Bioremediation of Petroleum Hydrocarbons Contaminated Soil using  Bio piles System

This study was focused on biotreatment of soil which polluted by petroleum compounds (Diesel) which caused serious environmental problems. One of the most effective and promising ways to treat diesel-contaminated soil is bioremediation. It is a choice that offers the potential to destroy harmful pollutants using biological activity. Four bacterial strains were isolated from diesel contaminated soil samples. The isolates were identified by the Vitek 2 system, as Sphingomonas paucimobilis, Pentoae species, Staphylococcus aureus, and Enterobacter cloacae. The potential of biological surfactant production was tested using the Sigma 703D stand-alone tensiometer showed that these isolates are biological surfactant producers. The better results of the surface tension reduction test were obtained using the mixed bacterial culture which reduced the surface tension of the medium from 66mN/m to 33.89mN/m. For further evidence of the biodegradation effect of these isolates individually and as a mixed culture, which was supported by the use of Gas-Chromatography technology confirming the occurrence of biodegradation. The capability of mixed bacterial culture was examined to remediate the diesel contaminated soil in bio piles system. Two pilot scale bio piles (25 kg soil each) were constructed containing soils contaminated with approximately 2140 mg/kg total petroleum hydrocarbons (TPHs). Both systems were equipped with oxygen to provide aerobic conditions, incubated at ambient temperature and weekly sampling within 35 days (during summer season). Overall 75.71 % of the total petroleum hydrocarbons were removed from the amended soil and 33.18 % of the control soil at the end of study period. The study concluded that the ex-situ bioremediation (bio piles) is a good option for treating the soil contaminated with diesel as economical and environmentally friendly.

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Bioremediation of Petroleum Hydrocarbons Contaminated Soil using Bio piles System

Baghdad Science Journal ◽

10.21123/bsj.2019.16.1(suppl.).0185 ◽

2019 ◽

Vol 16 (1(Suppl.)) ◽

pp. 0185

Author(s):

Jabbar Et al.

Keyword(s):

Surface Tension ◽

Contaminated Soil ◽

Petroleum Hydrocarbons ◽

Bacterial Culture ◽

Pilot Scale ◽

Ex Situ ◽

Total Petroleum Hydrocarbons ◽

Bacterial Strains ◽

Mixed Bacterial Culture ◽

Surface Tension Reduction

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Bioremediation of Soil Contaminated with Diesel using Biopile system

Al-Khwarizmi Engineering Journal ◽

10.22153/https://doi.org/10.22153/kej.2018.12.009 ◽

2018 ◽

Vol 14 (3) ◽

pp. 48-56

Author(s):

Noor Mohsen Jabbar ◽

Estabriq Hasan Kadhim ◽

Alaa Kareem Mohammed

Keyword(s):

Contaminated Soil ◽

Contaminated Soils ◽

Petroleum Hydrocarbons ◽

Bacterial Culture ◽

Atmospheric Temperature ◽

Pilot Scale ◽

Ex Situ ◽

Total Petroleum Hydrocarbons ◽

Bacterial Inoculum ◽

Amended Soil

This study was focused on biotreatment of soil which polluted by petroleum compounds (Diesel) which caused serious environmental problems. One of the most effective and promising ways to treat diesel-contaminated soil is bioremediation. It is a choice that offers the potential to destroy harmful pollutants using biological activity. The capability of mixed bacterial culture was examined to remediate the diesel-contaminated soil in bio piling system. For fast ex-situ treatment of diesel-contaminated soils, the bio pile system was selected. Two pilot scale bio piles (25 kg soil each) were constructed containing soils contaminated with approximately 2140 mg/kg total petroleum hydrocarbons (TPHs). The amended soil: (contaminated soil with the addition of nutrients and bacterial inoculum), where the soil was mixed with 1.5% of sawdust, then supplied with the necessary nutrients and watered daily to provide conditions promoting microorganism growth. Unamended soil was prepared as a control (contaminated soil without addition). Both systems were equipped with oxygen to provide aerobic conditions, incubated at atmospheric temperature and weekly sampling within 35 days. Overall 75% of the total petroleum hydrocarbons were removed from the amended soil and 38 % of the control soil at the end of study period. The study concluded that ex-situ experiment (Bio pile) is a preferable, economical, and environmentally friendly procedure, thus representing a good option for the treatment of soil contaminated with diesel.

