EVALUATION OF ENEMAS CONTAINING SUCRALFATE IN TISSUE CONTENT OF MUC-2 PROTEIN IN EXPERIMENTAL MODEL OF DIVERSION COLITIS

ABSTRACT Background: The effects of topical application of sucralfate (SCF) on the tissue content of MUC-2 protein have not yet been evaluated in experimental models of diversion colitis. Aim: To measure the tissue content of MUC-2 protein in the colonic mucosa diverted from fecal stream submitted to the SCF intervention. Methods: Thirty-six rats underwent derivation of intestinal transit through proximal colostomy and distal mucous fistula. The animals were divided into three groups which were submitted application of enemas with saline, SCF 1 g/kg/day and SCF 2 g/kg/day. Each group was divided into two subgroups, according to euthanasia was done after two or four weeks. The colitis diagnosis was established by histopathological study and the inflammatory intensity was evaluated by previously validated scale. The MUC-2 protein was identified by immunohistochemistry and the tissue content was measured computerized morphometry). Results: The application of enemas with SCF in the concentration of 2 g/kg/day reduced inflammatory score of the segments that were diverted from fecal stream. The content of MUC-2 in diverted colon of the animals submitted to the intervention with SCF, independently of intervention period and the used concentration, was significantly greater than animals submitted to the application of enemas containing saline (p< 0.01). The content of MUC-2 after the intervention with SCF in the concentration of 2 g/kg/day was significantly higher when compared to the animals submitted to the application containing SCF at concentration of 1.0 g/kg/day (p<0.01). The tissue content of MUC-2 reached the highest values after intervention with SCF in the concentration of 2 g/kg/day for four weeks (p<0.01). Conclusion: The preventive application of enemas containing SCF reduces the inflammatory score and avoids the reduction of tissue content of MUC-2, suggesting that the substance is a valid therapeutic strategy to preserve the mucus layer that covers the intestinal epithelium.

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[RETRACTED ARTICLE]: EVALUATION OF ENEMAS CONTAINING SUCRALFATE IN TISSUE CONTENT OF MUC-2 PROTEIN IN EXPERIMENTAL MODEL OF DIVERSION COLITIS

ABCD Arquivos Brasileiros de Cirurgia Digestiva (São Paulo) ◽

10.1590/0102-672020180001e1391 ◽

2018 ◽

Vol 31 (3) ◽

Author(s):

Oscar Orlando Araya FERNANDEZ ◽

José Aires PEREIRA ◽

Fábio Guilherme CAMPOS ◽

Carolina Mardegan ARAYA ◽

Gabriele Escocia MARINHO ◽

...

Keyword(s):

Experimental Models ◽

Histopathological Study ◽

Diversion Colitis ◽

Fecal Stream ◽

Inflammatory Score ◽

Tissue Content ◽

Proximal Colostomy ◽

Computerized Morphometry ◽

Retracted Article ◽

Mucous Fistula

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Influence of the application of enemas with oily extract of curcumin ( Curcuma longa ) on the tissue content of neutral and acidic mucins in the colonic mucosa without fecal stream

Journal of Coloproctology ◽

10.1016/j.jcol.2017.08.002 ◽

2017 ◽

Vol 37 (4) ◽

pp. 312-319

Author(s):

Carlos Augusto Real Martinez ◽

Vitor Piquera de Oliveira ◽

Fabio Guilherme Campos ◽

Danilo Toshio Kanno ◽

Oscar Orlando Araya Fernandez ◽

...

Keyword(s):

Colonic Mucosa ◽

Curcuma Longa ◽

Fecal Stream ◽

Tissue Content

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Claudin-3 and occludin tissue content in the glands of colonic mucosa with and without a fecal stream

Journal of Molecular Histology ◽

10.1007/s10735-015-9610-y ◽

2015 ◽

Vol 46 (2) ◽

pp. 183-194 ◽

Cited By ~ 8

Author(s):

Carlos Augusto Real Martinez ◽

Fabio Guilherme Caserta Maryssael de Campos ◽

Viviel Rodrigo José de Carvalho ◽

Caroline de Castro Ferreira ◽

Murilo Rocha Rodrigues ◽

...

