scholarly journals [RETRACTED ARTICLE]: EVALUATION OF ENEMAS CONTAINING SUCRALFATE IN TISSUE CONTENT OF MUC-2 PROTEIN IN EXPERIMENTAL MODEL OF DIVERSION COLITIS

2018 ◽  
Vol 31 (3) ◽  
Keyword(s):  
Experimental Model ◽  
Diversion Colitis ◽  
Tissue Content ◽  
Retracted Article

scholarly journals [RETRACTED ARTICLE]: EVALUATION OF ENEMAS CONTAINING SUCRALFATE IN TISSUE CONTENT OF MUC-2 PROTEIN IN EXPERIMENTAL MODEL OF DIVERSION COLITIS

2018 ◽  
Vol 31 (3) ◽  
Author(s):  
Oscar Orlando Araya FERNANDEZ ◽  
José Aires PEREIRA ◽  
Fábio Guilherme CAMPOS ◽  
Carolina Mardegan ARAYA ◽  
Gabriele Escocia MARINHO ◽  
...  
Keyword(s):  
Experimental Models ◽  
Histopathological Study ◽  
Diversion Colitis ◽  
Fecal Stream ◽  
Inflammatory Score ◽  
Tissue Content ◽  
Proximal Colostomy ◽  
Computerized Morphometry ◽  
Retracted Article ◽  
Mucous Fistula

ABSTRACT Background: The effects of topical application of sucralfate (SCF) on the tissue content of MUC-2 protein have not yet been evaluated in experimental models of diversion colitis. Aim: To measure the tissue content of MUC-2 protein in the colonic mucosa diverted from fecal stream submitted to the SCF intervention. Methods: Thirty-six rats underwent derivation of intestinal transit through proximal colostomy and distal mucous fistula. The animals were divided into three groups which were submitted application of enemas with saline, SCF 1 g/kg/day and SCF 2 g/kg/day. Each group was divided into two subgroups, according to euthanasia was done after two or four weeks. The colitis diagnosis was established by histopathological study and the inflammatory intensity was evaluated by previously validated scale. The MUC-2 protein was identified by immunohistochemistry and the tissue content was measured computerized morphometry). Results: The application of enemas with SCF in the concentration of 2 g/kg/day reduced inflammatory score of the segments that were diverted from fecal stream. The content of MUC-2 in diverted colon of the animals submitted to the intervention with SCF, independently of intervention period and the used concentration, was significantly greater than animals submitted to the application of enemas containing saline (p< 0.01). The content of MUC-2 after the intervention with SCF in the concentration of 2 g/kg/day was significantly higher when compared to the animals submitted to the application containing SCF at concentration of 1.0 g/kg/day (p<0.01). The tissue content of MUC-2 reached the highest values after intervention with SCF in the concentration of 2 g/kg/day for four weeks (p<0.01). Conclusion: The preventive application of enemas containing SCF reduces the inflammatory score and avoids the reduction of tissue content of MUC-2, suggesting that the substance is a valid therapeutic strategy to preserve the mucus layer that covers the intestinal epithelium.

scholarly journals Evaluation of the application of enemas containing sucralfate in tissue content of neutral and acid mucins in experimental model of diversion colitis

2014 ◽  
Vol 29 (9) ◽  
pp. 544-552 ◽  
Author(s):  
Felipe Mendonça Chaim ◽  
Daniela Tiemi Sato ◽  
Murilo Rocha Rodrigues ◽  
Alice Moreira Dias ◽  
Paulo Pedroso Silveira Júnior ◽  
...  
Keyword(s):  
Experimental Model ◽  
Diversion Colitis ◽  
Tissue Content ◽  
Acid Mucins

W1351 Diversion Colitis: Beneficial Effect of Butyrate and Glutamine Enemas in an Experimental Model

2010 ◽  
Vol 138 (5) ◽  
pp. S-705
Author(s):  
Rodrigo G. Pacheco ◽  
Christiano C. Esposito ◽  
Alberto Schanaider ◽  
Leonardo P. Quintella ◽  
Vera L. Chagas ◽  
...  
Keyword(s):  
Beneficial Effect ◽  
Experimental Model ◽  
Diversion Colitis

Evaluation of the Effects of Biological Therapy with Infliximab in an Experimental Model of Diversion Colitis

