scholarly journals Linoleic acid isomerization ability of Lactobacillus spp. isolated from Vietnamese human intestinal origins

2020 ◽  
Vol 18 (3) ◽  
pp. 445-453
Author(s):  
Tran Xuan Thach ◽  
Ha Thi Thu ◽  
Vu Thi Hien ◽  
Hoang The Hung ◽  
Nguyen Thi Hoa ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Double Bonds ◽  
Liquid Form ◽  
Isomerase Activity ◽  
Acid Isomerization ◽  
Hydroxy Groups ◽  
Substrate Concentrations ◽  
Lactobacillus Spp ◽  
Mrs Medium ◽  
Linoleate Isomerase

Conjugated linoleic acid (CLA) have been shown to exert numerous health benefits, including anti-carcinogenic, anti-atherogenic, anti-diabetic, antiobesity, cholesterol reducing, antioxidant, anti-microbial, immune system modulator and growth-stimulating properties. In human, CLA is produced from Linoleic acid (LA) by gut bacteria. In this study, nineteen Lactobacillus (Lac.) strains isolated from human feces were studied to determine their ability to metabolize LA. The bacteria were grown in the liquid form of anaerobic MRS medium supplemented with 0.5 mg/mL LA. The linoleate isomerase activity in bacteria grown on MRS medium was determined by Gas chromatograpy. The results indicated that 4 out of 19 strains, including strains Lac.02, Lac.05, Lac.14 and Lac.16 are capable of producing about 40-50 μg/mL CLA from LA. Among them, the highest ability to produce CLA from LA is Lac.02 strain. In the production of CLA from LA, enzymes involved in this metabolism in Lactobacillus act as catalysts of hydration/dehydration (CLA-HY), oxidation of hydroxy groups/reduction of oxo groups (CLA-DH), migration of carbon-carbon double bonds (CLA-DC), and saturation of carbon-carbon double bonds (CLA-ER). The cla-dh, cla-dc, cla-hy and cla-er genes that encode enzymes CLA-DH, CLA-DC, and CLA-ER had been found in all Lac.02, Lac.05, Lac.14 and Lac.16 strains. Gas chromatography traces indicated that these strains produced the same compounds, which was subsequently identified as cis-9, trans-11, and trans-10, cis-12 CLA. In the next study, we will optimize the conditions such as substrate concentrations, pH values, temperature and culture time of each strain to obtain the best rerults.

scholarly journals Influence of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids

2006 ◽  
Vol 95 (6) ◽  
pp. 1199-1211 ◽  
Author(s):  
I. Wąsowska ◽  
M. R. G. Maia ◽  
K. M. Niedźwiedzka ◽  
M. Czauderna ◽  
J. M. C. Ramalho Ribeiro ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Fish Oil ◽  
Unsaturated Fatty Acids ◽  
Butyrivibrio Fibrisolvens ◽  
Isomerase Activity ◽  
Epa And Dha ◽  
Pure Cultures ◽  
Ruminal Biohydrogenation

Dietarycis-9,trans-11-conjugated linoleic acid (CLA) is generally thought to be beneficial for human health. Fish oil added to ruminant diets increases the CLA concentration of milk and meat, an increase thought to arise from alterations in ruminal biohydrogenation of unsaturated fatty acids. To investigate the mechanism for this effect,in vitroincubations were carried out with ruminal digesta and the main biohydrogenating ruminal bacterium,Butyrivibrio fibrisolvens. Linoleic acid (LA) or α-linolenic acid (LNA) was incubated (1·67g/l) with strained ruminal digesta from sheep receiving a 50:50 grass hay–concentrate ration. Adding fish oil (up to 4·17g/l) tended to decrease the initial rate of LA (P=0·025) and LNA (P=0·137) disappearance, decreased (P<0·05) the transient accumulation of conjugated isomers of both fatty acids, and increased (P<0·05) the accumulation oftrans-11-18:1. Concentrations of EPA (20:5n-3) or DHA (22:6n-3), the major fatty acids in fish oil, were low (100mg/l or less) after incubation of fish oil with ruminal digesta. Addition of EPA or DHA (50mg/l) to pure cultures inhibited the growth and isomerase activity ofB. fibrisolvens, while fish oil had no effect. In contrast, similar concentrations of EPA and DHA had no effect on biohydrogenation of LA by mixed digesta, while the addition of LA prevented metabolism of EPA and DHA. Neither EPA nor DHA was metabolised byB. fibrisolvensin pure culture. Thus, fish oil inhibits ruminal biohydrogenation by a mechanism which can be interpreted partly, but not entirely, in terms of its effects onB. fibrisolvens.