Keyword(s):

Colonic Mucosa ◽

Fecal Stream ◽

Tissue Content

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Tissue content of sulfomucins and sialomucins in the colonic mucosa, without fecal stream, undergoing daily intervention with sucralfate

Acta Cirurgica Brasileira ◽

10.1590/s0102-865020150050000004 ◽

2015 ◽

Vol 30 (5) ◽

pp. 328-338 ◽

Cited By ~ 8

Author(s):

Christiny Emmanuelle Gabriel Bonassa ◽

José Aires Pereira ◽

Fábio Guilherme Caserta Maryssael de Campos ◽

Murilo Rocha Rodrigues ◽

Daniela Tiemi Sato ◽

...

Keyword(s):

Colonic Mucosa ◽

Fecal Stream ◽

Tissue Content

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Reduction in oxidative stress levels in the colonic mucosa without fecal stream after the application of enemas containing aqueous Ilex paraguariensis extract

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502011000400008 ◽

2011 ◽

Vol 26 (4) ◽

pp. 289-296 ◽

Cited By ~ 10

Author(s):

Fernando Lorenzetti da Cunha ◽

Camila Morais Gonçalves da Silva ◽

Marcos Gonçalves de Almeida ◽

Thais Miguel do Monte Lameiro ◽

Letícia Helena Souza Marques ◽

...

Keyword(s):

Oxidative Stress ◽

Aqueous Extract ◽

Tissue Damage ◽

Colonic Mucosa ◽

Ilex Paraguariensis ◽

Histopathological Analysis ◽

Fecal Stream ◽

Proximal Colostomy ◽

Antioxidant Effects ◽

Experimental Group

PURPOSE: To evaluate the antioxidant effects of enemas containing aqueous extract of Ilex paraguariensis, comparing segments with and without fecal stream and correlating the segments with the duration of intervention. METHODS: Twenty-six Wistar rats were subjected to a diversion of the fecal stream in the left colon by a proximal colostomy and distal mucosal fistula. The rats were distributed randomly into two experimental groups of 13 animals each based on the time of sacrifice after surgical procedure (two or four weeks). Each group was then divided into two experimental subgroups that received either second daily enemas containing 0.9% saline solution or aqueous extract of Ilex paraguariensis at 0.2g/100g. Colitis was diagnosed by histopathological analysis and the detection of oxidative tissue damage by measuring the levels of malondialdehyde. The Mann-Whitney test was used to compare the tissue levels of malondialdehyde between colon segments with and without fecal stream in each experimental group, and the Kruskal-Wallis test was used to verify the variance between the levels of oxidative stress according the duration of the irrigation; both tests determined significance at 5% (p<0.05). RESULTS: The levels of malondialdehyde in the animals subjected to intervention in the colon with saline with and without fecal stream after two and four weeks of irrigation were 0.05±0.006 and 0.06±0.006, and 0.05± 0.03 and 0.08 ±0.02, respectively. The malondialdehyde levels in the animals irrigated with Ilex paraguariensis with and without fecal stream after two and four weeks of irrigation were 0.010±0.002 and 0.02±0.004, and 0.03±0.007 and 0.04±0.01, respectively. After two and four weeks of intervention, the levels of malondialdehyde were lower in the animals irrigated with Ilex paraguariensis regardless of the time of irrigation (p=0.0001 and p=0.002, respectively). CONCLUSION: The daily rectal application of enemas containing aqueous extract of Ilex paraguariensis decreases oxidative tissue damage in the colon without fecal stream regardless of the time of irrigation.

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5-Aminosalicylic Acid (5-ASA) Can Reduce Levels of Oxidative DNA Damage in Cells of Colonic Mucosa With and Without Fecal Stream

Digestive Diseases and Sciences ◽

10.1007/s10620-010-1378-z ◽

2010 ◽

Vol 56 (4) ◽

pp. 1037-1046 ◽

Cited By ~ 18

Author(s):

Caroline Caltabiano ◽

Felipe Rodrigues Máximo ◽

Ana Paula Pimentel Spadari ◽

Daniel Duarte da Conceição Miranda ◽

Marcia Milena Pivatto Serra ◽

...

