scholarly journals Development of a new wheat microarray from a durum wheat totipotent cDNA library used for a powdery mildew resistance study

2013 ◽  
Vol 18 (2) ◽  
Author(s):  
Rosa Cifarelli ◽  
Olimpia D’Onofrio ◽  
Rosalba Grillo ◽  
Teresa Mango ◽  
Francesco Cellini ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Durum Wheat ◽  
Cdna Library ◽  
Triticum Turgidum ◽  
Cdna Libraries ◽  
Chromosome 5B ◽  
A Genome ◽  
Gene Expression Studies ◽  
Wheat Lines ◽  
Six Genes

AbstractTotipotent cDNA libraries representative of all the potentially expressed sequences in a genome would be of great benefit to gene expression studies. Here, we report on an innovative method for creating such a library for durum wheat (Triticum turgidum L. var. durum) and its application for gene discovery. The use of suitable quantities of 5-azacytidine during the germination phase induced the demethylation of total DNA, and the resulting seedlings potentially express all of the genes present in the genome. A new wheat microarray consisting of 4925 unigenes was developed from the totipotent cDNA library and used to screen for genes that may contribute to differences in the disease resistance of two near-isogenic lines, the durum wheat cultivar Latino and the line 5BIL-42, which are respectively susceptible and resistant to powdery mildew. Fluorescently labeled cDNA was prepared from the RNA of seedlings of the two near-isogenic wheat lines after infection with a single powdery mildew isolate under controlled conditions in the greenhouse. Hybridization to the microarray identified six genes that were differently expressed in the two lines. Four of the sequences could be assigned putative functions based on their similarity to known genes in public databases. Physical mapping of the six genes localized them to two regions of the genome: the centromeric region of chromosome 5B, where the Pm36 resistance gene was previously localized, and chromosome 6B.

Powdery mildew resistance in some new wheat amphiploids (2n = 6x = 42) derived from A- and S-genome diploid progenitors

2012 ◽  
Vol 10 (3) ◽  
pp. 165-170 ◽  
Author(s):  
Khola Rafique ◽  
Awais Rasheed ◽  
Alvina Gul Kazi ◽  
Hadi Bux ◽  
Farah Naz ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Durum Wheat ◽  
Bread Wheat ◽  
Triticum Turgidum ◽  
Seedling Stage ◽  
Aegilops Speltoides ◽  
Germplasm Screening ◽  
B Genome ◽  
A Genome ◽  
Major Resistance Genes

Triticum urartu possesses the Au genome common to bread wheat. Similarly, Triticum monococcum contains the Am genome, which is closely related to the A-genome donor of bread wheat. Aegilops speltoides of the Sitopsis section has the S genome, which is most similar to the B genome of bread and durum wheat when compared with all other wild grasses. Amphiploids developed through bridge crossing between Am/Au and S-genome diploid resources and elite durum cultivars demonstrate enormous diversity to improve both bread and durum wheat cultivars. We evaluated such A-genome amphiploids (Triticum turgidum × T. urartu and T. turgidum × T. monococcum, 2n = 6x = 42; BBAAAmAm/AuAu) and S-genome amphiploids (T. turgidum × Ae. speltoides, 2n = 6x = 42; AABBSS) along with their durum parents (AABB) for their resistance to powdery mildew (PM) at the seedling stage. The results indicated that 104 accessions (53.6%) of A-genome amphiploids (AABBAmAm/AuAu) were resistant to PM at the seedling stage. Of their 24 durum parents, five (20.83%) were resistant to PM and 16 (66.6%) were moderately tolerant. Similarly, ten (50%) accessions of S-genome amphiploids (BBAASS) possessed seedling PM resistance, suggesting a valuable source of major resistance genes. PM screening of the amphiploids and parental durum lines showed that resistance was contributed either by the diploid progenitors or durum parents, or both. We also observed the suppression of resistance in several cases; for example, resistance in durum wheat was suppressed in respective amphiploids. The results from this germplasm screening will facilitate their utilization to genetically control PM and widen the genetic base of wheat.

scholarly journals Effect of Leaf Rust on Grain Yield and Yield Traits of Durum Wheats with Race-Specific and Slow-Rusting Resistance to Leaf Rust

Plant Disease ◽  
2006 ◽  
Vol 90 (8) ◽  
pp. 1065-1072 ◽  
Author(s):  
S. A. Herrera-Foessel ◽  
R. P. Singh ◽  
J. Huerta-Espino ◽  
J. Crossa ◽  
J. Yuen ◽  
...  
Keyword(s):  
Grain Yield ◽  
Durum Wheat ◽  
Leaf Rust ◽  
Triticum Turgidum ◽  
Specific Resistance ◽  
Phenotypic Diversity ◽  
Yield Traits ◽  
Yield Losses ◽  
Slow Rusting ◽  
Wheat Lines

