scholarly journals Prevalence of Dysmicoccus brevipes (Cockerell, 1893) (Hemiptera: Pseudococcidae) in MD2 pineapple crop areas in Colombia

2021 ◽  
Vol 51 ◽  
Author(s):  
Isabel Moreno ◽  
Rubilma Tarazona-Velásquez ◽  
Yenifer Campos-Patiño ◽  
Kevin Alejandro Rodríguez-Arévalo ◽  
Takumasa Kondo
Keyword(s):  
Growth Stages ◽  
Dysmicoccus Brevipes ◽  
Mealybug Wilt ◽  
Valle Del Cauca ◽  
Pineapple Mealybug Wilt ◽  
Pineapple Mealybug ◽  
Agroclimatic Zones

ABSTRACT Pineapple mealybug wilt has been reported as one of the most important diseases affecting pineapple crops worldwide. This study aimed to determine the prevalence of mealybugs associated with two agroclimatic zones of pineapple production in the department of Valle del Cauca, Colombia. The survey was conducted in the upper (Dagua, Restrepo, La Cumbre and Vijes) and lower (Buga and Palmira) land zones. A total of 75 MD2 pineapple plots in different development growth stages were evaluated. The mealybugs were recorded mainly in the upper land zone, with a prevalence of 32 %. Concerning the plots growth stages, the highest prevalence was observed in second-cycle plots (13.3 %), followed by third-cycle ones, including abandoned plots (9.3 %), and first-cycle plots (6.7 %), which were associated with the use of pesticides. Dysmicoccus brevipes was the only mealybug species (Pseudococcidae) found on pineapples in the study area.

scholarly journals Probing behavior of Dysmicoccus brevipes mealybug in pineapple plants1

2016 ◽  
Vol 46 (4) ◽  
pp. 458-463
Author(s):  
Lenira Viana Costa Santa-Cecília ◽  
◽  
Ernesto Prado ◽  
Brígida Souza ◽  
Keyword(s):  
Insect Vector ◽  
Recording Time ◽  
Defense Strategies ◽  
Phenolic Contents ◽  
Probing Behavior ◽  
Epg Technique ◽  
Dysmicoccus Brevipes ◽  
Mealybug Wilt ◽  
Pineapple Mealybug Wilt ◽  
Pineapple Mealybug

ABSTRACT Differences in susceptibility to viruses in plants may be partially explained by the insect vector probing behavior and by the presence of phenolic compounds, which are often associated with defense strategies. This study aimed at detecting barriers that may difficult the probing activity of the Dysmicoccus brevipes (Pseudococcidae) pineapple mealybug, a vector of the pineapple mealybug wilt-associated virus, as well as evaluating the phenolic content of plants, in order to verify any possible relationship with the probing behavior, by using the electrical penetration graphs (EPG) technique. Seedlings of 'Smooth Cayenne' and 'Pérola' pineapple cultivars were used in the experiments. Only 28 % and 21 % of the mealybugs reached the phloem of the 'Smooth Cayenne' and 'Pérola' cultivars, respectively, over 16 h of recording, with an average of 9 h to reach the phloem vessels. The xylem phase was extended in both cultivars and represented approximately 31 % ('Smooth Cayenne') and 44 % ('Pérola') of the recording time. The phenolic contents of both cultivars were similar.

scholarly journals Transmission of Pineapple Mealybug Wilt-Associated Virus by Two Species of Mealybug (Dysmicoccus spp.)

1998 ◽  
Vol 88 (11) ◽  
pp. 1224-1230 ◽  
Author(s):  
D. M. Sether ◽  
D. E. Ullman ◽  
J. S. Hu
Keyword(s):  
Older Adult ◽  
Virus Spread ◽  
Adult Females ◽  
Detached Leaves ◽  
Tissue Blot Immunoassay ◽  
Dysmicoccus Brevipes ◽  
Mealybug Wilt ◽  
Pineapple Mealybug Wilt ◽  
Pineapple Mealybug ◽  
Vector Efficiency

Closterovirus-like particles associated with mealybug wilt of pineapple were acquired and transmitted by the pink pineapple mealybug, Dysmicoccus brevipes, and the gray pineapple mealybug, D. neobrevipes. Mealybugs acquired pineapple mealybug wilt-associated virus (PMWaV) from infected pineapple plants or detached leaves. The virus was detected in plants by tissue blot immunoassay and confirmed by immunosorbent electron microscopy. Plants exposed to mealybugs reared on PMWaV-free pineapple tissue remained uninfected. The presence of ants was correlated with an increased rate of virus spread when caged with D. brevipes. All stages of D. neobrevipes acquired PMWaV, although vector efficiency decreased significantly in older adult females. The probability of a single third-instar immature transmitting the virus was 0.04. Both species of mealybug acquired and transmitted PMWaV from infected pineapple material that had been clonally propagated for decades, and both species acquired PMWaV from sources previously infected with the virus by the other mealybug species.

scholarly journals Spatial and Temporal Incidences of Pineapple mealybug wilt-associated viruses in Pineapple Planting Blocks

Plant Disease ◽  
2010 ◽  
Vol 94 (2) ◽  
pp. 196-200 ◽  
Author(s):  
D. M. Sether ◽  
W. B. Borth ◽  
M. J. Melzer ◽  
J. Hu
Keyword(s):  
Ananas Comosus ◽  
Ratoon Crop ◽  
Virus Incidence ◽  
Central Regions ◽  
Mealybug Wilt ◽  
Pineapple Mealybug Wilt ◽  
Pineapple Mealybug ◽  
Fruit Harvest ◽  
Propagation Material