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Bioremediation of Soil Contaminated with Diesel using Biopile system

Al-Khwarizmi Engineering Journal ◽

10.22153/kej.2018.12.009 ◽

2018 ◽

Vol 14 (3) ◽

pp. 48-56 ◽

Cited By ~ 2

Author(s):

Noor Mohsen Jabbar ◽

Estabriq Hasan Kadhim ◽

Alaa Kareem Mohammed

Keyword(s):

Contaminated Soil ◽

Contaminated Soils ◽

Petroleum Hydrocarbons ◽

Bacterial Culture ◽

Atmospheric Temperature ◽

Pilot Scale ◽

Ex Situ ◽

Total Petroleum Hydrocarbons ◽

Bacterial Inoculum ◽

Amended Soil

This study was focused on biotreatment of soil which polluted by petroleum compounds (Diesel) which caused serious environmental problems. One of the most effective and promising ways to treat diesel-contaminated soil is bioremediation. It is a choice that offers the potential to destroy harmful pollutants using biological activity. The capability of mixed bacterial culture was examined to remediate the diesel-contaminated soil in bio piling system. For fast ex-situ treatment of diesel-contaminated soils, the bio pile system was selected. Two pilot scale bio piles (25 kg soil each) were constructed containing soils contaminated with approximately 2140 mg/kg total petroleum hydrocarbons (TPHs). The amended soil: (contaminated soil with the addition of nutrients and bacterial inoculum), where the soil was mixed with 1.5% of sawdust, then supplied with the necessary nutrients and watered daily to provide conditions promoting microorganism growth. Unamended soil was prepared as a control (contaminated soil without addition). Both systems were equipped with oxygen to provide aerobic conditions, incubated at atmospheric temperature and weekly sampling within 35 days. Overall 75% of the total petroleum hydrocarbons were removed from the amended soil and 38 % of the control soil at the end of study period. The study concluded that ex-situ experiment (Bio pile) is a preferable, economical, and environmentally friendly procedure, thus representing a good option for the treatment of soil contaminated with diesel.

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Biodegradation of Total Petroleum Hydrocarbons in Soil: Isolation and Characterization of Bacterial Strains from Oil Contaminated Soil

Applied Sciences ◽

10.3390/app10124173 ◽

2020 ◽

Vol 10 (12) ◽

pp. 4173 ◽

Cited By ~ 1

Author(s):

Runkai Wang ◽

Baichun Wu ◽

Jin Zheng ◽

Hongkun Chen ◽

Pinhua Rao ◽

...

Keyword(s):

Contaminated Soil ◽

Petroleum Hydrocarbons ◽

Ph Value ◽

Optimal Growth ◽

Growth Conditions ◽

Total Petroleum Hydrocarbons ◽

Impact Factors ◽

Growth Media ◽

Bacterial Strains ◽

Isolation And Characterization

In this study, we isolated seven strains (termed BY1–7) from polluted soil at an oil station and evaluated their abilities to degrade total petroleum hydrocarbons (TPHs). Following 16 rRNA sequence analysis, the strains were identified as belonging to the genera Bacillus, Acinetobacter, Sphingobium, Rhodococcus, and Pseudomonas, respectively. Growth characterization studies indicated that the optimal growth conditions for the majority of the strains was at 30 °C, with a pH value of approximately 7. Under these conditions, the strains showed a high TPH removal efficiency (50%) after incubation in beef extract peptone medium for seven days. Additionally, we investigated the effect of different growth media on growth impact factors that could potentially affect the strains’ biodegradation rates. Our results suggest a potential application for these strains to facilitate the biodegradation of TPH-contaminated soil.

