scholarly journals Concurrent detection of five Yellow dwarf viruses (B/CYDVs) in wheat in Mardin (Turkey) and phylogenetic relationship of BYDV-PAV

2020 ◽  
Vol 48 (4) ◽  
pp. 1862-1872
Author(s):  
Abdulselam KARAOZAN ◽  
Mustafa USTA
Keyword(s):  
Phylogenetic Relationship ◽  
Infection Rate ◽  
Economic Loss ◽  
Triticum Aestivum L ◽  
Yellow Dwarf ◽  
Survey Study ◽  
Virus Species ◽  
Wheat Production ◽  
Production Areas ◽  
Relationship Of

Wheat (Triticum aestivum L.) is a natural host of many viruses. Yellow dwarf viruses belonging to the Luteoviridae family are important virus species that cause economic loss by restricting wheat production worldwide. Surveys were conducted in 2017 to determine Yellow dwarf viruses (BYDV-PAV, BYDV-MAV, BYDV-SGV, BYDV-RMV, and CYDV-RPV) and their infection rates in wheat production areas in Mardin province. 400 fresh leaf samples collected were tested by  Multiplex reverse transcription-polymerase chain reaction (m-RT-PCR). The overall infection rate was found to be 3%. BYDV-PAV has been identified as the most widespread virus with a 2.5% presence rate. It was found out that BYDV-SGV, CYDV-RPV, and BYDV-RMV infections were lower, with rates of 1.75%, 0.5% and 0.25% respectively. In the current study, double infections were detected in 8 samples. The overall infection rate of the detected viruses (BYDV-PAV, BYDV-SGV, CYDV-RPV, BYDV-RMV) was found to be lower than the records reported in previous similar studies. No BYDV-MAV infection was found in any of the wheat samples tested. The cDNA of the coat protein (CP) gene of a BYDV-PAV isolate randomly selected from virus-positive samples was cloned, bidirectionally sequenced, and the phylogenetic relationship revealed. According to the phylogenetic analysis with 19 different isolates in the NCBI database of BYDV-PAV Mardin isolate, it showed the highest genetic similarity by 95.52% with the Germany isolate (KY634926) while the lowest similarity rate was 89.22% with the Germany and Pakistan isolates (KY634886 and JQ811489). The presence of BYDV-PAV, BYDV-SGV, CYDV-RPV, and BYDV-RMV were reported for the first time with this survey study conducted in Mardin.

scholarly journals Cloning, characterization of TaGS3 gene and identification of allelic variation associated with kernel traits in wheat (Triticum aestivum L.)

2019 ◽  
Author(s):  
Jian Yang ◽  
Yanjie Zhou ◽  
Yu'e Zhang ◽  
Weiguo Hu ◽  
Qiuhong Wu ◽  
...  
Keyword(s):  
Allelic Variation ◽  
Triticum Aestivum L ◽  
Wheat Breeding ◽  
Allelic Frequency ◽  
Yield Component ◽  
Grain Weight ◽  
Specific Gene ◽  
Wheat Production ◽  
Trait Locus ◽  
Production Areas

Abstract Background: Grain weight is an important yield component. Selection of advanced lines with heavy grains show high grain sink potentials and strong sink activity, which is an increasingly important objective in wheat breeding programs. Rice OsGS3 has been identified as a major quantitative trait locus for both grain weight and grain size. However, allelic variation of GS3 has not been characterized previously in hexaploid wheat. Results : We cloned 2445, 2393, and 2409 bp sequences of the homologs TaGS3-4A , TaGS3-7A , and TaGS3-7D in wheat ‘Changzhi 6406’, a cultivar that shows high grain weight. The TaGS3 genes each contained five exons and four introns, and encoded a deduced protein of 170, 169, and 169 amino acids, respectively. Phylogenetic analysis of plant GS3 protein sequences revealed GS3 to be a monocotyledon-specific gene and the GS3 proteins were resolved into three classes. The length of the atypical Gγ domain and the cysteine-rich region was conserved within each class and not conserved between classes. A single-nucleotide polymorphism in the fifth exon (at position 1907) of TaGS3-7A leads to an amino acid change (ALA/THR) and showed different frequencies in two pools of Chinese wheat accessions representing extremes in grain weight. Association analysis indicated that the TaGS3-7A-A allele was associated with higher grain weight in the natural population. The TaGS3-7A-A allele was favoured in global modern wheat cultivars but the allelic frequency varied among different wheat-production regions of China, which indicated that this allele is of potential utility to improve wheat grain weight in certain wheat-production areas of China. Conclusions : The novel molecular information on wheat GS3 homologs and the KASP functional marker designed in this study may be useful in marker-assisted breeding for genetic improvement of wheat.

scholarly journals Cloning, characterization of TaGS3 gene and identification of allelic variation associated with kernel traits in wheat (Triticum aestivum L.)

