scholarly journals In vitro growth and leaf anatomy of Cattleya walkeriana (Gardner, 1839) grown in natural ventilation system

Revista CERES ◽  
2014 ◽  
Vol 61 (6) ◽  
pp. 883-890 ◽  
Author(s):  
Adriano Bortolotti da Silva ◽  
Poliana Patrícia Lima ◽  
Livia Emanuelle Simao de Oliveira ◽  
Andre Luis Moreira
Keyword(s):  
Plant Growth ◽  
Leaf Anatomy ◽  
Natural Ventilation ◽  
Culture Medium ◽  
Ventilation System ◽  
Leaf Tissue ◽  
Conventional Ventilation ◽  
In Vitro Growth ◽  
Leaf Mesophyll

Natural ventilation system facilitates gaseous exchanges in in vitro plants promoting changes in the leaf tissue, which can be evaluated through the leaf anatomy, and it allows a cultivation closer to the photoautrophic micropropagation. The objective of this work was to evaluate the effects on in vitro growth and on the leaf anatomy of Cattleya walkeriana grown in natural and conventional ventilation system with different concentrations of sucrose (0; 15; 30 and 45 L-1) combined with different cultivation systems (conventional micropropagation and natural ventilation system). The culture medium was composed of MS salts, solidified with 7 g L-1 of agar and pH adjusted to 5.8. Forty milliliters of culture medium were distributed in 250 mL flasks, autoclaved at 120 ºC for 20 minutes. The greater plant growth, as well as the greater thickness of the mesophyll was observed with the use of 20 g L-1 sucrose in natural ventilation system. Plants grown in natural ventilation system showed a thicker leaf mesophyll, which is directly related to photoautotrophic crops. The natural ventilation system induced more elliptical stomata and probably more functional formats.

scholarly journals In vitro growth of Cattleya nobilior Rchb. f.: culture media, sealing systems and irradiance

2021 ◽  
Vol 51 ◽  
Author(s):  
Kaliana Gottschalk de Freitas ◽  
José Carlos Sorgato ◽  
Jackeline Schultz Soares ◽  
Luan Marlon Ribeiro
Keyword(s):  
Plant Height ◽  
Natural Ventilation ◽  
Culture Medium ◽  
Culture Media ◽  
Ventilation System ◽  
Light Condition ◽  
Leaf Length ◽  
In Vitro Growth ◽  
Randomized Design

ABSTRACT Some factors may influence the in vitro culture of native orchids, especially the culture medium, sealing system and light condition. This study aimed to evaluate the addition of banana pulp and peel in the formulation of culture media, sealing system and irradiance, in the in vitro growth of Cattleya nobilior Rchb. f. A completely randomized design was used in a factorial scheme with five culture media [Murashige & Skoog supplemented or not with banana pulp or peel (100 or 200g L-1)], two light conditions [3,000 K LED lamps (43 µmol m-2 s-1 or 86 µmol m-2 s-1)] and two sealing systems (natural and conventional ventilation), with six replicates of one vial each. The plant height, diameter of the largest pseudobulb, length of the largest root and of the largest leaf, fresh weight and number of leaves, roots and shoots were evaluated. The use of both the banana pulp and peel as additives in the culture medium formulation promoted the C. nobilior growth. The natural ventilation system increased the plant height, pseudobulb diameter and leaf length, while the conventional system promoted tillering.

scholarly journals In vitro Dendrobium nobile plant growth and rooting in different sucrose concentrations

2004 ◽  
Vol 22 (4) ◽  
pp. 780-783 ◽  
Author(s):  
Ricardo T. de Faria ◽  
Fabiana N. Rodrigues ◽  
Luciana do V.R. Oliveira ◽  
Cláudio Müller
Keyword(s):  
Plant Growth ◽  
Culture Medium ◽  
Culture Media ◽  
Sucrose Concentration ◽  
Ms Medium ◽  
In Vitro Growth ◽  
Dendrobium Nobile ◽  
Modified Ms Medium ◽  
Dendrobium Nobile Lindl

