Development of two powdery mildew and stripe rust resistant wheat lines from (Triticum turgidum×Haynaldia villosaamphiploid) × synthetic wheat hybrids

2010 ◽  
Vol 38 (3) ◽  
pp. 307-316 ◽  
Author(s):  
X. Li ◽  
H. Liu ◽  
J. Gao ◽  
H. Wang
Keyword(s):  
Powdery Mildew ◽  
Stripe Rust ◽  
Triticum Turgidum ◽  
Synthetic Wheat ◽  
Wheat Hybrids ◽  
Wheat Lines

scholarly journals Production of synthetic wheat lines to exploit the genetic diversity of emmer wheat and D genome containing Aegilops species in wheat breeding

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ghader Mirzaghaderi ◽  
Zinat Abdolmalaki ◽  
Rahman Ebrahimzadegan ◽  
Farshid Bahmani ◽  
Fatemeh Orooji ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Bread Wheat ◽  
Triticum Turgidum ◽  
Wheat Breeding ◽  
Emmer Wheat ◽  
Synthetic Wheat ◽  
D Genome ◽  
Synthetic Hexaploid ◽  
Wheat Lines

AbstractDue to the accumulation of various useful traits over evolutionary time, emmer wheat (Triticum turgidum subsp. dicoccum and dicoccoides, 2n = 4x = 28; AABB), durum wheat (T. turgidum subsp. durum, 2n = 4x = 28; AABB), T. timopheevii (2n = 4x = 28; AAGG) and D genome containing Aegilops species offer excellent sources of novel variation for the improvement of bread wheat (T. aestivum L., AABBDD). Here, we made 192 different cross combinations between diverse genotypes of wheat and Aegilops species including emmer wheat × Ae. tauschii (2n = DD or DDDD), durum wheat × Ae. tauschii, T. timopheevii × Ae. tauschii, Ae. crassa × durum wheat, Ae. cylindrica × durum wheat and Ae. ventricosa × durum wheat in the field over three successive years. We successfully recovered 56 different synthetic hexaploid and octaploid F2 lines with AABBDD, AABBDDDD, AAGGDD, D1D1XcrXcrAABB, DcDcCcCcAABB and DvDvNvNvAABB genomes via in vitro rescue of F1 embryos and spontaneous production of F2 seeds on the Fl plants. Cytogenetic analysis of F2 lines showed that the produced synthetic wheat lines were generally promising stable amphiploids. Contribution of D genome bearing Aegilops and the less-investigated emmer wheat genotypes as parents in the crosses resulted in synthetic amphiploids which are a valuable resource for bread wheat breeding.

scholarly journals Development of a new wheat microarray from a durum wheat totipotent cDNA library used for a powdery mildew resistance study

2013 ◽  
Vol 18 (2) ◽  
Author(s):  
Rosa Cifarelli ◽  
Olimpia D’Onofrio ◽  
Rosalba Grillo ◽  
Teresa Mango ◽  
Francesco Cellini ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Durum Wheat ◽  
Cdna Library ◽  
Triticum Turgidum ◽  
Cdna Libraries ◽  
Chromosome 5B ◽  
A Genome ◽  
Gene Expression Studies ◽  
Wheat Lines ◽  
Six Genes

AbstractTotipotent cDNA libraries representative of all the potentially expressed sequences in a genome would be of great benefit to gene expression studies. Here, we report on an innovative method for creating such a library for durum wheat (Triticum turgidum L. var. durum) and its application for gene discovery. The use of suitable quantities of 5-azacytidine during the germination phase induced the demethylation of total DNA, and the resulting seedlings potentially express all of the genes present in the genome. A new wheat microarray consisting of 4925 unigenes was developed from the totipotent cDNA library and used to screen for genes that may contribute to differences in the disease resistance of two near-isogenic lines, the durum wheat cultivar Latino and the line 5BIL-42, which are respectively susceptible and resistant to powdery mildew. Fluorescently labeled cDNA was prepared from the RNA of seedlings of the two near-isogenic wheat lines after infection with a single powdery mildew isolate under controlled conditions in the greenhouse. Hybridization to the microarray identified six genes that were differently expressed in the two lines. Four of the sequences could be assigned putative functions based on their similarity to known genes in public databases. Physical mapping of the six genes localized them to two regions of the genome: the centromeric region of chromosome 5B, where the Pm36 resistance gene was previously localized, and chromosome 6B.

Registration of the Novel Wheat Lines L658, L693, L696, and L699, with Resistance to Fusarium Head Blight, Stripe Rust, and Powdery Mildew

2014 ◽  
Vol 9 (1) ◽  
pp. 121-124 ◽  
Author(s):  
Zhaohui Liu ◽  
Meng Xu ◽  
Zepan Xiang ◽  
Xin Li ◽  
Wanquan Chen ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Fusarium Head Blight ◽  
Stripe Rust ◽  
The Novel ◽  
Head Blight ◽  
Wheat Lines

Chromosomal location of genes for resistance to powdery mildew in Chinese wheat lines Jieyan 94-1-1 and Siyan 94-1-2

Hereditas ◽  
2002 ◽  
Vol 136 (3) ◽  
pp. 212-218 ◽  
Author(s):  
XIU QIANG HUANG ◽  
SAI L. K. HSAM ◽  
FRIEDRICH J. ZELLER
Keyword(s):  
Powdery Mildew ◽  
Chromosomal Location ◽  
Wheat Lines ◽  
Chinese Wheat

Resistance of Tangmai 4 wheat to powdery mildew, stem rust, leaf rust, and stripe rust and its chromosome composition

2004 ◽  
Vol 84 (4) ◽  
pp. 1015-1023 ◽  
Author(s):  
H. J. Li ◽  
R. L. Conner ◽  
B. D. McCallum ◽  
X. M. Chen ◽  
H. Su ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Leaf Rust ◽  
Stripe Rust ◽  
Stem Rust ◽  
Northern China ◽  
Western Canada ◽  
C Banding ◽  
Rust Diseases ◽  
Hard Red Winter Wheat ◽  
Secale Cereale L

The hard red winter wheat Tangmai 4 did not develop symptoms of infection following inoculation with powdery mildew (Erysiphe graminis DC. f. sp. tritici E. Marchal) isolates from regions of western Canada and northern China. Tangmai 4 exhibited resistance to stem rust (Puccinia graminis Pers. f. sp. tritici Eriks. & Henn.) and leaf rust (P. triticina Eriks.) races from western Canada. This wheat line was resistant to individual stripe rust (P. striiformis Westend. f. sp. tritici Eriks.) races from the U.S. and Canada. Sequential C-banding and genomic in situ hybridization (GISH), and electrophoretic analyses of high molecular weight glutenins and gliadins demonstrated that Tangmai 4 carried a pair of T1BL·1RS wheat-rye (Secale cereale L.) translocated chromosomes. Since the genes located on T1BL·1RS are no longer effective in controlling powdery mildew and the rust diseases, Tangmai 4 must carry additional genes for resistance to these diseases, which makes it a valuable resource for the improvement of resistance in wheat against these diseases. Key words: T1BL·1RS translocation, disease resistance, sequential C-banding and GISH, glutenin, gliadin

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