Influence of Postemergence Dicamba/Glyphosate Timing and Inclusion of Acetochlor as a Layered Residual on Weed Control and Soybean Yield

Roundup Ready 2 Xtend® [glyphosate- and dicamba-resistant (DR)] soybean is a novel trait option for postemergence (POST) control of herbicide-resistant broadleaf weeds in soybean. With increased use of labeled dicamba products POST in DR soybean and recommendations to include a soil-residual herbicide POST (e.g., layered residual approach), research on how combinations of these approaches influence weed control, weed seed production, and soybean grain yield is warranted. The objective of this research was to evaluate the effects of (1) flumioxazin applied preemergence (PRE) followed by (fb) dicamba plus glyphosate applied POST at different crop developmental stages and (2) acetochlor POST as a layered residual approach on weed control, weed seed production, and soybean yield to determine the optimal POST timing in DR soybean. A field study was conducted in Wisconsin at three sites in 2018 and four sites in 2019 to evaluate flumioxazin (43.4 g ai ha−1, WDG 51%) PRE fb dicamba (560 g ae ha−1, SL) plus glyphosate (1,101 g ae ha−1, SL) POST in DR soybean at three stages: early-POST (EPOST, V1-V2), mid-POST (MPOST, V3-V4), and late-POST (LPOST, V5-V6/R1) with or without a soil-residual herbicide POST (acetochlor, 1,262 g ai ha−1, ME). Weed community composition was site-specific; difficult-to-control broadleaf species included giant ragweed (Ambrosia trifida L.) and waterhemp [Amaranthus tuberculatus (Moq.) J.D. Sauer]. Dicamba plus glyphosate applied MPOST and LPOST provided greater control, weed biomass reduction, and density reduction of giant ragweed and waterhemp when compared with EPOST treatments. Giant ragweed and waterhemp had not reached 100% cumulative emergence at EPOST, and plants that emerged after EPOST produced seed. There was some benefit to including acetochlor as a layered residual at EPOST as indicated by a residual by POST timing interaction for waterhemp density reduction. Complete waterhemp control was not attained at one site-year. For remaining site-years, dicamba plus glyphosate applied MPOST (V3-V4) provided season-long weed control, reduced weed seed production, and optimized soybean grain yield compared with other POST treatments. Results highlight the importance of timely POST applications and suggest utilization of a POST layered residual needs to be timed appropriately for the window of active weed species emergence.

Utilizing Cover Crops for Weed Suppression within Buffer Areas of 2,4-D-Resistant Soybean

Cover crops can be utilized to suppress weeds via direct competition for sunlight, water, and soil nutrients. Research was conducted to determine if cover crops can be used in label mandated buffer areas in 2,4-D-resistant soybean cropping systems. Delaying termination of cover crops containing cereal rye to at, or after, soybean planting resulted in a 25 to over 200 percentage point increase in cover crop biomass compared to a control treatment. Cover crops generally improved horseweed control when 2,4-D was not used. Cover crops reduced grass densities up to 54% at four of six site-years when termination was delayed to after soybean planting. Cover crops did not reduce giant ragweed densities. Cover crops reduced waterhemp densities by up to 45%. Cover crops terminated at, or after planting were beneficial within buffer areas for control of grassess and waterhemp, but not giant ragweed. Yield reductions of 14 to 41% occurred when cover crop termination was delayed to after soybean planting at three of six site-years. Terminating the cover crops at planting time provided suppression of grasses and waterhemp within buffer areas and had similar yield to the highest yielding treatment in five out of six site-years.

Comparative analyses of transcriptional responses of Dectes texanus LeConte (Coleoptera: Cerambycidae) larvae fed on three different host plants and artificial diet

Dectes texanus is an important coleopteran pest of soybeans and cultivated sunflowers in the Midwestern United States that causes yield losses by girdling stems of their host plants. Although sunflower and giant ragweed are primary hosts of D. texanus, they began colonizing soybeans approximately 50 years ago and no reliable management method has been established to prevent or reduce losses by this pest. To identify genes putatively involved when feeding soybean, we compared gene expression of D. texanus third-instar larvae fed soybean to those fed sunflower, giant ragweed, or artificial diet. Dectes texanus larvae differentially expressed 514 unigenes when fed on soybean compared to those fed the other diet treatments. Enrichment analyses of gene ontology terms from up-regulated unigenes in soybean-fed larvae compared to those fed both primary hosts highlighted unigenes involved in oxidoreductase and polygalacturonase activities. Cytochrome P450s, carboxylesterases, major facilitator superfamily transporters, lipocalins, apolipoproteins, glycoside hydrolases 1 and 28, and lytic monooxygenases were among the most commonly up-regulated unigenes in soybean-fed larvae compared to those fed their primary hosts. These results suggest that D. texanus larvae differentially expressed unigenes involved in biotransformation of allelochemicals, digestion of plant cell walls and transport of small solutes and lipids when feeding in soybean.

