scholarly journals The usage of nitrogen compounds by purple non-sulfur bacteria of the Rhodopseudomonas genus

2019 ◽  
Vol 10 (1) ◽  
pp. 83-86 ◽  
Author(s):  
О. V. Tarabas ◽  
S. О. Hnatush ◽  
О. М. Мoroz
Keyword(s):  
Sulfanilic Acid ◽  
Zinc Powder ◽  
Electron Donors ◽  
Nitrogen Compounds ◽  
Cultivation Medium ◽  
Nitrate Ions ◽  
Sulfur Bacteria ◽  
Ethylenediamine Dihydrochloride ◽  
Phototrophic Growth

In this article, we characterized the regularities of oxidation of nitrite ions by phototropic purple non-sulfur bacteria Rhodopseudomonas yavorovii IMV B-7620, which were isolated from the water of Yavorivske Lake (Lviv Region, Ukraine). The bacteria were cultivated anaerobically at the light intensity of 200 lux and aerobically without illumination for 13 days in the modified ATCC No. 1449 medium. The concentration of nitrite ions was determined turbidimetrically by the turbidity of the solution by method of diazotization of sulfanilic acid by the nitrite ions and the interaction of the formed salt with n-(l-naphtyl)ethylenediamine dihydrochloride. The concentration of nitrate ions was determined turbidimetrically by the turbidity of the solution by method of diazotization. Zinc powder was used as a reducing agent. Efficiency of oxidation of 0.7–5.6 mM nitrite ions as electron donors by these bacteria was 100–7%, on the 10-th day of cultivation. It was established that nitrate ions were accumulated in the medium as a result of oxidation of nitrite ions by bacteria. The largest biomass (1.6 g/L) bacteria accumulated on the thirteenth day of growth in a medium with 2.8 mM NO2–. We found that R. yavorovii can use nitrate ions and urea as the only source of nitrogen for phototrophic growth. At a concentration of 1.9 mM ammonium chloride, sodium nitrite and urea in the cultivation medium, the biomass of bacteria was 1.2, 0.8, 1.0 g/L, respectively. The ability of the studied microorganisms to oxidize nitrite ions and to use nitrate ions indicates the significant impact of purple non-sulfur bacteria on the redistribution of streams of nitrogen compounds in ecosystems and the essential role of these microorganisms in the nitrogen biogeochemical cycle.

Notizen: Formation of Erythroascorbic Acid and 2,3-Enediol Pentonic Acid from ʟ-Ascorbic Acid by Purple Sulfur Bacteria

1978 ◽  
Vol 33 (9-10) ◽  
pp. 789-792 ◽  
Author(s):  
Eckhard Bast ◽  
Friedhelm Marx ◽  
Konrad Pfeilsticker
Keyword(s):  
Ascorbic Acid ◽  
Slow Growth ◽  
Uv Spectra ◽  
Electron Donors ◽  
Chromatium Vinosum ◽  
Purple Sulfur Bacteria ◽  
Thiocapsa Roseopersicina ◽  
Sulfur Bacteria ◽  
Brown Colour ◽  
Phototrophic Growth

Abstract Phototrophic growth of Chromatium vinosum strain D on pyruvate or malate, and of Thiocapsa roseopersicina strain 6311 on malate or sulfide as sole electron donors was promotedby 5 mᴍ ʟ-ascorbate, while growth of T. roseopersicina on fructose was inhibited. Slow growth was obtained also with ascorbate alone. In ascorbate-containing cultures the medium developed a yellow to red-brown colour which showed an absorption maximum at 310 -320 nm. As colourless products of ʟ-ascorbic acid degradation by the two strains examined erythroascorbic acid and 2,3-enediol pentonic acid were found in cell-free culture filtrates by gas chromatographic analysis of the silylated residues and identified by their mass, IR and UV spectra.

scholarly journals The Effect of Boiling Time and the Type of Utensil Used on the Nitrite and Nitrate Contents in Carrots (Daucus carota L.)

2018 ◽  
Vol 1 (1) ◽  
pp. 18-27 ◽  
Author(s):  
Jansen Silalahi ◽  
Shena Keshia Aritonang ◽  
Muchlisyam
Keyword(s):  
Stainless Steel ◽  
Sulfanilic Acid ◽  
Zinc Powder ◽  
Nitrate Level ◽  
Daucus Carota L ◽  
Nitrite Level ◽  
Ethylenediamine Dihydrochloride ◽  
Nitrate And Nitrite ◽  
North Sumatra

