Proteins of the canine seminal plasma

ABSTRACT: Studies have been performed to identify the proteins present in canine seminal plasma (SP) and relate them to sperm quality as well as to discover molecular markers of reproductive tract diseases. There is evidence that heparin-binding proteins, zinc-binding proteins, and lactoferrin as well as the matrix metalloproteinase, superoxide dismutase, catalase, and glutathione peroxidase enzymes are associated with canine sperm quality. Other studies indicate that prolactin and enzymes like arginine esterase, acid phosphatase, and alkaline phosphatase could be successfully used as biomarkers of reproductive disorders. Thus, the present literature review aims to address aspects related to proteins of the canine SP, their influence on fertility, and their importance as biomarkers of reproductive disorders.

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Heparin- and Zn2+-binding proteins from boar seminal plasma.

Acta Biochimica Polonica ◽

10.18388/abp.1999_4116 ◽

1999 ◽

Vol 46 (4) ◽

pp. 935-939 ◽

Cited By ~ 5

Author(s):

D Hołody ◽

J Strzezek

Keyword(s):

Amino Acid ◽

Affinity Chromatography ◽

Molecular Mass ◽

Seminal Plasma ◽

Binding Proteins ◽

Reproductive Tract ◽

Female Reproductive Tract ◽

Heparin Binding ◽

Sds Page ◽

Protein Components

Low molecular mass, heparin-binding proteins from seminal plasma play an important role in gametes interaction whereas plasmatic Zn2+-binding proteins stabilize chromatin and plasmalemma structures and protect spermatozoa in the female reproductive tract. By means of affinity chromatography the heparin- and Zn2+-binding proteins were isolated from boar seminal plasma and both preparations were analyzed by reverse HPLC. Most of the proteins bound to heparine and Zn2+-ions were classified as spermadhesins. Three fractions binding exclusively Zn2+ were isolated. They differ in amino-acid composition, content of glucosamine and content of protein components revealed by SDS/PAGE.

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The antigenic character of zinc-binding proteins and their localization in boar reproductive tract--a preliminary study.

Acta Biochimica Polonica ◽

10.18388/abp.2013_1980 ◽

2013 ◽

Vol 60 (2) ◽

Cited By ~ 1

Author(s):

Marzena Mogielnicka-Brzozowska ◽

Leyland Fraser ◽

Jerzy Strzeżek ◽

Władysław Kordan

Keyword(s):

Seminal Plasma ◽

Indirect Immunofluorescence ◽

Binding Proteins ◽

Reproductive Tract ◽

Protein Complexes ◽

Zinc Binding ◽

Immunofluorescence Technique ◽

Double Immunodiffusion ◽

Antigenic Protein ◽

Indirect Immunofluorescence Technique

In this study immunoelectrophoretic and double immunodiffusion analyses were used to investigate the antigenic character of zinc-binding proteins (ZnBPs), whereas the indirect immunofluorescence technique was used to identify their origin in boar reproductive tract. The mmunoelectrophoretic analysis of ZnBPs of the seminal plasma resulted in the appearance of three antigenic protein complexes, while specific immunoreactivity patterns of the anti-ZnBP serum were detected by double immunodiffusion analysis. Indirect immunofluorescence technique confirmed that ZnBPs were secreted by different reproductive tract tissues, suggesting their contributions to the seminal plasma.

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Heparin-binding proteins of canine seminal plasma

Theriogenology ◽

10.1016/j.theriogenology.2006.02.016 ◽

2006 ◽

Vol 66 (6-7) ◽

pp. 1606-1609 ◽

Cited By ~ 12

Author(s):

Fabiana Ferreira de Souza ◽

Maria Isabel Mello Martins ◽

Carlos Eurico dos Santos Fernandes ◽

Paulo Eduardo Martins Ribolla ◽

Maria Denise Lopes

Keyword(s):

Seminal Plasma ◽

Binding Proteins ◽

Heparin Binding

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Supplementation of Seminal Plasma-Heparin Binding Proteins to Capacitation Medium Increases In Vitro Acrosome Reaction Percentage in Beetal Bucks

Theriogenology Insight - An International Journal of Reproduction in all Animals ◽

10.30954/2277-3371.01.2020.2 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Navjot Singh Dhillon

Keyword(s):

Seminal Plasma ◽

Acrosome Reaction ◽

Binding Proteins ◽

Heparin Binding ◽

Plasma Heparin

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Heparin-Binding Proteins from Seminal Plasma Bind to Bovine Spermatozoa and Modulate Capacitation by Heparin1

