scholarly journals Rapid Diagnosis of Bacterial Pneumonia in Under-five Children by Latex Particle Agglutination Test in Urine

2016 ◽  
Vol 1 (1) ◽  
pp. 17-20
Author(s):  
Shahin Ara Begum ◽  
Aliya Rashid ◽  
Md. Abdur Rhman ◽  
Akhtaruzzman Chowdhury ◽  
Sufi HZ Rahman ◽  
...  
Keyword(s):  
Bacterial Pneumonia ◽  
Latex Particle ◽  
Agglutination Test ◽  
Haemophilus Influenzae Type ◽  
Control Group ◽  
X Rays ◽  
Type B ◽  
Clinical Criteria ◽  
Cell Counts ◽  
Particle Agglutination

Streptococcus pneumoniae and Haemophilus influenzae type b are the main causes of community-acquired bacterial pneumonia in developing countries including Bangladesh. This study was carried out to find a rapid diagnostic method for early detection of bacterial pneumonia. Latex Particle Agglutination Test (LPAT) was done in urine for quick detection of capsular antigens of S. pneumoniae and H. influenzae type b. Three hundred children were enrolled based on clinical criteria as defined by the WHO. Among them, 70 pneumonic children, who had total white blood cell counts around  20000/ mm3, Neutrophils ? 75% and consolidation on chest X-rays, were selected for LPAT in urine. Some 20 children of the same age group, who had chest diseases other than pneumonia, were selected as sick control group. Among 70 pneumonic cases, 36 (51.43%) were positive by LPAT in urine. Out of 36 LPAT-positive cases, 19 (52.7%) were positive for S. pneumoniae and 17 (47.3%) were positive for H. influenzae type b by specific anti-sera. The LPAT is thus appears as a quick and reliable test for the diagnosis of bacterial pneumonia cases caused by S. pneumoniae and H. influenzae type b.Bangladesh J Med Microbiol 2007; 01 (01):17-20 

Evaluation of a Commercial Latex Particle Agglutination Test for Rapid Diagnosis of Haemophilus influenzae Type b Infection

PEDIATRICS ◽  
1982 ◽  
Vol 69 (4) ◽  
pp. 466-471
Author(s):  
Robert S. Daum ◽  
George R. Siber ◽  
Jill S. Kamon ◽  
Rebecca R. Russell
Keyword(s):  
Haemophilus Influenzae ◽  
Latex Particle ◽  
Rapid Diagnosis ◽  
Agglutination Test ◽  
Haemophilus Influenzae Type ◽  
Haemophilus Influenzae Type B ◽  
Type B ◽  
Urine Specimens ◽  
Particle Agglutination

The effectiveness of a commercially available latex particle agglutination test (Bactogen) in the diagnosis of invasive Haemophilus influenzae type b infection was evaluated. Bactogen correctly diagnosed all 27 patients with bacteriologically proven H influenzae type b infection (sensitivity 100%). Two of 39 patients with proven, non-H influenzae type b infections had false-postive tests (specificity 95%). One of 103 sera and 0 of 55 urine specimens from hospitalized adults contained detectable H influenzae type b antigen. Bactogen is a sensitive, specific, commercially available test for rapid diagnosis of H influenzae type b infection.

Counterimmunoelectrophoresis of Urine for Diagnosis of Bacterial Pneumonia in Pediatric Outpatients

PEDIATRICS ◽  
1983 ◽  
Vol 71 (5) ◽  
pp. 780-783
Author(s):  
Ronald B. Turner ◽  
Frederick G. Hayden ◽  
J. Owen Hendley
Keyword(s):  
Haemophilus Influenzae ◽  
Bacterial Pneumonia ◽  
Clinical Spectrum ◽  
Haemophilus Influenzae Type ◽  
Haemophilus Influenzae Type B ◽  
Type B ◽  
Older Children ◽  
Pediatric Outpatients ◽  
Etiologic Diagnosis

Thirty-eight pediatric outpatients with pneumonia were studied by counterimmunoelectrophoresis for the presence of Haemophilus influenzae type b or pneumococcal antigenuria. Of the 38 patients eight (21%) hadH influenzae type b antigenuria and two (5%) had pneumococcal antigenuria. H influenzae, type b antigenuria was detected more frequently in patients <2 years of age than in older children. Urine counterimmunoelectrophoriesis appears to be a useful tool for the etiologic diagnosis of bacterial pneumonia and should facilitate further studies of the epidemiology, pathogenesis, and clinical spectrum of this disease.

Letters to the Editor

PEDIATRICS ◽  
1980 ◽  
Vol 66 (1) ◽  
pp. 154-155
Author(s):  
Charles M. Ginsburg ◽  
John D. Nelson
Keyword(s):  
Staphylococcus Aureus ◽  
Haemophilus Influenzae ◽  
Bacterial Pneumonia ◽  
Hospitalized Patients ◽  
Haemophilus Influenzae Type ◽  
Haemophilus Influenzae Type B ◽  
Type B ◽  
Letters To The Editor ◽  
And Staphylococcus Aureus

We do not disagree with the recommendations of Drs Fischer, Bass, and Arthur for treating hospitalized patients with pneumonia. They might have mentioned, additionally, the possible utility of cefamandole as an alternative to a penicillinase-resistant penicillin plus chloramphenicol for hospitalized infants with presumed bacterial pneumonia. We are currently evaluating cefuroxime, which has a similar in vitro spectrum, and are finding it effective in patients with pneumonia due to Haemophilus influenzae type b, pneumococci and Staphylococcus aureus.

A Slide Latex-Particle Agglutination Test for Trichinosis

1962 ◽  
Vol 37 (2_ts) ◽  
pp. 227-231 ◽  
Author(s):  
Thelma F. Muraschi ◽  
Norman Bloomfield ◽  
Robert B. Newman
Keyword(s):  
Latex Particle ◽  
Agglutination Test ◽  
Particle Agglutination

scholarly journals Comparison of staphylococcal coagglutination, latex agglutination, and counterimmunoelectrophoresis for bacterial antigen detection

1979 ◽  
Vol 9 (1) ◽  
pp. 28-32
Author(s):  
M C Thirumoorthi ◽  
A S Dajani
Keyword(s):  
Protein A ◽  
Group B Streptococcus ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Haemophilus Influenzae Type ◽  
Bacterial Antigen ◽  
Haemophilus Influenzae Type B ◽  
Type B ◽  
Streptococcal Antigens ◽  
Group B

Soluble antigens of Haemophilus influenzae type b, Streptococcus pneumoniae, Neisseria meningitidis, and group B streptococcus were looked for in cerebrospinal fluid, serum, and urine by using the staphylococcal coagglutination test, latex agglutination test, and counterimmunoelectrophoresis. The staphylococcal coaggultination and latex agglutination tests were more sensitive than counterimmunoelectrophoresis in identifying antigens of H. influenzae type b, S. pneumoniae, and N. meningitidis. None of the three tests successfully detected group B streptococcal antigens in body fluids. Nonspecific reactions noted with the staphylococcal coagglutination test could be usually eliminated after premixing test specimens with soluble protein A.

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