Biosurfactant Production Using Mixed Cultures Under Non-Aseptic Conditions

1994 ◽  
Vol 344 ◽  
Author(s):  
C. Vipulanandan ◽  
G. L. Ghurye ◽  
R. C. Willson
Keyword(s):  
Surface Tension ◽  
Yeast Extract ◽  
Substrate Concentration ◽  
Petroleum Hydrocarbons ◽  
Biomass Accumulation ◽  
Biosurfactant Production ◽  
Batch Reactors ◽  
Surface Tension Reduction ◽  
Aseptic Conditions ◽  
Reduced Surface

AbstractSurfactants increase the accessibility of adsorbed hydrocarbons and mobilize immiscible petroleum hydrocarbons for treatment. Biosurfactants have the advantage of biodegradability and non-toxicity over their synthetic counterparts, and can be produced from renewable sources. In this study the production of biosurfactant from molasses was investigated in continuously stirred batch reactors. The effects of substrate concentration, yeast extract and peptone on biomass accumulation and biosurfactant production were investigated. Biosurfactant production was quantified by surface tension reduction and critical micelle dilution (CMD). Biosurfactant production was directly correlated with biomass production, and was improved with the addition of yeast extract. Centrifugation of the whole broth reduced surface tension. The performance of the biosurfactant produced from molasses under non-aseptic condition is comparable to other published results.

scholarly journals Effects of Nitrogen and Carbon Sources on Biosurfactant Production by Hydrocarbon-utilizing Stenotrophomonas sp.

2019 ◽  
pp. 1-10
Author(s):  
Victor Ezebuiro ◽  
Ipeghan Jonathan Otaraku ◽  
Boma Oruwari ◽  
Gideon Chijioke Okpokwasili
Keyword(s):  
Surface Tension ◽  
Olive Oil ◽  
Yeast Extract ◽  
Emulsion Stability ◽  
Carbon Sources ◽  
Glass Slide ◽  
Biosurfactant Production ◽  
Tension Reduction ◽  
Emulsification Index ◽  
Surface Tension Reduction

Aim: This study investigated effects of nitrogen and carbon sources on the production of biosurfactant by a hydrocarbon-utilizing bacterium, Stenotrophomonas sp. Methodology: The hydrocarbon-utilizing bacterium was isolated with Bushnell Haas (BH) broth using enrichment method. Biosurfactant production was screened by evaluating the following characteristics: Emulsification index (E-24), oil spreading (displacement), tilted glass slide, haemolysis on blood agar, and lipase production. Effects of combination of nitrogen sources (yeast extract and NH4NO3, yeast extract and urea, yeast extract and asparagine, yeast extract and peptone, NaNO3 and peptone, NaNO3 and asparagine, and yeast extract and NaNO3) and carbon sources (glucose, fructose, galactose, cassava peel, soya bran, olive oil, sucrose, crude oil, diesel and glycerol) on biosurfactant production were determined with emulsion stability and surface tension as responses. The bacterium was identified based on phenotypic, microscopic, and biochemical characteristics. Results: The isolate produced colonies on BH agar containing either naphthalene or hexadecane as sole source of carbon after 48-h incubation. Screening characteristics for the production of biosurfactant by the isolate were as follows: 46% emulsification index, 3.1 cm2 oil displacement, 1.8 cm zone of clearance on tributyrin agar, γ-haemolysis, and positive tilted glass slide. The best carbon source with the highest emulsion stability (51.6%) was fructose whereas the best surface tension reduction (30.85 mN/m) was observed with olive oil as carbon sources after 7 days of incubation. For nitrogen, the combination of yeast extract and NH4NO3 gave the highest emulsion stability (60.7%) and the best surface tension reduction (39.58 mN/m). The data obtained were significant at P<0.05 and the bacterial isolate identified as Stenotrophomonas sp. Conclusion: This study has demonstrated the ability of the hydrocarbon-utilizing bacterium, Stenotrophomonas sp. to produce biosurfactant, indicated by reduction of surface tension and formation of stable emulsion. This method of biosurfactant production can be further scaled up for industrial purpose. 