Biology of Reproduction ◽

10.1095/biolreprod42.6.899 ◽

1990 ◽

Vol 42 (5-6) ◽

pp. 899-915 ◽

Cited By ~ 119

Author(s):

David J. Miller ◽

Martin A. Winer ◽

Roy L. Ax

Keyword(s):

Seminal Plasma ◽

Binding Proteins ◽

Heparin Binding ◽

Bovine Spermatozoa

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Proteins and Heparin Binding Proteins of Spermatozoa and Seminal Plasma in Beetal Bucks: Purification, Characterization and Role in In vivo Fertility

Indian Journal of Animal Research ◽

10.18805/ijar.b-3974 ◽

2020 ◽

Author(s):

Ranjna S. Cheema ◽

Navjot S. Dhillon ◽

Sumit Singhal

Keyword(s):

Seminal Plasma ◽

Binding Proteins ◽

Proteome Analysis ◽

Fertility Rate ◽

Heparin Binding ◽

Sds Page ◽

Sperm Extract ◽

Sperm Membrane ◽

Special Relevance

Background: The proteome analysis of seminal plasma and spermatozoa is of special relevance in livestock. Heparin binding proteins (HBPs) found in the seminal plasma of several mammals are shown to bind to sperm membrane and affect a series of events that contribute to normal fertility, such as sperm capacitation, formation of the oviduct reservoir and binding to the oocyte. Profiles of HBPs from seminal plasma and sperm membranes have been associated with sperm fertility. Although, HBPs present in the SP are described in several species, but little is known about HBPs in buck. Methods: Seminal plasma (SP) and sperm extracts (SE) of 13 bucks were subjected to heparin-sepharose affinity chromatography. Sperm extract, seminal plasma and purified HBPs and Non-HBPs were fractionated by SDS-PAGE. Total 78 females (6 per buck) were mated with 13 bucks. Bucks were divided into two groups, G-I (high fertile, 83.3-100% FR) and G-II (low fertile, 50-66.7% FR). Relationship between HBPs and fertility rate was observed. Result: SDS – PAGE of SP and SE resulted in resolution of 22 (10-240 kDa) and 21 (10-270 kDa) bands, respectively. Based on fertility rate 15 and 13 kDa proteins were absent in SP of higher number of GI-compared to G-II bucks. Fourteen bands ranging from 10 – 180 kDa and 10 – 150 kDa were separated from SP-NHBP and SP-HBP. SP-HBPs of 75, 35, 30, 28, 25 and 13 kDa were present in higher (28.6%, 42.5%, 26.2%, 40.5%, 14.3% and 36.2%) number of high fertile than low fertile bucks. NHBP and HBP purified from SE resolved into 11 bands ranging from 10 – 135 kDa and 10 – 120 kDa, respectively. SE-HBP of 53 kDa, 50/45 kDa and 25 kDa were present in higher percentage of high fertile than low fertile bucks.

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Heparin Binding Proteins of Black Bengal Buck Semen and Their Correlation with Sperm Characters and Freezability

Indian Journal of Animal Research ◽

10.18805/ijar.b-3849 ◽

2019 ◽

Author(s):

M Karunakaran ◽

Vivek C Gajare ◽

Ajoy Mandal ◽

Mohan Mondal ◽

S K Das ◽

...

Keyword(s):

Seminal Plasma ◽

Binding Proteins ◽

Polyacrylamide Gel Electrophoresis ◽

Sodium Dodecyl ◽

Heparin Binding ◽

Sds Page ◽

Significant Difference ◽

Sperm Characters

This experiment was conducted to study the electrophoretic characters of heparin binding proteins (HBP) of Black Bengal buck semen and their correlation with sperm characters and cryo-survivability. Semen ejaculates (n=20/buck) were collected from nine bucks and in vitro sperm characters were evaluated at collection, after equilibration and after freeze - thawing. HBP were isolated through heparin column and discontinuous Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) was performed to assess molecular weight. Significant difference (plessthan0.01) were observed among the bucks in sperm characters and freezability. Eight protein bands of 17 to 180 kDa in seminal plasma and 7 bands in sperm were found. 180 -136 kDa HBP of seminal plasma and 134-101 kDa HBP of sperm had showed high correlation with in vitro sperm characters. Further studies on identification of these proteins and their correlation with in vivo pregnancy are needed to find their role as marker for buck selection.

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