In vitro shoot regeneration in Myracrodruon urundeuva Fr. All

2021 ◽  
Vol 51 ◽  
Author(s):  
Tecla dos Santos Silva ◽  
Rosembrando Sosthenes Leite Carvalho Filho ◽  
Priscila Tavares Fonseca ◽  
José Raniere Ferreira de Santana
Keyword(s):  
Shoot Regeneration ◽  
Callus Formation ◽  
Cotyledonary Node ◽  
Quadratic Equation ◽  
Naphthaleneacetic Acid ◽  
Nodal Segment ◽  
The Mean ◽  
In Vitro Shoot Regeneration ◽  
Number Of Shoots

ABSTRACT Myracrodruon urundeuva Fr. All. is a tree threatened with extinction, which has wood and medicinal potential. This study aimed to analyze the in vitro shoot regeneration in M. urundeuva, in order to increase the species multiplication. Two experiments were conducted: 1) concentrations of 6-benzylaminopurine (BAP) (0.0, 2.0, 4.0, 8.0 and 16.0 µM), in association with naphthaleneacetic acid (NAA) (0.0, 1.5 and 3.0 µM), in explants (cotyledon, hypocotyl and cotyledonary node); 2) concentrations of meta-topolin (mT) (0.0, 2.0, 4.0, 8.0, 16.0 and 32.0 µM) in explants (biaxillary, medial uniaxillary and apical basal nodal segment). The percentage of explants responsive to shoot regeneration, percentage of callus explants, number of shoots and shoot length were evaluated. In the first experiment, the shoot regeneration occurred only in explants of the cotyledonary node and hypocotyl type, with the highest responsiveness percentage (76.67 %) and number of shoots (1.97 and 1.63) obtained for the cotyledonary node in the presence of 3.0 µM of NAA in association with 2.0 (1.97 shoots/explant) and 4.0 µM (1.63 shoots/explant) of mT. In the second experiment, the resolution of the obtained quadratic equation indicates that the use of basal explant with 24.59 µM of mT added to the culture medium leads to the highest number of shoots (1.86). However, despite the mT having increased the mean number of shoots, all treatments containing this cytokinin showed callus formation. As a conclusion, it is possible to regenerate shoots in M. urundeuva from the cotyledonary node using BAP in association with NAA.

scholarly journals In vitro shoot regeneration of boldo from leaf explants

2010 ◽  
Vol 40 (10) ◽  
pp. 2210-2213
Author(s):  
Monalize Salete Mota ◽  
Juliana de Magalhães Bandeira ◽  
Eugenia Jacira Bolacel Braga ◽  
Valmor João Bianchi ◽  
José Antonio Peters
Keyword(s):  
Shoot Regeneration ◽  
Leaf Explants ◽  
Shoot Development ◽  
High Potential ◽  
Naphthaleneacetic Acid ◽  
Regeneration System ◽  
Bud Induction ◽  
Molecular Studies ◽  
In Vitro Shoot Regeneration

A shoot regeneration system for Plectranthus neochilus was studied from leaf explants. Leaves developed under in vitro conditions were cultured on Wood Plant Medium supplemented with 0.2mg dm-3 α-naphthaleneacetic acid (NAA) and different 6-benzilaminopurine (BAP) or thidiazuron (TDZ) concentrations (0, 1.5, 3.0, 4.5 and 6.0mg dm-3). An increase in percentage of responsive explants (85.3%) and in the number of shoots developed per explant (3.2) was observed when the explants were treated with 5.3 and 4.7mg dm-3 BAP, respectively. The leaf explants cultured on media supplemented with TDZ became vitreous and did not form buds. The regeneration system used is efficient for boldo bud induction and shoot development, showing high potential for advanced cellular and molecular studies.

scholarly journals Adventitious Shoot Regeneration and Rooting of Prunus serotina In Vitro Cultures

HortScience ◽  
2006 ◽  
Vol 41 (1) ◽  
pp. 193-201 ◽  
Author(s):  
Ana Carolina Espinosa ◽  
Paula M. Pijut ◽  
Charles H. Michler
Keyword(s):  
Shoot Regeneration ◽  
Adventitious Shoots ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Prunus Serotina ◽  
Naphthaleneacetic Acid ◽  
Eastern United States ◽  
Continuous Darkness ◽  
Number Of Shoots

