A survey for bvdv antibodies in cattle farms in Slovakia and genetic typing of bvdv isolates from imported animals

2003 ◽  
Vol 51 (2) ◽  
pp. 229-236 ◽  
Author(s):  
Š. Vilček ◽  
Jana Mojžišová ◽  
Viera Bajová ◽  
Š. Paulík ◽  
L. Strojný ◽  
...  
Keyword(s):  
Bovine Viral Diarrhoea Virus ◽  
Computer Assisted ◽  
Serological Survey ◽  
Bovine Viral Diarrhoea ◽  
Rt Pcr ◽  
Serum Samples ◽  
Genetic Typing ◽  
Diarrhoea Virus ◽  
Pcr Products ◽  
Cattle Farms

A serological survey for bovine viral diarrhoea virus (BVDV) antibodies on a collection of 1295 serum samples obtained from 6-12 months old cattle originating from 45 farms in Slovakia was carried out. On 13 farms more than 90% of the examined animals were seropositive, on 14 farms 71-90% seroprevalence was observed, on 13 farms only 50-70% animals were found to be positive for BVDV antibodies, while the remaining 5 farms showed fewer than 50% seropositive animals. The average incidence of BVDV antibodies (around 70%) was similar as determined 30 years ago. Of 84 serum samples from seronegative animals originating from 14 farms in which 70-98% seropositivity was observed, six were positive in Ag-BVDV ELISA indicating persistently infected (PI) cattle. On a farm to which animals were imported from abroad, a BVD outbreak was observed. Of 110 animals tested, four were positive in Ag-ELISA indicating the presence of PI cattle on this farm. Genetic typing of two isolates from imported animals performed by RT-PCR (324/326 primers from 5´-UTR), sequencing of PCR products and computer-assisted phylogenetic analysis revealed that they belong to BVDV-1h group.

scholarly journals Genotyping of bovine viral diarrhoea virus isolates from the Czech Republic

2009 ◽  
Vol 54 (No. 9) ◽  
pp. 393-398 ◽  
Author(s):  
B. Robesova ◽  
K. Kovarcik ◽  
S. Vilcek
Keyword(s):  
Czech Republic ◽  
Bovine Viral Diarrhoea Virus ◽  
Computer Assisted ◽  
Bovine Viral Diarrhoea ◽  
Rt Pcr ◽  
Serum Samples ◽  
The Czech Republic ◽  
Genetic Typing ◽  
Diarrhoea Virus ◽  
Virus Isolates

This study was focused on the genetic typing of bovine viral diarrhoea virus (BVDV) isolates obtained from 41 serum samples of persistently infected cattle in the Czech Republic in the period of 2004 to 2007. For the differentiation of BVDV isolates, the 5’-UTR and Npro</sup> regions were selected. A 288-bp fragment from 5’-UTR and 428-bp fragment from Npro of the selected isolates were amplified by RT-PCR and subsequently sequenced and analysed by computer-assisted phylogenetic analysis. The isolates belong to BVDV-1 genotype and the following subtypes were identified: b (<i>n</i> = 16), d (<i>n</i> = 16), e (<i>n</i> = 2) and f (<i>n</i> = 7). In this collection of viral samples, no isolate belonged to BVDV-2 genotype.

scholarly journals Evaluation of diagnostic methods to distinguish between calves persistently and transiently infected with bovine viral diarrhoea virus in respect to the presence of maternal antibodies

2013 ◽  
Vol 57 (3) ◽  
pp. 311-317
Author(s):  
Magdalena Larska ◽  
Aleksandra Kuta ◽  
Mirosław P. Polak
Keyword(s):  
Virus Detection ◽  
Bovine Viral Diarrhoea Virus ◽  
Diagnostic Methods ◽  
Viral Rna ◽  
Maternal Antibodies ◽  
Bovine Viral Diarrhoea ◽  
Rt Pcr ◽  
Serum Samples ◽  
Virus Genotype ◽  
Diarrhoea Virus

