scholarly journals Detection of Infiltrating Mast Cells Using a Modified Toluidine Blue Staining

2017 ◽  
pp. 213-222 ◽  
Author(s):  
Nahum Puebla-Osorio ◽  
Seri N. E. Sarchio ◽  
Stephen E. Ullrich ◽  
Scott N. Byrne
Keyword(s):  
Mast Cells ◽  
Toluidine Blue ◽  
Blue Staining ◽  
Toluidine Blue Staining

scholarly journals Tryptase- and ghrelin positive mast cells in the interalveolar septa of rat’s lung

2021 ◽  
Vol 24 (4) ◽  
pp. 469-477
Author(s):  
I. G. Ivanova ◽  
I. S. Stefanov
Keyword(s):  
Mast Cells ◽  
Toluidine Blue ◽  
Experimental Studies ◽  
Left Lung ◽  
Lung Parenchyma ◽  
Blue Staining ◽  
Toluidine Blue Staining ◽  
Tissue Sections ◽  
Old Rats ◽  
Immunohistochemical Identification

The mast cell mediators and distribution of lung mast cells in rats are often discussed in experimental studies on pulmonary fibrotic and allergic processes associated with changes in numbers of these cells, but information on the normal distribution of metachromatic and tryptase-positive mast cells in the interalveolar septa is scarce. There are no data on the presence of ghrelin in lung mast cells as well as the age-specific features of localisation and the number of mast cells in the interalveolar septa in rats of different ages. Therefore, the purpose of the present study was to determine the distribution of metachromatic, tryptase-, and ghrelin-positive mast cells in the interalveolar septa in 20 day-, 3 month- and 1 year-old rats. Tissue sections stained with toluidine blue had been taken from the left lung to visualise metachromasia and immunohistochemical expression of tryptase and ghrelin. The results showed that the amount of metachromatic mast cells in the interalveolar septa was significantly lower than that of tryptase- and ghrelin-positive cells. This allowed suggesting that mast cells were permanent occupants of the rat lung parenchyma and, on the other hand, the expression of ghrelin in their granules was most likely related to the synthesis of this protein. Our study showed that immunohistochemical identification by tryptase expression was more accurate than toluidine blue staining.

scholarly journals Distribution of Mast Cell in Acute Appendicitis and Its Role in Histopathological Diagnosis - A Study in Tertiary Care Centre from South India

2021 ◽  
Vol 8 (23) ◽  
pp. 1988-1992
Author(s):  
Syamini Chandrasekharapillai Leelamany ◽  
Seena Abdul Vaheed Radhika Devi
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Acute Appendicitis ◽  
Toluidine Blue ◽  
South India ◽  
Tertiary Care ◽  
Blue Staining ◽  
Cell Distribution ◽  
Toluidine Blue Staining ◽  
Cd 117

BACKGROUND Appendicitis is the most common indication for intra-abdominal surgery, especially in adolescents and young adults. In about 15 – 25 % of appendices removed at surgery because of suspected symptoms, histological features of acute appendicitis are absent. The cause of acute abdominal pain in these patients can be due to mast cells which may play a role in pathogenesis of appendicitis-like pain. Demonstration of mast cell in tissue can be done by toluidine blue staining or immunohistochemistry (IHC) marker CD 117. We wanted to evaluate whether the degree of distribution of mast cells has any relation with clinical findings and evaluate its usefulness as a diagnostic marker for histological diagnosis of acute appendicitis. METHODS This is a descriptive study done in 120 cases of appendicectomy specimens from clinically diagnosed cases of acute appendicitis received in a tertiary care center in South India in which 60 where histology positive and others were histology negative. Mast cell distribution in each group was compared using toluidine blue stain and CD 117. Collected data was entered in Microsoft excel and analysed using the statistical software SPSS version 16. RESULTS Mast cell distribution was significant in all layers of histologically negative acute appendicitis in comparison with histology positive cases. Mucosa has maximum mast cells distribution. CONCLUSIONS Mast cells play an important role in the clinical symptoms of patients even when there were no features of acute inflammation. In those cases, mast cells can be demonstrated by simple toluidine blue staining or IHC markers. This is one of the less studied areas, and the clinicopathological discrepancy can be solved by giving mast cell distribution along with histopathology diagnosis. KEYWORDS Appendicitis, Histologically Negative Acute Appendicitis, Mast Cells, Toluidine Blue, CD 117

Ultrafast Toluidine Blue Staining for Rapid On-Site Evaluation of Cytological Smears

2020 ◽  
Vol 64 (4) ◽  
pp. 375-377
Author(s):  
Ekkehard Hewer ◽  
Anja M. Schmitt
Keyword(s):  
Organic Solvents ◽  
Toluidine Blue ◽  
Critical Factor ◽  
Blue Staining ◽  
Nuclear Morphology ◽  
Hazardous Chemicals ◽  
Toluidine Blue Staining ◽  
Site Evaluation ◽  
The Rose

Rapid on-site evaluation (ROSE) is one of cytopathology’s “unique selling propositions.” The quality, speed, and ease of handling of the staining used is a critical factor for the efficacy of the ROSE procedure. Here, we describe a modification of rapid toluidine blue staining that can be performed within 25 s, provides excellent nuclear morphology, and is compatible with subsequent Papanicolaou staining of the slides. Furthermore, exposure to hazardous chemicals is minimized, as no organic solvents other than the alcohol-based fixative and glycerin for temporary mounting and coverslipping are required. We have used this protocol successfully in our ROSE practice and have not observed any discrepancies between toluidine blue- and permanent Papanicolaou-stained slides.

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