A complex of Pineapple mealybug wilt-associated viruses (PMWaVs) that can infect pineapple (Ananas comosus) is correlated with reduced yields and mealybug wilt of pineapple. The incidences of PMWaV-1 and PMWaV-2 at planting, fruit harvest, the beginning of the ratoon crop, and ratoon fruit harvest were determined for end, side, and central regions of planting blocks in eight commercial fields. Differences in virus incidence for the three regions at ratoon harvest were highly significant (P = 0.0018). Central regions of planting blocks had lower virus incidences at the time of ratoon fruit harvest. Collection of propagation material from the central regions of planting blocks will help to minimize PMWaV incidence in fields planted with this material.

PINEAPPLE MEALYBUG WILT ASSOCIATED VIRUS AND MEALYBUG WILT OF PINEAPPLE

2005 ◽  
pp. 209-212 ◽  
Author(s):  
J.S. Hu ◽  
D.M. Sether ◽  
M.J. Metzer ◽  
E. Pérez ◽  
A. Gonsalves ◽  
...  
Keyword(s):  
Mealybug Wilt ◽  
Pineapple Mealybug Wilt ◽  
Pineapple Mealybug

Further genomic characterization of pineapple mealybug wilt-associated viruses using high-throughput sequencing

2020 ◽  
Vol 45 (1) ◽  
pp. 64-72 ◽  
Author(s):  
James C. Green ◽  
Maher A. Rwahnih ◽  
Alejandro Olmedo-Velarde ◽  
Michael J. Melzer ◽  
Islam Hamim ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Genomic Characterization ◽  
Mealybug Wilt ◽  
Pineapple Mealybug Wilt ◽  
Pineapple Mealybug

scholarly journals Molecular diagnosis of Fusarium guttiforme and Pineapple mealybug wilt-associated virus

2014 ◽  
Vol 8 (S4) ◽  
Author(s):  
Lorena Carnielli ◽  
Walkíria Andrade Amorim ◽  
Aline Vaz ◽  
Patricia Machado Bueno Fernandes ◽  
José Aires Ventura
Keyword(s):  
Molecular Diagnosis ◽  
Mealybug Wilt ◽  
Pineapple Mealybug Wilt ◽  
Pineapple Mealybug

CHARACTERIZATION AND CONTROL OF PINEAPPLE MEALYBUG WILT ASSOCIATED AMPELOVIRUSES

2006 ◽  
pp. 23-27 ◽  
Author(s):  
E.P. Perez ◽  
D.M. Sether ◽  
M.J. Melzer ◽  
J.L. Busto ◽  
C. Nagai ◽  
...  
Keyword(s):  
Mealybug Wilt ◽  
Pineapple Mealybug Wilt ◽  
Pineapple Mealybug ◽  
And Control

scholarly journals Differentiation, Distribution, and Elimination of Two Different Pineapple mealybug wilt-associated viruses Found in Pineapple

Plant Disease ◽  
2001 ◽  
Vol 85 (8) ◽  
pp. 856-864 ◽  
Author(s):  
D. M. Sether ◽  
A. V. Karasev ◽  
C. Okumura ◽  
C. Arakawa ◽  
F. Zee ◽  
...  
Keyword(s):  
Agricultural Research ◽  
The United States ◽  
United States Department ◽  
Rt Pcr ◽  
Apical Bud ◽  
The World ◽  
Pcr Assays ◽  
Mealybug Wilt ◽  
Pineapple Mealybug Wilt ◽  
Pineapple Mealybug

Surveys for Pineapple mealybug wilt-associated virus-1 (PMWaV-1) and PMWaV-2 were conducted on pineapple samples from Hawaii and around the world. Tissue blot immunoassays (TBIAs) with two different monoclonal antibodies (MAb) specific to either PMWaV-1 or PMWaV-2 indicated that both closteroviruses are widely distributed throughout the pineapple-growing areas of the world. In the worldwide survey, PMWaV-1 was found in 80% of the mea-lybug wilt of pineapple (MWP)-symptomatic and 78% of the asymptomatic pineapple plants tested. A subset of plants was tested for PMWaV-2; 100% of the symptomatic plants and 12% of the asymptomatic plants were positive for this virus. A reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to differentiate between PMWaV-1 and PMWaV-2. Oligonucleotide primers were designed using distinct regions of the HSP 70 homolog genes of the two viruses. PMWaV-specific RT-PCR assays and TBIAs were used to screen the pineapple accessions maintained at the United States Department of Agriculture-Agricultural Research Service National Clonal Germplasm Repository for PMWaV infection; 73% of the accessions were found infected with at least one PMWaV. Pineapple accessions found PMWaV-free were challenged with viruliferous mealybugs to test for immunity to PMWaV-1. No immune germ plasm was identified. Potential alternative virus hosts were screened for infection with virus-specific RT-PCR assays and TBIAs and were also challenged with viruliferous mealybugs. No alternate hosts of PMWaV-1 or PMWaV-2 were identified. PMWaV-1 infection was eliminated through axillary and apical bud propagation from infected crowns. Strategies to manage MWP are discussed.

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