scholarly journals [RETRACTED ARTICLE]: EVALUATION OF ENEMAS CONTAINING SUCRALFATE IN TISSUE CONTENT OF MUC-2 PROTEIN IN EXPERIMENTAL MODEL OF DIVERSION COLITIS

2018 ◽  
Vol 31 (3) ◽  
Author(s):  
Oscar Orlando Araya FERNANDEZ ◽  
José Aires PEREIRA ◽  
Fábio Guilherme CAMPOS ◽  
Carolina Mardegan ARAYA ◽  
Gabriele Escocia MARINHO ◽  
...  
Keyword(s):  
Experimental Models ◽  
Histopathological Study ◽  
Diversion Colitis ◽  
Fecal Stream ◽  
Inflammatory Score ◽  
Tissue Content ◽  
Proximal Colostomy ◽  
Computerized Morphometry ◽  
Retracted Article ◽  
Mucous Fistula

ABSTRACT Background: The effects of topical application of sucralfate (SCF) on the tissue content of MUC-2 protein have not yet been evaluated in experimental models of diversion colitis. Aim: To measure the tissue content of MUC-2 protein in the colonic mucosa diverted from fecal stream submitted to the SCF intervention. Methods: Thirty-six rats underwent derivation of intestinal transit through proximal colostomy and distal mucous fistula. The animals were divided into three groups which were submitted application of enemas with saline, SCF 1 g/kg/day and SCF 2 g/kg/day. Each group was divided into two subgroups, according to euthanasia was done after two or four weeks. The colitis diagnosis was established by histopathological study and the inflammatory intensity was evaluated by previously validated scale. The MUC-2 protein was identified by immunohistochemistry and the tissue content was measured computerized morphometry). Results: The application of enemas with SCF in the concentration of 2 g/kg/day reduced inflammatory score of the segments that were diverted from fecal stream. The content of MUC-2 in diverted colon of the animals submitted to the intervention with SCF, independently of intervention period and the used concentration, was significantly greater than animals submitted to the application of enemas containing saline (p< 0.01). The content of MUC-2 after the intervention with SCF in the concentration of 2 g/kg/day was significantly higher when compared to the animals submitted to the application containing SCF at concentration of 1.0 g/kg/day (p<0.01). The tissue content of MUC-2 reached the highest values after intervention with SCF in the concentration of 2 g/kg/day for four weeks (p<0.01). Conclusion: The preventive application of enemas containing SCF reduces the inflammatory score and avoids the reduction of tissue content of MUC-2, suggesting that the substance is a valid therapeutic strategy to preserve the mucus layer that covers the intestinal epithelium.

scholarly journals EVALUATION OF ENEMAS CONTAINING SUCRALFATE IN TISSUE CONTENT OF MUC-2 PROTEIN IN EXPERIMENTAL MODEL OF DIVERSION COLITIS

2017 ◽  
Vol 30 (2) ◽  
pp. 132-138 ◽  
Author(s):  
Oscar Orlando Araya FERNANDEZ ◽  
José Aires PEREIRA ◽  
Fábio Guilherme CAMPOS ◽  
Carolina Mardegan ARAYA ◽  
Gabriele Escocia MARINHO ◽  
...  
Keyword(s):  
Colonic Mucosa ◽  
Experimental Models ◽  
Histopathological Study ◽  
Diversion Colitis ◽  
Fecal Stream ◽  
Inflammatory Score ◽  
Tissue Content ◽  
Proximal Colostomy ◽  
Computerized Morphometry ◽  
Mucous Fistula

ABSTRACT Background: The effects of topical application of sucralfate (SCF) on the tissue content of MUC-2 protein have not yet been evaluated in experimental models of diversion colitis. Aim: To measure the tissue content of MUC-2 protein in the colonic mucosa diverted from fecal stream submitted to the SCF intervention. Methods: Thirty-six rats underwent derivation of intestinal transit through proximal colostomy and distal mucous fistula. The animals were divided into three groups which were submitted application of enemas with saline, SCF 1 g/kg/day and SCF 2 g/kg/day. Each group was divided into two subgroups, according to euthanasia was done after two or four weeks. The colitis diagnosis was established by histopathological study and the inflammatory intensity was evaluated by previously validated scale. The MUC-2 protein was identified by immunohistochemistry and the tissue content was measured computerized morphometry). Results: The application of enemas with SCF in the concentration of 2 g/kg/day reduced inflammatory score of the segments that were diverted from fecal stream. The content of MUC-2 in diverted colon of the animals submitted to the intervention with SCF, independently of intervention period and the used concentration, was significantly greater than animals submitted to the application of enemas containing saline (p< 0.01). The content of MUC-2 after the intervention with SCF in the concentration of 2 g/kg/day was significantly higher when compared to the animals submitted to the application containing SCF at concentration of 1.0 g/kg/day (p<0.01). The tissue content of MUC-2 reached the highest values after intervention with SCF in the concentration of 2 g/kg/day for four weeks (p<0.01). Conclusion: The preventive application of enemas containing SCF reduces the inflammatory score and avoids the reduction of tissue content of MUC-2, suggesting that the substance is a valid therapeutic strategy to preserve the mucus layer that covers the intestinal epithelium.

