Amorphous Silicon Photosensors for Detection of Intrinsic Cell Fluorophores

2011 ◽  
Vol 1321 ◽  
Author(s):  
A. Joskowiak ◽  
V. Chu ◽  
D.M.F. Prazeres ◽  
J.P. Conde
Keyword(s):  
Carbon Content ◽  
Amorphous Silicon ◽  
Nicotinamide Adenine Dinucleotide ◽  
Redox State ◽  
Flavin Adenine Dinucleotide ◽  
Cutoff Wavelength ◽  
Silicon Carbon ◽  
Intrinsic Fluorophores ◽  
Reduced Nicotinamide Adenine Dinucleotide ◽  
High Pass

ABSTRACTAn amorphous silicon (a-Si:H) photoconductor array with two distinct integrated amorphous silicon carbon alloy (a-SiC:H) high pass filters is used to detect two of the cell intrinsic fluorophores. The cutoff wavelength of the filters is tuned by the carbon content in the film. The fluorophores of interest – reduced nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) are indicative of the redox state of the cells. Concentrations down to 1 μM for NADH and 50 μM for FAD were detected.

NAD/NADH: redox state changes on cat brain cortex during stimulation and hypercapnia

1982 ◽  
Vol 243 (4) ◽  
pp. H619-H627 ◽  
Author(s):  
L. Gyulai ◽  
E. Dora ◽  
A. G. Kovach
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Redox State ◽  
Redox System ◽  
Inhibitory Effect ◽  
Nadh Oxidation ◽  
Nicotinamide Adenine ◽  
Direct Inhibitory Effect ◽  
Anterior Suprasylvian Gyrus ◽  
Reduced Nicotinamide Adenine Dinucleotide

The redox state of the anterior suprasylvian gyrus of cats was measured during electrical stimulation and under hypercapnia on cast immobilized and artificially respirated. The state of the nicotinamide adenine dinucleotide-reduced nicotinamide adenine dinucleotide (NAD/NADH) redox system was monitored by in vivo fluorometry. Hypercapnia was produced by inhalation of 10, 15, and 30% CO2, respectively. Hypercapnic acidosis led to NADH oxidation. The NADH oxidation under 30% CO2 inhalation was significantly larger (-14.9 +/- 2.9%) than that observed under 10% (-6.5 +/- 1.9%) and 15% CO2 (-7.0 +/- 1.6%) inhalation. Under normocapnic conditions, stimulation induced NAD reduction to NADH (5.5 +/- 0.8%). The magnitude of the NAD reductive response to stimulation was unaffected by 10% CO2 inhalation, but it was decreased by 15 and 30% CO2 inhalation. The increased concentration of NADH upon stimulation is interpreted as resulting from an increased rate of substrate mobilization. The cause of the oxidation of the NADH pool of the cell during hypercapnia is partly due to the direct inhibitory effect of CO2 on the carbohydrate metabolism, but the role of other mechanisms cannot be neglected either.

Optoelectronic Properties of High-Gap Amorphous Silicon-Carbon Alloys

1995 ◽  
Vol 377 ◽  
Author(s):  
V. Chu ◽  
J. P. Conde ◽  
P. Brogueira ◽  
P. Micaelo ◽  
J. P. Jarego ◽  
...  
Keyword(s):  
Carbon Content ◽  
Amorphous Silicon ◽  
Gas Flow ◽  
Room Temperature ◽  
Deposition Pressure ◽  
Hydrogen Dilution ◽  
Silicon Carbon ◽  
Recombination Centers ◽  
Hydrogenated Amorphous ◽  
Deposition Conditions

ABSTRACTThe dependence of the properties of hydrogenated amorphous silicon-carbon alloys deposited with a 1:10 silane (SiH4) to methane (CH4) or ethylene (C2H4) gas flow ratios with the hydrogen dilution, deposition pressure and power is studied. In methane-based films the carbon content shows a decrease (from ≈0.75 to 0.55) with increasing hydrogen dilution from 0 to 98%, while the ethylene-based films show no dependence of the carbon fraction (≈0.9) on hydrogen dilution. The photoconductivity shows an increase for hydrogen dilutions above 90= for both methane and ethylene-based films. While this increase is attributed, in the case of methane, to the observed decrease in carbon content, no corresponding decrease in carbon content is observed in the ethylene-based films, suggesting a decrease in the density of recombination centers with hydrogen dilution. The Urbach tail and the room-temperature photoluminescence peak correlate with carbon content independent of the carbon source-gas and deposition conditions used.

Considering the Dependence of the Light-Induced Effect on Carbon Content in Boron-Doped Amorphous Silicon-Carbon

1996 ◽  
Vol 35 (Part 1, No. 9A) ◽  
pp. 4626-4627 ◽  
Author(s):  
Masao Isomura ◽  
Makoto Tanaka ◽  
Shinya Tsuda
Keyword(s):  
Carbon Content ◽  
Amorphous Silicon ◽  
Boron Doped ◽  
Silicon Carbon

Artificial cells containing sustainable energy conversion engines

2019 ◽  
Vol 3 (5) ◽  
pp. 573-578 ◽  
Author(s):  
Kwanwoo Shin
Keyword(s):  
Energy Conversion ◽  
Nicotinamide Adenine Dinucleotide ◽  
Sustainable Energy ◽  
Living Cells ◽  
Energy Transduction ◽  
Artificial Cells ◽  
Energy Conversion Process ◽  
Metabolic Reactions ◽  
Metabolic Activities ◽  
Reduced Nicotinamide Adenine Dinucleotide

Living cells naturally maintain a variety of metabolic reactions via energy conversion mechanisms that are coupled to proton transfer across cell membranes, thereby producing energy-rich compounds. Until now, researchers have been unable to maintain continuous biochemical reactions in artificially engineered cells, mainly due to the lack of mechanisms that generate energy-rich resources, such as adenosine triphosphate (ATP) and reduced nicotinamide adenine dinucleotide (NADH). If these metabolic activities in artificial cells are to be sustained, reliable energy transduction strategies must be realized. In this perspective, this article discusses the development of an artificially engineered cell containing a sustainable energy conversion process.

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