Leaf rust, caused by Puccinia triticina, is an important disease of durum wheat (Triticum turgidum) in many countries. We compared the effectiveness of different types of resistance in International Maize and Wheat Improvement Center-derived durum wheat germ plasm for protecting grain yield and yield traits. In all, 10 durum wheat lines with race-specific resistance, 18 with slow-rusting resistance, and 2 susceptible were included in two yield loss trials sown on different planting dates in Mexico with and without fungicide protection under high disease pressure. Eight genotypes with race-specific resistance were immune to leaf rust. Durum wheat lines with slow-rusting resistance displayed a range of severity responses indicating phenotypic diversity. Mean yield losses for susceptible, race-specific, and slow-rusting genotypes were 51, 5, and 26%, respectively, in the normal sowing date trial and 71, 11, and 44% when sown late. Yield losses were associated mainly with a reduction in biomass, harvest index, and kernels per square meter. Slow-rusting durum wheat lines with low disease levels and low yield losses, as well as genotypes with low yield losses despite moderate disease levels, were identified. Such genotypes can be used for breeding durum wheat genotypes with higher levels of resistance and negligible yield losses by using strategies that previously have been shown to be successful in bread wheat.

scholarly journals Applicability of chromosome-specific SSR wheat markers for the introgression of Triticum urartu in durum wheat breeding programmes

2011 ◽  
Vol 9 (3) ◽  
pp. 439-444 ◽  
Author(s):  
C. Rodríguez-Suárez ◽  
M. C. Ramírez ◽  
A. Martín ◽  
S. G. Atienza
Keyword(s):  
Durum Wheat ◽  
Triticum Turgidum ◽  
Crop Improvement ◽  
Wheat Chromosome ◽  
Wheat Breeding ◽  
Triticum Urartu ◽  
Founder Line ◽  
A Genome ◽  
Breeding Programmes ◽  
Novel Alleles

Triticum urartu, the A-genome donor of tetraploid and hexaploid wheats, is a potential source of novel alleles for crop improvement. A fertile amphiploid between T. urartu (2n = 2x = 14; AuAu) and durum wheat cv ‘Yavaros’ (Triticum turgidum ssp. durum; 2n = 4x = 28, AABB) was obtained as a first step to making the genetic variability of the wild ancestor available to durum wheat breeding. The amphiploid was backcrossed with ‘Yavaros’ and the offspring from this cross was selfed. A plant from this progeny (founder line) with 28 chromosomes and active x and y subunits of the Glu-A1 locus of T. urartu was selfed, which resulted in the obtaining of 98 pre-introgression lines (pre-ILs). In this work, a set of 78 wheat chromosome-specific microsatellite markers (simple sequence repeats, SSR), uniformly distributed over the A genome, was used for marker-assisted selection of T. urartu in a durum wheat background. A total of 57 SSRs allowed a clear discrimination between T. urartu and ‘Yavaros’. This set of markers was further used for characterizing the pre-ILs, identifying and defining the T. urartu introgressed regions. The applicability of these markers is discussed.

Effect of Zn on the concentration of Cd and Zn in plant tissue of two durum wheat lines

1995 ◽  
Vol 75 (2) ◽  
pp. 445-448 ◽  
Author(s):  
M. Choudhary ◽  
L. D. Bailey ◽  
C. A. Grant ◽  
D. Leisle
Keyword(s):  
Durum Wheat ◽  
Plant Tissue ◽  
Dry Matter ◽  
Triticum Turgidum ◽  
Foliar Application ◽  
Zn Concentration ◽  
P Fertilizer ◽  
Order Root ◽  
Wheat Lines ◽  
P Fertilizers

Irrespective of fertilizer treatments, durum wheat (Triticum turgidum L. var durum) Cd concentration was lowest in the grain and highest in the roots and was in the order: root > leaf > stem > grain. Soil-applied Zn decreased the Cd concentrations in durum wheat grain, leaf, stem, and root. But, foliar application of Zn resulted in Zn loading of the leaf and stem and had little effect on plant Cd concentration. Application of P and N plus P fertilizer increased tissue Cd concentration and decreased stem and leaf Zn concentration to near insufficiency, while application of Zn to soil with N plus P fertilizers increased tissue Zn concentration to sufficiency level and decreased plant Cd concentration of the two durum wheat lines. Key words: Cd, durum wheat, Zn, dry matter, plant tissue

scholarly journals Development and Identification of Four New Synthetic Hexaploid Wheat Lines with Solid Stems

2021 ◽  
Author(s):  
Dongyu Liang ◽  
Minghu Zhang ◽  
Xin Liu ◽  
Hui Li ◽  
Zhenjiao Jia ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Hexaploid Wheat ◽  
Triticum Turgidum ◽  
Chromosome Doubling ◽  
Aegilops Tauschii ◽  
Synthetic Hexaploid Wheat ◽  
Vascular Bundles ◽  
Lodging Resistance ◽  
Synthetic Hexaploid ◽  
Wheat Lines