The Changes of Soil Seed Banks In Time Series Before And After The Invasive Artemisia Trifida Was Removed From Grassland

Artemisia trifida (giant ragweed) is an invasive weed with an expanding distribution area. In recent years it has been found to invade grasslands, bringing great challenges for effective weed control and restoration of native herbage. Although it has been reported that plant invasion can cause a decline in species richness and biodiversity in native seed banks, which may eventually lead to the depletion of native seed banks, few location- and species-specific case studies have been conducted regarding the dynamic characteristics of the invaded seed banks from invasion back to restoration. The purpose of this study was to compare and quantify the seed banks of grassland communities after (1) giant ragweed invasion for 0-8 years, and (2) giant ragweed removal, in Yili Valley, Xinjiang, China. The results showed that the duration of invasion determined whether giant ragweed could pose a significant threat to the native community seed bank. The seed bank density of native community had significantly decreased by 30.44% after 4 years of invasion, and in the sixth year, the species richness in the seed bank had decreased significantly by 12.36%. After the invasion had lasted for eight years, the seed bank density of the native community had decreased by 83.28%, the species richness in the seed bank decreased by 39.33%, and the seed bank tended to be homogeneous. After the giant ragweed was removed, the potential for restoration was limited by the residual seed bank. Three years after the restoration, although the density of seed banks increased significantly, new growth was dominated by weedy species, rather than crucial components of the grassland habitat. This study is of great significance to the control of giant ragweed and the restoration of grassland vegetation invaded by giant ragweed.

Giant ragweed (Ambrosia trifida L.) in the territory of the Vladimir region

Relevance. The article provides data on the distribution of Ambrosia trifida L. in the Vladimir region, beyond its borders in Russia and in the world as a whole. A new place of growth of Ambrosia trifida L. was revealed in the city of Vladimir. The use of ragweed as a medicinal plant and its role in human life are described. Taking into account the fact that ragweed damages agriculture and is included in the list of quarantine objects, the work provides general and specific measures to combat it.

Ambrosia trifida (giant ragweed).

A. trifida is an annual herb native to temperate North America which is now present in a number of countries in Europe and Asia. The primary means of spread of A. trifida occurs accidentally as a contaminant of seed or agricultural equipment. This species readily colonises disturbed areas and is often one of the first plants to emerge in early spring. As a result it has an initial competitive advantage and can therefore behave as a dominant species throughout the entire growing season. A. trifida is a particular problem for cultivated agricultural and horticultural crops where it can significantly decrease yields. Like many species of Ambrosia, A. trifida produces pollen which is allergenic and can induce allergic rhinitis, fever, or dermatitis. A. trifida is extremely competitive and can also decrease native biodiversity. A. trifida is a declared noxious weed in its native range in California, Delaware, Illinois and New Jersey, USA and is also a quarantine weed in Poland and Russia.

Ambrosia trifida L.: Giant ragweed

Ambrosia trifida L. (AMBTR, fam. Asteraceae/Compositae) is native to North America. It was introduced accidentally to Europe at the end of the 19th century, with contaminated animal feed and seeds for planting. Today A. trifida is present in ruderal and agricultural habitats of many European countries (France, Italy, Germany, Russia, Spain, Romania, Slovakia, Czech Republic, Poland, Serbia, Bulgaria, etc.). Giant ragweed was detected for the first time in 1981 in Serbia (site Čoka). Over the following period it disappeared from this site, but was recorded again in 2006 in another site (central Bačka: Despotovo, Kucura, Savino Selo, Ravno Selo, Ruski Krstur). Currently in Serbia it has the status of an alien naturalized weed species. This summer annual plant can grow up to 6 m in height and exhibits a high degree of morphological and reproductive plasticity in response to encroachment by neighboring plants. It is present in disturbed habitats, such as agriculture fields, where it plays the role of the dominant species throughout the entire growing season. In most cases, leaves are opposite and always simple and generally have 3 distinct lobes but can also have as many as 5. It is a diploid (2n = 24), meso-hygrophilic species, preferring wet habitatse and can tolerate a wide variety of soil types. Also, this is a monoecious plant, where male and female flowers are separated on the same individual. A. trifida can hybridise with A. artemisiifolia (A. x helenae Rouleau, with 2n= 27 and 2n= 33), but this hybrid has been described as sterile. Compared to other summer annual species, A. trifida is among the first to emerge in early spring, at optimal temperatures from 10-24°C. Under optimal environmental conditions, giant ragweed produces around 1,800 (max 5,100) seeds plant-1. It flowers and bears fruit from July to September (October).The pollen of this species has allergenic potential. Additionally, in the USA and Canada giant ragweed populations have developed resistance to acetolactate synthase inhibitor herbicides and glyphosate. Giant ragweed can be a problematic weed in row crops (corn, soybean, sunflower, sugerbeet) and vegetables. In A. trifida the control measures should prevent further spread, and existing populations should be controlled by integrated weed management practices. Furthermore, A. trifida has a relatively low fecundity, a transient soil seedbank and a high percentage of non-viable or low-survivorship seeds, which are features that may have constrained its establishment and spread in the current environmental conditions in Serbia.

