scholarly journals Influence of Silver Nitrate on Somatic Embryogenesis Induction in Arabica Coffee (Coffea arabica L.)

2019 ◽  
Vol 62 ◽  
Author(s):  
Laura Rojas-Lorz ◽  
Griselda Arrieta-Espinoza ◽  
Marta Valdez-Melara ◽  
Luiz Filipe Protasio Pereira ◽  
Andrés Gatica-Arias
Keyword(s):  
Somatic Embryogenesis ◽  
Silver Nitrate ◽  
Coffea Arabica ◽  
Arabica Coffee ◽  
Coffea Arabica L

scholarly journals The effect of auxin 2,4-D and cytokinin 2-ip on direct somatic embryogenesis formation of Coffea arabica L. leaf explant

2011 ◽  
Vol 27 (2) ◽  
Author(s):  
Rina Arimarsetiowati
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Coffea Arabica ◽  
Callus Formation ◽  
Culture Technique ◽  
Plant Production ◽  
Direct Somatic Embryogenesis ◽  
Arabica Coffee ◽  
Randomized Design ◽  
Coffea Arabica L

One of the propagation technique for coffee plant production is tissue culture. Tissue culture technique for Coffea arabica L. faces some problems, mainly in the planlet formation regenerated from explants. The objective of this experiment was to examine the effect 2,4-D and 2-ip combination on the formation of direct somatic embryogenesis of Coffea arabica L. in leaves explant. Auxin (2,4-D) and cytokinin (2-ip) concentrations of, respectively, 1; 5 µM and 5; 10; 15; 20 were used as treatments. This research was conducted using completely randomized design with 10 replications. Observation to induce somatic embryos was done by quantitatively on number of callus from explant and number of embryogenic callus. Beside that, observation by qualitative descriptive was also done on deve lopment of embryogenesis. The results showed that Arabica coffee leaves explant of AS 2K clones could be induced in all medium combination except 5µM 2,4-D and 20µM 2-ip combination. Arabica coffee leaves explant of S 795, Sigararutang and AS 1 varieties could be induced in all medium combination. The highest frequency of callus formation was found in AS 2K, Sigararutang and AS 1 varieties on medium containing 1µM 2,4-D in combination with 10µM 2-ip, whereas for the S 795 variety on medium containing 5µM 2,4-D in combination with 10µM 2-ip. The highest frequency of embriogenic callus in all Arabica coffee variety could be reached on medium containing 5µM 2,4-D in combination with 15µM 2-ip. Key words : Coffea arabica L., somatic embryogenesis, 2,4-D, 2-ip, tissue culture, leaves, callus embryogenic.

scholarly journals DIRECT AND INDIRECT SOMATIC EMBRYOGENESIS ON ARABICA COFFEE (Coffea arabica)

2013 ◽  
Vol 14 (2) ◽  
pp. 79
Author(s):  
Meynarti Sari Dewi Ibrahim ◽  
Rr. Sri Hartati ◽  
Rubiyo Rubiyo ◽  
Agus Purwito ◽  
Sudarsono Sudarsono
Keyword(s):  
Somatic Embryogenesis ◽  
Coffea Arabica ◽  
Leaf Explants ◽  
Ms Medium ◽  
Regeneration Medium ◽  
Arabica Coffee ◽  
Indirect Somatic Embryogenesis ◽  
Half Strength ◽  
Planting Materials ◽  
Coffea Arabica L

Propagation of Coffea arabica L. through direct and indirect somatic embryogenesis technique is promising for producing large number of coffee seedlings. The objectives of the research were to evaluate methods for direct and indirect somatic embryo-genesis induction of C. arabica var. Kartika. The explants were the youngest fully expanded leaves of arabica coffee. The evalu-ated medium was modified Murashige and Skoog (MS) medium supplemented with a combination of 2.26 µM 2,4-D + 4.54 or 9.08 µM thidiazuron; 4.52 µM 2,4-D + 4.54 or 9.08 µM thidiazuron; or 9.04 µM 2,4-D + 9.08 µM thidiazuron. Both calli (100 mg) and pre-embryos developed from the edge of leaf explants were subcultured into regeneration medium (half strength MS with modified vitamin, supplemented with kinetine 9.30 µM and adenine sulfate 40 mg L-1). The results showed coffee leaf explant cultured on medium containing 2.26 µM 2,4-D + 4.54 or 9.08 µM thidiazuron to induce direct somatic embriogenesis from explant, while that of 4.52 or 9.04 µM 2,4-D + 9.08 µM thidiazuron to induced indirect somatic embrio-genesis. The medium for calli induction from coffee by explants was medium supplemented with 4.52 or 9.04 µM 2,4-D in combination with 9.08 µM thidiazuron. On the other hand, the best medium for activation of induction of somatic embryos was MS medium supplemented with 9.04 µM 2,4-D + 9.08 µM thidiazuron. Based on this results, the first step for developing micropropagation for coffee has been resolved. The subsequent studies will be directed to evaluate agronomic performance of the derived planting materials.

scholarly journals DIRECT AND INDIRECT SOMATIC EMBRYOGENESIS ON ARABICA COFFEE (Coffea arabica)

2013 ◽  
Vol 14 (2) ◽  
pp. 79 ◽  
Author(s):  
Meynarti Sari Dewi Ibrahim ◽  
Rr. Sri Hartati ◽  
Rubiyo Rubiyo ◽  
Agus Purwito ◽  
Sudarsono Sudarsono
Keyword(s):  
Somatic Embryogenesis ◽  
Coffea Arabica ◽  
Leaf Explants ◽  
Ms Medium ◽  
Regeneration Medium ◽  
Arabica Coffee ◽  
Indirect Somatic Embryogenesis ◽  
Half Strength ◽  
Planting Materials ◽  
Coffea Arabica L

