scholarly journals IgG2 immunodeficiency: association to pediatric patients with bacterial meningoencephalitis

2000 ◽  
Vol 58 (1) ◽  
pp. 141-145 ◽  
Author(s):  
XIOMARA ESCOBAR-PÉREZ ◽  
ALBERTO J. DORTA-CONTRERAS ◽  
MARIA TERESA INTERIÁN-MORALES ◽  
ELENA NORIS-GARCÍA ◽  
MARITZA FERRÁ-VALDÉS
Keyword(s):  
Cerebrospinal Fluid ◽  
Bacterial Infections ◽  
Pediatric Patients ◽  
Haemophilus Influenzae Type ◽  
Haemophilus Influenzae Type B ◽  
Igg Subclass ◽  
Type B ◽  
Igg Subclass Deficiency ◽  
Low Levels ◽  
Intravenous Gammaglobulin

An IgG subclass deficiency is often associated with bacterial infections. We studied four pediatric patients suffering from meningoencephalitis, two of them due to Streptococcus pneumoniae and two due to Haemophilus influenzae type b. Simultaneous diagnostic serum and cerebrospinal fluid samples were taken during income. The four subclasses of IgG and albumin were quantified in both biologic fluids by radial immunodiffusion. Very low levels of seric IgG2 with non detectable cerebrospinal fluid IgG2 were found in the patients. No intrathecal IgG subclass synthesis was found in two patients. One patient with S. pneumoniae had IgG3 intrathecal synthesis. Intrathecal IgG1, IgG3 and IgG4 synthesis was found in one patient suffering from H. influenzae according with reibergrams. Substitutive therapy with intravenous gammaglobulin was given to the patients as part of the treatment.

scholarly journals Bacterial antigen detection in cerebrospinal fluid by the latex agglutination test

1995 ◽  
Vol 37 (3) ◽  
pp. 257-260 ◽  
Author(s):  
Ilka Maria Landgraf ◽  
Maria das Graças Adelino Alkmin ◽  
Maria de Fátima Paiva Vieira
Keyword(s):  
Cerebrospinal Fluid ◽  
Neisseria Meningitidis ◽  
Clinical Evidence ◽  
Latex Agglutination ◽  
Haemophilus Influenzae Type ◽  
Bacterial Antigen ◽  
Haemophilus Influenzae Type B ◽  
Type B ◽  
Gram Stain ◽  
Comparison Of The Results

Eighty purulent cerebrospinal fluid (CSF) samples from patients with clinical evidence of meningitis were studied using the Directigen latex agglutination (LA) kit to determine the presence of bacterial antigen in CSF. The results showed a better diagnostic performance of the LA test than bacterioscopy by Gram stain, culture and counterimmunoelectrophoresis (CIE), as far as Neisseria meningitidis groups B and C, and Haemophilus influenzae type b are concerned, and a better performance than bacterioscopy and culture considering Streptococcus pneumoniae. Comparison of the results with those of culture showed that the LA test had the highest sensitivity for the Neisseria meningitidis group C. Comparing the results with those of CIE, the highest levels of sensitivity were detected for N. meningitidis groups B and C. Regarding specificity, fair values were obtained for all organisms tested. The degree of K agreement when the LA test was compared with CIE exhibited better K indices of agreement for N. meningitidis groups B and C.

scholarly journals Detection of Haemophilus influenzae type b antigens in body fluids, using specific antibody-coated staphylococci

1977 ◽  
Vol 5 (1) ◽  
pp. 81-85
Author(s):  
M Suksanong ◽  
A S Dajani
Keyword(s):  
Cerebrospinal Fluid ◽  
Haemophilus Influenzae ◽  
Protein A ◽  
Body Fluids ◽  
Haemophilus Influenzae Type ◽  
Haemophilus Influenzae Type B ◽  
Bacterial Cells ◽  
Type B ◽  
Initiation Of Therapy ◽  
Urine Specimens

Protein A-rich staphylococci coated with Haemophilus influenzae type b antiserum agglutinate specifically with homologous bacterial cells or with cell-free supernatant fluids of cultures of the organism. Antibody-coated staphylococci were used to detect soluble antigens in body fluids of patients infected with H. influenzae type b. Cerebrospinal fluid from 36 cases of meningitis caused by this orgainsm showed positive coagglutination tests in 86% of patients prior to initiation of therapy. Antigens could be detected in 46% of sterile cerebrospinal fluid specimens obtained from the same cases 1 to 10 days after therapy. Soluble antigens were also detectable in sera (58%) and urine specimens (67%) of patients with H. influenzae type b septicemia, when such specimens were tested within 10 days of onset of illness. No antigen could be detected in body fluids beyond 10 days. The coagglutination test was positive in 57% of all body fluids examined; contercurrent immunoelectrophoresis (CCIE) was positive in only 27%. All specimens positive by CCIE were also positive by coagglutination. No false-positive reactions were noted by either test in body fluids from controls. The coagglutination test is simple, specific, and more sensitive than the CCIE method and could be a valuable tool for detecting antigens in body fluids of patients with various infections.

Relation of concentrations of Haemophilus influenzae type b in cerebrospinal fluid to late sequelae of patients with meningitis

1982 ◽  
Vol 100 (2) ◽  
pp. 209-212 ◽  
Author(s):  
William E. Feldman ◽  
Charles M. Ginsburg ◽  
George H. McCracken ◽  
Dolores Allen ◽  
Peter Ahmann ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Haemophilus Influenzae ◽  
Haemophilus Influenzae Type ◽  
Haemophilus Influenzae Type B ◽  
Type B ◽  
Late Sequelae

Quantitation of human IgG subclass antibodies to Haemophilus influenzae type b capsular polysaccharide

1992 ◽  
Vol 148 (1-2) ◽  
pp. 101-114 ◽  
Author(s):  
D.J. Herrmann ◽  
R.G. Hamilton ◽  
T. Barington ◽  
C.E. Frasch ◽  
G. Arakere ◽  
...  
Keyword(s):  
Haemophilus Influenzae ◽  
Capsular Polysaccharide ◽  
Haemophilus Influenzae Type ◽  
Haemophilus Influenzae Type B ◽  
Igg Subclass ◽  
Type B ◽  
Human Igg
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