High-frequency plant regeneration by in vitro shoot proliferation in cotyledonary node explants of grasspea (Lathyrus sativus L.)

2004 ◽  
Vol 40 (5) ◽  
pp. 467-470 ◽  
Author(s):  
D. P. Barik ◽  
S. K. Naik ◽  
U. Mohapatra ◽  
P. K. Chand
2011 ◽  
Vol 21 (2) ◽  
pp. 135-141 ◽  
Author(s):  
A.K. M. Sayeed Hassan ◽  
Nadira Begum ◽  
Rebeka Sultana ◽  
Rahima Khatun

An efficient protocol was developed for shoot proliferation and plant regeneration of Phlogacanthus thyrsiflorus Nees. (Acanthaceae) - a rare medicinal shrub of Bangladesh, through in vitro culture using shoot tip and nodal explants. Best shoot induction was observed on MS with 1.0 mg/l BAP + 0.5 mg/l NAA, in which 84.2% of nodal explants responded to produce maximum number (12.4 ± 0.66) of shoots per culture. In vitro raised shoots rooted on half-strength MS with 0.5 mg/l IBA + 0.5 mg/l NAA. For acclimation and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%. Key words: Phlogacanthus thyrsiflorus, Shoot proliferation, Plant regeneration   D. O. I. 10.3329/ptcb.v21i2.10236   Plant Tissue Cult. & Biotech. 21(2): 135-141, 2011 (December)


2005 ◽  
Vol 49 (4) ◽  
pp. 637-639 ◽  
Author(s):  
D. P. Barik ◽  
U. Mohapatra ◽  
P. K. Chand

2017 ◽  
Vol 27 (1) ◽  
pp. 85-88 ◽  
Author(s):  
Mohsen Hesami ◽  
Mohammad Hosein Daneshvar ◽  
Amin Lotfi

Abstract not availablePlant Tissue Cult. & Biotech. 27(1): 85-88, 2017 (June)


2004 ◽  
Vol 44 (3) ◽  
pp. 108-110
Author(s):  
KG Prashantha ◽  
BN Sathyanarayana ◽  
Deepu Mathew

2011 ◽  
Vol 73 (1) ◽  
pp. 5-8 ◽  
Author(s):  
Anna Stojakowska ◽  
Janusz Malarz

A micropropagation method, through axillary shoot proliferation, was elaborated for <em>Inula royleana </em>DC. (Asteraceae), a medicinal plant native of Himalaya. Primary explants (cotyledonary node explants) and secondary explants (node explants of in vitro regenerated shoots) of the plant, inoculated on MS medium supplemented with 0.1 μM NAA and 5.0 μM kinetin, regenerated 3.4 ± 1.2 and 5.1 ± 1.9 axillary shoots per explant, respectively. The regenerated shoots were easily rooting and adapting to growth in soil.


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