Keyword(s):

Dna Damage ◽

Oxidative Dna Damage ◽

Colonic Mucosa ◽

Aminosalicylic Acid ◽

Fecal Stream ◽

In Cells

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Colonic mucosa in diversion colitis

Human Pathology ◽

10.1016/0046-8177(93)90202-r ◽

1993 ◽

Vol 24 (10) ◽

pp. 1150

Author(s):

Ronald M. Rhatigan

Keyword(s):

Colonic Mucosa ◽

Diversion Colitis

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Enemas with mesalazine increase the tissue contents of mucins in the colonic mucosa devoid of fecal stream

Acta Cirurgica Brasileira ◽

10.1590/s0102-865020190040000006 ◽

2019 ◽

Vol 34 (4) ◽

Author(s):

Carlos Augusto Real Martinez ◽

Fábio Guilherme Campos ◽

Danilo Toshio Kanno ◽

Eli Cristiano Meneses ◽

Gabrielle Maira Matijascic ◽

...

Keyword(s):

Colonic Mucosa ◽

Fecal Stream ◽

Tissue Contents

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Quantification by computerized morphometry of tissue levels of sulfomucins and sialomucins in diversion colitis in rats

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502010000300004 ◽

2010 ◽

Vol 25 (3) ◽

pp. 231-240 ◽

Cited By ~ 20

Author(s):

Carlos Augusto Real Martinez ◽

Ronaldo Nonose ◽

Ana Paula Pimentel Spadari ◽

Felipe Rodrigues Máximo ◽

Denise Gonçalves Priolli ◽

...

Keyword(s):

Tissue Expression ◽

Computer Assisted ◽

Colon Mucosa ◽

Diversion Colitis ◽

Significance Level ◽

Fecal Stream ◽

Post Test ◽

Computer Assisted Image Processing ◽

Computer Assisted Image ◽

Group B

PURPOSE: To quantify the intensity of sulfomucin and sialomucin expression in the colon mucosa, by means of computer-assisted image processing, comparing segments with and without fecal stream and correlating with the duration of fecal transit exclusion. METHODS: Forty-five Wistar rats were subjected to diversion of the fecal stream in the left colon by means of constructing a proximal colostomy and distal mucosal fistula. They were distributed randomly into three experimental groups of 15 animals, of which 10 were subjected to colon diversion (experimental subgroup) and five were only subjected to laparotomy, without colon diversion (control subgroup). The three experimental groups were formed according to the sacrifice date, which was to be performed six weeks after the surgical procedure (Group A), 12 weeks (Group B) and 18 weeks (Group C). The sulfomucin and sialomucin expression in the colon mucosa was evaluated using the histochemical technique of high iron diamine-alcian blue (HID-AB). The tissue expression was quantified for each animal, in the segments with and without fecal stream, at a location where there were four complete contiguous crypts in two random fields, with the aid of the computer-assisted image analysis software. The final value was taken to be the mean reading from the two fields selected, in the segments with and without fecal stream. To compare the expressions of the two mucin subtypes in the segments with and without fecal stream, the paired Student t test was used. To analyze variance according to duration of exclusion, ANOVA with the Newman-Keuls post-test was used, setting the significance level at 5% (p<0.05). RESULTS: There were significant reductions in tissue sulfomucin and sialomucin content in the colon without fecal stream, independent of the duration of exclusion considered. There was increased tissue sulfomucin content and decreased tissue sialomucin in the segments without fecal stream, with increasing duration of exclusion. CONCLUSIONS: Diversion of the fecal transit decreased the tissue sulfomucin and sialomucin content in the segments without fecal stream. Notwithstanding the reduction in the levels of both subtypes of acid mucin in the segments without fecal stream, there was increased tissue sulfomucin content and decreased tissue sialomucin with increasing duration of intestinal diversion.

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