Tryptase- and ghrelin positive mast cells in the interalveolar septa of rat’s lung

The mast cell mediators and distribution of lung mast cells in rats are often discussed in experimental studies on pulmonary fibrotic and allergic processes associated with changes in numbers of these cells, but information on the normal distribution of metachromatic and tryptase-positive mast cells in the interalveolar septa is scarce. There are no data on the presence of ghrelin in lung mast cells as well as the age-specific features of localisation and the number of mast cells in the interalveolar septa in rats of different ages. Therefore, the purpose of the present study was to determine the distribution of metachromatic, tryptase-, and ghrelin-positive mast cells in the interalveolar septa in 20 day-, 3 month- and 1 year-old rats. Tissue sections stained with toluidine blue had been taken from the left lung to visualise metachromasia and immunohistochemical expression of tryptase and ghrelin. The results showed that the amount of metachromatic mast cells in the interalveolar septa was significantly lower than that of tryptase- and ghrelin-positive cells. This allowed suggesting that mast cells were permanent occupants of the rat lung parenchyma and, on the other hand, the expression of ghrelin in their granules was most likely related to the synthesis of this protein. Our study showed that immunohistochemical identification by tryptase expression was more accurate than toluidine blue staining.

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Toluidine blue staining of murine mast cells and quantitation by a novel, automated image analysis method using whole slide skin images

Journal of Histotechnology ◽

10.1080/01478885.2021.1915934 ◽

2021 ◽

pp. 1-6

Author(s):

Nivethitha Raja ◽

Sangmesh Naikodi ◽

Arunprasath Govindarajan ◽

Kamalavenkatesh Palanisamy

Keyword(s):

Image Analysis ◽

Mast Cells ◽

Toluidine Blue ◽

Automated Image Analysis ◽

Blue Staining ◽

Analysis Method ◽

Image Analysis Method ◽

Toluidine Blue Staining

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METACHROMASIA INHIBITING COMPONENTS IN AMYLOID

Journal of Histochemistry & Cytochemistry ◽

10.1177/6.3.181 ◽

1958 ◽

Vol 6 (3) ◽

pp. 181-184 ◽

Cited By ~ 11

Author(s):

BØRGE LARSEN

Keyword(s):

Chondroitin Sulfate ◽

Toluidine Blue ◽

Serum Proteins ◽

Periodic Acid ◽

Methyl Violet ◽

Inhibitory Effect ◽

Blue Staining ◽

Periodic Acid Schiff ◽

Toluidine Blue Staining ◽

Tissue Sections

I): The degree of inhibitory effect of serum proteins and a periodic acid Schiff-positive amyloid fraction on metachromasia resulting from a metachromatic amyloid fraction was studied and compared to the metachromatic potency of chondroitin sulfate exposed to the same type of inhibition. The metachromatic properties of chondroitin sulfate were close to those of the metachromatic amyloid fraction. II): In comparing the metachromasia obtained with toluidine blue and methyl violet, it was noted that on incubation with the periodic acid Schiff-positive amyloid fraction, amyloid metachromosia after methyl violet staining was inhibited less than after toluidine blue staining. III): It is emphasized that a competition between dye and proteins occurs in metachromatic staining reactions. IV): Inhibition of amyloid metachromasia in histological tissue sections could not be demonstrated with the techniques used.

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Detection of Infiltrating Mast Cells Using a Modified Toluidine Blue Staining

Fibrosis - Methods in Molecular Biology ◽

10.1007/978-1-4939-7113-8_14 ◽

2017 ◽

pp. 213-222 ◽

Cited By ~ 10

Author(s):

Nahum Puebla-Osorio ◽

Seri N. E. Sarchio ◽

Stephen E. Ullrich ◽

Scott N. Byrne

Keyword(s):

Mast Cells ◽

Toluidine Blue ◽

Blue Staining ◽

Toluidine Blue Staining

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Distribution of Mast Cell in Acute Appendicitis and Its Role in Histopathological Diagnosis - A Study in Tertiary Care Centre from South India

