DNA sequence of a small, unidentified plasmid isolated from a Haemophilus somnus strain: Short communication

One of the plasmids present in a Haemophilus somnus strain isolated from nasal discharge of a cattle with respiratory disease was purified and cloned for DNA sequencing. The plasmid was found to be 1065 base pairs long with 39.2% G+C content, and showed no homology to any DNA sequenced so far. It has no capacity to code any protein longer than 43 residues. It is not clear yet if this plasmid codes Haemophilus somnus specific factors.

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Short Communication DNA sequence variants in the carbonyl reductase 1 (cbr1) gene in seven breeds of Canis lupus familiaris

Genetics and Molecular Research ◽

10.4238/2012.april.27.10 ◽

2012 ◽

Vol 11 (2) ◽

pp. 1109-1116 ◽

Cited By ~ 2

Author(s):

Q. Cheng ◽

C. Sanborn ◽

D. Ferguson ◽

J.G. Blanco

Keyword(s):

Dna Sequence ◽

Canis Lupus ◽

Short Communication ◽

Carbonyl Reductase ◽

Sequence Variants ◽

Canis Lupus Familiaris ◽

Dna Sequence Variants

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Isolation of Haemophilus somnus from dairy cattle in KwaZulu-Natal : an emerging cause of 'dirty cow syndrome' and infertility? : short communication

Journal of the South African Veterinary Association ◽

10.4102/jsava.v72i2.625 ◽

2001 ◽

Vol 72 (2) ◽

Cited By ~ 5

Author(s):

R.D. Last ◽

M.D. Macfarlane ◽

C.J. Jarvis

Keyword(s):

North America ◽

Dairy Cattle ◽

Dairy Cows ◽

Artificial Insemination ◽

Short Communication ◽

Cure Rate ◽

Kwazulu Natal ◽

Haemophilus Somnus ◽

High Employment ◽

Natural Breeding

Haemophilus somnus was consistently isolated from vaginal discharges of dairy cows submitted from field cases of vaginitis, cervicitis and/or metritis in the KwaZulu-Natal Midlands during the period July 1995 - December 2000 and from the East Griqualand area in November/December 2000. The purulent vaginal discharges, red granular vaginitis and cervicitis, and pain on palpation described in these cases was very similar to that reported in outbreaks of H.somnus endometritis syndrome in Australia, Europe and North America. In all the herds involved in these outbreaks, natural breeding with bulls was employed. Although there was a good cure rate in clinically-affected animals treated with tetracyclines, culling rates for chronic infertility were unacceptably high. Employment of artificial insemination in these herds improved pregnancy rates in cows that had calved previously, but many cows that had formerly been infected failed to conceive.

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Ultrastructure of primary spermatocyte in fish (Tilapia: Oreochromis niloticus): The synaptonemal complex

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100143067 ◽

1986 ◽

Vol 44 ◽

pp. 288-289

Author(s):

T. Guha ◽

A. Q. Siddiqui ◽

P. F. Prentis

Keyword(s):

Fine Structure ◽

Electron Microscope ◽

Synaptonemal Complex ◽

Oreochromis Niloticus ◽

Short Communication ◽

Primary Spermatocyte ◽

Mitotic Division ◽

Meiotic Cell ◽

Electron Microscope Level ◽

Homologous Chromosomes

The Primary Spermatocytes represent a stage in spermatogenesis when the first meiotic cell division occurs. They are derived from Spermatogonium or Stem cell through mitotic division. At the zygotene phase of meiotic prophase the Synaptonemal complex appears in these cells in the space between the paired homologous chromosomes. Spermatogenesis and sperm structure in fish have been studied at the electron microscope level in a few species? However, no work has yet been reported on ultrastructure of tilapia, O. niloticus, spermatozoa and spermatogenetic process. In this short communication we are reporting the Ultrastructure of Primary Spermatocytes in tilapia, O. niloticus, and the fine structure of synaptonemal complexes seen in the spermatocyte nuclei.

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DNA sequence mapping in interphase and metaphase chromosomes by fluorescence in situ hybridization

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100122885 ◽

1992 ◽

Vol 50 (1) ◽

pp. 496-497

Author(s):

Barbara Trask ◽

Susan Allen ◽

Anne Bergmann ◽

Mari Christensen ◽

Anne Fertitta ◽

...

Keyword(s):

In Situ Hybridization ◽

Dna Sequence ◽

Dna Sequences ◽

Dual Band ◽

Nick Translation ◽

Metaphase Chromosomes ◽

Band Pass ◽

Texas Red ◽

Fluorescent Spot

Using fluorescence in situ hybridization (FISH), the positions of DNA sequences can be discretely marked with a fluorescent spot. The efficiency of marking DNA sequences of the size cloned in cosmids is 90-95%, and the fluorescent spots produced after FISH are ≈0.3 μm in diameter. Sites of two sequences can be distinguished using two-color FISH. Different reporter molecules, such as biotin or digoxigenin, are incorporated into DNA sequence probes by nick translation. These reporter molecules are labeled after hybridization with different fluorochromes, e.g., FITC and Texas Red. The development of dual band pass filters (Chromatechnology) allows these fluorochromes to be photographed simultaneously without registration shift.

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