scholarly journals UJI PRODUKSI BIOSURFAKTAN OLEH Pseudomonas sp. PADA SUBSTRAT YANG BERBEDA

2007 ◽  
Vol 12 (2) ◽  
pp. 181-185 ◽  
Author(s):  
Fatimah Fatimah
Keyword(s):  
Surface Tension ◽  
Stationary Phase ◽  
Yeast Extract ◽  
Petroleum Hydrocarbon ◽  
Biosurfactant Production ◽  
Lubricating Oil ◽  
Pseudomonas Sp ◽  
Emulsification Activity ◽  
Microbial Metabolite ◽  
Surface Tension Reduction

Biosurfactant, microbial metabolite whose properties like surfactant, was suggested to replace chemically synthesized surfactant for take in hand environtmental pollution by petroleum hydrocarbon. This work was done to examine potency of Pseudomonas sp. isolated from Tanjung Perak Harbor to produce biosurfactant. Also, to know the effect of different substrates (glucose plus yeast extract, lubricating oil and hexadecane) toward biosurfactant production. Pseudomonas sp. grown in mineral synthetic water and biosurfactant production was measured on stationary phase. Biosurfactant production based on emulsification activity and surface tension reduction of supernatant (using Du Nouy tensiometer). Solar, lubricating oil, and hexadecane were used to examine emulsification activity. Results indicated that Pseudomonas sp. have a potency to produce biosurfactant. Surface tension of supernatant decreased up to 20 dyne/cm, when grown on hexadecane substrate. Hexadecane is the best growing substrate for biosurfactant production than others.

scholarly journals Factorial Design to Optimize Biosurfactant Production byYarrowia lipolytica

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
Gizele Cardoso Fontes ◽  
Priscilla Filomena Fonseca Amaral ◽  
Marcio Nele ◽  
Maria Alice Zarur Coelho
Keyword(s):  
Surface Tension ◽  
Factorial Design ◽  
Ammonium Sulfate ◽  
Yeast Extract ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Biosurfactant Production ◽  
Standard Process ◽  
Emulsification Index ◽  
Full Factorial

In order to improve biosurfactant production byYarrowia lipolyticaIMUFRJ 50682, a factorial design was carried out. A24full factorial design was used to investigate the effects of nitrogen sources (urea, ammonium sulfate, yeast extract, and peptone) on maximum variation of surface tension (ΔST) and emulsification index (EI). The best results (67.7% of EI and 20.9 mNm−1ofΔST) were obtained in a medium composed of 10 g 1−1of ammonium sulfate and 0.5 g 1−1of yeast extract. Then, the effects of carbon sources (glycerol, hexadecane, olive oil, and glucose) were evaluated. The most favorable medium for biosurfactant production was composed of both glucose (4% w/v) and glycerol (2% w/v), which provided an EI of 81.3% and aΔST of 19.5 mN m−1. The experimental design optimization enhancedΔEI by 110.7% andΔST by 108.1% in relation to the standard process.

scholarly journals Biosurfactant production by Mucor circinelloides on waste frying oil and possible uses in crude oil remediation

2017 ◽  
Vol 76 (7) ◽  
pp. 1706-1714 ◽  
Author(s):  
Parvin Hasanizadeh ◽  
Hamid Moghimi ◽  
Javad Hamedi
Keyword(s):  
Surface Tension ◽  
Optimum Condition ◽  
Crude Oil ◽  
Fungal Growth ◽  
Mucor Circinelloides ◽  
Frying Oil ◽  
Biosurfactant Production ◽  
Waste Frying Oil ◽  
Tension Reduction ◽  
Surface Tension Reduction