Conjugated Linoleic Acid Production by Fermentation

2006 ◽  
Vol 2 (4) ◽  
Author(s):  
Ming Dong ◽  
Shuting Qi
Keyword(s):  
Linoleic Acid ◽  
Incubation Time ◽  
Lactobacillus Acidophilus ◽  
Seed Oil ◽  
Incubation Temperature ◽  
Conversion Ratio ◽  
Isomerase Activity ◽  
Whole Milk ◽  
Alfalfa Seed ◽  
Linoleic Acid Isomerase

Lactobacillus acidophilus 1.1854 was used for CLA production in whole milk and alfalfa seed oil was used as substrate. Alfalfa seed oil contained linoleic acid about 40%. Results showed that alfalfa seed oil addition to the culture improved CLA production, indicating the presence of linoleic acid isomerase activity in the culture. The concentration of lactic acid bacteria, the incubation time, the substrate concentration, the pH, incubation temperature, the pre-incubation time and the substrate amount of pre-incubation were studied in our research and they are optimized at 2.5%(v/v), 21h, 0.05%(v/v), pH 6.4, 37°C, 11h and 10µL which brought the optimal conversion ratio at about 50%.

Linoleic acid isomerization over mesoporous carbon supported gold catalysts

2010 ◽  
Vol 150 (1-2) ◽  
pp. 32-36 ◽  
Author(s):  
Olga A. Simakova ◽  
Anne-Riikka Leino ◽  
Betiana Campo ◽  
Päivi Mäki-Arvela ◽  
Krisztián Kordás ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Mesoporous Carbon ◽  
Gold Catalysts ◽  
Supported Gold Catalysts ◽  
Acid Isomerization ◽  
Supported Gold

Linoleic acid isomerization on Ru/Al2O3 catalyst

2005 ◽  
Vol 115 (1-2) ◽  
pp. 13-22 ◽  
Author(s):  
Andreas Bernas ◽  
Dmitry Yu. Murzin
Keyword(s):  
Linoleic Acid ◽  
Acid Isomerization ◽  
Al2o3 Catalyst

scholarly journals Conjugated linoleic acid producing potential of lactobacilli isolated from goat (AXB) rumen fluid samples

2020 ◽  
Vol 33 (8) ◽  
pp. 1233-1241
Author(s):  
Amrish Kumar Tyagi ◽  
Sachin Kumar ◽  
Prasanta Kumar Choudhury ◽  
Bhawna Tyagi ◽  
Nitin Tyagi
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Rumen Fluid ◽  
Spectrophotometric Assay ◽  
Lactobacillus Species ◽  
Rrna Gene ◽  
Chain Reaction ◽  
Specific Polymerase Chain Reaction ◽  
Polymerase Chain ◽  
Linoleate Isomerase