scholarly journals Influence of the application of enemas with oily extract of curcumin ( Curcuma longa ) on the tissue content of neutral and acidic mucins in the colonic mucosa without fecal stream

2017 ◽  
Vol 37 (4) ◽  
pp. 312-319
Author(s):  
Carlos Augusto Real Martinez ◽  
Vitor Piquera de Oliveira ◽  
Fabio Guilherme Campos ◽  
Danilo Toshio Kanno ◽  
Oscar Orlando Araya Fernandez ◽  
...  
Keyword(s):  
Colonic Mucosa ◽  
Curcuma Longa ◽  
Fecal Stream ◽  
Tissue Content

scholarly journals Quantification by computerized morphometry of tissue levels of sulfomucins and sialomucins in diversion colitis in rats

2010 ◽  
Vol 25 (3) ◽  
pp. 231-240 ◽  
Author(s):  
Carlos Augusto Real Martinez ◽  
Ronaldo Nonose ◽  
Ana Paula Pimentel Spadari ◽  
Felipe Rodrigues Máximo ◽  
Denise Gonçalves Priolli ◽  
...  
Keyword(s):  
Tissue Expression ◽  
Computer Assisted ◽  
Colon Mucosa ◽  
Diversion Colitis ◽  
Significance Level ◽  
Fecal Stream ◽  
Post Test ◽  
Computer Assisted Image Processing ◽  
Computer Assisted Image ◽  
Group B

PURPOSE: To quantify the intensity of sulfomucin and sialomucin expression in the colon mucosa, by means of computer-assisted image processing, comparing segments with and without fecal stream and correlating with the duration of fecal transit exclusion. METHODS: Forty-five Wistar rats were subjected to diversion of the fecal stream in the left colon by means of constructing a proximal colostomy and distal mucosal fistula. They were distributed randomly into three experimental groups of 15 animals, of which 10 were subjected to colon diversion (experimental subgroup) and five were only subjected to laparotomy, without colon diversion (control subgroup). The three experimental groups were formed according to the sacrifice date, which was to be performed six weeks after the surgical procedure (Group A), 12 weeks (Group B) and 18 weeks (Group C). The sulfomucin and sialomucin expression in the colon mucosa was evaluated using the histochemical technique of high iron diamine-alcian blue (HID-AB). The tissue expression was quantified for each animal, in the segments with and without fecal stream, at a location where there were four complete contiguous crypts in two random fields, with the aid of the computer-assisted image analysis software. The final value was taken to be the mean reading from the two fields selected, in the segments with and without fecal stream. To compare the expressions of the two mucin subtypes in the segments with and without fecal stream, the paired Student t test was used. To analyze variance according to duration of exclusion, ANOVA with the Newman-Keuls post-test was used, setting the significance level at 5% (p<0.05). RESULTS: There were significant reductions in tissue sulfomucin and sialomucin content in the colon without fecal stream, independent of the duration of exclusion considered. There was increased tissue sulfomucin content and decreased tissue sialomucin in the segments without fecal stream, with increasing duration of exclusion. CONCLUSIONS: Diversion of the fecal transit decreased the tissue sulfomucin and sialomucin content in the segments without fecal stream. Notwithstanding the reduction in the levels of both subtypes of acid mucin in the segments without fecal stream, there was increased tissue sulfomucin content and decreased tissue sialomucin with increasing duration of intestinal diversion.

scholarly journals The importance of oxygen free radicals in the etiopathogenesis of diversion colitis in rats

2010 ◽  
Vol 25 (5) ◽  
pp. 387-395 ◽  
Author(s):  
Carlos Augusto Real Martinez ◽  
Marcelo Lima Ribeiro ◽  
Alessandra Gambero ◽  
Daniel Duarte da Conceição Miranda ◽  
José Aires Pereira ◽  
...  
Keyword(s):  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Activity Index ◽  
Neutrophil Infiltration ◽  
Histopathological Analysis ◽  
Diversion Colitis ◽  
Colon Cells ◽  
Significance Level ◽  
Colon Wall ◽  
Fecal Stream

