scholarly journals Analisis Bioinformatika dan Ekspresi Protein Rekombinan Hemagglutinin Domain Globular dari Virus H5N1 Indonesia pada Eschericia coli BL21 (DE3) sebagai Komponen Vaksin Subunit Influenza

al-Kimiya ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 59-67
Author(s):  
Muhammad Yusuf ◽  
Rima Handiyani ◽  
Shinta Kusumawardani ◽  
Idar Idar ◽  
Umi Baroroh ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Avian Influenza ◽  
Escherichia Coli Bl21 ◽  
Pathogenic Avian Influenza ◽  
Virus H5n1 ◽  
Sds Page ◽  
High Pathogenic ◽  
High Pathogenic Avian Influenza

 Flu burung merupakan salah satu penyakit zoonosis yang patut diwaspadai di Indonesia, khususnya galur High Pathogenic Avian Influenza (HPAI) karena dapat mematikan jika menular kepada manusia. Penggunaan vaksin influenza pada unggas, merupakan langkah preventif terhadap evolusi virus yang berbahaya dan juga penyebarannya. Selama ini, Indonesia masih menggunakan seed vaksin impor yang berasal dari luar Indonesia. Namun, karena Indonesia merupakan negara yang berada di garis khatulistiwa, karakteristik virus bisa berbeda dengan virus dari nothern-hemispere maupun southern-hemispere. Mengingat hal tersebut, Indonesia harus mengembangkan vaksin influenza menggunakan galur virus lokal. Berbeda dengan vaksin whole virus, vaksin rekombinan memiliki keunggulan dari sisi kemurnian, kecepatan produksi, dan kesesuaian galur terhadap virus yang beredar saat diperlukan. Penelitian ini bertujuan untuk menganalisis sekuen hemagglutinin (HA) Indonesia dengan strain lainnya serta mengekspresikkan protein HA1 rekombinan pada Escherichia coli  BL21 (DE3). Galur yang digunakan pada studi ini berasal dari virus H5N1 (A/Indonesia/05/05), khususnya bagian domain globular dari HA1. Sekuen HA1 bervariasi antara strain Indonesia dengan nothern-hemispere maupun southern-hemispere dan merupakan protein yang terpapar ke luar virus. Gen HA1 disisipkan pada vektor pET-28a, kemudian plasmid diisolasi menggunakan meoe manniatis, setelah itu diekspresikan dengan induksi 1 mM IPTG selama 4 jam. Protein HA1 telah berhasil diekspresikan secara intraseluler dan telah dikonfirmasi pada berat molekul 40 kDa menggunakan SDS-PAGE. Penelitian ini dapat digunakan untuk mengembangkan vaksin subunit yang lebih spesifik terhadap virus yang beredar di lapangan.

scholarly journals Dynamics of low and high pathogenic avian influenza in wild and domestic bird populations

2015 ◽  
Vol 10 (1) ◽  
pp. 104-139 ◽  
Author(s):  
Necibe Tuncer ◽  
Juan Torres ◽  
Maia Martcheva ◽  
Michael Barfield ◽  
Robert D. Holt
Keyword(s):  
Avian Influenza ◽  
Bird Populations ◽  
Pathogenic Avian Influenza ◽  
Domestic Bird ◽  
High Pathogenic ◽  
High Pathogenic Avian Influenza

Modelling high pathogenic avian influenza outbreaks in the commercial poultry industry

2019 ◽  
Vol 126 ◽  
pp. 59-71 ◽  
Author(s):  
Belinda Barnes ◽  
Angela Scott ◽  
Marta Hernandez-Jover ◽  
Jenny-Ann Toribio ◽  
Barbara Moloney ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Poultry Industry ◽  
Pathogenic Avian Influenza ◽  
Commercial Poultry ◽  
Influenza Outbreaks ◽  
High Pathogenic ◽  
High Pathogenic Avian Influenza

scholarly journals Strategies to control high pathogenic avian influenza (HPAI) in the Belgian poultry sector

