scholarly journals Evidence of Low Chloroplast Genetic Diversity in Two Carpinus Species in the Northern Balkans

2017 ◽  
Vol 45 (1) ◽  
pp. 316-322
Author(s):  
Mihaela Cristina CĂRĂBUŞ ◽  
Alexandru Lucian CURTU ◽  
Dragoș POSTOLACHE ◽  
Elena CIOCÎRLAN ◽  
Neculae ŞOFLETEA
Keyword(s):  
Genetic Diversity ◽  
Peripheral Population ◽  
Northern Limit ◽  
Natural Distribution ◽  
Simple Sequence Repeat Markers ◽  
Distinct Haplotype ◽  
Flow Through ◽  
Low Levels ◽  
Chloroplast Haplotypes ◽  
Simple Sequence

Genetic diversity and differentiation in two Carpinus species (C. betulus and C. orientalis) occurring in Romania was investigated by using three chloroplast Simple Sequence Repeat markers (cpSSRs). A total of 96 and 32 individuals were sampled in eighteen C. betulus and six C. orientalis populations, respectively. A total of four chloroplast haplotypes were observed. Two haplotypes were specific for C. betulus and two for C. orientalis. Most of C. betulus populations were fixed for the predominant haplotype (H1), which was observed in 82% of the individuals. All C. orientalis populations were fixed for one haplotype or the other. Populations with haplotype (H3) are spread in southern Romania and the haplotype (H4) was observed at the northern limit of C. orientalis natural distribution range. Genetic differentiation among populations was moderate in C. betulus (GST = 0.422), compared to the high value observed in C. orientalis (GST = 1.000), which can be explained by the occurrence of a distinct haplotype in the peripheral population. RST values for both species suggest low levels of recurrent gene flow through seeds among populations. Our data on geographic distribution of chloroplast DNA haplotypes may be useful for the identification and conservation of distinct genetic resources of the two Carpinus species.

scholarly journals Genetic diversity among Puccinia melanocephala isolates from Brazil assessed using simple sequence repeat markers

2014 ◽  
Vol 13 (3) ◽  
pp. 7852-7863 ◽  
Author(s):  
R.F. Peixoto-Junior ◽  
S. Creste ◽  
M.G.A. Landell ◽  
D.S. Nunes ◽  
A. Sanguino ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeat ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
Puccinia Melanocephala ◽  
Simple Sequence

scholarly journals Genetic diversity of Pongamia pinnata in Bali, Indonesia using Inter Simple Sequence Repeat markers

2019 ◽  
Vol 20 (8) ◽  
Author(s):  
Ni Luh Arpiwi ◽  
I Gusti Ayu Sugi Wahyuni ◽  
I Ketut Muksin
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Simple Sequence Repeat ◽  
Inter Simple Sequence Repeat ◽  
Pongamia Pinnata ◽  
Sequence Repeat ◽  
Binary Matrix ◽  
Simple Sequence Repeat Markers ◽  
Group I ◽  
Simple Sequence

Abstract. Arpiwi NL, Wahyuni IGAS, Muksin IK. 2019. Genetic diversity of Pongamia pinnata in Bali, Indonesia using Inter Simple Sequence Repeat markers. Biodiversitas 20: 2134-2142. Pongamia pinnata (L.) Pierre is a member of family Leguminosae that produces seed oil for biodiesel feedstock. The aim of the present study was to determine genetic diversity of pongamia trees that grow in Bali using Inter Simple Sequence Repeat (ISSR) markers. This study is important to support the breeding program for the improvement of the biodiesel producing species. Leaf samples were taken from 26 pongamia trees grown on northern and southern coastal areas of Bali. Genomic DNA was isolated from fresh leaves sample and was amplified by Polymerase Chain Reaction (PCR) using 9 ISSR primers. The banding patterns of DNA after PCR were scored and tabulated into a binary matrix. Genetic distance was generated by pairwise distance using composite maximum likelihood. A dendrogram was constructed using Unweighted Pair Group Method Arithmetic (UPGMA) method. The binary matrix was further analyzed for Nonmetric Multidimensional Scaling (NMDS) with Primer E V.6 software. DNA concentrations ranged from 98.59-100.55 ng/μL with sufficient quality for PCR. The number of alleles for 9 primers was 43, the number of the polymorphic band was 35, and the number of monomorphic bands was 8. Percentage of polymorphism ranged from 50 to 100%. Cluster analysis of 26 DNA of pongamia trees showed that the trees were grouped into two, namely group I and II. Group I consisted of two trees only, namely Uma Anyar 1 and Penarukan 1. Group II consisted of 24 pongamia trees which were divided into 3 subgroups, namely IIA, IIB, and IIC with close genetic distance. Analysis of NMDS supported cluster analysis that 23 out of 26 pongamia trees had close genetic distance, and possibly they come from a similar source. Genetic diversity of pongamia in Bali needs to be widen possibly by the introduction of new planting materials from across Indonesia or seed procurement from different sources.

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