2019 ◽  
Author(s):  
Jian Yang ◽  
Yanjie Zhou ◽  
Yu'e Zhang ◽  
Weiguo Hu ◽  
Qiuhong Wu ◽  
...  
Keyword(s):  
Allelic Variation ◽  
Triticum Aestivum L ◽  
Wheat Breeding ◽  
Allelic Frequency ◽  
Yield Component ◽  
Grain Weight ◽  
Specific Gene ◽  
Wheat Production ◽  
Trait Locus ◽  
Production Areas

Abstract Background: Grain weight is an important yield component. Selection of advanced lines with heavy grains show high grain sink potentials and strong sink activity, which is an increasingly important objective in wheat breeding programs. Rice OsGS3 has been identified as a major quantitative trait locus for both grain weight and grain size. However, allelic variation of GS3 has not been characterized previously in hexaploid wheat. Results : We cloned 2445, 2393, and 2409 bp sequences of the homologs TaGS3-4A , TaGS3-7A , and TaGS3-7D in wheat ‘Changzhi 6406’, a cultivar that shows high grain weight. The TaGS3 genes each contained five exons and four introns, and encoded a deduced protein of 170, 169, and 169 amino acids, respectively. Phylogenetic analysis of plant GS3 protein sequences revealed GS3 to be a monocotyledon-specific gene and the GS3 proteins were resolved into three classes. The length of the atypical Gγ domain and the cysteine-rich region was conserved within each class and not conserved between classes. A single-nucleotide polymorphism in the fifth exon (at position 1907) of TaGS3-7A leads to an amino acid change (ALA/THR) and showed different frequencies in two pools of Chinese wheat accessions representing extremes in grain weight. Association analysis indicated that the TaGS3-7A-A allele was associated with higher grain weight in the natural population. The TaGS3-7A-A allele was favoured in global modern wheat cultivars but the allelic frequency varied among different wheat-production regions of China, which indicated that this allele is of potential utility to improve wheat grain weight in certain wheat-production areas of China. Conclusions : The novel molecular information on wheat GS3 homologs and the KASP functional marker designed in this study may be useful in marker-assisted breeding for genetic improvement of wheat.

scholarly journals Cloning, characterization of TaGS3 and identification of allelic variation associated with kernel traits in wheat (Triticum aestivum L.)

BMC Genetics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Jian Yang ◽  
Yanjie Zhou ◽  
Yu’e Zhang ◽  
Weiguo Hu ◽  
Qiuhong Wu ◽  
...  
Keyword(s):  
Allelic Variation ◽  
Triticum Aestivum L ◽  
Wheat Breeding ◽  
Allelic Frequency ◽  
Yield Component ◽  
Grain Weight ◽  
Specific Gene ◽  
Wheat Production ◽  
Trait Locus ◽  
Production Areas

Abstract Background Grain weight is an important yield component. Selection of advanced lines with heavy grains show high grain sink potentials and strong sink activity, which is an increasingly important objective in wheat breeding programs. Rice OsGS3 has been identified as a major quantitative trait locus for both grain weight and grain size. However, allelic variation of GS3 has not been characterized previously in hexaploid wheat. Results We cloned 2445, 2393, and 2409 bp sequences of the homologs TaGS3-4A, TaGS3-7A, and TaGS3-7D in wheat ‘Changzhi 6406’, a cultivar that shows high grain weight. The TaGS3 genes each contained five exons and four introns, and encoded a deduced protein of 170, 169, and 169 amino acids, respectively. Phylogenetic analysis of plant GS3 protein sequences revealed GS3 to be a monocotyledon-specific gene and the GS3 proteins were resolved into three classes. The length of the atypical Gγ domain and the cysteine-rich region was conserved within each class and not conserved between classes. A single-nucleotide polymorphism in the fifth exon (at position 1907) of TaGS3-7A leads to an amino acid change (ALA/THR) and showed different frequencies in two pools of Chinese wheat accessions representing extremes in grain weight. Association analysis indicated that the TaGS3-7A-A allele was associated with higher grain weight in the natural population. The TaGS3-7A-A allele was favoured in global modern wheat cultivars but the allelic frequency varied among different wheat-production regions of China, which indicated that this allele is of potential utility to improve wheat grain weight in certain wheat-production areas of China. Conclusions The novel molecular information on wheat GS3 homologs and the KASP functional marker designed in this study may be useful in marker-assisted breeding for genetic improvement of wheat.

