Evaluation of a Commercial Latex Particle Agglutination Test for Rapid Diagnosis of Haemophilus influenzae Type b Infection

PEDIATRICS ◽  
1982 ◽  
Vol 69 (4) ◽  
pp. 466-471
Author(s):  
Robert S. Daum ◽  
George R. Siber ◽  
Jill S. Kamon ◽  
Rebecca R. Russell
Keyword(s):  
Haemophilus Influenzae ◽  
Latex Particle ◽  
Rapid Diagnosis ◽  
Agglutination Test ◽  
Haemophilus Influenzae Type ◽  
Haemophilus Influenzae Type B ◽  
Type B ◽  
Urine Specimens ◽  
Particle Agglutination

The effectiveness of a commercially available latex particle agglutination test (Bactogen) in the diagnosis of invasive Haemophilus influenzae type b infection was evaluated. Bactogen correctly diagnosed all 27 patients with bacteriologically proven H influenzae type b infection (sensitivity 100%). Two of 39 patients with proven, non-H influenzae type b infections had false-postive tests (specificity 95%). One of 103 sera and 0 of 55 urine specimens from hospitalized adults contained detectable H influenzae type b antigen. Bactogen is a sensitive, specific, commercially available test for rapid diagnosis of H influenzae type b infection.

scholarly journals Detection of Haemophilus influenzae type b antigens in body fluids, using specific antibody-coated staphylococci

1977 ◽  
Vol 5 (1) ◽  
pp. 81-85
Author(s):  
M Suksanong ◽  
A S Dajani
Keyword(s):  
Cerebrospinal Fluid ◽  
Haemophilus Influenzae ◽  
Protein A ◽  
Body Fluids ◽  
Haemophilus Influenzae Type ◽  
Haemophilus Influenzae Type B ◽  
Bacterial Cells ◽  
Type B ◽  
Initiation Of Therapy ◽  
Urine Specimens

Protein A-rich staphylococci coated with Haemophilus influenzae type b antiserum agglutinate specifically with homologous bacterial cells or with cell-free supernatant fluids of cultures of the organism. Antibody-coated staphylococci were used to detect soluble antigens in body fluids of patients infected with H. influenzae type b. Cerebrospinal fluid from 36 cases of meningitis caused by this orgainsm showed positive coagglutination tests in 86% of patients prior to initiation of therapy. Antigens could be detected in 46% of sterile cerebrospinal fluid specimens obtained from the same cases 1 to 10 days after therapy. Soluble antigens were also detectable in sera (58%) and urine specimens (67%) of patients with H. influenzae type b septicemia, when such specimens were tested within 10 days of onset of illness. No antigen could be detected in body fluids beyond 10 days. The coagglutination test was positive in 57% of all body fluids examined; contercurrent immunoelectrophoresis (CCIE) was positive in only 27%. All specimens positive by CCIE were also positive by coagglutination. No false-positive reactions were noted by either test in body fluids from controls. The coagglutination test is simple, specific, and more sensitive than the CCIE method and could be a valuable tool for detecting antigens in body fluids of patients with various infections.

scholarly journals Rapid Diagnosis of Bacterial Pneumonia in Under-five Children by Latex Particle Agglutination Test in Urine

2016 ◽  
Vol 1 (1) ◽  
pp. 17-20
Author(s):  
Shahin Ara Begum ◽  
Aliya Rashid ◽  
Md. Abdur Rhman ◽  
Akhtaruzzman Chowdhury ◽  
Sufi HZ Rahman ◽  
...  
Keyword(s):  
Bacterial Pneumonia ◽  
Latex Particle ◽  
Agglutination Test ◽  
Haemophilus Influenzae Type ◽  
Control Group ◽  
X Rays ◽  
Type B ◽  
Clinical Criteria ◽  
Cell Counts ◽  
Particle Agglutination