Objective: The present investigation was aimed to explore the potential of lactobacilli for conjugated linoleic acid (CLA) production, isolated from rumen fluid samples of lactating goats.Methods: A total of 64 isolates of lactobacilli were obtained using deMan-Rogosa-Sharpe (MRS) agar from rumen fluid of goats and further subjected to morphological and biochemical characterizations. Isolates found as gram-positive, catalase negative rods were presumptively identified as Lactobacillus species and further confirmed by genus specific polymerase chain reaction (PCR). The phylogenetic tree was constructed from the nucleotide sequences using MEGA6.Results: Out of the 64 isolates, 23 isolates were observed positive for CLA production by linoleate isomerase gene-based amplification and quantitatively by UV-spectrophotometric assay for the conversion of linoleic acid to CLA as well as gas chromatography-based assay. In all Lactobacillus species cis9, trans11 isomer was observed as the most predominant CLA isomer. These positive isolates were identified by 16S rRNA gene-based PCR sequencing and identified to be different species of <i>L. ingluviei</i> (2), <i>L.salivarius</i> (2), <i>L. curvatus</i> (15), and <i>L. sakei</i> (4).Conclusion: The findings of the present study concluded that lactic acid bacteria isolated from ruminal fluid samples of goat have the potential to produce bioactive CLA and may be applied as a direct fed microbial to enhance the nutraceutical value of animal food products.

scholarly journals PpoC from Aspergillus nidulans is a fusion protein with only one active haem

2010 ◽  
Vol 425 (3) ◽  
pp. 553-565 ◽  
Author(s):  
Florian Brodhun ◽  
Stefan Schneider ◽  
Cornelia Göbel ◽  
Ellen Hornung ◽  
Ivo Feussner
Keyword(s):  
Linoleic Acid ◽  
Aspergillus Nidulans ◽  
Octadecadienoic Acid ◽  
Terminal Region ◽  
Site Directed Mutagenesis ◽  
Isomerase Activity ◽  
Parallel Decomposition ◽  
Hydroxyoctadecadienoic Acid ◽  
Epoxy Alcohols ◽  
Hydroperoxyoctadecadienoic Acid

In Aspergillus nidulans Ppos [psi (precocious sexual inducer)-producing oxygenases] are required for the production of so-called psi factors, compounds that control the balance between the sexual and asexual life cycle of the fungus. The genome of A. nidulans harbours three different ppo genes: ppoA, ppoB and ppoC. For all three enzymes two different haem-containing domains are predicted: a fatty acid haem peroxidase/dioxygenase domain in the N-terminal region and a P450 haem-thiolate domain in the C-terminal region. Whereas PpoA was shown to use both haem domains for its bifunctional catalytic activity (linoleic acid 8-dioxygenation and 8-hydroperoxide isomerization), we found that PpoC apparently only harbours a functional haem peroxidase/dioxygenase domain. Consequently, we observed that PpoC catalyses mainly the dioxygenation of linoleic acid (18:2Δ9Z,12Z), yielding 10-HPODE (10-hydroperoxyoctadecadienoic acid). No isomerase activity was detected. Additionally, 10-HPODE was converted at lower rates into 10-KODE (10-keto-octadecadienoic acid) and 10-HODE (10-hydroxyoctadecadienoic acid). In parallel, decomposition of 10-HPODE into 10-ODA (10-octadecynoic acid) and volatile C-8 alcohols that are, among other things, responsible for the characteristic mushroom flavour. Besides these principle differences we also found that PpoA and PpoC can convert 8-HPODE and 10-HPODE into the respective epoxy alcohols: 12,13-epoxy-8-HOME (where HOME is hydroxyoctadecenoic acid) and 12,13-epoxy-10-HOME. By using site-directed mutagenesis we demonstrated that both enzymes share a similar mechanism for the oxidation of 18:2Δ9Z,12Z; they both use a conserved tyrosine residue for catalysis and the directed oxygenation at the C-8 and C-10 is most likely controlled by conserved valine/leucine residues in the dioxygenase domain.

scholarly journals Synthesis of conjugated linoleic acid by the linoleate isomerase complex in food-derived lactobacilli

2014 ◽  
Vol 117 (2) ◽  
pp. 430-439 ◽  
Author(s):  
B. Yang ◽  
H. Chen ◽  
Z. Gu ◽  
F. Tian ◽  
R. P. Ross ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Linoleate Isomerase
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