scholarly journals Application of Chondroitin Sulfate on Organogenesis of Two Cymbidium spp. under Different Sources of Lights

2016 ◽  
Vol 8 (2) ◽  
pp. 156-160 ◽  
Author(s):  
Syeda Jabun NAHAR ◽  
Syed M. HAQUE ◽  
Shimasaki KAZUHIKO

The aim of this study was to present chondroitin sulfate as a plant growth regulator and to give an overview about light effects on PLBs (protocorm like bodies) culture of Cymbidium dayanum and Cymbidium finlaysonianum cultured in vitro. Chondroitin sulfate is a sulfated glycosaminoglycan (GAG) composed of a chain of alternating sugars N-acetylgalactosamine and glucuronic acid. It is widely used as a material for food ingredients, cosmetics and medicine. PLBs were cultured on modified MS medium containing different concentration of chondroitin sulfate (0, 0.1, 1 and 10 mg/l), under four sources of lights: conventional white fluorescent tube, red LED, green LED and blue LED. In C. dayanum, 100% PLBs formation rate was observed at 0.1 mg/l chondroitin sulfate with modified MS medium under green LED and 1 mg/l chondroitin sulfate under blue LED; the maximum shoots and roots formation were observed under green LEDs (93% and 80% respectively) when media contained 0.1 mg/l chondroitin sulfate. In C. finlaysonianum, every concentrations of chondroitin sulfate enhanced the growth rate of PLBs when compared to control treatment, under all four sources of lights. The highest values were recorded with 0.1 mg/l chondroitin sulfate which induced 100% PLBs formation under blue LED, while 10 mg/l chondroitin sulfate had induced 100% PLBs formation under green LED. The highest percentage of shoots (73%) was initiated in the medium containing 10 mg/l chondroitin sulfate under green LED. Plant development was strongly influenced by the light quality and plant growth regulator functions as chemical messengers for intercellular communication of plant. The results demonstrated that low concentrations of chondroitin sulfate could promote PLBs, shoots and roots formation of Cymbidium spp. under green and blue LED.

2021 ◽  
Vol 20 (5) ◽  
pp. 119-126
Author(s):  
Burcu Tuncer

Rheum ribes L. is a perennial wild species. Young shoots and flower bunches are freshly consumed, and root and rhizomes are generally used for medicinal purposes. The aim of the present study was to improve the callus proliferation protocol for R. ribes L. under in vitro conditions. For callus induction, hypocotyl explants taken from 14-day old plantlets germinated in Murashige and Skoog (MS) media were cultured in MS media with 9 plant growth regulator (PGR) combinations containing 6-benzylaminopurine (BAP) (2, 3, and 4 mg/L) + naphthylacetic acid (NAA) (0.1, 0.5, and 1 mg/L). Then, for callus proliferation, 4 PGR combinations containing NAA (0.2 mg/L) + thidiazuron (TDZ) (0.5, 1, 2, and 3 mg) were used in the first set of experiments, and 36 PGR combinations containing BAP (1, 2, 3, and 4 mg/L) + indole-3-butyric acid (IBA) (0.2, 0.5, and 1 mg/L), BAP (1, 2, 3, and 4 mg/L) + NAA (0.2, 0.5, and 1 mg/L), and TDZ (1, 2, 3, and 4 mg/L) + NAA (0.2, 0.5, and 1 mg/L) were used in the second set of experiments. At the end of the second set of experiments, the greatest callus regeneration ratios were obtained due to the combinations including BAP and IBA as well as the low-dose TDZ- (especially 1 mg/L) and NAA- (0.2, 0.5, 1 mg/L) combinations. Regarding callus fresh weights, TDZ + NAA combinations were found to be more successful. The greatest callus fresh weight (12.7 ±0.4 g) was obtained from MS medium supplemented with 2 mg/L TDZ and 0.2 mg/L NAA.


2007 ◽  
Vol 35 (2) ◽  
pp. 993-996 ◽  
Author(s):  
Sulejman Redžepović ◽  
Sanja Sikora ◽  
Josip Čolo ◽  
Mihaela Blažinkov ◽  
Marija Pecina

2004 ◽  
Vol 44 (3) ◽  
pp. 343 ◽  
Author(s):  
A. C. Rath ◽  
A. J. Prentice

The effects ReTain Plant Growth Regulator (830 g in 1000 L water/ha) applied to a commercial block of 'Arctic Snow' nectarines 7 days before the first harvest was assessed in relation to harvest delay, fruit size, fruit quality (flesh firmness and brix), as well as the quality of the fruit following export to Taiwan. The untreated block (0.87 ha) and the ReTain-treated block (1.0 ha) were harvested according to the orchard's standard maturation criteria of background colour, size and percent blush colour. Based on picking schedules, the maturation of the ReTain block was delayed by 2.75 days (P<0.05). The fruit continued to grow during this delay and resulted in a 12.3% increase in yield and an increase of 393 packed cartons/ha. There were more (P<0.001) cartons of ReTain-treated fruit packed in grade sizes 69–78 mm diameter and less of smaller size classes (51–66 mm) compared with untreated fruit. The increase in both the number of cartons of class 1 fruit and fruit size on the ReTain block resulted in a Return on Investment (ROI) of 14.7 : 1 with an additional net return of AU$17 655/ha over the untreated block. There was a correlation between fruit size and flesh firmness with larger fruit being firmer than smaller fruit (P<0.001), however, packed ReTain-treated fruit was 3.2 N firmer on average (P<0.001) than packed untreated fruit for all size grades and from all harvests and packs. Brix levels increased (P<0.001) with fruit size but there was no difference (P = 0.568) between ReTain-treated fruit and untreated fruit. Two containers of fruit (early and late picks) were exported to Taiwan. The containers held a temperature of 0.5–2.0°C for the 14–15 day transit time from Australia to Taiwan. ReTain-treated fruit in both containers was 1.7 N firmer (P = 0.022) than untreated fruit upon arrival in Taiwan. After ambient storage (23°C) of fruit from container 1 for 45 h, fruit firmness declined (P<0.001) from an average of 65.7 to 43.2 N and there was no difference (P = 0.826) between treated or untreated fruit. It is concluded that ReTain application to 'Arctic Snow' nectarines can increase financial returns to growers through increased fruit size and quality benefits as well as maintain a higher flesh firmness following export.


Crop Science ◽  
2018 ◽  
Vol 58 (4) ◽  
pp. 1801-1807 ◽  
Author(s):  
E. H. Reasor ◽  
J. T. Brosnan ◽  
J. P. Kerns ◽  
W. J. Hutchens ◽  
D. R. Taylor ◽  
...  

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