PURPOSE: Quantify the levels of oxidative DNA damage of epithelial colon cells comparing segments with and without fecal stream. METHODS: Sixty Wistar rats were subjected to deviation of fecal stream by proximal colostomy and a distal mucosal fistula. Animals were divided into three experimental groups that were sacrificed 6, 12 and 24 weeks after surgery. In each experimental group, five animals underwent laparotomy without intestinal deviation (sham subgroup). The diagnosis of colitis was made by histopathological analysis and the inflammatory activity index by graduated scale. The neutrophil infiltration was determined by myeloperoxidase tissue levels and the intensity of oxidative DNA damage by comet assay. The Mann-Withney and Student t test were used to compare the results among experimental subgroups and the Kruskal-Wallis test for variance analysis, adopting a significance level of 5% (p<0.05). RESULTS: Colon segments without fecal stream was shown higher histological inflammatory score of the colon wall after 12 and 24 weeks (p=0.001) that increased with the time of diversion (p=0.01). The activity of myeloperoxidase in segments without fecal stream decreased with the time (p=0.001). Oxidative DNA damage levels were significantly higher in the segments without fecal stream, (p=0.0001), independent of time of colon diversion, and increase with the time (p=0.0007). CONCLUSIONS: Colon segments without fecal stream showed high levels of oxidative DNA damage related to histological alterations observed in diversion colitis. The levels of oxidative DNA damage in segments devoid of the fecal stream increase with the time of intestinal exclusion.

scholarly journals Tobacco experimental model to induce urinary bladder neoplasms

2014 ◽  
Vol 41 (1) ◽  
pp. 056-060 ◽  
Author(s):  
José Alexandre Colli Neto ◽  
José Hélio Zen Júnior ◽  
André Del Negro ◽  
Nelson Adami Andreollo ◽  
Marina Rachel Araujo ◽  
...  
Keyword(s):  
Experimental Model ◽  
Bladder Wall ◽  
Bladder Neoplasms ◽  
Urinary Bladder Neoplasms ◽  
Experimental Models ◽  
Histopathological Study ◽  
High Concentrations ◽  
Carbon Dioxide Co2 ◽  
Harmful Effects ◽  
Chronic Use

OBJECTIVE: to develop an experimental model of exposure to tobacco burning (cigarette) products to assess the effects of its chronic use in relation to cancers of the bladder. METHODS: the animals were chronically exposed to the burning tobacco products in a semi-open chamber to simulate smoking. Thirty young Wistar rats were divided into two groups: one with 20 animals simulating smoking for six months, and ten not exposed control animals for the same period. After exposure by inhalation of cigarette smoke, animals were euthanized and subjected to histopathological study of the bladder wall. RESULTS: no tumor was found but mild and non significant alterations. The studies of hemo-oximetry (carboxyhemoglobin and methemoglobin) and the concentration of carbon dioxide (CO2) confirm that the animals were exposed to high concentrations of tobacco smoke and its derivatives. CONCLUSION: no bladder mucosal neoplasia was found in the pathological study of animals. The developed experimental models were highly efficient, practical and easy to use and can be used in other similar studies to determine the harmful effects caused by smoking.

scholarly journals Tissue quantification of neutral and acid mucins in the mucosa of the colon with and without fecal stream in rats

2009 ◽  
Vol 24 (4) ◽  
pp. 267-275 ◽  
Author(s):  
Ronaldo Nonose ◽  
Ana Paula Pimentel Spadari ◽  
Denise Gonçalves Priolli ◽  
Felipe Rodrigues Máximo ◽  
José Aires Pereira ◽  
...  
Keyword(s):  
Periodic Acid ◽  
Tissue Expression ◽  
Computer Assisted ◽  
Periodic Acid Schiff ◽  
Fecal Stream ◽  
Proximal Colostomy ◽  
Post Test ◽  
Male Wistar Rats ◽  
Acid Mucins ◽  
Tissue Quantification