Propagation of Coffea arabica L. through direct and indirect somatic embryogenesis technique is promising for producing large number of coffee seedlings. The objectives of the research were to evaluate methods for direct and indirect somatic embryo-genesis induction of C. arabica var. Kartika. The explants were the youngest fully expanded leaves of arabica coffee. The evalu-ated medium was modified Murashige and Skoog (MS) medium supplemented with a combination of 2.26 µM 2,4-D + 4.54 or 9.08 µM thidiazuron; 4.52 µM 2,4-D + 4.54 or 9.08 µM thidiazuron; or 9.04 µM 2,4-D + 9.08 µM thidiazuron. Both calli (100 mg) and pre-embryos developed from the edge of leaf explants were subcultured into regeneration medium (half strength MS with modified vitamin, supplemented with kinetine 9.30 µM and adenine sulfate 40 mg L-1). The results showed coffee leaf explant cultured on medium containing 2.26 µM 2,4-D + 4.54 or 9.08 µM thidiazuron to induce direct somatic embriogenesis from explant, while that of 4.52 or 9.04 µM 2,4-D + 9.08 µM thidiazuron to induced indirect somatic embrio-genesis. The medium for calli induction from coffee by explants was medium supplemented with 4.52 or 9.04 µM 2,4-D in combination with 9.08 µM thidiazuron. On the other hand, the best medium for activation of induction of somatic embryos was MS medium supplemented with 9.04 µM 2,4-D + 9.08 µM thidiazuron. Based on this results, the first step for developing micropropagation for coffee has been resolved. The subsequent studies will be directed to evaluate agronomic performance of the derived planting materials.

Teratogenic Effects of Arabica Coffee (Coffea arabica L) on Rats During the Organogenesis Period

2021 ◽  
Vol 3 (2) ◽  
pp. 25-31
Author(s):  
Reza Destri Anggi ◽  
Yuandani ◽  
Aminah Dalimunthe
Keyword(s):  
Weight Loss ◽  
Body Length ◽  
Coffea Arabica ◽  
Teratogenic Effect ◽  
Teratogenic Effects ◽  
Arabica Coffee ◽  
Coffea Arabica L

The purpose of the research was to evaluate the teratogenic effect caused by theprovision of Arabica coffee solution with variations of 0.36 ml, 0.72 ml and 1.08 ml withpositive control of caffeine at a dose of 300 mg / kg bw to rats during organogenesis. Themeasurement parameters in this study are the appearance of reproduction, externalmalformations and scalal malformations. The results in this study found that theadministration of Arabica coffee solution with a volume of 1.08 ml and caffeine 300 mg /kg bb caused an abnormality in the reproductive appearance of weight loss and body length,whereas for extrenal malformation and scalal malforation no abnormalities were found ineach administration of the solution Arabica coffee and caffeine.  

scholarly journals Formulation and evaluation of body splash from Java preanger Arabica coffee (Coffea arabica L.) oil

2019 ◽  
Vol 1402 ◽  
pp. 055092
Author(s):  
R Handayani ◽  
N Auliasari ◽  
T K Oktaviany ◽  
S Hindun ◽  
F F Sriarumtias
Keyword(s):  
Coffea Arabica ◽  
Arabica Coffee ◽  
Coffea Arabica L

Ethylene-induced fruit ripening in arabica coffee (Coffea arabica L.)

1992 ◽  
Vol 32 (3) ◽  
pp. 401 ◽  
Author(s):  
EC Winston ◽  
M Hoult ◽  
CJ Howitt ◽  
RK Shepherd
Keyword(s):  
Coffea Arabica ◽  
Leaf Abscission ◽  
Maturity Stage ◽  
Early Application ◽  
Arabica Coffee ◽  
Fruit Abscission ◽  
Time Of Application ◽  
Berry Weight ◽  
Berry Ripening ◽  
Coffea Arabica L

The effects of ethephon on arabica coffee (Coffea arabica L.) were tested in experiments conducted over 2 years in North Queensland. Rates tested were 0, 125, 250, 500, 750, 1000, 1500, and 2000 mgkg, applied when 15-20% (early treatment) or 45-50% (late treatment) of fruit were coloured. Measurements were made on the degree of leaf and fruit abscission, berry ripening, and berry weight. Ethephon did not cause significant fruit abscission, but rates >500 mg/kg caused unacceptable leaf abscission. Rates of 250 mg/kg were marginally acceptable in terms of leaf abscission. Excessive defoliation led to dieback, which resulted in a reduced crop the following season. Ethephon concentrated berry ripening, significantly increased the number of red (mature) berries, and decreased the number of green (immature) berries. Rates of >500 mg/kg were effective in accelerating berry ripening, while 125 mg/kg had a limited effect. Maturity stage of the bean at time of application helped to determine the ethephon response; early application of ethephon had a more pronounced effect on berry ripening than late application. Ethephon applied early at rates of 1500 and 2000 mg/kg decreased fresh berry weight compared with all other treatments but parchment weight was unaffected. Selection of a suitable rate of ethephon from these data is difficult because berry ripening is offset by leaf drop. Variability of response was also observed.

scholarly journals Biochemical compounds of Arabica coffee (Coffea arabica L.) varieties grown in northwestern highlands of Ethiopia

2020 ◽  
Vol 6 (1) ◽  
pp. 1741319
Author(s):  
Melese Wale Mengistu ◽  
Melkamu Alemayehu Workie ◽  
Abrar Sualeh Mohammed ◽  
Fatih Yildiz
Keyword(s):  
Coffea Arabica ◽  
Arabica Coffee ◽  
Biochemical Compounds ◽  
Coffea Arabica L
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