Teratogenic Effects of Arabica Coffee (Coffea arabica L) on Rats During the Organogenesis Period

2021 ◽  
Vol 3 (2) ◽  
pp. 25-31
Author(s):  
Reza Destri Anggi ◽  
Yuandani ◽  
Aminah Dalimunthe

The purpose of the research was to evaluate the teratogenic effect caused by theprovision of Arabica coffee solution with variations of 0.36 ml, 0.72 ml and 1.08 ml withpositive control of caffeine at a dose of 300 mg / kg bw to rats during organogenesis. Themeasurement parameters in this study are the appearance of reproduction, externalmalformations and scalal malformations. The results in this study found that theadministration of Arabica coffee solution with a volume of 1.08 ml and caffeine 300 mg /kg bb caused an abnormality in the reproductive appearance of weight loss and body length,whereas for extrenal malformation and scalal malforation no abnormalities were found ineach administration of the solution Arabica coffee and caffeine.  

Author(s):  
Rina Arimarsetiowati

One of the propagation technique for coffee plant production is tissue culture. Tissue culture technique for Coffea arabica L. faces some problems, mainly in the planlet formation regenerated from explants. The objective of this experiment was to examine the effect 2,4-D and 2-ip combination on the formation of direct somatic embryogenesis of Coffea arabica L. in leaves explant. Auxin (2,4-D) and cytokinin (2-ip) concentrations of, respectively, 1; 5 µM and 5; 10; 15; 20 were used as treatments. This research was conducted using completely randomized design with 10 replications. Observation to induce somatic embryos was done by quantitatively on number of callus from explant and number of embryogenic callus. Beside that, observation by qualitative descriptive was also done on deve lopment of embryogenesis. The results showed that Arabica coffee leaves explant of AS 2K clones could be induced in all medium combination except 5µM 2,4-D and 20µM 2-ip combination. Arabica coffee leaves explant of S 795, Sigararutang and AS 1 varieties could be induced in all medium combination. The highest frequency of callus formation was found in AS 2K, Sigararutang and AS 1 varieties on medium containing 1µM 2,4-D in combination with 10µM 2-ip, whereas for the S 795 variety on medium containing 5µM 2,4-D in combination with 10µM 2-ip. The highest frequency of embriogenic callus in all Arabica coffee variety could be reached on medium containing 5µM 2,4-D in combination with 15µM 2-ip. Key words : Coffea arabica L., somatic embryogenesis, 2,4-D, 2-ip, tissue culture, leaves, callus embryogenic.


2019 ◽  
Vol 1402 ◽  
pp. 055092
Author(s):  
R Handayani ◽  
N Auliasari ◽  
T K Oktaviany ◽  
S Hindun ◽  
F F Sriarumtias

1992 ◽  
Vol 32 (3) ◽  
pp. 401 ◽  
Author(s):  
EC Winston ◽  
M Hoult ◽  
CJ Howitt ◽  
RK Shepherd

The effects of ethephon on arabica coffee (Coffea arabica L.) were tested in experiments conducted over 2 years in North Queensland. Rates tested were 0, 125, 250, 500, 750, 1000, 1500, and 2000 mgkg, applied when 15-20% (early treatment) or 45-50% (late treatment) of fruit were coloured. Measurements were made on the degree of leaf and fruit abscission, berry ripening, and berry weight. Ethephon did not cause significant fruit abscission, but rates >500 mg/kg caused unacceptable leaf abscission. Rates of 250 mg/kg were marginally acceptable in terms of leaf abscission. Excessive defoliation led to dieback, which resulted in a reduced crop the following season. Ethephon concentrated berry ripening, significantly increased the number of red (mature) berries, and decreased the number of green (immature) berries. Rates of >500 mg/kg were effective in accelerating berry ripening, while 125 mg/kg had a limited effect. Maturity stage of the bean at time of application helped to determine the ethephon response; early application of ethephon had a more pronounced effect on berry ripening than late application. Ethephon applied early at rates of 1500 and 2000 mg/kg decreased fresh berry weight compared with all other treatments but parchment weight was unaffected. Selection of a suitable rate of ethephon from these data is difficult because berry ripening is offset by leaf drop. Variability of response was also observed.


2020 ◽  
Vol 6 (1) ◽  
pp. 1741319
Author(s):  
Melese Wale Mengistu ◽  
Melkamu Alemayehu Workie ◽  
Abrar Sualeh Mohammed ◽  
Fatih Yildiz

2020 ◽  
Vol 31 (2) ◽  
pp. 75
Author(s):  
Desy Muliana Wenas ◽  
Lisana Sidqi Aliya ◽  
Nita Usikatul Janah

<em>The coffee leaf contains flavonoid compounds that are known as an antiinflammation agent. The study aimed to determine antiinflammatory activity of arabica coffee's leaf extract (</em>Coffea arabica<em> L.) against edema on white rat soles of carrageenan-induced rats. The research began with the maceration extraction of coffee leaf in 70% ethanol. The inflammation test was held by measuring the formation of edema using a plethysmometer. The dosages of arabica coffee leaf extract used were 60 mg.kg<sup>-1</sup>, 120 mg.kg<sup>-1</sup>, and 180 mg.kg<sup>-1</sup> rat weight of white Sprague Dawley rat (</em>Rattus norvegicus<em>). The data were analyzed by Kruskal Wallis and Mann Whitney non-parametric tests. Phytochemical screening showed that arabica coffee leaf extract contained flavonoid, saponin, alkaloid, tannin and triterpenoids compound. The arabica coffee leaf extract showed the best antiinflammatory activity at the dose of 180 mg.kg<sup>-1</sup> rat weight with 71.66% inhibition percentage at the 5<sup>th</sup> hour, which was better than the positive control (66.91% edema inhibition at the same hour). This result determined that the leaf extract of arabica coffee had antiinflammatory activity. However, it is necessary to study the acute and chronic toxicity and formulates the arabica coffee leaf extract as a pharmacy product.</em>


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