Characterization of indica-type giant embryo mutant rice enriched with nutritional components

There are a number of bioactive compounds in freshwater fish brains, and their functional roles have not been clearly elucidated. NMR-based metabolic profiling could provide a rapid characterization of a fish brain's nutritional composition.

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Molecular cloning and characterization of glutamate decarboxylase cDNA from the giant-embryo Oryza sativa

Archives of Biological Sciences ◽

10.2298/abs1004873x ◽

2010 ◽

Vol 62 (4) ◽

pp. 873-879 ◽

Cited By ~ 3

Author(s):

Hui Xu ◽

Pan Liao ◽

Jianbo Xiao ◽

Qianfei Zhang ◽

Yanjun Dong ◽

...

Keyword(s):

Oryza Sativa ◽

Glutamate Decarboxylase ◽

Structural Features ◽

Full Length ◽

Gamma Aminobutyric Acid ◽

Full Length Cdna ◽

Gaba Production ◽

Calculated Molecular Weight ◽

Giant Embryo

A full-length cDNA encoding glutamate decarboxylase (designated as OsGAD3), which catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA), was isolated from the GABA-rich giant-embryo Oryza sativa (Shangshi Jing 315). The full-length cDNA of OsGAD3 (SSJ315) has a 1479 bp open reading frame (ORF) encoding a protein of 492 amino acid residues. The deduced protein had an isoelectric point (pI) of 5.72 and a calculated molecular weight of 56.1 kD. Sequence comparison showed that OsGAD3 (SSJ315) matches the glutamate decarboxylases of other plant species reported previously. Analysis of the structural features of the C-terminal portions of plant GADs revealed that OsGAD3 (SSJ315) has the typical CaM-binding domain (CaMBD) in the C-terminal region as most other plant GADs. Evolution analysis showed that plant GADs are conserved in the process of evolution. The cloning and characterization of the OsGAD3 (SSJ315) gene will enable us to use OsGAD3 to enhance GABA production in O. sativa (SSJ315) by metabolic engineering in the near future.

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Characterization of Reciprocal Hybrids of Common Ragweed,Ambrosia artemisiifolia, and Giant Ragweed,A. trifida

Weed Science ◽

10.1017/s0043174500075421 ◽

1988 ◽

Vol 36 (5) ◽

pp. 574-576 ◽

Cited By ~ 2

Author(s):

Gilles Vincent ◽

Denis Lauzer ◽

Mario Cappadocia

Keyword(s):

Leaf Area ◽

Leaf Morphology ◽

Immature Embryo ◽

Ambrosia Artemisiifolia ◽

Common Ragweed ◽

Reciprocal Hybrids ◽

Giant Ragweed ◽

Immature Embryo Culture ◽

Type Giant

Common ragweed (2n=36), giant ragweed (2n = 24), and their reciprocal F1hybrids (2n = 30) were analyzed for a number of traits. Hybrids of the type giant ragweed♀ × common ragweed♂ were obtained via immature embryo culture. The analyses revealed a surprising contribution of the pollen parent for features such as biomass, leaf area, and specific leaf area. Leaf morphology, however, was similar to the maternal parent. One plant, originating from crosses of the type common ragweed♀ × giant ragweed♂, developed abnormally and cytological analysis revealed the presence of 24 chromosomes in its somatic cells. Probably this plant had an androgenetic origin.

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