A complete regeneration protocol was developed for Prunus serotina Ehrh., an important hardwood species for timber and sawlog production in the central and eastern United States. Nodal sections were cultured on Murashige and Skoog (MS) medium supplemented with 4.44 μm 6-benzylaminopurine (BA), 0.49 μm indole-3-butyric acid (IBA), and 0.29 μm gibberellic acid (GA3). In vitro leaf explants of three genotypes were placed on woody plant medium (WPM) supplemented with 0, 2.27, 4.54, or 6.81 μm thidiazuron (TDZ) in combination with 0, 0.54, 1.07, or 5.37 μm naphthaleneacetic acid (NAA), and on WPM supplemented with 0, 4.44, 8.88, or 13.32 μm BA in combination with 0, 0.54, 1.07, or 5.37 μm NAA. Cultures were maintained either in continuous darkness for 5 weeks, or in the dark for 3 weeks and then transferred to a 16-hour photoperiod. TDZ and the genotype had a significant effect on the number of shoots regenerated. The maximum mean number of shoots regenerated per explant (5.05 ± 1.14) was obtained with 2.27 μm TDZ plus 0.54 μm NAA with the 3-week dark period then light treatment. The highest percent shoot regeneration (38.3) and mean number of shoots (4.13 ± 0.97) was obtained with 6.81 μm TDZ plus 1.07 μm NAA. The highest rooting (27%) of adventitious shoots and number of roots per shoot (2.3 ± 0.2) was obtained with 2.5 μm IBA when shoots were maintained for 7 days in the dark on rooting medium before transfer to a 16-hour photoperiod. The highest rooting (70%) of nodal explant-derived stock cultures and number of roots per shoot (2.7 ± 0.9) was also obtained with 2.5 μm IBA, but when shoots were maintained for 4 days in the dark before transfer to a 16-hour photoperiod. In total, 86% of the plantlets survived acclimatization to the greenhouse and 100% survival after overwintering in cold-storage.

scholarly journals 108 Shoot Regeneration from Ovaries of Various Cultivars of Hosta

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 460B-460
Author(s):  
David J. Williams ◽  
Karim H. Al-Juboory
Keyword(s):  
Shoot Regeneration ◽  
Adventitious Shoots ◽  
Shoot Tips ◽  
Effective Alternative ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Slow Process ◽  
Number Of Shoots

The objective of this study was to evaluate the ability of various cultivars of Hosta ovary explants to generate adventitious shoots and obtain variegated plants in vitro. Immature inflorescences along with 8 to 10 cm of scape were harvested from Hosta cultivars. The ovaries were prepared for culture by cutting immature florets before anthesis. The florets were first cut just above the top of the immature ovary to remove the sigma, style, corolla, and anther. Then the calyx and filament bases were also removed. Ovaries were transversely cut into halves and transferred to baby jars containing Hosta initiation medium supplemented with naphthaleneacetic acid (NAA) at 0.5 mg/L and 6-benzylamino purine (BA). The explants produced adventitious shoots from ovary base via organogenesis. The number of shoots regenerated from shoot tips and callus increased linearly with repeatedf subculturing on MS medium. This method would provide an effective alternative to conventional propagation crown division of Hosta, an expensive and slow process. The long-term goal of this project is to improve Hosta.

scholarly journals In Vitro Shoot Regeneration and Polyploid Induction of Rhododendron ‘Fragrantissimum Improved’

HortScience ◽  
2010 ◽  
Vol 45 (5) ◽  
pp. 801-804 ◽  
Author(s):  
Cary J. Hebert ◽  
Darren H. Touchell ◽  
Thomas G. Ranney ◽  
Anthony V. LeBude
Keyword(s):  
Shoot Regeneration ◽  
In Vitro Regeneration ◽  
Naphthaleneacetic Acid ◽  
Regenerative Capacity ◽  
Leaf Segments ◽  
Ornamental Traits ◽  
Hybrid Protocols ◽  
Mitotic Inhibitor ◽  
In Vitro Shoot Regeneration

Rhododendron L.‘Fragrantissimum Improved’ is an attractive cultivar with showy, fragrant flowers but has limited potential for breeding because it is a sterile wide hybrid. Protocols for in vitro regeneration and polyploid induction were developed for this cultivar as a means to potentially restore fertility and enhance ornamental traits. Combinations of thidiazuron (TDZ) at 0, 5, 10, 15, or 20 μM and 1-naphthaleneacetic acid (NAA) at 0, 2.5, 5, or 10 μM were used to induce shoot regeneration from leaves. Shoot regeneration was optimized (68% of leaf segments produced shoots) using 8.8 μM TDZ and 10 μM NAA. To induce polyploidy, regenerative callus was treated with 7.5, 15, 30, 60, or 90 μM of the mitotic inhibitor oryzalin for 1, 3, 5, 7, or 14 d in various combinations. Oryzalin significantly affected survival and shoot regenerative capacity. A percentage of homogenous, tetraploid shoots was recovered from treatments of 30 μM oryzalin for 1 (13%) or 3 (13%) days and 7.5 μM oryzalin for 7 (20%) or 14 (7%) days.

scholarly journals Application of Artificial Neural Network for Modeling and Studying In Vitro Genotype-Independent Shoot Regeneration in Wheat