2009 ◽  
Vol 25 (5-6-1) ◽  
pp. 373-385 ◽  
Author(s):  
Y. Vandendriessche ◽  
X. Gellynck ◽  
H. Saatkamp ◽  
J. Viaene
Keyword(s):  
Avian Influenza ◽  
Control Strategies ◽  
Control Measures ◽  
Economic Losses ◽  
Economic Damage ◽  
Correct Identification ◽  
Economic Point ◽  
Pathogenic Avian Influenza ◽  
High Pathogenic ◽  
High Pathogenic Avian Influenza

High Pathogenic Avian Influenza (HPAI) may pose a major threat for the Belgium poultry sector, as an outbreak of HPAI results in tremendous economic losses. In order to reduce the economic damage for an outbreak, different strategies to control HPAI are evaluated. In a first stage the structure of the Belgium poultry sector is described and risks are analyzed. The actual risks are dependent of the intensive character of poultry farming in Belgium, the large number of transport movements of living poultry, the presence of sensitive nature areas and the border with the Netherlands where the poultry density is even larger. In a second stage the possible intervention strategies are evaluated. Starting from the current regulation, two strategies are worked out: stamping out and emergency vaccination. The success of emergency vaccination is associated with the correct identification of compartments at risk, prompt deployment of emergency vaccines, rapid enforcement of appropriate complementary control measures and also the level of being ready. In a third stage an economic analysis of control strategies for HPAI outbreaks is made. Results suggest that from an economic point of view, stamping-out is at farm level a better option then emergency vaccination within the current context.

scholarly journals Ammonia Disinfection of Hatchery Waste for Elimination of Single-Stranded RNA Viruses

2011 ◽  
Vol 77 (12) ◽  
pp. 3960-3966 ◽  
Author(s):  
Eva Emmoth ◽  
Jakob Ottoson ◽  
Ann Albihn ◽  
Sándor Belák ◽  
Björn Vinnerås
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Rna Viruses ◽  
Hepatitis E ◽  
Inactivation Kinetics ◽  
Pathogenic Avian Influenza ◽  
Pathogenic Avian Influenza Virus ◽  
High Pathogenic ◽  
High Pathogenic Avian Influenza

ABSTRACTHatchery waste, an animal by-product of the poultry industry, needs sanitation treatment before further use as fertilizer or as a substrate in biogas or composting plants, owing to the potential presence of opportunistic pathogens, including zoonotic viruses. Effective sanitation is also important in viral epizootic outbreaks and as a routine, ensuring high hygiene standards on farms. This study examined the use of ammonia at different concentrations and temperatures to disinfect hatchery waste. Inactivation kinetics of high-pathogenic avian influenza virus H7N1 and low-pathogenic avian influenza virus H5N3, as representatives of notifiable avian viral diseases, were determined in spiked hatchery waste. Bovine parainfluenza virus type 3, feline coronavirus, and feline calicivirus were used as models for other important avian pathogens, such as Newcastle disease virus, infectious bronchitis virus, and avian hepatitis E virus. Bacteriophage MS2 was also monitored as a stable indicator. Coronavirus was the most sensitive virus, with decimal reduction (D) values of 1.2 and 0.63 h after addition of 0.5% (wt/wt) ammonia at 14 and 25°C, respectively. Under similar conditions, high-pathogenic avian influenza H7N1 was the most resistant, withDvalues of 3.0 and 1.4 h. MS2 was more resistant than the viruses to all treatments and proved to be a suitable indicator of viral inactivation. The results indicate that ammonia treatment of hatchery waste is efficient in inactivating enveloped and naked single-stranded RNA viruses. Based on theDvalues and confidence intervals obtained, guidelines for treatment were proposed, and one was successfully validated at full scale at a hatchery, with MS2 added to hatchery waste.

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