Biosurfactants are biocompatible surface active agents which many microorganisms produce. This study investigated the production of biosurfactants by Mucor circinelloides. The effects of different factors on biosurfactant production, including carbon sources and concentrations, nitrogen sources, and iron (II) concentration, were studied and the optimum condition determined. Finally, the strain's ability to remove the crude oil and its relationship with biosurfactant production was evaluated. The results showed that M. circinelloides could reduce the surface tension of the culture medium to 26.6 mN/m and create a clear zone of 12.9 cm diameter in an oil-spreading test. The maximum surface tension reduction was recorded 3 days after incubation. The optimum condition for biosurfactant production was achieved in the presence of 8% waste frying oil as a carbon source, 2 g/L yeast extract as a nitrogen source, and 0.01 mM FeSO4. M. circinelloides could consume 8% waste frying oil in 5 days of incubation, and 87.6% crude oil in 12 days of incubation. A direct correlation was observed between oil degradation and surface tension reduction in the first 3 days of fungal growth. The results showed that the waste frying oil could be recommended as an inexpensive oily waste substance for biosurfactant production, and M. circinelloides could have the potential to treat waste frying oil. According to the results, the produced crude biosurfactant or fungal strain could be directly used for the mycoremediation of crude oil contamination in oil fields.

scholarly journals The potential of indigenous bacteria from oil sludge for biosurfactant production using hydrolysate of agricultural waste

2019 ◽  
Vol 20 (5) ◽  
Author(s):  
NI’MATUZAHROH NI’MATUZAHROH ◽  
SILVIA KURNIA SARI ◽  
IRINE PUSPA NINGRUM ◽  
APRILLA DILA PUSFITA ◽  
LISA MARJAYANDARI ◽  
...  
Keyword(s):  
Surface Tension ◽  
Raw Materials ◽  
Agricultural Waste ◽  
Hydrolysis Product ◽  
Biosurfactant Production ◽  
Oil Sludge ◽  
Plate Count ◽  
Indigenous Bacteria ◽  
Emulsification Activity ◽  
Surface Tension Reduction

Abstract. Ni’matuzahroh, Sari SK, Ningrum IP, Pusfita AD, Marjayandari L, Trikurniadewi N, Ibrahim SNMM, Fatimah, Nurhariyati T, Surtiningsih T, Yuliani H. 2019. The potential of indigenous bacteria from oil sludge for biosurfactant production using hydrolysate of agricultural waste. Biodiversitas 20: 1374-1379. Biosurfactants are amphipathic compounds which are useful in various fields of health, industry, and remediation. Biosurfactants are produced by bacteria that grow in hydrocarbon or sugar substrates. Hydrolysis product of agricultural waste can be used as a biosurfactant production medium. This research aims to obtain biosurfactant producing bacteria from Balongan oil sludge, Indonesia. The ability to grow and produce biosurfactant by indigenous bacteria was tested using a medium of Synthetic Mineral Water (SMW) added by 209.3 ppm of rice straw hydrolysis product (RSHP). The growth of bacteria was evaluated through Total Plate Count (TPC) and biosurfactant production was evaluated through measurement of emulsification activity and surface tension. Six indigenous bacteria were capable to produce biosurfactants in the RSHP. Emulsification activity was not detected, but surface tension reduction was founded. The best biosurfactant was indicated by surface tension value of 53.56 mN/m with TPC value of 20.07 CFU/mL at the 5th day of incubation by BP (1) 5. The indigenous bacteria were identified as Propionibacterium BP (1) 1, Propionibacterium BP (1) 3, Bacillus BP (1) 4, Corynebacterium BP (1) 5, Corynebacterium BP (1) 8, and Rothia BP (1) 6. Utilization of sugar as hydrolysis product of agricultural waste is an innovation of raw materials for biosurfactant production.