Control of glyphosate-resistant horseweed and giant ragweed in soybean with halauxifen-methyl applied preplant

Horseweed and giant ragweed are competitive, annual weeds that can negatively impact crop yield. Biotypes of glyphosate-resistant (GR) giant ragweed and horseweed were first reported in 2008 and 2010 in Ontario, respectively. GR horseweed has spread throughout the southern portion of the province. The presence of GR biotypes poses new challenges for soybean producers in Canada and the United States. Halauxifen-methyl is a recently registered selective herbicide for broadleaf weeds, for preplant use in corn and soybean. There is limited literature on the efficacy of halauxifen-methyl on GR horseweed and giant ragweed when combined with currently registered products in Canada. The purpose of the experiment was to determine the effectiveness of halauxifen-methyl applied alone, and tank-mixed for GR giant ragweed and GR horseweed control in glyphosate and dicamba-resistant (GDR) soybean in southwestern Ontario. Six field experiments were conducted separately for each weed species over 2018 and 2019. Halauxifen-methyl applied alone controlled GR horseweed 72% at 8 weeks after application (WAA). Control was improved to >91% when halauxifen-methyl applied in combination with metribuzin, saflufenacil, chlorimuron-ethyl + metribuzin and saflufenacil + metribuzin. At 8 WAA, halauxifen-methyl controlled GR giant ragweed 11%; glyphosate/2,4-D choline, glyphosate/dicamba, glyphosate/2,4-D choline + halauxifen-methyl and glyphosate/dicamba + halauxifen-methyl controlled GR giant ragweed 76 to 88%. This study concluded that halauxifen-methyl applied preplant in a tank-mixture can provide effective control of GR giant ragweed and horseweed in GDR soybean.

Pollen-mediated gene flow and transfer of resistance alleles from herbicide-resistant broadleaf weeds

Pollen-mediated gene flow (PMGF) refers to the transfer of genetic information (alleles) from one plant to another compatible plant. With the evolution of herbicide-resistant (HR) weeds, PMGF plays an important role in the transfer of resistance alleles from HR to susceptible weeds; however, little attention is given to this topic. The objective of this work was to review reproductive biology, PMGF studies, and interspecific hybridization, as well as potential for herbicide resistance alleles to transfer in the economically important broadleaf weeds including common lambsquarters, giant ragweed, horseweed, kochia, Palmer amaranth, and waterhemp. The PMGF studies involving these species reveal that transfer of herbicide resistance alleles routinely occurs under field conditions and is influenced by several factors, such as reproductive biology, environment, and production practices. Interspecific hybridization studies within Amaranthus and Ambrosia spp. show that herbicide resistance allele transfer is possible between species of the same genus but at relatively low levels. The widespread occurrence of HR weed populations and high genetic diversity is at least partly due to PMGF, particularly in dioecious species such as Palmer amaranth and waterhemp compared with monoecious species such as common lambsquarters and horseweed. Prolific pollen production in giant ragweed contributes to PMGF. Kochia, a wind-pollinated species can efficiently disseminate herbicide resistance alleles via both PMGF and tumbleweed seed dispersal, resulting in widespread occurrence of multiple HR kochia populations. The findings from this review verify that intra- and interspecific gene flow can occur and, even at a low rate, could contribute to the rapid spread of herbicide resistance alleles. More research is needed to determine the role of PMGF in transferring multiple herbicide resistance alleles at the landscape level.

Effect of cereal rye and canola on winter and summer annual weed emergence in corn

Field experiments were conducted in 2017 and 2018 at two locations in Indiana to evaluate the influence of cover crop species, termination timing, and herbicide treatment on winter and summer annual weed suppression and corn yield. Cereal rye and canola cover crops were terminated early or late (2 wk before or after corn planting) with a glyphosate- or glufosinate-based herbicide program. Canola and cereal rye reduced total weed biomass collected at termination by up to 74% and 91%, in comparison to fallow, respectively. Canola reduced horseweed density by up to 56% at termination and 57% at POST application compared to fallow. Cereal rye reduced horseweed density by up to 59% at termination and 87% at POST application compared to fallow. Canola did not reduce giant ragweed density at termination in comparison to fallow. Cereal rye reduced giant ragweed density by up to 66% at termination and 62% at POST application. Termination timing had little to no effect on weed biomass and density reduction in comparison to the effect of cover crop species. Cereal rye reduced corn grain yield at both locations in comparison to fallow, especially for the late-termination timing. Corn grain yield reduction up to 49% (4,770 kg ha–1) was recorded for cereal rye terminated late in comparison to fallow terminated late. Canola did not reduce corn grain yield in comparison to fallow within termination timing; however, late-terminated canola reduced corn grain yield by up to 21% (2,980 kg ha–1) in comparison to early-terminated fallow. Cereal rye can suppress giant ragweed emergence, whereas canola is not as effective at suppressing large-seeded broadleaves such as giant ragweed. These results also indicate that early-terminated cover crops can often result in higher corn grain yields than late-terminated cover crops in an integrated weed management program.