scholarly journals Efecto de la cisteína en un proceso de biodepiritización de carbones en lecho empacado

Author(s):  
Gerardo Andrés Caicedo Pineda ◽  
Marco Antonio Márquez Godoy

<p>Se llevaron a cabo procesos de biodesulfurización de dos carbones colombianos ricos en azufre (“Mina Vieja” y “Vampiro”), en reactores de lecho empacado a nivel de erlenmeyer, utilizando un consorcio de Acidithiobacillus ferrooxidans (ATCC 23270) y Acidithiobacillus thiooxidans (ATCC 15494), evaluando la adición de cisteína a la solución lixiviante. Los ensayos fueron monitoreados por medidas de hierro en solución, pH y potencial redox. Adicionalmente, se hicieron análisis mineralógicos por difracción de rayos X (DRX) antes y después de los experimentos. Los ensayos sin adición de cisteína alcanzaron una oxidación de pirita de 45.3% y 57.9% para “Mina Vieja” y “Vampiro” respectivamente. Cuando se adicionó cisteína, la oxidación aumentó en 14.9% para “Mina Vieja” y 6.4% para “Vampiro”. Por otra parte, todos los ensayos evidenciaron remoción de caolinita, debido a su interacción con el ácido sulfúrico del medio. Con base en los resultados obtenidos, los componentes del carbón influenciaron tanto crecimiento bacteriano como la eficiencia de la cisteína sobre el grado de pirita oxidada.</p>

Metabolomics ◽  
2012 ◽  
Vol 9 (1) ◽  
pp. 247-257 ◽  
Author(s):  
Patricio Martínez ◽  
Sebastián Gálvez ◽  
Norimasa Ohtsuka ◽  
Marko Budinich ◽  
María Paz Cortés ◽  
...  

Energies ◽  
2021 ◽  
Vol 14 (11) ◽  
pp. 3336
Author(s):  
Tomasz Kamizela ◽  
Anna Grobelak ◽  
Malgorzata Worwag

Among the methods used to remove metals and their compounds from landfill leachates with low application costs and high efficiency are bioleaching and biosorption. The most effective bacteria used in the metal removal process are Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans. The aim of the study was to determine the usefulness of the A. ferrooxidans and A. thiooxidans population in removing heavy metals from landfill leachate. In addition, development opportunities for bacterial population using landfill leachate as growth medium were identified. The substrate for the research was the raw leachate before the reverse osmosis process. In order to increase the efficiency of trace elements removal and recovery from leachate, variable combinations have been used which differ by the addition of sulfuric acid, A. ferrooxidans culture, A. thiooxidans culture, mixed culture containing populations of both bacteria, and elemental sulfur. Based on the research, it was found that the removal of heavy metals from leachate was a selective process. High bioleaching efficiency, from 80% to 90%, was obtained for all metals for which the sample acidification or sulfur addition was used. The simultaneous combination of both these additives turned out to be the most advantageous. The A. thiooxidans culture was the most effective in bioleaching reverse osmosis effluents. For the A. ferrooxidans culture used, much lower efficiencies were obtained, while by contrast, the use of mixed culture of two bacterium species had no significant effect.


Respuestas ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 72-83
Author(s):  
Lina M. Miranda Arroyave ◽  
Marco Antonio Márquez Godoy ◽  
Luz Marina Ocampo Carmona

One of the main characteristics of the microorganisms used in the leaching process is their capacity to adapt to aggressive environments, characterized by a notable presence of heavy metals. In this study the adaptation of the strains Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Leptospirillum ferrooxidans was evaluated on a sphalerite concentrate from mining waste. In the adaptation tests, the energy source (ferrous sulphate) was gradually replaced by percentages of mineral pulp, ending with subcultures without the addition of an external energy source. The results show that the strains A. ferrooxidans and A. thiooxidans are more resistant to high concentrations of sphalerite, compared to the strain of L. ferrooxidans, since, in the case of this strain, it was necessary to repeat some tests (8% of pulp), since a deficient development was evident. This was associated with factors such as the decrease of the Fe+2 energy source, the increase of the pulp density, the accumulation of toxic metals and secondary products of the dissolution of minerals and the increase of the pH.


DYNA ◽  
2016 ◽  
Vol 83 (197) ◽  
pp. 74
Author(s):  
Gerardo Andrés Caicedo Pineda ◽  
Marco Antonio Márquez Godoy

<p>Se evaluaron tres fuentes de hierro (FeSO4, Fe2(SO4)3 y FeCl3) a diferentes concentraciones (150, 700 y 1250 mg Fe/L) en procesos de biodesulfurización de un carbón con tamaño de partícula grueso, utilizando un consorcio de Acidithiobacillus ferrooxidans (ATCC 23270) and Acidithiobacillus thiooxidans (ATCC 15494). Al usar 150 mg Fe2+/L (FeSO4), luego de 12 días se obtuvo 31.14% de pirita oxidada. Al reemplazar FeSO4 por Fe2(SO4)3, la oxidación mejoró en un 21.16%. Aunque los ensayos con las mayores concentraciones de sulfatos obtuvieron un resultado similar, al utilizar Fe2(SO4)3 permitió mejor remoción de sulfatos del carbón. Lo anterior sugiere que basta con utilizar la menor concentración de Fe2(SO4)3 para mejorar el índice de oxidación de pirita y evitar formación de precipitados. Por otra parte, los ensayos con FeCl3 tuvieron una disminución en la tasa de biooxidación, indicando a priori, que los microorganismos no fueron capaces de adaptarse adecuadamente a los iones Cl-.</p>


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