Abstract Two issues concerning virus detection and identification of persistently infected (PI) cattle were analysed in the study: 1) interference by maternal antibodies and 2) discrimination between PI and transiently infected (TI) animals. Antigen ELISA and RT-PCR based methods were compared using serum samples from natural and experimental PI and TI calves. RT-PCR and realtime RT-PCR using primers within 5’UTR region were more sensitive in detecting PI animals than Erns and NS3 antigen capture ELISAs, and they were not influenced by the presence of colostral antibodies in serum or by bovine viral diarrhoea virus genotype. The serum samples with Ct values ≤ 29.10 (corresponding to 104.87 viral RNA copies/μL) identified PI animals with 100% probability, while all samples with Ct values > 32.06 (corresponding to viral RNA load below 104 copies/μL) indicated TI status. The samples with Ct values between 29.10 and 32.06 (17.2% of PI and 11.5% of TI) should be considered as PI suspect and retested.

scholarly journals Serological survey of bovine viral diarrhoea virus in Namibian and South African kudu (Tragelaphus strepsiceros) and eland (Taurotragus oryx)

2013 ◽  
Vol 84 (1) ◽  
Author(s):  
Terence P. Scott ◽  
Eleanor Stylianides ◽  
Wanda Markotter ◽  
Louis Nel
Keyword(s):  
South Africa ◽  
South African ◽  
Southern Africa ◽  
Bovine Viral Diarrhoea Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bovine Viral Diarrhoea ◽  
Serum Samples ◽  
Diarrhoea Virus ◽  
Central Regions ◽  
Tragelaphus Strepsiceros

Bovine viral diarrhoea virus (BVDV) is a pestivirus that affects members of the order Artiodactyla, including members of the subfamily Bovinae. Little is known about the seroprevalence of BVDV in southern Africa, especially the prevalence in wild ruminant populations such as kudu (Tragelaphus strepsiceros). A handful of random surveys suggested that seroprevalence ranged between 6% and 70% in southern African wild ruminants. The present study aimed to determine the seroprevalence of BVDV amongst kudu and eland (Taurotragus oryx) from Namibia and South Africa. A BVDV-specific enzyme-linked immunosorbent assay was performed on 50 serum samples from kudu and eland from South Africa and Namibia. The seroprevalence of BVDV in South African kudu was 71%, identical to that in Namibian kudu. The seroprevalence in Namibian eland was 40%. The kudu and cattle farming (free ranging) regions in Namibia predominantly overlap in the central regions, ensuring ample opportunity for cross-species transmission of BVDV. It is therefore important to determine the true prevalence of BVDV in southern Africa in both domesticated and wild animals. In addition, a potential link between BVDV incidence and a devastating rabies epidemic in Namibian kudu was proposed and such a notion could be supported or discredited by comparative prevalence data.

Storage of bovine viral diarrhoea virus samples on filter paper and detection of viral RNA by a RT-PCR method

2001 ◽  
Vol 92 (1) ◽  
pp. 19-22 ◽  
Author(s):  
Štefan Vilček ◽  
Ladislav Strojny ◽  
Branislav Ďurkovič ◽  
Wigbert Rossmanith ◽  
David Paton
Keyword(s):  
Filter Paper ◽  
Bovine Viral Diarrhoea Virus ◽  
Viral Rna ◽  
Bovine Viral Diarrhoea ◽  
Rt Pcr ◽  
Diarrhoea Virus ◽  
Pcr Method

Effects of bovine viral diarrhoea virus on the fertility of cows

2013 ◽  
Vol 61 (2) ◽  
pp. 281-289 ◽  
Author(s):  
Sibel Yavru ◽  
Mehmet Kale ◽  
Mehmet Gulay ◽  
Orhan Yapici ◽  
Oya Bulut ◽  
...  
Keyword(s):  
Bovine Viral Diarrhoea Virus ◽  
Bovine Viral Diarrhoea ◽  
Rt Pcr ◽  
Blood Samples ◽  
Frozen Semen ◽  
Diarrhoea Virus ◽  
Mucous Discharge ◽  
Service Conception ◽  
Negative Effect ◽  
Polymerase Chain