2021 ◽  
Author(s):  
Andress Godoy Delben ◽  
Carlos Augusto Real Martinez ◽  
Daniela Tiemi Sato ◽  
José Aires Pereira ◽  
Roberta Lais dos Santos Mendonça ◽  
...  
Keyword(s):  
Experimental Model ◽  
Biological Therapy ◽  
Diversion Colitis

scholarly journals EVALUATION OF ENEMAS CONTAINING SUCRALFATE IN TISSUE CONTENT OF MUC-2 PROTEIN IN EXPERIMENTAL MODEL OF DIVERSION COLITIS

2017 ◽  
Vol 30 (2) ◽  
pp. 132-138 ◽  
Author(s):  
Oscar Orlando Araya FERNANDEZ ◽  
José Aires PEREIRA ◽  
Fábio Guilherme CAMPOS ◽  
Carolina Mardegan ARAYA ◽  
Gabriele Escocia MARINHO ◽  
...  
Keyword(s):  
Colonic Mucosa ◽  
Experimental Models ◽  
Histopathological Study ◽  
Diversion Colitis ◽  
Fecal Stream ◽  
Inflammatory Score ◽  
Tissue Content ◽  
Proximal Colostomy ◽  
Computerized Morphometry ◽  
Mucous Fistula

ABSTRACT Background: The effects of topical application of sucralfate (SCF) on the tissue content of MUC-2 protein have not yet been evaluated in experimental models of diversion colitis. Aim: To measure the tissue content of MUC-2 protein in the colonic mucosa diverted from fecal stream submitted to the SCF intervention. Methods: Thirty-six rats underwent derivation of intestinal transit through proximal colostomy and distal mucous fistula. The animals were divided into three groups which were submitted application of enemas with saline, SCF 1 g/kg/day and SCF 2 g/kg/day. Each group was divided into two subgroups, according to euthanasia was done after two or four weeks. The colitis diagnosis was established by histopathological study and the inflammatory intensity was evaluated by previously validated scale. The MUC-2 protein was identified by immunohistochemistry and the tissue content was measured computerized morphometry). Results: The application of enemas with SCF in the concentration of 2 g/kg/day reduced inflammatory score of the segments that were diverted from fecal stream. The content of MUC-2 in diverted colon of the animals submitted to the intervention with SCF, independently of intervention period and the used concentration, was significantly greater than animals submitted to the application of enemas containing saline (p< 0.01). The content of MUC-2 after the intervention with SCF in the concentration of 2 g/kg/day was significantly higher when compared to the animals submitted to the application containing SCF at concentration of 1.0 g/kg/day (p<0.01). The tissue content of MUC-2 reached the highest values after intervention with SCF in the concentration of 2 g/kg/day for four weeks (p<0.01). Conclusion: The preventive application of enemas containing SCF reduces the inflammatory score and avoids the reduction of tissue content of MUC-2, suggesting that the substance is a valid therapeutic strategy to preserve the mucus layer that covers the intestinal epithelium.

Ultrastructural Alterations in Sinusoidal Endothelial Fenestrae as a Result of Hypoxia

1976 ◽  
Vol 34 ◽  
pp. 168-169
Author(s):  
Waykin Nopanitaya ◽  
Raeford E. Brown ◽  
Joe W. Grisham ◽  
Johnny L. Carson
Keyword(s):  
Endothelial Cells ◽  
Experimental Model ◽  
Hepatic Lobule ◽  
Ultrastructural Alterations ◽  
Large Type ◽  
Sinusoidal Endothelial Fenestrae ◽  
General Size ◽  
Sem Studies

Mammalian endothelial cells lining hepatic sinusoids have been found to be widely fenestrated. Previous SEM studies (1,2) have noted two general size catagories of fenestrations; large fenestrae were distributed randomly while the small type occurred in groups. These investigations also reported that large fenestrae were more numerous and larger in the endothelial cells at the afferent ends of sinusoids or around the portal areas, whereas small fenestrae were more numerous around the centrilobular portion of the hepatic lobule. It has been further suggested that under some physiologic conditions small fenestrae could fuse and subsequently become the large type, but this is, as yet, unproven.We have used a reproducible experimental model of hypoxia to study the ultrastructural alterations in sinusoidal endothelial fenestrations in order to investigate the origin of occurrence of large fenestrae.

174 Early beneficial effects of vasopeptidase inhibtion n an experimental model of heart failure

2003 ◽  
Vol 2 (1) ◽  
pp. 33-34
Author(s):  
B SHIVALKAR ◽  
B MEURIS ◽  
R VANBENEDEN ◽  
J KETESLEGERS ◽  
F BECKERS ◽  
...  
Keyword(s):  
Heart Failure ◽  
Experimental Model ◽  
Beneficial Effects
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