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Biodegradation of endosulfan-contaminated soil in a pilot-scale reactor-bioaugmented with mixed bacterial culture

Journal of Environmental Science and Health Part B ◽

10.1080/03601230701465940 ◽

2007 ◽

Vol 42 (6) ◽

pp. 707-715 ◽

Cited By ~ 7

Author(s):

Mathava Kumar ◽

Ligy Philip

Keyword(s):

Contaminated Soil ◽

Bacterial Culture ◽

Pilot Scale ◽

Mixed Bacterial Culture ◽

Pilot Scale Reactor ◽

Scale Reactor

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Assessment of Biodegradation Efficiency of Polychlorinated Biphenyls (PCBs) and Petroleum Hydrocarbons (TPH) in Soil Using Three Individual Bacterial Strains and Their Mixed Culture

Molecules ◽

10.3390/molecules25030709 ◽

2020 ◽

Vol 25 (3) ◽

pp. 709 ◽

Cited By ~ 5

Author(s):

Teresa Steliga ◽

Katarzyna Wojtowicz ◽

Piotr Kapusta ◽

Joanna Brzeszcz

Keyword(s):

Polychlorinated Biphenyls ◽

Mixed Culture ◽

Petroleum Hydrocarbons ◽

Rhodococcus Erythropolis ◽

Ex Situ ◽

Total Petroleum Hydrocarbons ◽

Soil Toxicity ◽

Bacterial Strains ◽

Sterile Soil ◽

Rhodococcus Sp

Biodegradation is one of the most effective and profitable methods for the elimination of toxic polychlorinated biphenyls (PCBs) and total petroleum hydrocarbons (TPH) from the environment. In this study, aerobic degradation of the mentioned pollutants by bacterial strains Mycolicibacterium frederiksbergense IN53, Rhodococcus erythropolis IN129, and Rhodococcus sp. IN306 and mixed culture M1 developed based on those strains at 1:1:1 ratio was analyzed. The effectiveness of individual strains and of the mixed culture was assessed based on carried out respirometric tests and chromatographic analyses. The Rhodococcus sp. IN306 turned out most effective in terms of 18 PCB congeners biodegradation (54.4%). The biodegradation index was decreasing with an increasing number of chlorine atoms in a molecule. Instead, the Mycolicobacterium frederiksbergense IN53 was the best TPH degrader (37.2%). In a sterile soil, contaminated with PCBs and TPH, the highest biodegradation effectiveness was obtained using inoculation with mixed culture M1, which allowed to reduce both the PCBs (51.8%) and TPH (34.6%) content. The PCBs and TPH biodegradation capacity of the defined mixed culture M1 was verified ex-situ with prism method in a non-sterile soil polluted with aged petroleum hydrocarbons (TPH) and spent transformer oil (PCBs). After inoculation with mixed culture M1, the PCBs were reduced during 6 months by 84.5% and TPH by 70.8% as well as soil toxicity was decreased.

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Effect of compost in phytoremediation of diesel-contaminated soils

Water Science & Technology ◽

10.2166/wst.2001.0102 ◽

2001 ◽

Vol 43 (2) ◽

pp. 291-295 ◽

Cited By ~ 26

Author(s):

J. Vouillamoz ◽

M. W. Milke

Keyword(s):

Moisture Content ◽

Contaminated Soil ◽

Contaminated Soils ◽

Petroleum Hydrocarbons ◽

Dilution Effect ◽

Screening Tests ◽

Total Petroleum Hydrocarbons ◽

Controlled Environment ◽

Grass Growth ◽

The Impact

The effect of compost on phytoremediation of diesel-contaminated soils was investigated using 130 small (200 g) containers in two screening tests. The experiments were conducted in a controlled environment using ryegrass from seed. Containers were destructively sampled at various times and analyzed for plant mass and total petroleum hydrocarbons. The results indicate that the presence of diesel reduces grass growth, and that compost helps reduced the impact of diesel on grass growth. The addition of compost helps increase diesel loss from the soils both with and without grass, though the addition of grass leads to lower diesel levels compared with controls. A second set of experiments indicates that the compost helps in phytoremediation of diesel-contaminated soil independent of the dilution effect that compost addition has. The results indicate that the compost addition allowed diesel loss down to 200 mg TPH/kg even though the compost would be expected to hold the diesel more tightly in the soil/compost mixture. The simplicity of the screening tests led to difficulties in controlling moisture content and germination rates. The conclusion of the research is that the tilling of compost into soils combined with grass seeding appears to be a valuable option for treating petroleum-contaminated soils.