The aim of the present study was to determine the possible relationship between bovine viral diarrhoea (BVD) virus infection and the appearance of cervical mucous discharge (CMD) and the reproductive performance of cows in oestrus. For this purpose, CMD from 97 Holstein cows in oestrus was evaluated visually before artificial insemination (AI). Cows in oestrus were inseminated with frozen semen free from BVD virus (BVDV). Blood samples were tested by enzyme-linked immunoassay (ELISA) for antigen (Ag) and antibodies (Ab) of BVDV. The presence of the BVDV genome in cervical mucus samples was tested by reverse transcriptase-polymerase chain reaction (RT-PCR). The presence of BVDV Ab, Ag or genome was not associated with abnormal cervical mucous discharge (A-CMD). However, the presence of BVDV Ag (but not of the BVDV Ab) in blood samples was associated with a lower first service conception rate (FSCR; 27.8 vs. 70.9%; P < 0.01), indicating that BVDV viraemia at the time of AI has a negative effect on the fertility of cows.

scholarly journals Genetic typing of bovine viral diarrhoea virus in cattle on Irish farms

2017 ◽  
Vol 111 ◽  
pp. 14-20 ◽  
Author(s):  
Eoin O'Brien ◽  
Marie Garvey ◽  
Cathal Walsh ◽  
Sean Arkins ◽  
Ann Cullinane
Keyword(s):  
Bovine Viral Diarrhoea Virus ◽  
Bovine Viral Diarrhoea ◽  
Genetic Typing ◽  
Diarrhoea Virus

Genetic typing of bovine viral diarrhoea virus: most Slovenian isolates are of genotypes 1d and 1f

2004 ◽  
Vol 99 (3-4) ◽  
pp. 175-185 ◽  
Author(s):  
Ivan Toplak ◽  
Torstein Sandvik ◽  
Darja Barlič-Maganja ◽  
Jože Grom ◽  
David J Paton
Keyword(s):  
Bovine Viral Diarrhoea Virus ◽  
Bovine Viral Diarrhoea ◽  
Genetic Typing ◽  
Diarrhoea Virus

Direct RT-PCR from serum enables fast and cost-effective phylogenetic analysis of bovine viral diarrhoea virus

2013 ◽  
Vol 190 (1-2) ◽  
pp. 1-3 ◽  
Author(s):  
Claudia Bachofen ◽  
Kim Willoughby ◽  
Ruth Zadoks ◽  
Paul Burr ◽  
Dominic Mellor ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Bovine Viral Diarrhoea Virus ◽  
Cost Effective ◽  
Bovine Viral Diarrhoea ◽  
Rt Pcr ◽  
Diarrhoea Virus

scholarly journals Serological survey of British sheep flocks for evidence of exposure to ovine pestiviruses

2018 ◽  
Vol 183 (7) ◽  
pp. 220-220 ◽  
Author(s):  
Amy Jennings ◽  
Emily Gascoigne ◽  
Alastair Macrae ◽  
Elizabeth Burrough ◽  
James Patrick Crilly
Keyword(s):  
Great Britain ◽  
Metabolic Profiling ◽  
Bovine Viral Diarrhoea Virus ◽  
Border Disease Virus ◽  
Serological Survey ◽  
Bovine Viral Diarrhoea ◽  
Sheep Flock ◽  
Seropositive Animal ◽  
Border Disease ◽  
Diarrhoea Virus

Ovine pestiviruses have the potential to reduce productivity in the British sheep flock. However, their prevalence and impact are currently poorly understood. This study aimed to estimate the exposure to pestiviruses in adult breeding ewe stock. Blood samples collected for metabolic profiling before lambing were tested using an ELISA that detected antibodies raised to both bovine viral diarrhoea virus and Border disease virus. A group of 15 animals were tested per flock. A total of 34 farms were tested, of which 13 had at least one seropositive animal. In those positive flocks between one and nine of the animals tested antibody-positive. Positive flocks were identified in all regions of Great Britain. This work suggests that exposure to ovine pestiviruses is widespread, and that it is timely to investigate flock-level prevalence and possible production impacts of endemic infection.

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