽

Fat Quality ◽

Two Populations ◽

Large White Pig

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Association and polymorphism study of seven candidate genes with reproductive traits in three pig breeds in Hungary.

Acta Biochimica Polonica ◽

10.18388/abp.2015_1188 ◽

2016 ◽

Vol 63 (2) ◽

Cited By ~ 3

Author(s):

Ágnes Hunyadi-Bagi ◽

Péter Balogh ◽

Krisztina Nagy ◽

Szilvia Kusza

Keyword(s):

Growth Rate ◽

Association Study ◽

Significant Influence ◽

Reproductive Traits ◽

Large White ◽

Pig Breeds ◽

Pcr Rflp ◽

Reproduction Traits ◽

Hardy Weinberg Equilibrium ◽

Selection For

Seven genes (BF, EGF, ESR, FSHB, H2AFZ, LEP and PRLP) were studied as candidate gene influencing eleven reproduction traits (interval between litters (IBL), percent of litter (PL), number of litters (NL), number of piglets born dead (NBD), number of piglet born alive (NBA), total number born (TNB), mean of born alive (MBA), mean of born dead (MBD), mean of born total (MBT), mean of piglets at 21 days of age (M21D) and growth rate (GR) in three pig breeds (Hungarian Large White (HLW), Duroc and Pietrain) by PCR-RFLP. Based on the observed vs. expected genotypes frequencies populations across loci were in Hardy-Weinberg equilibrium (P>0.05). In case HLW breed ESR and FSHβ genes were in disequilibrium. Association study suggested that only EGF gene showed significant influence on the trait NBA and TNB. The AA genotype are preferable for sows, associated with higher NBA and TNB. The longest IBL, and the highest NL is associated with AB and AA genotype of EGF gene. IBL is significantly shorter in case of pigs with AB and AA alleles than BB alleles of PRLP genes. Selection for these SNPs could improve the reproductivity in the studied breeds.

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