Bioremediation of Petroleum Hydrocarbons Contaminated Soil using Bio piles System

2019 ◽  
Vol 16 (1(Suppl.)) ◽  
pp. 0185
Author(s):  
Jabbar Et al.
Keyword(s):  
Surface Tension ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Bacterial Culture ◽  
Pilot Scale ◽  
Ex Situ ◽  
Total Petroleum Hydrocarbons ◽  
Bacterial Strains ◽  
Mixed Bacterial Culture ◽  
Surface Tension Reduction

This study was focused on biotreatment of soil which polluted by petroleum compounds (Diesel) which caused serious environmental problems. One of the most effective and promising ways to treat diesel-contaminated soil is bioremediation. It is a choice that offers the potential to destroy harmful pollutants using biological activity. Four bacterial strains were isolated from diesel contaminated soil samples. The isolates were identified by the Vitek 2 system, as Sphingomonas paucimobilis, Pentoae species, Staphylococcus aureus, and Enterobacter cloacae. The potential of biological surfactant production was tested using the Sigma 703D stand-alone tensiometer showed that these isolates are biological surfactant producers. The better results of the surface tension reduction test were obtained using the mixed bacterial culture which reduced the surface tension of the medium from 66mN/m to 33.89mN/m. For further evidence of the biodegradation effect of these isolates individually and as a mixed culture, which was supported by the use of Gas-Chromatography technology confirming the occurrence of biodegradation. The capability of mixed bacterial culture was examined to remediate the diesel contaminated soil in bio piles system. Two pilot scale bio piles (25 kg soil each) were constructed containing soils contaminated with approximately 2140 mg/kg total petroleum hydrocarbons (TPHs). Both systems were equipped with oxygen to provide aerobic conditions, incubated at ambient temperature and weekly sampling within 35 days (during summer season). Overall 75.71 % of the total petroleum hydrocarbons were removed from the amended soil and 33.18 % of the control soil at the end of study period. The study concluded that the ex-situ bioremediation (bio piles) is a good option for treating the soil contaminated with diesel as economical and environmentally friendly.

scholarly journals Bioremediation of Petroleum Hydrocarbons Contaminated Soil using Bio piles System

2019 ◽  
Vol 16 (1) ◽  
pp. 0185 ◽  
Author(s):  
Jabbar Et al.
Keyword(s):  
Surface Tension ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Bacterial Culture ◽  
Pilot Scale ◽  
Ex Situ ◽  
Total Petroleum Hydrocarbons ◽  
Bacterial Strains ◽  
Mixed Bacterial Culture ◽  
Surface Tension Reduction

This study was focused on biotreatment of soil which polluted by petroleum compounds (Diesel) which caused serious environmental problems. One of the most effective and promising ways to treat diesel-contaminated soil is bioremediation. It is a choice that offers the potential to destroy harmful pollutants using biological activity. Four bacterial strains were isolated from diesel contaminated soil samples. The isolates were identified by the Vitek 2 system, as Sphingomonas paucimobilis, Pentoae species, Staphylococcus aureus, and Enterobacter cloacae. The potential of biological surfactant production was tested using the Sigma 703D stand-alone tensiometer showed that these isolates are biological surfactant producers. The better results of the surface tension reduction test were obtained using the mixed bacterial culture which reduced the surface tension of the medium from 66mN/m to 33.89mN/m. For further evidence of the biodegradation effect of these isolates individually and as a mixed culture, which was supported by the use of Gas-Chromatography technology confirming the occurrence of biodegradation. The capability of mixed bacterial culture was examined to remediate the diesel contaminated soil in bio piles system. Two pilot scale bio piles (25 kg soil each) were constructed containing soils contaminated with approximately 2140 mg/kg total petroleum hydrocarbons (TPHs). Both systems were equipped with oxygen to provide aerobic conditions, incubated at ambient temperature and weekly sampling within 35 days (during summer season). Overall 75.71 % of the total petroleum hydrocarbons were removed from the amended soil and 33.18 % of the control soil at the end of study period. The study concluded that the ex-situ bioremediation (bio piles) is a good option for treating the soil contaminated with diesel as economical and environmentally friendly.