Sucrose is a very important component in in vitro culture media, serving as a source of carbon and energy. In this paper, the rooting and in vitro growth of Dendrobium nobile Lindl (Orchidaceae) were studied using different sucrose concentrations (0 g L-1; 5 g L-1; 10 g L-1; 20 g L-1; 30 g L-1 and 60 g L-1), in a modified MS medium containing half the regular concentration of macronutrients at pH 5.8. Greater increases in plant height (4.21±0.6 cm) and high seedling multiplication (1:4) were observed in the 60 g L-1 sucrose treatment, even without the addition of plant hormones. Sucrose concentration in the culture medium did not influence in vitro plant rooting.

scholarly journals Effects of exogenous proline and a natural ventilation system on the in vitro growth of orchids

2016 ◽  
Vol 32 (3) ◽  
pp. 619-626
Author(s):  
Adriano Bortolotti da Silva ◽  
Caroline Oliveira dos Reis ◽  
Jairo Osvaldo Cazetta ◽  
Samira Domingues Carlin ◽  
Paulo Roberto Corrêa Landgraf ◽  
...  
Keyword(s):  
Natural Ventilation ◽  
Ventilation System ◽  
In Vitro Growth ◽  
Exogenous Proline

scholarly journals Effects of Sucrose and Other Additives on In Vitro Growth and Development of Purple Coneflower (Echinacea purpurea L.)

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Dahanayake Nilanthi ◽  
Yue-Sheng Yang
Keyword(s):  
Plant Growth ◽  
Culture Medium ◽  
Respiratory Infections ◽  
Shoot Multiplication ◽  
Echinacea Purpurea ◽  
Naphthaleneacetic Acid ◽  
Acid Plant ◽  
Purple Coneflower ◽  
Upper Respiratory

Echinacea purpurea (purple coneflower) is being used for the preparation of more than 240 extracts, salves, and tinctures to help cure diseases like rabies, cold, and upper respiratory infections. Hence, efforts were made to develop a culture medium for successful in vitro culturing of cornflower and to regenerate buds and induce roots to enable mass propagation of selected clones. Of the three levels of sucrose tested as a supplement to MS media (Murashige and Skoog’s medium, 1962) 3% showed better rooting of buds and appeared morphologically normal and identical as compared to those grown at higher and lower concentrations (2 and 4%). The additives hydrolyzed lactabumin (0.0, 100, 300, and 900 mgL−1), peptone (0.0, 100, 300, and 900 mgL−1), and yeast (0.0, 100, 300, and 900 mgL−1) to media containing 0.3 mgL−1 BA (6-benzyladenine) and 0.01 mgL−1 NAA (naphthaleneacetic acid-plant growth regulators) has negatively influenced proliferation of shoots. The higher concentrations of the above have delayed the development of plantlets. Shoot multiplication was enhanced by coconut water with 2% being the best among 4 and 8% tested. Shoot organogenesis was not influenced by copper sulphate (0, 1.5, 3, 6, and 12 mgL−1) and silver nitrate (0.0, 0.5, 2.5, and 12.5 mgL−1) supplements and at higher concentrations of the above inhibited plant growth.

scholarly journals Carbohydrates on the growth of Cattleya schilleriana Reichb.f. seedlings (Orchidaceae)

2018 ◽  
Vol 48 (7) ◽  
Author(s):  
Renato Fernandes Galdiano Júnior ◽  
Cibele Mantovani ◽  
Eliana Gertrudes de Macedo Lemos
Keyword(s):  
Survival Data ◽  
Culture Medium ◽  
Seedling Survival ◽  
In Vitro Growth ◽  
Growth Data ◽  
Carbohydrate Source ◽  
Carbohydrate Supplementation ◽  
Percentage Survival ◽  
Half Strength

ABSTRACT: The aim of the present study was to evaluate the effects of carbohydrate supplementation on the propagation of the orchid Cattleya schilleriana. The 120-d-old seedlings were subcultured in fructose-, glucose-, or sucrose-supplemented (0, 15, 30, and 45g L-1) ½ MS culture medium (half-strength macronutrient concentrations), using a completely random design with four repetitions per treatment. After 120d of treatment, root number and length, leaf number and length, and fresh weight were evaluated, and seedling survival was evaluated after 75d of acclimatization in a greenhouse. The in vitro growth data were submitted to regression analysis, whereas the percentage survival data were analyzed using ANOVA and Tukey’s test. Both in vitro growth and ex vitro survival were lowest when the plantlets were grown in the absence of a carbohydrate source and highest (>90% survival) when supplemented with glucose. According to our findings, the addition of either glucose (30g L-1) or sucrose (30g L-1) is recommended for mass propagation of C. schilleriana.