PURPOSE: To quantify the intensity of the expression of neutral and acids mucins in mucosa of the colon with and without fecal stream and to correlate this with the duration of fecal transit diversion. METHODS: Thirty male Wistar rats were subjected to fecal transit deviation in the left colon by a proximal colostomy and a distal mucous fistula. The animals were divided into three experimental groups, according to whether sacrificing would be performed six, 12 or 18 weeks after surgery. The expression of neutral and acid mucins was evaluated using the histochemical techniques of Periodic Acid Schiff and Alcian Blue, respectively. The tissue mucins expression was quantified by computer-assisted image analysis software (NIS-Elements) in the segments with and without fecal stream. Student's paired t test was used to compare the quantities of mucins in colon with or without fecal stream and variance between the experimental groups by ANOVA and Newman-Keuls post-test, establishing level of signification of 5% (p<0.05). RESULTS: There were significant decreased quantities of acid and neutral mucins in the colon without transit, compared with the colon with fecal stream, independent of the duration of exclusion. There was increased expression of neutral mucins in the colon with fecal stream after 12 and 18 weeks of exclusion. There was no increase in the expression of acid mucins in the colon with transit as the duration of fecal transit exclusion progressed. There was increased production of acid mucins in the animals submitted to diversion of the fecal stream for 18 weeks, compared with those subjected to diversion for 6 and 12 weeks. In the colon without fecal stream, there was increased expression of neutral mucins after 12 and 18 weeks of exclusion. CONCLUSIONS: Deviation of the fecal stream decreased the expression of acid and neutral mucins in the segments without fecal transit, compared with segments with transit. Regardless of the reduced expression of acid and neutral mucins in the segments without fecal stream, their tissue expression increased with increasing duration of intestinal deviation.

scholarly journals Claudin-3 and occludin tissue content in the glands of colonic mucosa with and without a fecal stream

2015 ◽  
Vol 46 (2) ◽  
pp. 183-194 ◽  
Author(s):  
Carlos Augusto Real Martinez ◽  
Fabio Guilherme Caserta Maryssael de Campos ◽  
Viviel Rodrigo José de Carvalho ◽  
Caroline de Castro Ferreira ◽  
Murilo Rocha Rodrigues ◽  
...  
Keyword(s):  
Colonic Mucosa ◽  
Fecal Stream ◽  
Tissue Content

scholarly journals Enterotoxic, neurotoxic and cytotoxice demonstrated by shiga toxin (2d) producing escherichia coli in experimental models

2020 ◽  
Vol 46 (1) ◽  
pp. 41-47
Author(s):  
Chowdhury Rafiqul Ahsan ◽  
Kohinur Begum ◽  
Enamul Kabir ◽  
Alam Nur-E- Kamal ◽  
Kaisar Ali Talukder
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Cytotoxic Effect ◽  
Kidney Tissue ◽  
Clinical Manifestations ◽  
Diarrhoeal Disease ◽  
Experimental Models ◽  
Histopathological Study ◽  
Ileal Loop ◽  
Study Results

Background: Shiga toxin (Stx) producing Escherichia coli (STEC) colonize human intestinal tract and their infections have asymptomatic clinical manifestations which cause local and systemic pathological changes. Objectives: This study intended to establish the role of Shiga toxin (Stx2d) in developing clinical manifestations in STEC infections using experimental models. Methods: A total 300 stool samples were screened from hospitalised diarrhoeal patients enrolled in 2% surveillance system at International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b). The stx gene profile including their variants was identified byPCR.stx2d gene positive STEC PT187 was selected for toxin (s) preparation.Toxin was prepared by centrifugation of culture supernatant. Enterotoxicand paralytic-lethal activities were tested in rabbit ileal loops and mice, respectively.  Histopathological study of the rabbit ileal loop segments and different tissues of mice  by paraffin embedded method and stained by H & E staining. Cytotoxic effect was performed on  HeLa cells. Results: Nine STEC strains were identified for stx2 gene positive.  Among them STEC PT187 was found stx2d gene positive strain and selected for toxic activities. Toxin (s) responsible for causing accumulation of fluid in rabbit ileal loops and its segments showed inflammation and enterocytenecrosis. In mouse model, toxin(s) was found to cause hind limbs paralysis and death. Brain, spinal cord and kidney tissue of mice showed histopathological changes. Toxin (s) also showed positive cytotoxic activity in HeLa cell. Conclusion: In this study, results indicated that Stx2d producing E. coli exhibit not only enterotoxic activity, but also cause impaired neurological functions and cytotoxic effect. Bangladesh Med Res Counc Bull 2020; 46(1): 41-47

scholarly journals Tissue content of sulfomucins and sialomucins in the colonic mucosa, without fecal stream, undergoing daily intervention with sucralfate

2015 ◽  
Vol 30 (5) ◽  
pp. 328-338 ◽  
Author(s):  
Christiny Emmanuelle Gabriel Bonassa ◽  
José Aires Pereira ◽  
Fábio Guilherme Caserta Maryssael de Campos ◽  
Murilo Rocha Rodrigues ◽  
Daniela Tiemi Sato ◽  
...  
Keyword(s):  
Colonic Mucosa ◽  
Fecal Stream ◽  
Tissue Content
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