Abstract.  The purpose of this study was to investigate the effect of boiling time using utensils made of different metal component on thenitrate and nitrite contentsin carrots. The carrots were obtained from Jaranguda Village, Karo Regency, North Sumatra. The utensil types used for boiling were made of stainless steel, what so called periuk and aluminum and boiling time were 5 minutes, 10 minutes and 15 minutes.The determination of nitrite was done by visible spectrophotometer using sulfanilic acid and N-(1-naphthyl) ethylenediamine dihydrochloride reagentsand absorbance was measured at a wavelength of 540 nm. The determinationof nitrate wascarried out by reducing the nitrate into nitrite using Zinc powder and diluted HCl then measured as nitrite. The nitritelevel was then convertedinto nitrate. The result showed that the utensil types and boiling time affected the levels of nitrate and nitrite in carrots. The nitrate and nitrite levels in fresh carrots was 32.14 mg/kg and 24.78 mg/kg respectively. After boiling for 5 minutes, the nitrate and nitrite levels decreased significantly. Further boiling of boiled carrots, the nitrite levelincreased in the aluminum utensil from 11.00 mg/kg to 20.15 mg/kg (83 %); in periuk from 9.18 mg/kg to 16.95 mg/kg (78%) andin stainless steelfrom 8.21 mg/kg to 11.75 mg/kg (43%). While the nitrate level decreased in aluminumutensil from 27.14 mg/kg to 21.08 mg/kg (22%); stainless steel from 16.40 mg/kg to 13.25 mg/kg (19%) and periuk from 20.30 mg/kg to 16.51 mg/kg (18%).  The results of this study indicated that the nitrite level increased, while nitrate level decreased with boiling time.The effect of utensil type used on boiling increased nitrite but decreased nitrate level in carrotsand these effects were found that the mostinfluential treatmentwas using utensil made of aluminum. Keyword: Carrot, Boiling Time, Nitrate, Nitrit, Utensil Type

scholarly journals Differential RNA Sequencing Implicates Sulfide as the Master Regulator of S 0 Metabolism in Chlorobaculum tepidum and Other Green Sulfur Bacteria

2017 ◽  
Vol 84 (3) ◽  
Author(s):  
Jacob M. Hilzinger ◽  
Vidhyavathi Raman ◽  
Kevin E. Shuman ◽  
Brian J. Eddie ◽  
Thomas E. Hanson
Keyword(s):  
Gene Expression ◽  
Elemental Sulfur ◽  
Green Sulfur Bacteria ◽  
Electron Donors ◽  
Reduced Sulfur Compounds ◽  
Promoter Elements ◽  
Content Type ◽  
Sulfur Bacteria ◽  
Chlorobaculum Tepidum ◽  
Green Sulfur

ABSTRACT The green sulfur bacteria ( Chlorobiaceae ) are anaerobes that use electrons from reduced sulfur compounds (sulfide, S 0 , and thiosulfate) as electron donors for photoautotrophic growth. Chlorobaculum tepidum , the model system for the Chlorobiaceae , both produces and consumes extracellular S 0 globules depending on the availability of sulfide in the environment. These physiological changes imply significant changes in gene regulation, which has been observed when sulfide is added to Cba. tepidum growing on thiosulfate. However, the underlying mechanisms driving these gene expression changes, i.e., the specific regulators and promoter elements involved, have not yet been defined. Here, differential RNA sequencing (dRNA-seq) was used to globally identify transcript start sites (TSS) that were present during growth on sulfide, biogenic S 0 , and thiosulfate as sole electron donors. TSS positions were used in combination with RNA-seq data from cultures growing on these same electron donors to identify both basal promoter elements and motifs associated with electron donor-dependent transcriptional regulation. These motifs were conserved across homologous Chlorobiaceae promoters. Two lines of evidence suggest that sulfide-mediated repression is the dominant regulatory mode in Cba. tepidum . First, motifs associated with genes regulated by sulfide overlap key basal promoter elements. Second, deletion of the Cba. tepidum 1277 ( CT1277 ) gene, encoding a putative regulatory protein, leads to constitutive overexpression of the sulfide:quinone oxidoreductase CT1087 in the absence of sulfide. The results suggest that sulfide is the master regulator of sulfur metabolism in Cba. tepidum and the Chlorobiaceae . Finally, the identification of basal promoter elements with differing strengths will further the development of synthetic biology in Cba. tepidum and perhaps other Chlorobiaceae . IMPORTANCE Elemental sulfur is a key intermediate in biogeochemical sulfur cycling. The photoautotrophic green sulfur bacterium Chlorobaculum tepidum either produces or consumes elemental sulfur depending on the availability of sulfide in the environment. Our results reveal transcriptional dynamics of Chlorobaculum tepidum on elemental sulfur and increase our understanding of the mechanisms of transcriptional regulation governing growth on different reduced sulfur compounds. This report identifies genes and sequence motifs that likely play significant roles in the production and consumption of elemental sulfur. Beyond this focused impact, this report paves the way for the development of synthetic biology in Chlorobaculum tepidum and other Chlorobiaceae by providing a comprehensive identification of promoter elements for control of gene expression, a key element of strain engineering.