2020 ◽  
Vol 10 (15) ◽  
pp. 5370 ◽  
Author(s):  
Mohsen Hesami ◽  
Jorge A. Condori-Apfata ◽  
Maria Valderrama Valencia ◽  
Mohsen Mohammadi
Keyword(s):  
Neural Network ◽  
Shoot Regeneration ◽  
In Vitro Regeneration ◽  
Generalized Regression Neural Network ◽  
Naphthaleneacetic Acid ◽  
Regeneration Frequency ◽  
Artificial Neural ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
In Vitro Shoot Regeneration

Optimizing in vitro shoot regeneration conditions in wheat is one of the important steps in successful micropropagation and gene transformation. Various factors such as genotypes, explants, and phytohormones affect in vitro regeneration of wheat, hindering the ability to tailor genotype-independent protocols. Novel computational approaches such as artificial neural networks (ANNs) can facilitate modeling and predicting outcomes of tissue culture experiments and thereby reduce large experimental treatments and combinations. In this study, generalized regression neural network (GRNN) were used to model and forecast in vitro shoot regeneration outcomes of wheat on the basis of 10 factors including genotypes, explants, and different concentrations of 6-benzylaminopurine (BAP), kinetin (Kin), 2,4-dichlorophenoxyacetic acid (2,4-D), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), 1-naphthaleneacetic acid (NAA), zeatin, and CuSO4. In addition, GRNN was linked to a genetic algorithm (GA) to identify an optimized solution for maximum shoot regeneration. Results indicated that GRNN could accurately predict the shoot regeneration frequency in the validation set with a coefficient determination of 0.78. Sensitivity analysis demonstrated that shoot regeneration frequency was more sensitive to variables in the order of 2,4-D > explant > genotype < zeatin < NAA. Results of this study suggest that GRNN-GA can be used as a tool, besides experimental approaches, to develop and optimize in vitro genotype-independent regeneration protocols.

scholarly journals In vitro shoot regeneration from roots and leaf discs of Passiflora cincinnata mast.

2007 ◽  
Vol 50 (2) ◽  
pp. 239-247 ◽  
Author(s):  
Simone Pacheco Lombardi ◽  
Ilene Ribeiro da Silva Passos ◽  
Maria Cristina Stolf Nogueira ◽  
Beatriz Appezzato-da-Glória
Keyword(s):  
Secondary Structure ◽  
Shoot Regeneration ◽  
Callus Formation ◽  
Primary Root ◽  
Bud Formation ◽  
Leaf Discs ◽  
Root Segments ◽  
Seedling Roots ◽  
In Vitro Shoot Regeneration

Different concentrations of 6-benzyladenine (6-BA) were used in vitro to induce buds in three types of explants: leaf discs, root segments and the seedling itself obtained from in vitro seed germination of Passiflora cincinnata Mast. The 0.5 mg.L -1 6-BA concentration was most suitable for all the three explants, however, bud formation time and means (direct/indirect) were different for each type of explant. In seedlings used as explants, it had shoot regeneration from the primary root. Histological events leading to bud formation via meristemoids were described. The origin was indirect in leaf discs, with callus formation in subepidermal cells of the chlorophyll parenchyma layers. In root segments and in seedling roots, the buds were formed directly in the pericycle (in roots with some secondary structure) and in the vascular cambium (at the initial phase of secondary structure). Also, indirect buds originated from meristemoids which were formed around the callus.

In vitro shoot regeneration of mint (Mentha sp. L.) Using different types of explants and levels of benzylaminopurine

2018 ◽  
Vol 43 (4) ◽  
pp. 703-716
Author(s):  
KT Akter ◽  
MA Hoque
Keyword(s):  
Tissue Culture ◽  
Shoot Regeneration ◽  
Shoot Tip ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Different Types ◽  
Almost All ◽  
In Vitro Shoot Regeneration ◽  
Better Than

An experiment was conducted in the Tissue Culture Laboratory of the Department of Horticulture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur-1706 from March 2013 to February 2014 for in vitro shoot regeneration of mint using different explants and levels of benzylaminopurine (BAP) in full strength MS media. Three different types of explants viz. nodal segment, shoot tip and leaf were evaluated using three levels of BAP (1.0, 2.0 and 3.0 mg/l) along with control for shoot regeneration. Results revealed that shoot tip and nodal segments performed better than leaf as explants in almost all the characters studied. Shoot tip and nodal segments initiated shoot within the shortest time of 9.6 and 10.6 days, respectively with 1.0 mg/l of BAP. Regarding number of shoot per explant and number of node/shoot, shoot tip and nodal segments along with 1.0 mg/l of BAP performed superior at almost all days after inoculation. In case of interaction of explants and BAP, better performance was recorded in most of the studied parameters from shoot tip and nodal segments alongwith 1.0 mg/l BAP. Therefore, for in vitro shoot regeneration of mint, shoot tip or nodal segment may be used with 1.0 mg/l of BAP.Bangladesh J. Agril. Res. 43(4): 703-716, December 2018

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