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2022 ◽  
Vol 9 ◽  
Author(s):  
Minghua Tang ◽  
Kinzie L. Matz ◽  
Lillian M. Berman ◽  
Kathryn N. Davis ◽  
Edward L. Melanson ◽  
...  

Background: An urgent need exists for evidence-based dietary guidance early in life, particularly regarding protein intake. However, a significant knowledge gap exists in the effects of protein-rich foods on growth and development during early complementary feeding.Methods: This is a randomized controlled trial of infant growth and gut health (primary outcomes). We directly compare the effects of dietary patterns with common protein-rich foods (meat, dairy, plant) on infant growth trajectories and gut microbiota development (monthly assessments) during early complementary feeding in both breast- and formula-fed infants. Five-month-old infants (up to n = 300) are randomized to a meat-, dairy-, plant-based complementary diet or a reference group (standard of care) from 5 to 12 months of age, with a 24-month follow-up assessment. Infants are matched for sex, mode of delivery and mode of feeding using stratified randomization. Growth assessments include length, weight, head circumference and body composition. Gut microbiota assessments include both 16S rRNA profiling and metagenomics sequencing. The primary analyses will evaluate the longitudinal effects of the different diets on both anthropometric measures and gut microbiota. The secondary analysis will evaluate the potential associations between gut microbiota and infant growth.Discussion: Findings are expected to have significant scientific and health implications for identifying beneficial gut microbial changes and dietary patterns and for informing dietary interventions to prevent the risk of overweight and later obesity, and promote optimal health.Clinical Trial Registration:www.ClinicalTrials.gov, identifier: NCT05012930.


Nutrients ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 162
Author(s):  
Winston J. Craig ◽  
Cecilia J. Brothers ◽  
Reed Mangels

A growing number of people are seeking a non-dairy plant-based beverage both for their personal health, and for the health of the planet. The aim of this study was to conduct a cross-sectional survey of single-serve plant-based beverages to assess their nutritional content and health profile. A total of 51 non-dairy plant-based beverages were analyzed from the nutrition label listed on the commercial package. The various beverages contained extracts of soy (n = 14), almonds (n = 13), oats (n = 12), peas (n = 7), banana (n = 2), coconut (n = 2), and rice (n = 1). Almost one-half (45%) of the single-serve beverages had 5 g or more of protein/serving. A total of 75% and 65% of the single-serve beverages had calcium and vitamin B12 levels, respectively, fortified to at least 20% of the Daily Value (DV), while only 28% had vitamin D fortification at the 20% DV level. Two-thirds of the single-serve beverages had high sugar levels, while 39% were low in sodium, 63% were low in fat, and 96% were low in saturated fat. The single-serve plant-based beverages had more protein, calcium, vitamin B12, and sugar but less fat than the non-dairy, multi-serve plant-based beverages/ serving. A limited number of single-serve beverages met the requirements of school meal programs.


Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 274
Author(s):  
Siniša Matutinović ◽  
Ante Rako ◽  
Milna Tudor Kalit ◽  
Samir Kalit

Lećevački cheese is a traditional Croatian hard cheese, which belongs to the group of hard Mediterranean cheeses produced from mixed milk (cow and sheep). The aim of this research was to determine the influence of different ratios and compositions of sheep milk on the composition and yield of Lećevački cheese. A total of 15 batches of Lećevački cheese were selected containing different ratios of sheep and cow milk from the regular production of a dairy plant. The ratio of sheep milk was as follows: up to 39%, from 40 to 44%, and from 45 to 50%. For each ratio, five batches were randomly selected. A higher ratio of sheep milk caused a noticeable increase in fat, protein, lactose, and total solids content, while the content of solids-not-fat significantly (p < 0.05) increased. A similar trend was found for casein content (p < 0.1). The highest ratio of sheep milk in mixed milk increased (p < 0.05) the protein content by almost 1%. However, the results showed that it is not reasonable to increase the sheep milk ratio in mixed milk above 44% (v/v) because it causes a higher (p < 0.01) moisture content in the cheese, as well as a lower fat content (p < 0.01) and fat recovery (p = 0.07) during the manufacturing of Lećevački cheese.


Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2876
Author(s):  
Angela Maria Catania ◽  
Tiziana Civera ◽  
Pierluigi Aldo Di Ciccio ◽  
Maria Ausilia Grassi ◽  
Patrizia Morra ◽  
...  