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Evolution of humic acids during ex situ bioremediation on a pilot level: The added value of the microbial activity

Journal of the Serbian Chemical Society ◽

10.2298/jsc190916131z ◽

2020 ◽

Vol 85 (6) ◽

pp. 821-830

Author(s):

Aleksandra Zeradjanin ◽

Jelena Avdalovic ◽

Marija Ljesevic ◽

Olivera Tesic ◽

Srdjan Miletic ◽

...

Keyword(s):

Humic Acids ◽

Petroleum Hydrocarbons ◽

Added Value ◽

Ex Situ ◽

Total Petroleum Hydrocarbons ◽

Number Of Microorganisms ◽

The Fourier Transform ◽

The Relationship ◽

Acid Structure ◽

Qualitative Changes

Environmental pollution is a global problem, while bioremediation technology removes pollutants from the environment using microorganisms. This study was aimed at investigating how a bioremediation process affected soil humification. In soil polluted with petroleum and its derivatives that was submitted to bioremediation, besides the total petroleum hydrocarbons and the number of microorganisms, quantitative and qualitative changes of isolated humic acids were determined during the process. The bioremediation of 150 m3 of polluted soil lasted 150 days. The level of total petroleum hydrocarbons decreased by 86.6 %, while the level of humic acids increased by 26.5 %. The elemental analysis showed the reduction of C and the H/C ratio and the increase of O and the O/C ratio of isolated humic acids during the process. The ratio of absorbencies at 465 and 665 nm also increased. Based on this and the Fourier-transform infrared spectra, it was shown that the humic acids isolated at the end of bioremediation were enriched with oxygen functional groups and aromatic structures. This study provides one of the first insights into the relationship between bioremediation and humification, as well as evidence of how hydrocarbon-degrading microorganisms have a significant influence on changes to humic acid structure during bioremediation.

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Soil Microcosms for Bioaugmentation With Fungal Isolates to Remediate Petroleum Hydrocarbon-contaminated Soil

10.21203/rs.3.rs-1086969/v1 ◽

2021 ◽

Author(s):

Dalel Daâssi ◽

Fatimah Qabil Almaghribi

Keyword(s):

Contaminated Soil ◽

Petroleum Hydrocarbons ◽

Fungal Growth ◽

Total Petroleum Hydrocarbons ◽

Fungal Culture ◽

Soil Microcosms ◽

Fungal Isolates ◽

Petroleum Contaminated Soil ◽

Decaying Wood ◽

Native Strains

Abstract The aim of this work was to isolate indigenous PAH degrading-fungi from petroleum contaminated soil and exogenous ligninolytic strains from decaying-wood, with the ability to secrete diverse enzyme activity. A total of ten ligninolytic fungal isolates and two native strains, has been successfully isolated, screened and identified. The phylogenetic analysis revealed that the indigenous fungi (KBR1 and KB8) belong to the genus Aspergillus niger and tubingensis. While the ligninolytic exogenous PAH-degrading strains namely KBR1-1, KB4, KB2 and LB3 were affiliated to different genera like Syncephalastrum sp, Paecilomyces formosus, Fusarium chlamydosporum, and Coniochaeta sp., respectively. Basis on the taxonomic analysis, enzymatic activities and the hydrocarbons removal rates, single fungal culture employing the strain LB3, KB4, KBR1 and the mixed culture (LB3+KB4) were selected to be used in soil microcosms treatments. The Total petroleum hydrocarbons (TPH), fungal growth rates, BOD5/COD ratios and GC-MS analysis, were determined in all soil microcosmos treatments (SMT) and compared with those of the control (SMU). After 60 days of culture incubation, the highest rate of TPH degradation was recorded in SMT[KB4] by approximately 92±2.35% followed by SMT[KBR1] then SMT[LB3+KB4] with 86.66±1.83% and 85.14±2.21%, respectively.

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