scholarly journals Morphophysiological responses of Billbergia zebrina Lindl. (Bromeliaceae) in function of types and concentrations of carbohydrates during conventional in vitro culture

2020 ◽  
Vol 26 (1) ◽  
pp. 18-34 ◽  
Author(s):  
Elizangela Rodrigues Santos ◽  
João Paulo Rodrigues Martins ◽  
Luiz Carlos de Almeida Rodrigues ◽  
Andreia Barcelos Passos Lima Gontijo ◽  
Antelmo Ralph Falqueto
Keyword(s):  
Photosystem Ii ◽  
In Vitro Culture ◽  
Leaf Anatomy ◽  
Culture Medium ◽  
Stomatal Density ◽  
Photosynthetic Apparatus ◽  
Fluorescence Transient ◽  
Chlorophyll A Fluorescence Transient ◽  
Concentration Interval

Abstract When propagated in vitro, explants receive all the nutrients needed for their growth, including carbohydrates, from the culture medium. However, it is not well understood how the type and concentration of carbohydrates can affect the functioning of the photosynthetic apparatus (particularly photosystem II) of these plants. The aim was to assess the morphophysiological responses of Billbergia zebrina plants in function of sources and concentrations of carbohydrates during in vitro culture. Side shoots of plants previously established in vitro were individualized and transferred to a culture medium containing fructose, glucose or sucrose in four concentrations (0, 15, 30 or 45 g L−1). After growth for 55 days, the chlorophyll a fluorescence transient, leaf anatomy and growth were analyzed. The concentration and type of carbohydrate employed during in vitro culture did not decrease the photosynthetic apparatus performance. However, concentrations above 30 g L−1 led to anatomical modifications, revealing some degree of stress suffered by the plants. When grown in concentrations of 15 and 30 g L−1, irrespective of the carbohydrate used, the plants presented greater stomatal density. The supplementation of the culture medium with monosaccharides caused alterations in the development of the xylem vessels, such as increased number and diameter, allowing adjustment to the microenvironmental conditions. The in vitro conditions influenced the photosynthetic and anatomical responses of plants. The concentration interval from 15 to 30 g L−1 sucrose had a better effect by not causing large changes in the performance of the photosynthetic apparatus and anatomy of plants.

A new experimental culture medium for cultivation of Leishmania amazonensis: its efficacy for the continuous in vitro growth and differentiation of infective promastigote forms

2010 ◽  
Vol 106 (5) ◽  
pp. 1249-1252 ◽  
Author(s):  
Igor de Almeida Rodrigues ◽  
Bianca Alcântara da Silva ◽  
André Luis Souza dos Santos ◽  
Alane Beatriz Vermelho ◽  
Celuta Sales Alviano ◽  
...  
Keyword(s):  
Culture Medium ◽  
Leishmania Amazonensis ◽  
In Vitro Growth

scholarly journals In Vitro Growth and Regeneration of Drosera spatulata Labill. on Various Media

HortScience ◽  
1996 ◽  
Vol 31 (6) ◽  
pp. 1033-1034 ◽  
Author(s):  
Mirna Curkovic Perica ◽  
Jasna Berljak
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Sodium Hypochlorite ◽  
Growth Regulators ◽  
Shoot Tips ◽  
Ms Medium ◽  
In Vitro Growth ◽  
In Vitro Multiplication

Conditions for in vitro multiplication and flowering of Drosera spatulata plants were established. Shoot tips of greenhouse-grown plants were sterilized with 1% or 0.5% sodium hypochlorite. The influence of different media concentrations, hormone supplementation, and pH was investigated. Full MS medium without growth regulators was the best for regeneration and multiplication of plants. Regenerated shoots rooted spontaneously on medium without growth regulators and without transfer to additional medium. In 3 months, 100 to 200 plants were generated per explant. Flowering was induced on media supplemented with plant growth regulators. Plants were acclimatized on sterile peat.

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