Processed cheese is a commercial product characterized by high microbiological stability and extended shelf life obtained through the application of severe heat treatment. However, spore-forming bacteria can survive through thermal processes. Among them, microorganisms belonging to Bacillus genus have been reported. In this study, we examined the microbiological population of the first hours’ production of processed cheeses in an Italian dairy plant during two seasons, between June and October 2020. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to identify bacteria colonies, allowing the isolation of Bacillus cereus and Bacillussubtilis strains. These results were further confirmed by amplification and sequencing of 16 rRNA bacterial region. A multi-locus sequence type (MLST) analysis was performed to assess the genetic similarity among a selection of isolates. The fourteen B. cereus strains showed two sequence types: ST-32 was observed in only one strain and the ST-371 in the remaining thirteen isolates. On the contrary, all twenty-one B. subtlis strains, included in the study, showed a new allelic profile for the pycA gene, resulting in a new sequence type: ST-249. For B. cereus strains, analysis of toxin genes was performed. All isolates were positive for nheABC, entFM, and cytK, while hblABCD, bceT, and ces were not detected. Moreover, the biofilm-forming ability of B. cereus and B. subtilis strains was assessed, and all selected isolates proved to be biofilm formers (most of them were stronger producers). Considering the genetical similarity between isolates, jointly with the capacity to produce biofilm, the presence of a recurring Bacillus population could be hypothesized.


Nutrients ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 4069
Author(s):  
Winston J. Craig ◽  
Cecilia J. Brothers

Yogurt is considered a healthy, nutritious food in many cultures. With a significant number of people experiencing dairy intolerance, and support for a more sustainable diet, consumer demand for dairy alternatives has surged. The aim of this study was to conduct a cross-sectional survey of plant-based yogurt alternatives to assess their nutritional content and health profile. A total of 249 non-dairy yogurt alternatives were analyzed from the nutrition label listed on the commercial package. The various yogurt alternatives contained extracts of coconut (n = 79), almonds (n = 62), other nuts or seeds (n = 20), oats (n = 20), legumes (n = 16), and mixed blends (n = 52). At least one-third of the yogurt alternatives had 5 g or more of protein/serving. Only 45% of the yogurt alternatives had calcium levels fortified to at least 10% of daily value (DV), while only about one in five had adequate vitamin D and B12 fortification at the 10% DV level. One-half of the yogurt alternatives had high sugar levels, while 93% were low in sodium. Except for the coconut-based products, the yogurts were not high in fat or saturated fat. The yogurt alternatives were not fortified as frequently or to the same levels as the corresponding non-dairy, plant-based beverages.


Foods ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1859
Author(s):  
Federica Biolcati ◽  
Ilario Ferrocino ◽  
Maria Teresa Bottero ◽  
Alessandra Dalmasso

Robiola di Roccaverano is a Protected Designation of Origin (PDO) cheese from the Piedmont region of Italy. In this study, the mycobiota occurring during Robiola di Roccaverano production was elucidated. Samples of milk, Natural Milk Cultures (NMC), curd, 5- and 15-days ripened cheese were collected from one dairy plant and the mycobiota was analyzed by the metataxonomic approach. Milk samples showed a high diversity and Cladosporium, Kluyveromyces marxianus, Geotrichum candidum and Debaryomyces hansenii were found with higher relative abundance. This mycobiota remains quite stable in NMC and curd matrices although the relative abundance of K. marxianus and G. candidum yeasts increased significantly and shaped the fungal composition of 5- and 15-day ripened cheese.


2021 ◽  
Vol 14 ◽  
pp. 89-99
Author(s):  
Y.V. Matveichuk ◽  
◽  
D.V. Stanishevskii ◽  

Aim. Development of a complex of detergents (acidic, alkaline, enzymatic, chlorine-containing, surfactant-containing additives) for membrane units used in the dairy industry. Materials and Methods. Methods for controlling the concentration of active substances in concentrates of agents are given on the example of KATELON 202 - determination of total acidity by the method of acid-base potentiometric titration (in terms of HNO3) and KATELON 114 – determination of the mass fraction of active chlorine by redox titration. Results. A complex of detergents for membrane plants (ultrafiltration (UV, UF), microfiltration (MF, MF), nanofiltration (NF, NF), reverse osmosis (RO, RO)), used in the dairy industry and including alkaline non-foaming, medium-, low-foam products KATELON 109, KATELON 110, KATELON 115; alkaline chlorine-containing non-foamy agent KATELON 114; acid non-foamy KATELON 202, KATELON 207, KATELON 208; high-foam neutral additives KATELON 308 and KATELON 309 – enhancers of the washing effect for KATELON 109, KATELON 110, KATELON 115; preservative non-foaming additive KATELON 310 for protection from microbiological contamination; enzyme medium supplement KATELON 601. The composition of all products, their brief characteristics and physicochemical indicators, as well as an approximate program of washing with a complex of developed preparations are given. The dependences of pH and electrical conductivity were obtained for KATELON 109, KATELON 110, KATELON 115, KATELON 202, KATELON 207, KATELON 208. Conclusion. The complex of detergents is successfully used at Kopylsky Butter Cheese Plant, Lyubansky Cheese Making Plant, Minsk Dairy Plant No. 1, Verkhnedvinsky Butter and Cheese Plant, Molodechno Dairy Plant, Volozhin production site of Minsk Dairy Plant No. 1, Tolochin Branch of Lepel Dairy Canning Plant.


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