scholarly journals Temporal fluctuations in the trophic role of large benthic sulfur bacteria in mangrove sediment

Food Webs ◽  
2016 ◽  
Vol 7 ◽  
pp. 20-28 ◽  
Author(s):  
Pierre-Yves Pascal ◽  
Olivier Gros ◽  
Henricus T.S. Boschker
Keyword(s):  
Mangrove Sediment ◽  
Temporal Fluctuations ◽  
Sulfur Bacteria ◽  
Trophic Role

Role of a phenothiazine/phenoxazine donor in solid ionic conductors for efficient solid state dye sensitized solar cells

2017 ◽  
Vol 5 (11) ◽  
pp. 5373-5382 ◽  
Author(s):  
Jayraj V. Vaghasiya ◽  
Keval K. Sonigara ◽  
Jyoti Prasad ◽  
Thomas Beuvier ◽  
Alain Gibaud ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Solar Cell ◽  
Solid State ◽  
Dye Sensitized Solar Cells ◽  
Electron Donors ◽  
Ionic Conductors ◽  
Good Thermal Stability ◽  
Dye Sensitized ◽  
Sensitized Solar Cells

Efficient electron donors, phenothiazine (PTZ)/phenoxazine (POZ) substituted imidazolium (IMI) and benzimidazolium (BIMI) iodide solid organic ionic conductors (SOICs) possessing good thermal stability and high conductivity are synthesized and used as electrolytes in solid state dye solar cell (ss-DSSC).

scholarly journals 1047 PSEUDOMONADS AND THE ROLE OF-THE ANTIBIOTICS IN THE CONTROL OF GREEN MOLD OF LEMONS.

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 578e-578
Author(s):  
J.S. Parham ◽  
J.L. Smilanick
Keyword(s):  
Uv Light ◽  
Inhibition Zone ◽  
Sulfanilic Acid ◽  
Pseudomonas Cepacia ◽  
E Coli ◽  
Green Mold ◽  
Tlc Plates ◽  
Stable Mutant ◽  
Inhibition Zones

Pseudomonas cepacia LT412W reduced green mold on lemons caused by Penicillium digitatum. It produces phenylpyrrole antibiotics which cause inhibition zones in co-culture with the pathogen. Their role in control of the disease was investigated. Mutagenesis of P. cepacia (rifampicin resistant) was performed by mating it with E. coli S-17 pSUP1021 (kanamycin resistant), which carries the transposon Tn5. Transconjugate selection and screening for absence of inhibition zones identified a stable mutant. Growth of parent and mutant were comparable. When the mutant was co-cultured with the pathogen on lemon albedo agar, no inhibition zone appeared. Similar co-culture on potato dextrose agar with tryptophan (0.05 g/L), a precursor of phenylpyrroles, did not induce inhibition zones. This suggests the mutation is not in tryptophan biosynthesis. Parent and mutant were assayed for phenylpyrroles. They were cultured in nutrient broth, centrifuged, and the cells extracted with acetone. The extract was dried and dissolved in chloroform. It was spotted on nano-SIL Cl8 TLC plates, run one hour (methanol:acetonitrile:water, 1:1:1), dried, developed with sulfanilic acid, and observed under UV light. The relative mobility of spots from extracts of the parent matched phenylpyrroles, whereas the mutant produced none. Control of decay by the mutant and parent were equal, suggesting no role for phenylpyrroles in suppression of the disease.

scholarly journals Protective role of new nitrogen compounds on ROS/RNS-mediated damage to PC12 cells

2008 ◽  
Vol 42 (1) ◽  
pp. 57-69 ◽  
Author(s):  
João P. Silva ◽  
Fernanda Proença ◽  
Olga P. Coutinho
Keyword(s):  
Pc12 Cells ◽  
Protective Role ◽  
Nitrogen Compounds

The Role Of Bacteriochlorophyll E And Carotenoids In Light Harvesting In Brown-Colored Green Sulfur Bacteria

1998 ◽  
pp. 149-152 ◽  
Author(s):  
Raymond P. Cox ◽  
Mette Miller ◽  
Jochen Aschenbrücker ◽  
Ying-Zhong Ma ◽  
Tomas Gillbro
Keyword(s):  
Light Harvesting ◽  
Green Sulfur Bacteria ◽  
Sulfur Bacteria ◽  
Green Sulfur ◽  
Bacteriochlorophyll E

The role of electron donors and acceptors in base stacking in DNA and RNA

1990 ◽  
Vol 171 (1) ◽  
pp. 97-101 ◽  
Author(s):  
E.C.M. Chen ◽  
E.S.D. Chen ◽  
W.E. Wentworth
Keyword(s):  
Electron Donors ◽  
Base Stacking ◽  
Dna And Rna
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