Colonisation of first-year maize fields by western corn rootworm (Diabrotica virgifera virgiferaLeConte) from adjacent infested maize fields

2011 ◽  
Vol 46 (2) ◽  
pp. 213-223 ◽  
Author(s):  
M. Szalai ◽  
J. Kőszegi ◽  
S. Toepfer ◽  
J. Kiss
Keyword(s):  
Western Corn Rootworm ◽  
First Year ◽  
Corn Rootworm ◽  
Diabrotica Virgifera

Eastward expansion and discovery of the soybean biotype of western corn rootworm (Diabrotica virgifera virgifera LeConte) in Canada

2004 ◽  
Vol 84 (1) ◽  
pp. 305-309 ◽  
Author(s):  
François Meloche ◽  
Paul Hermans
Keyword(s):  
Key Words ◽  
Species Distribution ◽  
Diabrotica Virgifera Virgifera ◽  
Western Corn Rootworm ◽  
First Year ◽  
Corn Rootworm ◽  
Diabrotica Virgifera ◽  
First Report ◽  
Emergence Traps ◽  
Diabrotica Virgifera Virgifera Leconte

The western corn rootworm (WCR), (Diabrotica virgifera virgifera LeConte), was collected in eastern Ontario and western Quebec in 2000. At Ottawa and North Gower, WCR adults were recovered using emergence traps from first-year corn and, at Ottawa, from first year soybean after corn. This constitutes an extension of the species distribution and the first report of WCR developing in soybean in Canada. Key words: Western corn rootworm, Diabrotica virgifera virgifera, distribution, corn, soybean

scholarly journals Detection of alternative splicing in Diabrotica virgifera virgifera LeConte, in association with Bt resistance using RNA-seq and PacBio Iso-Seq

2020 ◽  
Author(s):  
Zixiao Zhao ◽  
Christine G. Elsik ◽  
Bruce E. Hibbard ◽  
Kent S. Shelby
Keyword(s):  
Alternative Splicing ◽  
Matrix Protein ◽  
Diabrotica Virgifera Virgifera ◽  
Western Corn Rootworm ◽  
Corn Rootworm ◽  
Diabrotica Virgifera ◽  
Rna Seq ◽  
Bt Resistance ◽  
Bt Toxins ◽  
Alternatively Spliced

AbstractBackgroundAlternative splicing is one of the major mechanisms that increases transcriptome diversity in eukaryotes, including insect species that have gained resistance to pesticides and Bt toxins. In western corn rootworm (Diabrotica virgifera virgifera LeConte), neither alternative splicing nor its role in resistance to Bt toxins has been studied.ResultsTo investigate the mechanisms of Bt resistance we carried out single-molecule real-time (SMRT) transcript sequencing and Iso-seq analysis on resistant, eCry3.1Ab-selected and susceptible, unselected, western corn rootworm neonate midguts which fed on seedling maize with and without eCry3.1Ab for 12 and 24 hours. We present transcriptome-wide alternative splicing patterns of western corn rootworm midgut in response to feeding on eCry3.1Ab-expressing corn using a comprehensive approach that combines both RNA-seq and SMRT transcript sequencing techniques. We found that 67.73% of multi-exon genes are alternatively spliced, which is consistent with the high transposable element content of the genome. One of the alternative splicing events we identified was a novel peritrophic matrix protein with two alternative splicing isoforms. Analysis of differential exon usage between resistant and susceptible colonies showed that in eCry3.1Ab-resistant western corn rootworm, expression of one isoform was significantly higher than in the susceptible colony, while no significant differences between colonies were observed with the other isoform.ConclusionOur results provide the first survey of alternative splicing in western corn rootworm and suggest that the observed alternatively spliced isoforms of peritrophic matrix protein may be associated with eCry3.1Ab resistance in western corn rootworm.

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