Study on induced mutations in m1 generation in sorghum [Sorghum bicolor (L.) Moench ]

An investigation was carried out to create the variability generated through induced mutation in two sorghum populations viz., 296 B (Kharif) and Parbhani Moti (Rabi). Two mutagens viz., gamma irradiation (10 kR, 20kR, 30kR and 40kR) and EMS (0.1%EMS, 0.2%EMS, 0.3%EMS and 0.3%EMS) and their combination were used M1 generation. Mutagenic sensitivity in M1 generation on the basis of reduced germination and plant survival revealed a dose dependent reaction and differential response of the populations. LD50 was found to be 20-30 kR in case of gamma irradiation and 0.3-0.4 per cent in EMS irrespective of the genotype. The irradiated population produced more number of superior segregants in respects of seed yield and its contributing traits compared to other populations. Three dwarf mutant, one brown midrib and tree drought tolerance confirmed from Parbhani Moti.

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Study on mutagenic effectiveness and efficiency of mutagens in inducing chlorophyll mutations in m2 generation in sorghum [Sorghum bicolor (L.) Moench ]

INTERNATIONAL JOURNAL PLANT SCIENCES ◽

10.15740/has/ijps/15.2/107-112 ◽

2020 ◽

Vol 15 (2) ◽

pp. 107-112

Author(s):

S.M. Surashe ◽

H.V. Kalpande ◽

S.B. Borgaonkar

Keyword(s):

Gamma Irradiation ◽

Sorghum Bicolor ◽

Mutation Frequency ◽

Chlorophyll Mutation ◽

Chlorophyll Mutations ◽

Chlorophyll Mutants ◽

Mutagenic Sensitivity ◽

Effectiveness And Efficiency ◽

Dose Dependent ◽

Sorghum Bicolor L

An investigation was carried out to create the variability generated through induced mutation in two sorghum populations viz., 296 B (Kharif) and Parbhani Moti (Rabi). Two mutagens viz., gamma irradiation (10 kR, 20kR, 30kR and 40kR) and EMS (0.1%EMS, 0.2%EMS, 0.3%EMS and 0.3%EMS) and their combination were used M2 generation. Mutagenic sensitivity in M2 generation on the basis of reduced germination and plant survival revealed a dose dependent reaction and differential response of the populations. In general, chlorophyll mutation frequency expressed on M2 seedling basis increased linearly with doses of three the mutagens in 296 B and Parbhani Moti. The frequency was more in 296 B followed by Parbhani Moti of three mutagens. The spectrum of chlorophyll mutations comprised albina, xantha, viridis, xanthaviridis, chlorina. The most frequently occurred mutant was viridis type followed by chlorina in all the populations. The population 296 B had expressed largest frequency of chlorophyll mutants followed by Parbhani Moti.

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PENGARUH INOKULAN BAKTERI ASAM LAKTAT DAN ADITIF TERHADAP KUALITAS DAN KARAKTERISTIK SILASE SORGUM MUTAN 2 1 BROWN MIDRIB (Sorghum bicolor L. Moench)

Pastura ◽

10.24843/pastura.2019.v09.i01.p11 ◽

2019 ◽

Vol 9 (1) ◽

pp. 40

Author(s):

R. Sriagtula ◽

I. Martaguri ◽

J. Hellyward ◽

S. Sowmen

Keyword(s):

Sorghum Bicolor ◽

Brown Midrib ◽

Duncan Multiple Range Test ◽

Sorghum Bicolor L ◽

Range Test

Penelitian ini bertujuan untuk mengobservasi pengaruh penambahan inokulasi bakteri asam laktat (BAL) dan aditif terhadap kualitas dan karakterietik silase whole crop sorgum mutan brown midrib (Sorghum bicolor L. Moench) galur Patir 3.7 yang dipanen pada fase soft dough. Penelitian dilaksanakan secara eksperimen menggunakan rancangan acak lengkap pola faktorial dengan 4 ulangan. Faktor A yaitu A1 = tanpa BAL, A2= penambahan BAL. Faktor B terdiri dari B1= tanpa aditif, B2= dedak, B3= jagung. Sumber BAL yang digunakan berasal dari inokulan komersil dari minuman fermentasi merk Yakult dengan dosis 1 ml (v/w) atau 11×109 CFU/ml/berat segar. Aditif terdiri dari dedak padi dan jagung halus digunakan sebanyak 3% (g/g)/berat segar. Parameter yang diamati adalah karakteristik dan kualitas silase meliputi nilai pH, nilai fleigh (NF), kandungan bahan kering (BK), protein kasar (PK), serat kasar (SK), lemak kasar (LK) dan Abu. Data dianalisis berdasarkan analisis keragaman menurut Duncan Multiple Range Test (DMRT). Hasil penelitian menunjukkan bahwa tidak terdapat interaksi (P>0,05) antara penambahan BAL dan aditif terhadap pH, NF, BK, PK, SK, LK dan abu, sedangkan faktor tunggal adititif memberikan pengaruh berbeda nyata (P<0,05) lebih tinggi terhadap kandungan BK silase whole crop sorgum mutan BMR. Dari penelitian ini dapat disimpulkan bahwa secara umum penambahan inokulan BAL dan aditif menghasilkan karakteristik dan kualitas silase yang sama, namun demikian penambahan dedak padi dan jagung halus menghasilkan BK silase yang lebih tinggi dibanding tanpa BAL dan aditif. Kata kunci: aditif, BAL, brown midrib, silase, sorgum

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