Abstract Stem solidness is an important agronomic trait for increasing the ability of wheat to resist lodging. In this study, four new synthetic hexaploid wheat with solid stems were developed from natural chromosome doubling of F1 hybrids between a solid-stemmed durum wheat (Triticum turgidum ssp. durum, 2n = 4x = 28, AABB) and four Aegilops tauschii (2n = 2x = 14, DD) accessions. The solid expression of the second internode at the base of the stem was stable for two synthetic hexalpoid wheat Syn-SAU-117 and Syn-SAU-119 grown in both the greenhouse and field. The lodging resistance of four synthetic solid-stem wheats is stronger than that of CS, and Syn-SAU-116 has the strongest lodging resistance, followed by Syn-SAU-119. The paraffin sections of the second internode showed that four synthetic wheat lines had large outer diameters, well-developed mechanical tissues, large number of vascular bundles, and similar anatomical characteristics with solid-stemmed durum wheat. The chromosomal composition of four synthetic hexaploid wheat was identified by FISH (fluorescence in situ hybridization) using Oligo-pSc119.2-1 and Oligo-pTa535-1. At adult stage, all four synthetic hexaploid wheat showed high resistance to mixed physiological races of stripe rust pathogen (CYR31, CYR32, CYR33, CYR34). These synthetic hexaploid wheat lines provide new materials for the improvement of common wheat.

scholarly journals Production of synthetic wheat lines to exploit the genetic diversity of emmer wheat and D genome containing Aegilops species in wheat breeding

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ghader Mirzaghaderi ◽  
Zinat Abdolmalaki ◽  
Rahman Ebrahimzadegan ◽  
Farshid Bahmani ◽  
Fatemeh Orooji ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Bread Wheat ◽  
Triticum Turgidum ◽  
Wheat Breeding ◽  
Emmer Wheat ◽  
Synthetic Wheat ◽  
D Genome ◽  
Synthetic Hexaploid ◽  
Wheat Lines

AbstractDue to the accumulation of various useful traits over evolutionary time, emmer wheat (Triticum turgidum subsp. dicoccum and dicoccoides, 2n = 4x = 28; AABB), durum wheat (T. turgidum subsp. durum, 2n = 4x = 28; AABB), T. timopheevii (2n = 4x = 28; AAGG) and D genome containing Aegilops species offer excellent sources of novel variation for the improvement of bread wheat (T. aestivum L., AABBDD). Here, we made 192 different cross combinations between diverse genotypes of wheat and Aegilops species including emmer wheat × Ae. tauschii (2n = DD or DDDD), durum wheat × Ae. tauschii, T. timopheevii × Ae. tauschii, Ae. crassa × durum wheat, Ae. cylindrica × durum wheat and Ae. ventricosa × durum wheat in the field over three successive years. We successfully recovered 56 different synthetic hexaploid and octaploid F2 lines with AABBDD, AABBDDDD, AAGGDD, D1D1XcrXcrAABB, DcDcCcCcAABB and DvDvNvNvAABB genomes via in vitro rescue of F1 embryos and spontaneous production of F2 seeds on the Fl plants. Cytogenetic analysis of F2 lines showed that the produced synthetic wheat lines were generally promising stable amphiploids. Contribution of D genome bearing Aegilops and the less-investigated emmer wheat genotypes as parents in the crosses resulted in synthetic amphiploids which are a valuable resource for bread wheat breeding.

Karyotypic analysis of two durum wheat varieties

1985 ◽  
Vol 27 (5) ◽  
pp. 617-621 ◽  
Author(s):  
P. J. Bebeli ◽  
P. J. Kaltsikes
Keyword(s):  
Durum Wheat ◽  
Key Words ◽  
Secondary Constriction ◽  
Triticum Turgidum ◽  
Giemsa Banding ◽  
Wheat Varieties ◽  
Karyotypic Analysis ◽  
C Banding ◽  
Chromosome 5B ◽  
Terminal Band

The karyotypes of 'Capeiti' and 'Mexicali', two durum wheat (Triticum turgidum L. var. durum) cultivars were constructed on the basis of the Giemsa banding of their chromosomes. No major differences were noted. Minor differences were as follows: chromosome 4B of 'Mexicali' has more heterochromatin than its counterpart in 'Capeiti', and chromosome 5B in 'Capeiti' has a terminal band on its long arm that is lacking in 'Mexicali', while the opposite is true for the long arm of 6B. In 'Mexicali' 6BS there is a wider band next to the secondary constriction than in 'Capeiti'.Key words: durum wheat, karyotype, C-banding, heterochromatin.

Sign in / Sign up

Export Citation Format

Share Document