scholarly journals Aislamiento e identificación de microorganismos potencialmente amilolíticos y celulolíticos de suelos de humedales de Bogotá

2020 ◽  
Vol 22 (1) ◽  
pp. 36-44
Author(s):  
Etna Milena Sánchez Castelblanco ◽  
Juan Pablo Heredia Martín ◽  
Sonia Marcela Buitrago Morales ◽  
Juan Pablo Medina Rodríguez
Keyword(s):  
Stenotrophomonas Nitritireducens

Las amilasas y celulasas de origen microbiano se han utilizado desde hace más de tres décadas en la industria; el aislamiento de cepas microbianas nativas productoras de enzimas es el punto de partida para aprovechar la biodiversidad microbiana para la obtención de enzimas con propiedades para la obtención de nuevos productos en la optimización de procesos industriales. El objetivo de este trabajo, fue aislar, a partir de suelo de cinco humedales en Bogotá, cepas microbianas con capacidad para producir enzimas amilolíticas y celulolíticas. Se realizó la medición de halos de hidrólisis en agar almidón y agar carboximetilcelulosa. Se determinaron las unidades enzimáticas (U/ml/min) por medio de azúcares reductores por la técnica de DNS. Se seleccionaron cuatro cepas de Bacillus subtillis amiloliquefaciens productoras de amilasas con actividades entre 752±33 y 480 ± 35 U/ml/min a 60ºC y cinco cepas celulolíticas identificadas como Bacillus subtillis amiloliquefaciens, Stenotrophomonas nitritireducens y Yersinia massiliensis, capaces de producir celulasas con actividades enzimáticas entre 19.11 ± 2.3 y 13.82 ± 2.5 UA/ml/min a 50ºC. Con estos resultados se demostró que a partir de suelos de humedales, se pueden recuperar microrganismos con capacidad de producción enzimática, como punto de partida para una posterior aplicación en procesos industriales.

scholarly journals Screening for endophytic nitrogen-fixing bacteria in Brazilian sugar cane varieties used in organic farming and description of Stenotrophomonas pavanii sp. nov.

2011 ◽  
Vol 61 (4) ◽  
pp. 926-931 ◽  
Author(s):  
Patrícia L. Ramos ◽  
Stefanie Van Trappen ◽  
Fabiano L. Thompson ◽  
Rafael C. S. Rocha ◽  
Heloiza R. Barbosa ◽  
...  
Keyword(s):  
Organic Farming ◽  
Sugar Cane ◽  
Dna Hybridization ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Nitrogen Fixing ◽  
Gene Sequence Analysis ◽  
Stenotrophomonas Rhizophila ◽  
Stenotrophomonas Nitritireducens

A Gram-negative, rod-shaped, non-spore-forming and nitrogen-fixing bacterium, designated ICB 89T, was isolated from stems of a Brazilian sugar cane variety widely used in organic farming. 16S rRNA gene sequence analysis revealed that strain ICB 89T belonged to the genus Stenotrophomonas and was most closely related to Stenotrophomonas maltophilia LMG 958T, Stenotrophomonas rhizophila LMG 22075T, Stenotrophomonas nitritireducens L2T, [Pseudomonas] geniculata ATCC 19374T, [Pseudomonas] hibiscicola ATCC 19867T and [Pseudomonas] beteli ATCC 19861T. DNA–DNA hybridization together with chemotaxonomic data and biochemical characteristics allowed the differentiation of strain ICB 89T from its nearest phylogenetic neighbours. Therefore, strain ICB 89T represents a novel species, for which the name Stenotrophomonas pavanii sp. nov. is proposed. The type strain is ICB 89T ( = CBMAI 564T  = LMG 25348T).

scholarly journals Bacterium-Based NO2− Biosensor for Environmental Applications

2004 ◽  
Vol 70 (11) ◽  
pp. 6551-6558 ◽  
Author(s):  
Michael Nielsen ◽  
Lars Hauer Larsen ◽  
Mike S. M. Jetten ◽  
Niels Peter Revsbech
Keyword(s):  
Growth Rate ◽  
Online Monitoring ◽  
Reductase Activity ◽  
Alcaligenes Faecalis ◽  
Sensor Signal ◽  
Bacterial Reduction ◽  
Reduction Of No ◽  
Marine Systems ◽  
Stenotrophomonas Nitritireducens

ABSTRACT A sensitive NO2 − biosensor that is based on bacterial reduction of NO2 − to N2O and subsequent detection of the N2O by a built-in electrochemical N2O sensor was developed. Four different denitrifying organisms lacking NO3 − reductase activity were assessed for use in the biosensor. The relevant physiological aspects examined included denitrifying characteristics, growth rate, NO2 − tolerance, and temperature and salinity effects on the growth rate. Two organisms were successfully used in the biosensor. The preferred organism was Stenotrophomonas nitritireducens, which is an organism with a denitrifying pathway deficient in both NO3 − and N2O reductases. Alternatively Alcaligenes faecalis could be used when acetylene was added to inhibit its N2O reductase. The macroscale biosensors constructed exhibited a linear NO2 − response at concentrations up to 1 to 2 mM. The detection limit was around 1 μM NO2 −, and the 90% response time was 0.5 to 3 min. The sensor signal was specific for NO2 −, and interference was observed only with NH2OH, NO, N2O, and H2S. The sensor signal was affected by changes in temperature and salinity, and calibration had to be performed in a system with a temperature and an ionic strength comparable to those of the medium analyzed. A broad range of water bodies could be analyzed with the biosensor, including freshwater systems, marine systems, and oxic-anoxic wastewaters. The NO2 − biosensor was successfully used for long-term online monitoring in wastewater. Microscale versions of the NO2 − biosensor were constructed and used to measure NO2 − profiles in marine sediment.