Journal of Evidence Based Medicine and Healthcare ◽

10.18410/jebmh/2021/373 ◽

2021 ◽

Vol 8 (23) ◽

pp. 1988-1992

Author(s):

Syamini Chandrasekharapillai Leelamany ◽

Seena Abdul Vaheed Radhika Devi

Keyword(s):

Mast Cell ◽

Mast Cells ◽

Acute Appendicitis ◽

Toluidine Blue ◽

South India ◽

Tertiary Care ◽

Blue Staining ◽

Cell Distribution ◽

Toluidine Blue Staining ◽

Cd 117

BACKGROUND Appendicitis is the most common indication for intra-abdominal surgery, especially in adolescents and young adults. In about 15 – 25 % of appendices removed at surgery because of suspected symptoms, histological features of acute appendicitis are absent. The cause of acute abdominal pain in these patients can be due to mast cells which may play a role in pathogenesis of appendicitis-like pain. Demonstration of mast cell in tissue can be done by toluidine blue staining or immunohistochemistry (IHC) marker CD 117. We wanted to evaluate whether the degree of distribution of mast cells has any relation with clinical findings and evaluate its usefulness as a diagnostic marker for histological diagnosis of acute appendicitis. METHODS This is a descriptive study done in 120 cases of appendicectomy specimens from clinically diagnosed cases of acute appendicitis received in a tertiary care center in South India in which 60 where histology positive and others were histology negative. Mast cell distribution in each group was compared using toluidine blue stain and CD 117. Collected data was entered in Microsoft excel and analysed using the statistical software SPSS version 16. RESULTS Mast cell distribution was significant in all layers of histologically negative acute appendicitis in comparison with histology positive cases. Mucosa has maximum mast cells distribution. CONCLUSIONS Mast cells play an important role in the clinical symptoms of patients even when there were no features of acute inflammation. In those cases, mast cells can be demonstrated by simple toluidine blue staining or IHC markers. This is one of the less studied areas, and the clinicopathological discrepancy can be solved by giving mast cell distribution along with histopathology diagnosis. KEYWORDS Appendicitis, Histologically Negative Acute Appendicitis, Mast Cells, Toluidine Blue, CD 117

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Combination of toluidine blue staining and in situ hybridization to evaluate paraffin tissue sections

Cancer Genetics and Cytogenetics ◽

10.1016/j.cancergencyto.2004.03.019 ◽

2004 ◽

Vol 155 (1) ◽

pp. 89-91 ◽

Cited By ~ 1

Author(s):

Esther Prat ◽

Jordi Camps ◽

Javier del Rey ◽

Josep Egozcue ◽

Rosa Miró ◽

...

Keyword(s):

In Situ Hybridization ◽

Toluidine Blue ◽

Blue Staining ◽

Toluidine Blue Staining ◽

Tissue Sections ◽

Paraffin Tissue

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Ultrafast Toluidine Blue Staining for Rapid On-Site Evaluation of Cytological Smears

Acta Cytologica ◽

10.1159/000505254 ◽

2020 ◽

Vol 64 (4) ◽

pp. 375-377

Author(s):

Ekkehard Hewer ◽

Anja M. Schmitt

Keyword(s):

Organic Solvents ◽

Toluidine Blue ◽

Critical Factor ◽

Blue Staining ◽

Nuclear Morphology ◽

Hazardous Chemicals ◽

Toluidine Blue Staining ◽

Site Evaluation ◽

The Rose

Rapid on-site evaluation (ROSE) is one of cytopathology’s “unique selling propositions.” The quality, speed, and ease of handling of the staining used is a critical factor for the efficacy of the ROSE procedure. Here, we describe a modification of rapid toluidine blue staining that can be performed within 25 s, provides excellent nuclear morphology, and is compatible with subsequent Papanicolaou staining of the slides. Furthermore, exposure to hazardous chemicals is minimized, as no organic solvents other than the alcohol-based fixative and glycerin for temporary mounting and coverslipping are required. We have used this protocol successfully in our ROSE practice and have not observed any discrepancies between toluidine blue- and permanent Papanicolaou-stained slides.

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