Streptococcus pneumoniae and Haemophilus influenzae type b are the main causes of community-acquired bacterial pneumonia in developing countries including Bangladesh. This study was carried out to find a rapid diagnostic method for early detection of bacterial pneumonia. Latex Particle Agglutination Test (LPAT) was done in urine for quick detection of capsular antigens of S. pneumoniae and H. influenzae type b. Three hundred children were enrolled based on clinical criteria as defined by the WHO. Among them, 70 pneumonic children, who had total white blood cell counts around  20000/ mm3, Neutrophils ? 75% and consolidation on chest X-rays, were selected for LPAT in urine. Some 20 children of the same age group, who had chest diseases other than pneumonia, were selected as sick control group. Among 70 pneumonic cases, 36 (51.43%) were positive by LPAT in urine. Out of 36 LPAT-positive cases, 19 (52.7%) were positive for S. pneumoniae and 17 (47.3%) were positive for H. influenzae type b by specific anti-sera. The LPAT is thus appears as a quick and reliable test for the diagnosis of bacterial pneumonia cases caused by S. pneumoniae and H. influenzae type b.Bangladesh J Med Microbiol 2007; 01 (01):17-20 

Pneumococcal and Haemophilus influenzae Type b Antigen Detection in Children at Risk for Occult Bacteremia

PEDIATRICS ◽  
1987 ◽  
Vol 80 (1) ◽  
pp. 92-96 ◽  
Author(s):  
Lorry G. Rubin ◽  
Lillian Carmody
Keyword(s):  
Haemophilus Influenzae ◽  
Enzyme Immunoassay ◽  
Latex Agglutination ◽  
Haemophilus Influenzae Type ◽  
Haemophilus Influenzae Type B ◽  
Type B ◽  
Children At Risk ◽  
Pneumococcal Bacteremia ◽  
Occult Bacteremia ◽  
Urine Specimens

We prospectively evaluated pneumococcal and Haemophilus influenzae type b antigen detection in serum and urine of young (3 to 30 months of age) febrile (temperature ≥ 39°C) children at risk for occult bacteremia. Patients with septic shock, meningitis, or epiglottitis were excluded. Of 576 patients, 16 had pneumococcal bacteremia (final diagnoses: primary bacteremia, nine; otitis media, four; pneumonia, two; unknown, one), and five had H influenzae b bacteremia (final diagnoses: primary bacteremia, two; cellulitis, two; arthritis, one). Latex agglutination was positive in all five patients with H influenzae b bacteremia (positive in three of three urine specimens, three of four sera tested) but only one of 16 patients with pneumococcal bacteremia (positive in one of seven urine samples, zero of 13 sera tested). Both assays had specificities of greater than 95%. Nonspecific agglutination occurred in 7% of specimens tested. Enzyme immunoassay for pneumococcal antigen, although more sensitive than latex agglutination, failed to detect antigen in ten sera and three urine specimens from patients with pneumococcal bacteremia. Thus, neither latex agglutination nor enzyme immunoassay was sufficiently sensitive for detection of occult pneumococcal bacteremia. Latex agglutination for H influenzae b holds promise as a sensitive and specific test for rapid diagnosis of occult bacteremia due to H influenzae b.

Advantage of Latex Agglutination Over Countercurrent Immunoelectrophoresis in the Detection of Haemophilus influenzae Type b Antigen in Serum

PEDIATRICS ◽  
1981 ◽  
Vol 68 (6) ◽  
pp. 888-891
Author(s):  
David W. Scheifele ◽  
Joel I. Ward ◽  
George R. Siber
Keyword(s):  
Haemophilus Influenzae ◽  
Latex Particle ◽  
Capsular Polysaccharide ◽  
Latex Agglutination ◽  
Haemophilus Influenzae Type ◽  
Enzyme Linked Immunosorbent Assay ◽  
Haemophilus Influenzae Type B ◽  
Type B ◽  
Valuable Adjunct ◽  
Invasive Infections

Detection of bacterial antigens has proved to be a valuable adjunct to early diagnosis of certain invasive infections. In the case of Haemophilus influenzae type b infections, capsular polysaccharide antigen (PRP) has been detected by a variety of techniques including countercurrent immunoelectrophoresis (CIE),1-3 latex particle agglutination (LPA),3,4 radioimmunoassay,5 and enzyme-linked immunosorbent assay (ELISA).6,7 There have been few prospective comparisons3,7,8 by which to establish the sensitivities and idiosyncrasies of these techniques. This report compares the two simplest techniques, CIE and LPA, in children with H influenzae b infections, drawing attention to a shortcoming of CIE in those with nonmeningeal infections. METHODS

Sign in / Sign up

Export Citation Format

Share Document