scholarly journals Stenotrophomonas ginsengisoli sp. nov., isolated from a ginseng field

2010 ◽  
Vol 60 (7) ◽  
pp. 1522-1526 ◽  
Author(s):  
Ho-Bin Kim ◽  
Sathiyaraj Srinivasan ◽  
Gayathri Sathiyaraj ◽  
Lin-Hu Quan ◽  
Se-Hwa Kim ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Biochemical Tests ◽  
Gene Sequence Analysis ◽  
Stenotrophomonas Nitritireducens ◽  
Physiological And Biochemical

A Gram-negative, non-spore-forming, rod-shaped bacterium, designated strain DCY01T, was isolated from soil from a ginseng field in South Korea and was characterized in order to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain DCY01T belonged to the Gammaproteobacteria and was most closely related to Stenotrophomonas koreensis KCTC 12211T (98.4 % similarity), Stenotrophomonas humi R-32729T (97.2 %), Stenotrophomonas terrae R-32768 (97.1 %), Stenotrophomonas maltophilia DSM 50170T (96.9 %) and Stenotrophomonas nitritireducens DSM 12575T (96.8 %). Chemotaxonomic analyses revealed that strain DCY01T possessed a quinone system with Q-8 as the predominant compound, and iso-C15 : 0 (28.2 %), C16 : 0 10-methyl (13.2 %), iso-C15 : 1 F (10.8 %) and C15 : 0 (7.5 %) as major fatty acids, corroborating assignment of strain DCY01T to the genus Stenotrophomonas. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The results of DNA–DNA hybridization and physiological and biochemical tests clearly demonstrated that strain DCY01T represents a species distinct from recognized Stenotrophomonas species. Based on these data, DCY01T (=KCTC 12539T=NBRC 101154T) should be classified as the type strain of a novel species of the genus Stenotrophomonas, for which the name Stenotrophomonas ginsengisoli sp. nov. is proposed.

scholarly journals Antimicrobial susceptibility pattern of Stenotrophomonas species isolated from Mexico

2020 ◽  
Vol 20 (1) ◽  
pp. 168-181
Author(s):  
Temidayo O Elufisan ◽  
Isabel Cristina Rodriguez-Luna ◽  
Omotayo O Oyedara ◽  
Alejandro Sanchez-Varela ◽  
Virgilio Bocanegra García ◽  
...  
Keyword(s):  
Bacterial Resistance ◽  
Antimicrobial Agents ◽  
Biochemical Characterization ◽  
Opportunistic Infections ◽  
Clonal Diversity ◽  
Resistant Bacteria ◽  
Rrna Gene ◽  
Antimicrobial Susceptibility Pattern ◽  
Concentration Technique ◽  
Stenotrophomonas Nitritireducens

Background: Stenotrophomonas species are multi-resistant bacteria with ability to cause opportunistic infections. Objective: We isolated 45 Stenotrophomonas species from soil, sewage and the clinic with the aim of investigating their susceptibility to commonly used antimicrobial agents. Methodology: The identities of isolates were confirmed with 16S rRNA gene sequence and MALDI-TOF analysis. Anti-mi- crobial resistance, biofilm production and clonal diversity were also evaluated. The minimum inhibitory concentration technique as described by Clinical & Laboratory Standards Institute: CLSI Guidelines (CLSI) was employed for the evaluation of isolate susceptibility to antibiotics. Result: Forty-five Stenotrophomonas species which include 36 environmental strains and 9 clinical strains of S. maltophilia were considered in this study. 32 (88.9 %) environmental strains were identified to be S. maltophilia, 2 (5.6 %) were Stenotrophomonas nitritireducens, and 2 (5.6 %) cluster as Stenotrophomonas spp. Stenotrophomonas isolates were resistant to at least six of the antibiotics tested, including Trimethoprim/Sulfamethoxazole (SXT). Conclusion: Environmental isolates from this study were resistant to SXT which is commonly used for the treatment of S. maltophilia infections. This informs the need for good public hygiene as the environment could be a reservoir of multi-resistant bacteria. It also buttresses the importance of surveillance study in the management of bacterial resistance. Keywords: Stenotrophomonas; environmental; biochemical characterization; clonal diversity; anti-microbial susceptibility.  

Exemplar Abstract for Stenotrophomonas nitritireducens Finkmann et al. 2000.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Nicole Danielle Osier ◽  
George M Garrity
Keyword(s):  
Stenotrophomonas Nitritireducens
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