Morphological re-description and phylogenetic relationship of five myxosporean species of the family Myxobolidae infecting Nile tilapia

2017 ◽  
Vol 124 (3) ◽  
pp. 201-214 ◽  
Author(s):  
R Abdel-Gaber ◽  
F Abdel-Ghaffar ◽  
S Maher ◽  
AM El-Mallah ◽  
S Al Quraishy ◽  
...  
Keyword(s):  
Phylogenetic Relationship ◽  
Nile Tilapia ◽  
The Family ◽  
Relationship Of ◽  
Myxosporean Species

Phylogenetic Relationship of Ramie and Its Wild Relatives Based on Cytoge-netics and DNA Sequences

2009 ◽  
Vol 35 (10) ◽  
pp. 1778-1790
Author(s):  
Liang LIAO ◽  
Tong-Jian LI ◽  
Zhong-Lai LIU ◽  
Hui-Sheng DENG ◽  
Ling-Ling XU ◽  
...  
Keyword(s):  
Phylogenetic Relationship ◽  
Dna Sequences ◽  
Wild Relatives ◽  
Relationship Of

Kuwaiti Hospital Patients’ Continuance Intention to Use Telemedical Systems in the Wake of the COVID-19 Pandemic: Survey Study Among Patients (Preprint)

2021 ◽  
Author(s):  
Ibtesam Almutairi
Keyword(s):  
Service Quality ◽  
Information Quality ◽  
Survey Study ◽  
Intention To Use ◽  
System Quality ◽  
Continuance Intention ◽  
Quantitative Research Methods ◽  
Patient’S Satisfaction ◽  
Relationship Of ◽  
The Relationship

BACKGROUND Telemedicine is a system using telecommunication technologies to diagnose, treat, and monitor patients by healthcare physicians and specialists in many developing countries such as Kuwait. Telemedicine services have proven to be successful in reporting and tracking patient records, delivering, real time monitoring, providing correct medications, and early detection of clinical decline. Covid-19 pandemic period have reinforced telemedicine system’s benefits even more in Kuwait. OBJECTIVE The objective of this study is to investigate factors influencing patients’ continuance intention to use telemedicine after the COVID-19 pandemic in the medical sector of Kuwait. METHODS The updated Delone and Maclean (2003) model was utilized to investigate the aforementioned factors. As such, this research applied quantitative research methods with a sample of 290 participants from patients in Dar Al Shifa Hospital, a private hospital in Kuwait which utilizes telemedical services called ‘Sehaty online’. The corresponding data was analyzed using SmartPLS. RESULTS The findings of this study revealed that the relationship of both telemedicine’s information quality and system quality with patient’s satisfaction are significant with (β = 0.377, t = 5.612, P < 0.001), (β = 0.295, t = 4.397, P < 0.001) respectively. While the relationship of service quality and patient’s satisfaction is not significant with (β = -0.056, t = 0.894, P > 0.05). patient’s satisfaction relationship with patients’ continuance intention to use telemedicine found to be significant with (β = 0.403, t = 8.732, P < 0.001). CONCLUSIONS It has been concluded that information quality and system quality have a positive and significant influence on patient’s satisfaction, whereas service quality has an insignificant influence on patient’s satisfaction. Also, patients’ continuance intention to use telemedicine is found to be significantly impacted by their satisfaction.

Phylogenetic Relationship of the Greater White-Fronted GooseAnser albifronsSubspecies Wintering in the Palaearctic Region

2013 ◽  
Vol 12 (1) ◽  
pp. 35-42 ◽  
Author(s):  
Masaki Eda ◽  
Tetsuo Shimada ◽  
Tatsuya Amano ◽  
Katsumi Ushiyama ◽  
Chitoshi Mizota ◽  
...  
Keyword(s):  
Phylogenetic Relationship ◽  
Palaearctic Region ◽  
Relationship Of
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