scholarly journals Biosurfactant-Producing Lactobacilli: Screening, Production Profiles, and Effect of Medium Composition

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Eduardo J. Gudiña ◽  
José A. Teixeira ◽  
Lígia R. Rodrigues
Keyword(s):  
Surface Tension ◽  
Optimization Procedure ◽  
Nitrogen Sources ◽  
Culture Broth ◽  
Medium Composition ◽  
Biosurfactant Production ◽  
Standard Medium ◽  
Meat Extract ◽  
Surface Tension Reduction ◽  
Dairy Plant

Biosurfactant production was screened in four lactobacilli strains. The highest biosurfactant production (excreted and cell-bound biosurfactants) was achieved withLactobacillus paracaseissp.paracaseiA20, a strain isolated from a Portuguese dairy plant, with a decrease in the surface tension of 6.4 mN m−1and 22.0 mN m−1, respectively. Biosurfactant production by this strain was evaluated under different culture broth compositions. The use of different nitrogen sources revealed that yeast extract is essential for bacterial growth, while peptone is crucial for biosurfactant synthesis. For biosurfactant production, the use of peptone and meat extract yielded a higher production when compared to the standard medium, with a surface tension reduction of 24.5 mN m−1Furthermore, experiments were also conducted in a reactor with pH and temperature control. Biomass and biosurfactant production in bioreactor was higher comparing with the experiments conducted in shake flaks. The optimization procedure adopted in the current work was found to improve the biosurfactant production and opened new perspectives for the use ofL. paracaseissp.paracaseiA20 as a promising biosurfactant-producer.

scholarly journals Pseudomonas fluorescens: A Bioaugmentation Strategy for Oil-Contaminated and Nutrient-Poor Soil

2020 ◽  
Vol 17 (19) ◽  
pp. 6959
Author(s):  
Eduardo Jahir Gutiérrez ◽  
María del Rosario Abraham ◽  
Juan Carlos Baltazar ◽  
Guadalupe Vázquez ◽  
Eladio Delgadillo ◽  
...  
Keyword(s):  
Surface Tension ◽  
Pseudomonas Fluorescens ◽  
Yeast Extract ◽  
Petroleum Hydrocarbons ◽  
Respiratory Activity ◽  
Culture Conditions ◽  
Total Petroleum Hydrocarbons ◽  
Solid Culture ◽  
Soil Extracts ◽  
Poor Soil

Bioremediation technology is one of the most profitable and sustainable strategies for remediating soils contaminated with hydrocarbons. This study focuses on assessing the influence of biostimulation and bioaugmentation with Pseudomonas fluorescens to contribute to the removal of total petroleum hydrocarbons (TPHs) of a soil. Laboratory studies were carried out (measurements of emitted CO2, surface tension, and residual TPH) to select the best bioaugmentation and biostimulation treatment. The sources of C, N, and P were glucose–yeast extract, NH4Cl–NaNO3, and K2HPO4–K3PO4, respectively. The effect of culture conditions on the reduction of TPH and respiratory activity was evaluated through a factorial design, 23, in a solid culture system. After 80 days of incubation, it was observed that treatments of yeast extract–NH4Cl–K2HPO4 (Y4) and glucose–NaNO3–K3PO4 (Y5) presented a higher level of TPH removal (20.91% and 20.00% degradation of TPH, respectively). Biostimulation favors the production of biosurfactants, indirectly measured by the change in surface tension in the soil extracts. The treatments Y4 and Y5 showed a lower change value of the surface tension (23.15 and 23.30 mN·m−1 at 25 °C). A positive correlation was determined between the change in surface tension and the removal of TPH; hence there was a contribution of the biosurfactants produced to the removal of hydrocarbons.

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